RLG00000031196

pyruvate, phosphate dikinase regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013019
Physical Location & Seq
Forward (+)
2866521 .. 2867900
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031196

Sequence Viewer

Length: 1167 bp
ATGATAGCTTCTTCCACCTTCACTCTCCGGAACCTCCGCCTTTCACTCGCCGACCCCCACCGTATACCCAAACCTCCACAGACCTCATACGAACCTGGATCTGAGCCTCAGCCTCGGAAGTTCAAGGCCAGCCCTCAGCTAAGCCGATGGTCCCGGGCCCGGTCCTTGCGCTCCGGTCGGAAATTGGACCGGACCGGCCAGCAGACCCAACTACTTGACCCAAACCCTCCTCCGCAGCTCAAAGAACGAGTCTTGCCGGAGCAAAAAGACAGTTCCTTAACGGCGAGTGAGGTGGTCATGGCGGCCGGGAAGTCGATTTATATGGTTTCCGATGGAACTGGGTGGACGGTGGAACACTCCGTGGGCGCAGCGTTGGGCCAGTTTGAGCATTGCTTGGTCGATCGGAACTGCGCTGTGAATACCCATTTGTTTTCTGGGATTGATGATGTGGGGCAGTTAATGGAGATCATAAAGCAAGCAGCTAAAGAAGGAGCTATGGTGGTGTACACATTAGCCGACCCATCAATGGCTGAGTCTGCTAAACAGGCTTGTAAAATCTGGGGCTTACCCAGCATTGATATGTTAGGTCCAATTACACAGTCAATAGCTTCACATTTAAATGTGTCACCTTCTGGGGGTCCTAGGAGATACCCTCTTACAGATGAGTACTTTCAGAGGATTGAAGCGATTGAGTTTACGATCAAGCAAGATGATGGGGCATTGCCCCAGAACTTGCACAAGGCCGACATTGTGCTTGCCGGAGTTTCGAGAACGGGGAAGACCCCGTTGTCGATTTATCTCTCACAGAAGGGGTACAAGGTGGCTAATGTGCCTATTGTGATGGGGGTTCCTTTGCCAAAGACTCTTTTTGAGATTGACCCCGAGAAAGTTTTTGGCTTGACTATTAATCCTCTGGTGTTGCAAACGATTAGAAGGGCGAGGGCTAAGAGTTTGGGGTTTAGTGAAGGAGTGAGGAGTAACTACTCTGAGATGGATCATGTTCGAGAGGAGCTTGAGTATGCGCGAAAGATTCTTGCACAAAATCCTGTCTGGCCAGTAATTGAAGTGACAGGAAAAGCAATAGAAGAAACTGCAGCTGTTGTACTGAAGCTTTATCATGACAGGAAGAACAAGTGCTCAATGCCAAGAATATCGAAACGTTATTGA

Protein Analysis

389

Amino Acids

42.99

Weight (kDa)

9.32

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinase-PPPase PF03618 106 - 369 1.3e-87 Kinase/pyrophosphorylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21210 AT4G21210
fragaria_vesca FvH4_3g02680
malus_domestica MD05G1340900.v1.1
prunus_persica Prupe.4G026400_v2.0.a1
pyrus_communis pycom05g31020 pycom10g26650
rosa_chinensis RchiOBHm_Chr5g0004011
rosa_laevigata RLG00000031196
rosa_multiflora Rmu_co8460589.1_g000001 Rmu_sc0000547.1_g000016
rosa_roxburghii Rroxscaffold_1G00071960
rosa_rugosa Rorug04G0409000
rosa_samantha Rh5AG036700 Rh5BG035800 Rh5CG039400 Rh5DG035200
rosa_wichuraiana Rw5G003330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 787
AccI GTMKAC 1 cut(s) 64
AccII CGCG 1 cut(s) 1024
AccIII TCCGGA 1 cut(s) 27
AciI CCGC 3 cut(s) 37, 233, 302
AclI AACGTT 1 cut(s) 1159
AclWI GGATC 2 cut(s) 106, 1002
AcoI YGGCCR 3 cut(s) 196, 303, 1052
AcuI CTGAAG 1 cut(s) 1127
AdeI CACNNNGTG 1 cut(s) 361
AfaI GTAC 4 cut(s) 506, 668, 815, 1104
AfiI CCNNNNNNNGG 3 cut(s) 159, 526, 635
AgsI TTSAA 3 cut(s) 124, 683, 1064
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 9 cut(s) 8, 139, 238, 482, 494, 608, 1012, 1097, 1111
AluI AGCT 9 cut(s) 8, 139, 238, 482, 494, 608, 1012, 1097, 1111
Alw21I GWGCWC 1 cut(s) 1139
AlwI GGATC 2 cut(s) 106, 1002
Ama87I CYCGRG 2 cut(s) 153, 881
Aor13HI TCCGGA 1 cut(s) 27
AoxI GGCC 7 cut(s) 126, 156, 196, 303, 376, 741, 1052
ApaI GGGCCC 1 cut(s) 160
ApeKI GCWGC 4 cut(s) 235, 368, 479, 1094
ArsI GACNNNNNNTTYG 2 cut(s) 234, 266
AseI ATTAAT 1 cut(s) 906
AspA2I CCTAGG 1 cut(s) 641
AspLEI GCGC 4 cut(s) 171, 368, 413, 1024
AspS9I GGNCC 9 cut(s) 150, 156, 157, 162, 187, 192, 376, 587, 638
AsuC2I CCSGG 4 cut(s) 154, 155, 160, 307
AsuHPI GGTGA 1 cut(s) 618
AvaI CYCGRG 2 cut(s) 153, 881
AvaII GGWCC 6 cut(s) 150, 162, 187, 192, 587, 638
AvrII CCTAGG 1 cut(s) 641
BaeGI GKGCMC 1 cut(s) 160
BalI TGGCCA 1 cut(s) 1054
BanII GRGCYC 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 785
Bbv12I GWGCWC 1 cut(s) 1139
BbvCI CCTCAGC 2 cut(s) 108, 135
BbvI GCAGC 4 cut(s) 247, 380, 491, 1106
BccI CCATC 6 cut(s) 141, 326, 529, 707, 835, 985
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 1 cut(s) 96
BcnI CCSGG 4 cut(s) 154, 155, 160, 307
BfaI CTAG 1 cut(s) 642
BfmI CTRYAG 1 cut(s) 1092
BisI GCNGC 5 cut(s) 236, 303, 369, 480, 1095
BlnI CCTAGG 1 cut(s) 641
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 5 cut(s) 237, 304, 370, 481, 1096
BmcAI AGTACT 1 cut(s) 668
Bme1390I CCNGG 5 cut(s) 96, 154, 155, 160, 307
Bme18I GGWCC 6 cut(s) 150, 162, 187, 192, 587, 638
BmeT110I CYCGRG 2 cut(s) 153, 881
BmgT120I GGNCC 9 cut(s) 150, 156, 157, 162, 187, 192, 376, 587, 638
BmiI GGNNCC 5 cut(s) 32, 152, 158, 639, 849
BmrFI CCNGG 5 cut(s) 96, 154, 155, 160, 307
BmrI ACTGGG 1 cut(s) 348
BmuI ACTGGG 1 cut(s) 348
BpiI GAAGAC 1 cut(s) 785
BplI GAGNNNNNCTC 2 cut(s) 637, 669
Bpu10I CCTNAGC 2 cut(s) 108, 135
Bpu1102I GCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 1034
BpuMI CCSGG 4 cut(s) 154, 155, 160, 307
BsaJI CCNNGG 4 cut(s) 113, 153, 360, 641
BsaWI WCCGGW 3 cut(s) 27, 173, 189
BsaXI ACNNNNNCTCC 4 cut(s) 483, 513, 1001, 1031
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 526, 635
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 3 cut(s) 343, 379, 1055
Bse3DI GCAATG 2 cut(s) 388, 719
BseAI TCCGGA 1 cut(s) 27
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 4 cut(s) 113, 153, 360, 641
BseLI CCNNNNNNNGG 3 cut(s) 159, 526, 635
BseMI GCAATG 2 cut(s) 388, 719
BseMII CTCAG 5 cut(s) 93, 122, 149, 522, 978
BseNI ACTGG 3 cut(s) 343, 379, 1055
BseRI GAGGAG 3 cut(s) 219, 988, 1022
BseSI GKGCMC 1 cut(s) 160
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 4 cut(s) 247, 380, 491, 1106
BseYI CCCAGC 1 cut(s) 569
Bsh1236I CGCG 1 cut(s) 1024
Bsh1285I CGRYCG 3 cut(s) 178, 306, 403
BshFI GGCC 7 cut(s) 128, 158, 198, 305, 378, 743, 1054
BsiEI CGRYCG 3 cut(s) 178, 306, 403
BsiHKAI GWGCWC 1 cut(s) 1139
BsiHKCI CYCGRG 2 cut(s) 153, 881
BsiSI CCGG 9 cut(s) 28, 154, 160, 174, 190, 195, 257, 306, 759
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 3 cut(s) 159, 526, 635
BsmFI GGGAC 1 cut(s) 136
BsnI GGCC 7 cut(s) 128, 158, 198, 305, 378, 743, 1054
BsoBI CYCGRG 2 cut(s) 153, 881
Bsp120I GGGCCC 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 160, 1139
Bsp13I TCCGGA 1 cut(s) 27
Bsp1407I TGTACA 1 cut(s) 504
Bsp143I GATC 5 cut(s) 98, 400, 465, 699, 994
Bsp1720I GCTNAGC 1 cut(s) 140
BspACI CCGC 3 cut(s) 37, 233, 302
BspANI GGCC 7 cut(s) 128, 158, 198, 305, 378, 743, 1054
BspCNI CTCAG 5 cut(s) 94, 121, 148, 523, 979
BspEI TCCGGA 1 cut(s) 27
BspFNI CGCG 1 cut(s) 1024
BspHI TCATGA 1 cut(s) 1117
BspLI GGNNCC 5 cut(s) 32, 152, 158, 639, 849
BspMAI CTGCAG 1 cut(s) 1096
BspPI GGATC 2 cut(s) 106, 1002
BsrDI GCAATG 2 cut(s) 388, 719
BsrFI RCCGGY 1 cut(s) 194
BsrGI TGTACA 1 cut(s) 504
BsrI ACTGG 3 cut(s) 343, 379, 1055
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 4 cut(s) 113, 153, 360, 641
BssMI GATC 5 cut(s) 98, 400, 465, 699, 994
BssNAI GTATAC 1 cut(s) 65
BssT1I CCWWGG 1 cut(s) 641
Bst1107I GTATAC 1 cut(s) 65
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 4 cut(s) 62, 272, 349, 600
BstAUI TGTACA 1 cut(s) 504
BstC8I GCNNGC 4 cut(s) 130, 200, 477, 756
BstDEI CTNAG 7 cut(s) 102, 108, 135, 140, 531, 945, 987
BstDSI CCRYGG 1 cut(s) 360
BstFNI CGCG 1 cut(s) 1024
BstHHI GCGC 4 cut(s) 171, 368, 413, 1024
BstKTI GATC 5 cut(s) 101, 403, 468, 702, 997
BstMBI GATC 5 cut(s) 98, 400, 465, 699, 994
BstMCI CGRYCG 3 cut(s) 178, 306, 403
BstMWI GCNNNNNNNGC 3 cut(s) 536, 545, 570
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 5 cut(s) 94, 152, 153, 158, 305
BstSFI CTRYAG 1 cut(s) 1092
BstSLI GKGCMC 1 cut(s) 160
BstUI CGCG 1 cut(s) 1024
BstV1I GCAGC 4 cut(s) 247, 380, 491, 1106
BstV2I GAAGAC 1 cut(s) 785
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
BstZ17I GTATAC 1 cut(s) 65
BstZI CGGCCG 1 cut(s) 303
BsuRI GGCC 7 cut(s) 128, 158, 198, 305, 378, 743, 1054
BtgI CCRYGG 1 cut(s) 360
Cac8I GCNNGC 4 cut(s) 130, 200, 477, 756
CciI TCATGA 1 cut(s) 1117
CfoI GCGC 4 cut(s) 171, 368, 413, 1024
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 9 cut(s) 150, 156, 157, 162, 187, 192, 376, 587, 638
Cfr9I CCCGGG 1 cut(s) 153
CpoI CGGWCCG 1 cut(s) 192
Csp6I GTAC 4 cut(s) 505, 667, 814, 1103
CspI CGGWCCG 1 cut(s) 192
CviAII CATG 3 cut(s) 298, 998, 1118
CviQI GTAC 4 cut(s) 505, 667, 814, 1103
DdeI CTNAG 7 cut(s) 102, 108, 135, 140, 531, 945, 987
DpnI GATC 5 cut(s) 100, 402, 467, 701, 996
DpnII GATC 5 cut(s) 98, 400, 465, 699, 994
DraI TTTAAA 1 cut(s) 618
DraIII CACNNNGTG 1 cut(s) 361
DrdI GACNNNNNNGTC 1 cut(s) 787
DseDI GACNNNNNNGTC 1 cut(s) 787
EaeI YGGCCR 3 cut(s) 196, 303, 1052
EagI CGGCCG 1 cut(s) 303
EciI GGCGGA 1 cut(s) 26
EclXI CGGCCG 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 641
Eco24I GRGCYC 1 cut(s) 160
Eco47I GGWCC 6 cut(s) 150, 162, 187, 192, 587, 638
Eco52I CGGCCG 1 cut(s) 303
Eco57I CTGAAG 1 cut(s) 1127
Eco88I CYCGRG 2 cut(s) 153, 881
EcoO109I RGGNCCY 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 641
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 641
FaeI CATG 3 cut(s) 301, 1001, 1121
FaqI GGGAC 1 cut(s) 136
FatI CATG 3 cut(s) 297, 997, 1117
FblI GTMKAC 1 cut(s) 64
Fnu4HI GCNGC 5 cut(s) 236, 303, 369, 480, 1095
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 5 cut(s) 236, 303, 369, 480, 1095
FspBI CTAG 1 cut(s) 642
GlaI GCGC 4 cut(s) 170, 367, 412, 1023
GluI GCNGC 5 cut(s) 236, 303, 369, 480, 1095
GsaI CCCAGC 1 cut(s) 573
HaeIII GGCC 7 cut(s) 128, 158, 198, 305, 378, 743, 1054
HapII CCGG 9 cut(s) 28, 154, 160, 174, 190, 195, 257, 306, 759
HhaI GCGC 4 cut(s) 171, 368, 413, 1024
Hin1II CATG 3 cut(s) 301, 1001, 1121
Hin6I GCGC 4 cut(s) 169, 366, 411, 1022
HinP1I GCGC 4 cut(s) 169, 366, 411, 1022
HindIII AAGCTT 1 cut(s) 1109
HinfI GANTC 4 cut(s) 249, 533, 862, 1030
HpaII CCGG 9 cut(s) 28, 154, 160, 174, 190, 195, 257, 306, 759
HphI GGTGA 1 cut(s) 618
Hpy166II GTNNAC 5 cut(s) 65, 345, 505, 507, 696
Hpy188I TCNGA 7 cut(s) 103, 117, 180, 331, 405, 675, 988
Hpy188III TCNNGA 4 cut(s) 28, 768, 1004, 1118
Hpy8I GTNNAC 5 cut(s) 65, 345, 505, 507, 696
HpyAV CCTTC 6 cut(s) 28, 482, 639, 802, 927, 959
HpyCH4III ACNGT 4 cut(s) 62, 272, 349, 600
HpyCH4IV ACGT 1 cut(s) 1159
HpyCH4V TGCA 4 cut(s) 736, 922, 1037, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 545, 570
HpyF3I CTNAG 7 cut(s) 102, 108, 135, 140, 531, 945, 987
HpySE526I ACGT 1 cut(s) 1159
Hsp92II CATG 3 cut(s) 301, 1001, 1121
HspAI GCGC 4 cut(s) 169, 366, 411, 1022
Kpn2I TCCGGA 1 cut(s) 27
Kzo9I GATC 5 cut(s) 98, 400, 465, 699, 994
LmnI GCTCC 4 cut(s) 176, 259, 491, 1009
Lsp1109I GCAGC 4 cut(s) 247, 380, 491, 1106
MaeI CTAG 1 cut(s) 642
MaeII ACGT 1 cut(s) 1159
MaeIII GTNAC 3 cut(s) 624, 977, 1066
MalI GATC 5 cut(s) 100, 402, 467, 701, 996
MboI GATC 5 cut(s) 98, 400, 465, 699, 994
MboII GAAGA 4 cut(s) 3, 790, 1097, 1138
MflI RGATCY 1 cut(s) 98
MhlI GDGCHC 2 cut(s) 160, 1139
MlsI TGGCCA 1 cut(s) 1054
MluCI AATT 3 cut(s) 182, 591, 1059
MluNI TGGCCA 1 cut(s) 1054
MlyI GAGTC 3 cut(s) 258, 542, 856
MmeI TCCRAC 1 cut(s) 158
Mox20I TGGCCA 1 cut(s) 1054
MroI TCCGGA 1 cut(s) 27
MscI TGGCCA 1 cut(s) 1054
MseI TTAA 4 cut(s) 278, 458, 617, 906
MslI CAYNNNNRTG 2 cut(s) 578, 618
Msp20I TGGCCA 1 cut(s) 1054
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 9 cut(s) 28, 154, 160, 174, 190, 195, 257, 306, 759
MspR9I CCNGG 5 cut(s) 96, 154, 155, 160, 307
MvaI CCWGG 1 cut(s) 96
MvnI CGCG 1 cut(s) 1024
MwoI GCNNNNNNNGC 3 cut(s) 536, 545, 570
NciI CCSGG 4 cut(s) 154, 155, 160, 307
NdeII GATC 5 cut(s) 98, 400, 465, 699, 994
NlaIII CATG 3 cut(s) 301, 1001, 1121
NlaIV GGNNCC 5 cut(s) 32, 152, 158, 639, 849
NmuCI GTSAC 2 cut(s) 624, 1066
PagI TCATGA 1 cut(s) 1117
PfeI GAWTC 1 cut(s) 1030
PkrI GCNGC 5 cut(s) 237, 304, 370, 481, 1096
Ple19I CGATCG 1 cut(s) 403
PleI GAGTC 3 cut(s) 257, 541, 856
PpsI GAGTC 3 cut(s) 257, 541, 856
PpuMI RGGWCCY 1 cut(s) 638
PshBI ATTAAT 1 cut(s) 906
Psp1406I AACGTT 1 cut(s) 1159
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 5 cut(s) 32, 152, 158, 639, 849
PspOMI GGGCCC 1 cut(s) 156
PspPI GGNCC 9 cut(s) 150, 156, 157, 162, 187, 192, 376, 587, 638
PspPPI RGGWCCY 1 cut(s) 638
PstI CTGCAG 1 cut(s) 1096
PsuI RGATCY 1 cut(s) 98
PvuI CGATCG 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 1097
RsaI GTAC 4 cut(s) 506, 668, 815, 1104
RsaNI GTAC 4 cut(s) 505, 667, 814, 1103
RseI CAYNNNNRTG 2 cut(s) 578, 618
Rsr2I CGGWCCG 1 cut(s) 192
RsrII CGGWCCG 1 cut(s) 192
SaqAI TTAA 4 cut(s) 278, 458, 617, 906
SatI GCNGC 5 cut(s) 236, 303, 369, 480, 1095
Sau3AI GATC 5 cut(s) 98, 400, 465, 699, 994
Sau96I GGNCC 9 cut(s) 150, 156, 157, 162, 187, 192, 376, 587, 638
ScaI AGTACT 1 cut(s) 668
SchI GAGTC 3 cut(s) 258, 542, 856
ScrFI CCNGG 5 cut(s) 96, 154, 155, 160, 307
SduI GDGCHC 2 cut(s) 160, 1139
SfcI CTRYAG 1 cut(s) 1092
SinI GGWCC 6 cut(s) 150, 162, 187, 192, 587, 638
SmaI CCCGGG 1 cut(s) 155
SmiI ATTTAAAT 1 cut(s) 618
SmiMI CAYNNNNRTG 2 cut(s) 578, 618
SmlI CTYRAG 1 cut(s) 1013
SmoI CTYRAG 1 cut(s) 1013
Sse9I AATT 3 cut(s) 182, 591, 1059
SsiI CCGC 3 cut(s) 37, 233, 302
SspMI CTAG 1 cut(s) 642
StyD4I CCNGG 5 cut(s) 94, 152, 153, 158, 305
StyI CCWWGG 1 cut(s) 641
SwaI ATTTAAAT 1 cut(s) 618
TaaI ACNGT 4 cut(s) 62, 272, 349, 600
TaiI ACGT 1 cut(s) 1162
TaqI TCGA 6 cut(s) 314, 399, 767, 791, 1003, 1154
TasI AATT 3 cut(s) 182, 591, 1059
TatI WGTACW 3 cut(s) 504, 666, 1102
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 1 cut(s) 1030
Tru1I TTAA 4 cut(s) 278, 458, 617, 906
Tru9I TTAA 4 cut(s) 278, 458, 617, 906
TseFI GTSAC 2 cut(s) 624, 1066
TseI GCWGC 4 cut(s) 235, 368, 479, 1094
Tsp45I GTSAC 2 cut(s) 624, 1066
TspGWI ACGGA 1 cut(s) 349
TspMI CCCGGG 1 cut(s) 153
VpaK11BI GGWCC 6 cut(s) 150, 162, 187, 192, 587, 638
VspI ATTAAT 1 cut(s) 906
XcmI CCANNNNNNNNNTGG 1 cut(s) 431
XmaI CCCGGG 1 cut(s) 153
XmaJI CCTAGG 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 64
XspI CTAG 1 cut(s) 642
ZrmI AGTACT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.