Rh5CG039400

pyruvate, phosphate dikinase regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013019
Physical Location & Seq
Forward (+)
2506041 .. 2508112
2072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG039400.1

Sequence Viewer

Length: 1254 bp
ATGATAGCTTCCTCCACCTTCACTCTCCGGAACCTCCGCGTCTCACTCGCCGACCCCCACCGTATACCCAAACCTCCACAGACCTCATACGAACCTGGATCCGAGCCTCAGCCTCGGAAGTTCAAGGCCAGCCCTCAGCTAAGCCGATGGTCCCGGGCCCGGTCCTTGCGCTCCGGTCGGAAATTCGACCGGACCGGCCAGCAGCCCCAACTACTCGACCCAAACCCTCCTCCGCAGCTCAAAGAACGAGTCTTGCCGGAGCAAAAAGACACTTCTTTAACGGCGAGTGACGGCGACGATGACGTGGTCATGACGACCGGGAAGTCGATTTATATGGTTTCCGACGGATCTGGGTGGACGGTGGAACACTCCGTCGGCGCAGCGTTGGGCCAGTTTGAGCATTGCTTGGTCGATCGGAACTGCGCTGTGAATACCCATTTGTTTTCTGGGATTGATGATGTGGGGCAGTTAATGGAGATTATAAAGCAAGCAGCTAAAGAAGGAGCTATGGTGGTGTACACATTAGCTGACCCATCAATGGCTGAGTCTGCTAAACAAGCTTGTAAAATTTGGGGCTTACCCAGCATTGATATGTTAGGTCCAATTACACAGTCAATAGCTTCACATCTAAATGTGTCACCTTCTGGGGGTCCTAGGAGATACCCTCTTACAGATGAGTACTTTCAGAGGATTGAAGCGATTGAGTTTACGATCAAGCAAGATGATGGGGCATTGCCCCAGAACTTACACAAGGCCGACATTGTGCTTGCCGGAGTTTCGAGAACGGGGAAGACCCCGTTGTCGATTTATCTCTCACAGAAGGGGTACAAGGTGGCTAATGTGCCTATTGTGATGGGGGTTCCTTTGCCAAAGACTCTTTTTGAGATTGACCCCGAGAAAGTTTTTGGCTTGACTATTAATCCTCTGGTGTTGCAAACGATTAGGAGGGCGAGAGCTAAGAGTTTGGGGTTCAGTGAAGGAGTGAGGAGTAACTACTCTGAGATGGATCATGTTCGAGAGGAGCTTGAGTATGCGCGAAAGATTCTTGCACAAAATCCTGTCTGGCCAGTAATTGAAGTGACAGGAAAAGCAATAGAAGAAACTGCAGCTGTTGTATTGAGGCTTTATCATGACAGGAAGAACAAGTGCTCAATGCCAAGAATATCAAAACAAACTGTCAAAGTAGTAGTGTCATTATCATATTCTGGAGTGTTGGTACTGGAACTCTGGAGCAATGGAAAATGTGGCTGGTGA

Protein Analysis

417

Amino Acids

46.1

Weight (kDa)

9.01

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinase-PPPase PF03618 110 - 373 7.8e-87 Kinase/pyrophosphorylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21210 AT4G21210
fragaria_vesca FvH4_3g02680
malus_domestica MD05G1340900.v1.1
prunus_persica Prupe.4G026400_v2.0.a1
pyrus_communis pycom05g31020 pycom10g26650
rosa_chinensis RchiOBHm_Chr5g0004011
rosa_laevigata RLG00000031196
rosa_multiflora Rmu_co8460589.1_g000001 Rmu_sc0000547.1_g000016
rosa_roxburghii Rroxscaffold_1G00071960
rosa_rugosa Rorug04G0409000
rosa_samantha Rh5AG036700 Rh5BG035800 Rh5CG039400 Rh5DG035200
rosa_wichuraiana Rw5G003330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
AasI GACNNNNNNGTC 2 cut(s) 322, 799
AccI GTMKAC 1 cut(s) 64
AccII CGCG 2 cut(s) 39, 1036
AccIII TCCGGA 1 cut(s) 27
AciI CCGC 2 cut(s) 37, 233
AclWI GGATC 4 cut(s) 93, 106, 355, 1014
AcoI YGGCCR 2 cut(s) 196, 1064
AcsI RAATTY 2 cut(s) 182, 567
AfaI GTAC 4 cut(s) 518, 680, 827, 1218
AfiI CCNNNNNNNGG 3 cut(s) 159, 538, 647
AgsI TTSAA 3 cut(s) 124, 695, 1076
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 94
Alw21I GWGCWC 1 cut(s) 1151
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 4 cut(s) 93, 106, 355, 1014
Ama87I CYCGRG 2 cut(s) 153, 893
Aor13HI TCCGGA 1 cut(s) 27
AoxI GGCC 6 cut(s) 126, 156, 196, 388, 753, 1064
ApaI GGGCCC 1 cut(s) 160
ApeKI GCWGC 5 cut(s) 202, 235, 380, 491, 1106
ApoI RAATTY 2 cut(s) 182, 567
ArsI GACNNNNNNTTYG 2 cut(s) 234, 266
AseI ATTAAT 1 cut(s) 918
AspA2I CCTAGG 1 cut(s) 653
AspLEI GCGC 4 cut(s) 171, 380, 425, 1036
AspS9I GGNCC 8 cut(s) 150, 156, 157, 162, 192, 388, 599, 650
AsuC2I CCSGG 4 cut(s) 154, 155, 160, 319
AsuHPI GGTGA 1 cut(s) 630
AvaI CYCGRG 2 cut(s) 153, 893
AvaII GGWCC 5 cut(s) 150, 162, 192, 599, 650
AvrII CCTAGG 1 cut(s) 653
BaeGI GKGCMC 1 cut(s) 160
BalI TGGCCA 1 cut(s) 1066
BamHI GGATCC 1 cut(s) 98
BanII GRGCYC 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 797
Bbv12I GWGCWC 1 cut(s) 1151
BbvCI CCTCAGC 2 cut(s) 108, 135
BbvI GCAGC 5 cut(s) 214, 247, 392, 503, 1118
BccI CCATC 5 cut(s) 141, 541, 719, 847, 997
BceAI ACGGC 2 cut(s) 297, 307
BciT130I CCWGG 1 cut(s) 96
BcnI CCSGG 4 cut(s) 154, 155, 160, 319
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 654
BfmI CTRYAG 1 cut(s) 1104
BisI GCNGC 5 cut(s) 203, 236, 381, 492, 1107
BlnI CCTAGG 1 cut(s) 653
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 5 cut(s) 204, 237, 382, 493, 1108
BmcAI AGTACT 1 cut(s) 680
Bme1390I CCNGG 5 cut(s) 96, 154, 155, 160, 319
Bme18I GGWCC 5 cut(s) 150, 162, 192, 599, 650
BmeT110I CYCGRG 2 cut(s) 153, 893
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 8 cut(s) 150, 156, 157, 162, 192, 388, 599, 650
BmiI GGNNCC 6 cut(s) 32, 100, 152, 158, 651, 861
BmrFI CCNGG 5 cut(s) 96, 154, 155, 160, 319
BpiI GAAGAC 1 cut(s) 797
BplI GAGNNNNNCTC 2 cut(s) 649, 681
BpmI CTGGAG 2 cut(s) 1227, 1249
Bpu10I CCTNAGC 2 cut(s) 108, 135
Bpu1102I GCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 1046
BpuMI CCSGG 4 cut(s) 154, 155, 160, 319
BsaJI CCNNGG 3 cut(s) 113, 153, 653
BsaWI WCCGGW 3 cut(s) 27, 173, 189
BsaXI ACNNNNNCTCC 6 cut(s) 495, 525, 1013, 1043, 1200, 1230
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 538, 647
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 3 cut(s) 391, 1067, 1224
Bse3DI GCAATG 3 cut(s) 400, 731, 1240
BseAI TCCGGA 1 cut(s) 27
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 3 cut(s) 113, 153, 653
BseLI CCNNNNNNNGG 3 cut(s) 159, 538, 647
BseMI GCAATG 3 cut(s) 400, 731, 1240
BseMII CTCAG 4 cut(s) 122, 149, 534, 990
BseNI ACTGG 3 cut(s) 391, 1067, 1224
BseRI GAGGAG 3 cut(s) 219, 1000, 1034
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 5 cut(s) 214, 247, 392, 503, 1118
BseYI CCCAGC 1 cut(s) 581
Bsh1236I CGCG 2 cut(s) 39, 1036
Bsh1285I CGRYCG 4 cut(s) 178, 190, 318, 415
BshFI GGCC 6 cut(s) 128, 158, 198, 390, 755, 1066
BsiEI CGRYCG 4 cut(s) 178, 190, 318, 415
BsiHKAI GWGCWC 1 cut(s) 1151
BsiHKCI CYCGRG 2 cut(s) 153, 893
BsiSI CCGG 9 cut(s) 28, 154, 160, 174, 190, 195, 257, 318, 771
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 3 cut(s) 159, 538, 647
BsmAI GTCTC 1 cut(s) 46
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 136
BsnI GGCC 6 cut(s) 128, 158, 198, 390, 755, 1066
BsoBI CYCGRG 2 cut(s) 153, 893
Bsp120I GGGCCC 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 160, 1151
Bsp13I TCCGGA 1 cut(s) 27
Bsp1407I TGTACA 1 cut(s) 516
Bsp143I GATC 5 cut(s) 98, 347, 412, 711, 1006
Bsp1720I GCTNAGC 1 cut(s) 140
BspACI CCGC 2 cut(s) 37, 233
BspANI GGCC 6 cut(s) 128, 158, 198, 390, 755, 1066
BspCNI CTCAG 4 cut(s) 121, 148, 535, 991
BspEI TCCGGA 1 cut(s) 27
BspFNI CGCG 2 cut(s) 39, 1036
BspHI TCATGA 2 cut(s) 309, 1129
BspLI GGNNCC 6 cut(s) 32, 100, 152, 158, 651, 861
BspMAI CTGCAG 1 cut(s) 1108
BspPI GGATC 4 cut(s) 93, 106, 355, 1014
BsrDI GCAATG 3 cut(s) 400, 731, 1240
BsrFI RCCGGY 1 cut(s) 194
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 3 cut(s) 391, 1067, 1224
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 3 cut(s) 113, 153, 653
BssMI GATC 5 cut(s) 98, 347, 412, 711, 1006
BssNAI GTATAC 1 cut(s) 65
BssT1I CCWWGG 1 cut(s) 653
Bst1107I GTATAC 1 cut(s) 65
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 4 cut(s) 62, 361, 612, 1177
BstAUI TGTACA 1 cut(s) 516
BstC8I GCNNGC 4 cut(s) 130, 200, 489, 768
BstDEI CTNAG 6 cut(s) 108, 135, 140, 543, 957, 999
BstFNI CGCG 2 cut(s) 39, 1036
BstHHI GCGC 4 cut(s) 171, 380, 425, 1036
BstKTI GATC 5 cut(s) 101, 350, 415, 714, 1009
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 5 cut(s) 98, 347, 412, 711, 1006
BstMCI CGRYCG 4 cut(s) 178, 190, 318, 415
BstMWI GCNNNNNNNGC 3 cut(s) 548, 557, 582
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 5 cut(s) 94, 152, 153, 158, 317
BstSFI CTRYAG 1 cut(s) 1104
BstSLI GKGCMC 1 cut(s) 160
BstUI CGCG 2 cut(s) 39, 1036
BstV1I GCAGC 5 cut(s) 214, 247, 392, 503, 1118
BstV2I GAAGAC 1 cut(s) 797
BstX2I RGATCY 2 cut(s) 98, 347
BstYI RGATCY 2 cut(s) 98, 347
BstZ17I GTATAC 1 cut(s) 65
BsuRI GGCC 6 cut(s) 128, 158, 198, 390, 755, 1066
BtrI CACGTC 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 979
Cac8I GCNNGC 4 cut(s) 130, 200, 489, 768
CciI TCATGA 2 cut(s) 309, 1129
CfoI GCGC 4 cut(s) 171, 380, 425, 1036
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 8 cut(s) 150, 156, 157, 162, 192, 388, 599, 650
Cfr9I CCCGGG 1 cut(s) 153
CpoI CGGWCCG 1 cut(s) 192
CseI GACGC 1 cut(s) 28
Csp6I GTAC 4 cut(s) 517, 679, 826, 1217
CspI CGGWCCG 1 cut(s) 192
CviAII CATG 3 cut(s) 310, 1010, 1130
CviQI GTAC 4 cut(s) 517, 679, 826, 1217
DdeI CTNAG 6 cut(s) 108, 135, 140, 543, 957, 999
DpnI GATC 5 cut(s) 100, 349, 414, 713, 1008
DpnII GATC 5 cut(s) 98, 347, 412, 711, 1006
DrdI GACNNNNNNGTC 2 cut(s) 322, 799
DseDI GACNNNNNNGTC 2 cut(s) 322, 799
EaeI YGGCCR 2 cut(s) 196, 1064
Eco130I CCWWGG 1 cut(s) 653
Eco24I GRGCYC 1 cut(s) 160
Eco47I GGWCC 5 cut(s) 150, 162, 192, 599, 650
Eco88I CYCGRG 2 cut(s) 153, 893
EcoO109I RGGNCCY 1 cut(s) 650
EcoRII CCWGG 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 653
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 653
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 3 cut(s) 313, 1013, 1133
FaqI GGGAC 1 cut(s) 136
FatI CATG 3 cut(s) 309, 1009, 1129
FblI GTMKAC 1 cut(s) 64
Fnu4HI GCNGC 5 cut(s) 203, 236, 381, 492, 1107
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 5 cut(s) 203, 236, 381, 492, 1107
FspBI CTAG 1 cut(s) 654
GlaI GCGC 4 cut(s) 170, 379, 424, 1035
GluI GCNGC 5 cut(s) 203, 236, 381, 492, 1107
GsaI CCCAGC 1 cut(s) 585
GsuI CTGGAG 2 cut(s) 1227, 1249
HaeIII GGCC 6 cut(s) 128, 158, 198, 390, 755, 1066
HapII CCGG 9 cut(s) 28, 154, 160, 174, 190, 195, 257, 318, 771
HgaI GACGC 1 cut(s) 28
HhaI GCGC 4 cut(s) 171, 380, 425, 1036
Hin1II CATG 3 cut(s) 313, 1013, 1133
Hin6I GCGC 4 cut(s) 169, 378, 423, 1034
HinP1I GCGC 4 cut(s) 169, 378, 423, 1034
HindIII AAGCTT 1 cut(s) 558
HinfI GANTC 4 cut(s) 249, 545, 874, 1042
HpaII CCGG 9 cut(s) 28, 154, 160, 174, 190, 195, 257, 318, 771
HphI GGTGA 1 cut(s) 630
Hpy166II GTNNAC 5 cut(s) 65, 357, 517, 519, 708
Hpy188I TCNGA 7 cut(s) 103, 117, 180, 343, 417, 687, 1000
Hpy188III TCNNGA 7 cut(s) 28, 310, 780, 1016, 1130, 1206, 1228
Hpy8I GTNNAC 5 cut(s) 65, 357, 517, 519, 708
Hpy99I CGWCG 3 cut(s) 299, 347, 377
HpyAV CCTTC 5 cut(s) 28, 494, 651, 814, 971
HpyCH4III ACNGT 4 cut(s) 62, 361, 612, 1177
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 3 cut(s) 934, 1049, 1106
HpyF10VI GCNNNNNNNGC 3 cut(s) 548, 557, 582
HpyF3I CTNAG 6 cut(s) 108, 135, 140, 543, 957, 999
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 3 cut(s) 313, 1013, 1133
HspAI GCGC 4 cut(s) 169, 378, 423, 1034
Kpn2I TCCGGA 1 cut(s) 27
Kzo9I GATC 5 cut(s) 98, 347, 412, 711, 1006
LmnI GCTCC 5 cut(s) 176, 259, 503, 1021, 1230
Lsp1109I GCAGC 5 cut(s) 214, 247, 392, 503, 1118
MaeI CTAG 1 cut(s) 654
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 4 cut(s) 287, 636, 989, 1078
MalI GATC 5 cut(s) 100, 349, 414, 713, 1008
MboI GATC 5 cut(s) 98, 347, 412, 711, 1006
MboII GAAGA 3 cut(s) 802, 1109, 1150
MflI RGATCY 2 cut(s) 98, 347
MhlI GDGCHC 2 cut(s) 160, 1151
MlsI TGGCCA 1 cut(s) 1066
MluCI AATT 4 cut(s) 182, 567, 603, 1071
MluNI TGGCCA 1 cut(s) 1066
MlyI GAGTC 3 cut(s) 258, 554, 868
MmeI TCCRAC 2 cut(s) 158, 366
Mox20I TGGCCA 1 cut(s) 1066
MroI TCCGGA 1 cut(s) 27
MscI TGGCCA 1 cut(s) 1066
MseI TTAA 3 cut(s) 278, 470, 918
MslI CAYNNNNRTG 2 cut(s) 590, 630
Msp20I TGGCCA 1 cut(s) 1066
MspA1I CMGCKG 1 cut(s) 1109
MspI CCGG 9 cut(s) 28, 154, 160, 174, 190, 195, 257, 318, 771
MspR9I CCNGG 5 cut(s) 96, 154, 155, 160, 319
MvaI CCWGG 1 cut(s) 96
MvnI CGCG 2 cut(s) 39, 1036
MwoI GCNNNNNNNGC 3 cut(s) 548, 557, 582
NciI CCSGG 4 cut(s) 154, 155, 160, 319
NdeII GATC 5 cut(s) 98, 347, 412, 711, 1006
NlaIII CATG 3 cut(s) 313, 1013, 1133
NlaIV GGNNCC 6 cut(s) 32, 100, 152, 158, 651, 861
NmuCI GTSAC 3 cut(s) 287, 636, 1078
PagI TCATGA 2 cut(s) 309, 1129
PfeI GAWTC 1 cut(s) 1042
PflFI GACNNNGTC 1 cut(s) 305
PkrI GCNGC 5 cut(s) 204, 237, 382, 493, 1108
Ple19I CGATCG 1 cut(s) 415
PleI GAGTC 3 cut(s) 257, 553, 868
PpsI GAGTC 3 cut(s) 257, 553, 868
PpuMI RGGWCCY 1 cut(s) 650
PshBI ATTAAT 1 cut(s) 918
PsiI TTATAA 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 650
Psp6I CCWGG 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 6 cut(s) 32, 100, 152, 158, 651, 861
PspOMI GGGCCC 1 cut(s) 156
PspPI GGNCC 8 cut(s) 150, 156, 157, 162, 192, 388, 599, 650
PspPPI RGGWCCY 1 cut(s) 650
PstI CTGCAG 1 cut(s) 1108
PsuI RGATCY 2 cut(s) 98, 347
PsyI GACNNNGTC 1 cut(s) 305
PvuI CGATCG 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 1109
RsaI GTAC 4 cut(s) 518, 680, 827, 1218
RsaNI GTAC 4 cut(s) 517, 679, 826, 1217
RseI CAYNNNNRTG 2 cut(s) 590, 630
Rsr2I CGGWCCG 1 cut(s) 192
RsrII CGGWCCG 1 cut(s) 192
SaqAI TTAA 3 cut(s) 278, 470, 918
SatI GCNGC 5 cut(s) 203, 236, 381, 492, 1107
Sau3AI GATC 5 cut(s) 98, 347, 412, 711, 1006
Sau96I GGNCC 8 cut(s) 150, 156, 157, 162, 192, 388, 599, 650
ScaI AGTACT 1 cut(s) 680
SchI GAGTC 3 cut(s) 258, 554, 868
ScrFI CCNGG 5 cut(s) 96, 154, 155, 160, 319
SduI GDGCHC 2 cut(s) 160, 1151
SfcI CTRYAG 1 cut(s) 1104
SinI GGWCC 5 cut(s) 150, 162, 192, 599, 650
SmaI CCCGGG 1 cut(s) 155
SmiMI CAYNNNNRTG 2 cut(s) 590, 630
SmlI CTYRAG 1 cut(s) 1025
SmoI CTYRAG 1 cut(s) 1025
Sse9I AATT 4 cut(s) 182, 567, 603, 1071
SsiI CCGC 2 cut(s) 37, 233
SspMI CTAG 1 cut(s) 654
StyD4I CCNGG 5 cut(s) 94, 152, 153, 158, 317
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 4 cut(s) 62, 361, 612, 1177
TaiI ACGT 1 cut(s) 306
TaqI TCGA 7 cut(s) 186, 216, 326, 411, 779, 803, 1015
TasI AATT 4 cut(s) 182, 567, 603, 1071
TatI WGTACW 2 cut(s) 516, 678
TfiI GAWTC 1 cut(s) 1042
Tru1I TTAA 3 cut(s) 278, 470, 918
Tru9I TTAA 3 cut(s) 278, 470, 918
TscAI CASTG 1 cut(s) 979
TseFI GTSAC 3 cut(s) 287, 636, 1078
TseI GCWGC 5 cut(s) 202, 235, 380, 491, 1106
Tsp45I GTSAC 3 cut(s) 287, 636, 1078
TspGWI ACGGA 2 cut(s) 360, 361
TspMI CCCGGG 1 cut(s) 153
TspRI CASTG 1 cut(s) 979
Tth111I GACNNNGTC 1 cut(s) 305
VpaK11BI GGWCC 5 cut(s) 150, 162, 192, 599, 650
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 2 cut(s) 182, 567
XcmI CCANNNNNNNNNTGG 1 cut(s) 443
XmaI CCCGGG 1 cut(s) 153
XmaJI CCTAGG 1 cut(s) 653
XmiI GTMKAC 1 cut(s) 64
XspI CTAG 1 cut(s) 654
ZrmI AGTACT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.