Rh5AG036700

pyruvate, phosphate dikinase regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013019
Physical Location & Seq
Forward (+)
2505684 .. 2507992
2309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG036700.1

Sequence Viewer

Length: 1152 bp
ATGTCCCGTTACTACAACAAAACCAAAACCCAGGCAAAAAAATTGAACCATAAAATAATGATAGCTTCCTCCACCTTCACTCTCCGGAACCTCCGCGTCTCACTCGCCGACCCCCACCGTATACCCAAACCTCCACAGACCTCATACGAACCTGGATCCGAGCCTCAGCCTCGGAAGTTCAAGGCCAGCCCTCAGCTAAGCCGATGGTCCCGGGCCCGGTCCTTGCGCTCCGGTCGGAAATTCGACCGGACCGGCCAGCAGCCCCAACTACTCGACCCAAACCCTCCTCCGCAGCTCAAAGAACGAGTCTTGCCGGAGCAAAAAGACACTTCTTTAACGGCGAGTGACGGCGACGATGACGTGGTCATGACGACCGGGAAGTCGATTTATATGGTTTCCGACGGATCTGGGTGGACGGTGGAACACTCCGTCGGCGCAGCGTTGGGCCAGTTTGAGCATTGCTTGGTCGATCGGAACTGCGCTGTGAATACCCATTTGTTTTCTGGGATTGATGATGTGGGGCAGTTAATGGAGATTATAAAGCAAGCAGCTAAAGAAGGAGCTATGGTGGTGTACACATTAGCTGACCCATCAATGGCTGAGTCTGCTAAACAAGCTTGTAAAATTTGGGGCTTACCCAGCATTGATATGTTAGGTCCAATTACACAGTCAATAGCTTCACATCTAAATGTGTCACCTTCTGGGGGTCCTAGGAGATACCCTCTTACAGATGAGTACTTTCAGAGGATTGAAGCGATTGAGTTTACGATCAAGCAAGATGATGGGGCATTGCCCCAGAACTTACACAAGGCCGACATTGTGCTTGCCGGAGTTTCGAGAACGGGGAAGACCCCGTTGTCGATTTATCTCTCACAGAAGGGGTACAAGGTGGCTAATGTGCCTATTGTGATGGGGGTTCCTTTGCCAAAGACTCTTTTTGAGATTGACCCCGAGAAAGTTTTTGGCTTGACTATTAATCCTCTGGTGTTGCAAACGATTAGGAGGGCGAGAGCTAAGAGTTTGGGGTTCAGTGAAGGAGTGAGGAGTAACTACTCTGAGATGGATCATGTTCGAGAGGAGCTTGAGTATGCGCGAAAGATTCTTGCACAAAATCCTGTCTGGCCAGTAATTGGTAAGAACTTAGAATATTAG

Protein Analysis

383

Amino Acids

42.58

Weight (kDa)

9.27

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinase-PPPase PF03618 129 - 377 1.6e-79 Kinase/pyrophosphorylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21210 AT4G21210
fragaria_vesca FvH4_3g02680
malus_domestica MD05G1340900.v1.1
prunus_persica Prupe.4G026400_v2.0.a1
pyrus_communis pycom05g31020 pycom10g26650
rosa_chinensis RchiOBHm_Chr5g0004011
rosa_laevigata RLG00000031196
rosa_multiflora Rmu_co8460589.1_g000001 Rmu_sc0000547.1_g000016
rosa_roxburghii Rroxscaffold_1G00071960
rosa_rugosa Rorug04G0409000
rosa_samantha Rh5AG036700 Rh5BG035800 Rh5CG039400 Rh5DG035200
rosa_wichuraiana Rw5G003330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AasI GACNNNNNNGTC 2 cut(s) 379, 856
AccB7I CCANNNNNTGG 1 cut(s) 1130
AccI GTMKAC 1 cut(s) 121
AccII CGCG 2 cut(s) 96, 1093
AccIII TCCGGA 1 cut(s) 84
AciI CCGC 2 cut(s) 94, 290
AclWI GGATC 4 cut(s) 150, 163, 412, 1071
AcoI YGGCCR 2 cut(s) 253, 1121
AcsI RAATTY 2 cut(s) 239, 624
AfaI GTAC 3 cut(s) 575, 737, 884
AfiI CCNNNNNNNGG 4 cut(s) 216, 595, 704, 1130
AgsI TTSAA 3 cut(s) 46, 181, 752
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 2 cut(s) 30, 151
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 4 cut(s) 150, 163, 412, 1071
Ama87I CYCGRG 2 cut(s) 210, 950
Aor13HI TCCGGA 1 cut(s) 84
AoxI GGCC 6 cut(s) 183, 213, 253, 445, 810, 1121
ApaI GGGCCC 1 cut(s) 217
ApeKI GCWGC 4 cut(s) 259, 292, 437, 548
ApoI RAATTY 2 cut(s) 239, 624
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
AseI ATTAAT 1 cut(s) 975
AspA2I CCTAGG 1 cut(s) 710
AspLEI GCGC 4 cut(s) 228, 437, 482, 1093
AspS9I GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
AsuC2I CCSGG 4 cut(s) 211, 212, 217, 376
AsuHPI GGTGA 1 cut(s) 687
AvaI CYCGRG 2 cut(s) 210, 950
AvaII GGWCC 5 cut(s) 207, 219, 249, 656, 707
AvrII CCTAGG 1 cut(s) 710
BaeGI GKGCMC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 1123
BamHI GGATCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 854
BbvCI CCTCAGC 2 cut(s) 165, 192
BbvI GCAGC 4 cut(s) 271, 304, 449, 560
BccI CCATC 5 cut(s) 198, 598, 776, 904, 1054
BceAI ACGGC 2 cut(s) 354, 364
BciT130I CCWGG 2 cut(s) 32, 153
BcnI CCSGG 4 cut(s) 211, 212, 217, 376
BcoDI GTCTC 1 cut(s) 103
BfaI CTAG 1 cut(s) 711
BisI GCNGC 4 cut(s) 260, 293, 438, 549
BlnI CCTAGG 1 cut(s) 710
BlpI GCTNAGC 1 cut(s) 197
BlsI GCNGC 4 cut(s) 261, 294, 439, 550
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 6 cut(s) 32, 153, 211, 212, 217, 376
Bme18I GGWCC 5 cut(s) 207, 219, 249, 656, 707
BmeT110I CYCGRG 2 cut(s) 210, 950
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
BmiI GGNNCC 6 cut(s) 89, 157, 209, 215, 708, 918
BmrFI CCNGG 6 cut(s) 32, 153, 211, 212, 217, 376
BpiI GAAGAC 1 cut(s) 854
BplI GAGNNNNNCTC 2 cut(s) 706, 738
Bpu10I CCTNAGC 2 cut(s) 165, 192
Bpu1102I GCTNAGC 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 1103
BpuMI CCSGG 4 cut(s) 211, 212, 217, 376
BsaJI CCNNGG 4 cut(s) 30, 170, 210, 710
BsaWI WCCGGW 3 cut(s) 84, 230, 246
BsaXI ACNNNNNCTCC 4 cut(s) 552, 582, 1070, 1100
Bsc4I CCNNNNNNNGG 4 cut(s) 216, 595, 704, 1130
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 448, 1124
Bse3DI GCAATG 2 cut(s) 457, 788
BseAI TCCGGA 1 cut(s) 84
BseBI CCWGG 2 cut(s) 32, 153
BseDI CCNNGG 4 cut(s) 30, 170, 210, 710
BseLI CCNNNNNNNGG 4 cut(s) 216, 595, 704, 1130
BseMI GCAATG 2 cut(s) 457, 788
BseMII CTCAG 4 cut(s) 179, 206, 591, 1047
BseNI ACTGG 2 cut(s) 448, 1124
BseRI GAGGAG 3 cut(s) 276, 1057, 1091
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 4 cut(s) 271, 304, 449, 560
BseYI CCCAGC 1 cut(s) 638
Bsh1236I CGCG 2 cut(s) 96, 1093
Bsh1285I CGRYCG 4 cut(s) 235, 247, 375, 472
BshFI GGCC 6 cut(s) 185, 215, 255, 447, 812, 1123
BsiEI CGRYCG 4 cut(s) 235, 247, 375, 472
BsiHKCI CYCGRG 2 cut(s) 210, 950
BsiSI CCGG 9 cut(s) 85, 211, 217, 231, 247, 252, 314, 375, 828
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 4 cut(s) 216, 595, 704, 1130
BsmAI GTCTC 1 cut(s) 103
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 6 cut(s) 185, 215, 255, 447, 812, 1123
BsoBI CYCGRG 2 cut(s) 210, 950
Bsp120I GGGCCC 1 cut(s) 213
Bsp1286I GDGCHC 1 cut(s) 217
Bsp13I TCCGGA 1 cut(s) 84
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 5 cut(s) 155, 404, 469, 768, 1063
Bsp1720I GCTNAGC 1 cut(s) 197
BspACI CCGC 2 cut(s) 94, 290
BspANI GGCC 6 cut(s) 185, 215, 255, 447, 812, 1123
BspCNI CTCAG 4 cut(s) 178, 205, 592, 1048
BspEI TCCGGA 1 cut(s) 84
BspFNI CGCG 2 cut(s) 96, 1093
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 6 cut(s) 89, 157, 209, 215, 708, 918
BspPI GGATC 4 cut(s) 150, 163, 412, 1071
BsrDI GCAATG 2 cut(s) 457, 788
BsrFI RCCGGY 1 cut(s) 251
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 2 cut(s) 448, 1124
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 4 cut(s) 30, 170, 210, 710
BssMI GATC 5 cut(s) 155, 404, 469, 768, 1063
BssNAI GTATAC 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 710
Bst1107I GTATAC 1 cut(s) 122
Bst2UI CCWGG 2 cut(s) 32, 153
Bst4CI ACNGT 3 cut(s) 119, 418, 669
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 4 cut(s) 187, 257, 546, 825
BstDEI CTNAG 7 cut(s) 165, 192, 197, 600, 1014, 1056, 1141
BstFNI CGCG 2 cut(s) 96, 1093
BstHHI GCGC 4 cut(s) 228, 437, 482, 1093
BstKTI GATC 5 cut(s) 158, 407, 472, 771, 1066
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 5 cut(s) 155, 404, 469, 768, 1063
BstMCI CGRYCG 4 cut(s) 235, 247, 375, 472
BstMWI GCNNNNNNNGC 3 cut(s) 605, 614, 639
BstNI CCWGG 2 cut(s) 32, 153
BstSCI CCNGG 6 cut(s) 30, 151, 209, 210, 215, 374
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 2 cut(s) 96, 1093
BstV1I GCAGC 4 cut(s) 271, 304, 449, 560
BstV2I GAAGAC 1 cut(s) 854
BstX2I RGATCY 2 cut(s) 155, 404
BstYI RGATCY 2 cut(s) 155, 404
BstZ17I GTATAC 1 cut(s) 122
BsuRI GGCC 6 cut(s) 185, 215, 255, 447, 812, 1123
BtrI CACGTC 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 1036
Cac8I GCNNGC 4 cut(s) 187, 257, 546, 825
CciI TCATGA 1 cut(s) 366
CfoI GCGC 4 cut(s) 228, 437, 482, 1093
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
Cfr9I CCCGGG 1 cut(s) 210
CpoI CGGWCCG 1 cut(s) 249
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 574, 736, 883
CspI CGGWCCG 1 cut(s) 249
CviAII CATG 2 cut(s) 367, 1067
CviQI GTAC 3 cut(s) 574, 736, 883
DdeI CTNAG 7 cut(s) 165, 192, 197, 600, 1014, 1056, 1141
DpnI GATC 5 cut(s) 157, 406, 471, 770, 1065
DpnII GATC 5 cut(s) 155, 404, 469, 768, 1063
DrdI GACNNNNNNGTC 2 cut(s) 379, 856
DseDI GACNNNNNNGTC 2 cut(s) 379, 856
EaeI YGGCCR 2 cut(s) 253, 1121
Eco130I CCWWGG 1 cut(s) 710
Eco24I GRGCYC 1 cut(s) 217
Eco47I GGWCC 5 cut(s) 207, 219, 249, 656, 707
Eco88I CYCGRG 2 cut(s) 210, 950
EcoO109I RGGNCCY 1 cut(s) 707
EcoRII CCWGG 2 cut(s) 30, 151
EcoT14I CCWWGG 1 cut(s) 710
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 710
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 2 cut(s) 370, 1070
FaqI GGGAC 1 cut(s) 193
FatI CATG 2 cut(s) 366, 1066
FblI GTMKAC 1 cut(s) 121
Fnu4HI GCNGC 4 cut(s) 260, 293, 438, 549
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 4 cut(s) 260, 293, 438, 549
FspBI CTAG 1 cut(s) 711
GlaI GCGC 4 cut(s) 227, 436, 481, 1092
GluI GCNGC 4 cut(s) 260, 293, 438, 549
GsaI CCCAGC 1 cut(s) 642
HaeIII GGCC 6 cut(s) 185, 215, 255, 447, 812, 1123
HapII CCGG 9 cut(s) 85, 211, 217, 231, 247, 252, 314, 375, 828
HgaI GACGC 1 cut(s) 85
HhaI GCGC 4 cut(s) 228, 437, 482, 1093
Hin1II CATG 2 cut(s) 370, 1070
Hin6I GCGC 4 cut(s) 226, 435, 480, 1091
HinP1I GCGC 4 cut(s) 226, 435, 480, 1091
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 4 cut(s) 306, 602, 931, 1099
HpaII CCGG 9 cut(s) 85, 211, 217, 231, 247, 252, 314, 375, 828
HphI GGTGA 1 cut(s) 687
Hpy166II GTNNAC 5 cut(s) 122, 414, 574, 576, 765
Hpy188I TCNGA 7 cut(s) 160, 174, 237, 400, 474, 744, 1057
Hpy188III TCNNGA 4 cut(s) 85, 367, 837, 1073
Hpy8I GTNNAC 5 cut(s) 122, 414, 574, 576, 765
Hpy99I CGWCG 3 cut(s) 356, 404, 434
HpyAV CCTTC 5 cut(s) 85, 551, 708, 871, 1028
HpyCH4III ACNGT 3 cut(s) 119, 418, 669
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 2 cut(s) 991, 1106
HpyF10VI GCNNNNNNNGC 3 cut(s) 605, 614, 639
HpyF3I CTNAG 7 cut(s) 165, 192, 197, 600, 1014, 1056, 1141
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 2 cut(s) 370, 1070
HspAI GCGC 4 cut(s) 226, 435, 480, 1091
Kpn2I TCCGGA 1 cut(s) 84
Kzo9I GATC 5 cut(s) 155, 404, 469, 768, 1063
LmnI GCTCC 4 cut(s) 233, 316, 560, 1078
Lsp1109I GCAGC 4 cut(s) 271, 304, 449, 560
MaeI CTAG 1 cut(s) 711
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 4 cut(s) 8, 344, 693, 1046
MalI GATC 5 cut(s) 157, 406, 471, 770, 1065
MboI GATC 5 cut(s) 155, 404, 469, 768, 1063
MboII GAAGA 1 cut(s) 859
MflI RGATCY 2 cut(s) 155, 404
MhlI GDGCHC 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 1123
MluCI AATT 5 cut(s) 41, 239, 624, 660, 1128
MluNI TGGCCA 1 cut(s) 1123
MlyI GAGTC 3 cut(s) 315, 611, 925
MmeI TCCRAC 2 cut(s) 215, 423
Mox20I TGGCCA 1 cut(s) 1123
MroI TCCGGA 1 cut(s) 84
MscI TGGCCA 1 cut(s) 1123
MseI TTAA 3 cut(s) 335, 527, 975
MslI CAYNNNNRTG 2 cut(s) 647, 687
Msp20I TGGCCA 1 cut(s) 1123
MspI CCGG 9 cut(s) 85, 211, 217, 231, 247, 252, 314, 375, 828
MspR9I CCNGG 6 cut(s) 32, 153, 211, 212, 217, 376
MvaI CCWGG 2 cut(s) 32, 153
MvnI CGCG 2 cut(s) 96, 1093
MwoI GCNNNNNNNGC 3 cut(s) 605, 614, 639
NciI CCSGG 4 cut(s) 211, 212, 217, 376
NdeII GATC 5 cut(s) 155, 404, 469, 768, 1063
NlaIII CATG 2 cut(s) 370, 1070
NlaIV GGNNCC 6 cut(s) 89, 157, 209, 215, 708, 918
NmuCI GTSAC 2 cut(s) 344, 693
PagI TCATGA 1 cut(s) 366
PfeI GAWTC 1 cut(s) 1099
PflFI GACNNNGTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 1130
PkrI GCNGC 4 cut(s) 261, 294, 439, 550
Ple19I CGATCG 1 cut(s) 472
PleI GAGTC 3 cut(s) 314, 610, 925
PpsI GAGTC 3 cut(s) 314, 610, 925
PpuMI RGGWCCY 1 cut(s) 707
PshBI ATTAAT 1 cut(s) 975
PsiI TTATAA 1 cut(s) 539
Psp5II RGGWCCY 1 cut(s) 707
Psp6I CCWGG 2 cut(s) 30, 151
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 2 cut(s) 30, 151
PspN4I GGNNCC 6 cut(s) 89, 157, 209, 215, 708, 918
PspOMI GGGCCC 1 cut(s) 213
PspPI GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
PspPPI RGGWCCY 1 cut(s) 707
PsuI RGATCY 2 cut(s) 155, 404
PsyI GACNNNGTC 1 cut(s) 362
PvuI CGATCG 1 cut(s) 472
RsaI GTAC 3 cut(s) 575, 737, 884
RsaNI GTAC 3 cut(s) 574, 736, 883
RseI CAYNNNNRTG 2 cut(s) 647, 687
Rsr2I CGGWCCG 1 cut(s) 249
RsrII CGGWCCG 1 cut(s) 249
SaqAI TTAA 3 cut(s) 335, 527, 975
SatI GCNGC 4 cut(s) 260, 293, 438, 549
Sau3AI GATC 5 cut(s) 155, 404, 469, 768, 1063
Sau96I GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 3 cut(s) 315, 611, 925
ScrFI CCNGG 6 cut(s) 32, 153, 211, 212, 217, 376
SduI GDGCHC 1 cut(s) 217
SinI GGWCC 5 cut(s) 207, 219, 249, 656, 707
SmaI CCCGGG 1 cut(s) 212
SmiMI CAYNNNNRTG 2 cut(s) 647, 687
SmlI CTYRAG 1 cut(s) 1082
SmoI CTYRAG 1 cut(s) 1082
Sse9I AATT 5 cut(s) 41, 239, 624, 660, 1128
SsiI CCGC 2 cut(s) 94, 290
SspI AATATT 1 cut(s) 1148
SspMI CTAG 1 cut(s) 711
StyD4I CCNGG 6 cut(s) 30, 151, 209, 210, 215, 374
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 3 cut(s) 119, 418, 669
TaiI ACGT 1 cut(s) 363
TaqI TCGA 7 cut(s) 243, 273, 383, 468, 836, 860, 1072
TasI AATT 5 cut(s) 41, 239, 624, 660, 1128
TatI WGTACW 2 cut(s) 573, 735
TfiI GAWTC 1 cut(s) 1099
Tru1I TTAA 3 cut(s) 335, 527, 975
Tru9I TTAA 3 cut(s) 335, 527, 975
TscAI CASTG 1 cut(s) 1036
TseFI GTSAC 2 cut(s) 344, 693
TseI GCWGC 4 cut(s) 259, 292, 437, 548
Tsp45I GTSAC 2 cut(s) 344, 693
TspGWI ACGGA 2 cut(s) 417, 418
TspMI CCCGGG 1 cut(s) 210
TspRI CASTG 1 cut(s) 1036
Tth111I GACNNNGTC 1 cut(s) 362
Van91I CCANNNNNTGG 1 cut(s) 1130
VpaK11BI GGWCC 5 cut(s) 207, 219, 249, 656, 707
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 2 cut(s) 239, 624
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmaI CCCGGG 1 cut(s) 210
XmaJI CCTAGG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 121
XspI CTAG 1 cut(s) 711
ZrmI AGTACT 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.