RchiOBHm_Chr5g0029581

Protein trichome birefringence-like 42

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0017480
Physical Location & Seq
Reverse (-)
23187813 .. 23190518
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30896

Sequence Viewer

Length: 1137 bp
ATGCACCAAACTAATTCCCTATTCAGTTTCAGTTTTGCCAATTCCAGCAGGCAGCAGTTCACAGAATCTGAAATGGCATTCTCGTGCTTTCACCTTCTTCCGCTAGTACTACTGCCATTGACACTAAACCAAGTAGTGAATGGGAATCCAGTTTTTGATGATGGAGATCCAGTTCTCGATTATGCAGAACGAGCTGGAGTACTGATGAAGCAGCAGTCATCACATGGCTGCAATTTTTTTCAAGGGAGTTGGGTTGCTGACGATGAATACCCACTTTACAATGCTTCAAGCTGTCCATTCATTGGATTCAATTGCATAAAGAATGGTAGACCTGATAAACAGTACTTCAAATTCAATGGCCTGGATTTCATGGAAAGATACAGAGGGAAAAATATCATGTTTGTGGGAGACTCCCTAAGTAACAATATGTGGCAGTCCCTTACATGCATGCTACATGTTGCAGCCCCAAACTCCAATTACACCTTAACCCAAGCAGGGTTGCTTTCCACATTTTACCTCACGGAATATGCACTGTCGGTTATGTTTTTGAAAAATGGGTTCATAGTGGACTTGGCTTATGAAAAAATAGGCAAAGTCCTAAAACTTGACTCCATCAGCACCGGCAACCTATGGAAAGGATTAGACATGTTGATCTTCACCACATATCACTGGTGGACTCATTCCGGCCATTCTCAGACATGGGACTACTTTCAAGTGGGACAAAAGATGGTGAAAGAAATGGATCACATGGAGGCGTACAAGATTGCATTAACAACTTGGGCTAATTGGGTTGATTCCAACATTGATTTTTCAAAGACCAAAGTCTTTTTTCAAGGGGTTGCTGCAGTTCATATTGATGGAAAGGAATGGAAGGAACCAAAAGCAAGAAGTTGCAGAGGACAAACCCAACCACTGCTGGGAACTAGTTATCCAGGCCCAACTCATCCAGGAGAAGCAATTGTGAAGAGTGTATTGAGTACAGTGGCAAATCCAATCTACTTGCTTGACATTACATTGCTCACACAGCTAAGAAAAGATGGGCACCCTTCAATTTATGCCGGCCTTGGTCCCAAATTTGTGGACTGCAGTCACTGGTGTCTTCCTGGTGCTCCTGATGCTTGGAATGAACTTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

378

Amino Acids

42.68

Weight (kDa)

6.17

Isoelectric Point (pI)

28.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 76 - 118 4.4e-15 PMR5 N terminal Domain
PC-Esterase PF13839 117 - 378 9.7e-84 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017480)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78710 AT1G78710
malus_domestica MD11G1269200.v1.1
prunus_persica Prupe.4G157100_v2.0.a1 Prupe.4G157100_v2.0.a1 Prupe.4G157100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0029581
rosa_laevigata RLG00000033182
rosa_multiflora Rmu_sc0001608.1_g000004
rosa_roxburghii Rroxscaffold_1G00050350
rosa_samantha Rh5AG207400 Rh5CG228000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1041
AccB7I CCANNNNNTGG 1 cut(s) 302
AccI GTMKAC 1 cut(s) 328
AciI CCGC 1 cut(s) 101
AclWI GGATC 2 cut(s) 161, 750
AcoI YGGCCR 1 cut(s) 685
AcsI RAATTY 2 cut(s) 350, 1073
AfaI GTAC 5 cut(s) 108, 201, 344, 758, 979
AfiI CCNNNNNNNGG 3 cut(s) 302, 495, 917
AflIII ACRYGT 2 cut(s) 454, 645
AhlI ACTAGT 1 cut(s) 923
AjnI CCWGG 4 cut(s) 360, 931, 946, 1102
AloI GAACNNNNNNTCC 4 cut(s) 156, 188, 913, 945
AluBI AGCT 3 cut(s) 194, 291, 1027
AluI AGCT 3 cut(s) 194, 291, 1027
Alw21I GWGCWC 1 cut(s) 1111
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 2 cut(s) 161, 750
AlwNI CAGNNNCTG 2 cut(s) 68, 1092
AoxI GGCC 4 cut(s) 358, 685, 934, 1060
ApeKI GCWGC 5 cut(s) 52, 211, 228, 461, 842
ApoI RAATTY 2 cut(s) 350, 1073
AspS9I GGNCC 2 cut(s) 935, 1067
AsuHPI GGTGA 3 cut(s) 83, 649, 742
AvaII GGWCC 1 cut(s) 1067
BaeGI GKGCMC 1 cut(s) 1044
BanI GGYRCC 1 cut(s) 1041
BauI CACGAG 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 1091
Bbv12I GWGCWC 1 cut(s) 1111
BbvI GCAGC 5 cut(s) 64, 215, 223, 473, 829
BccI CCATC 5 cut(s) 155, 620, 721, 851, 1031
BciT130I CCWGG 4 cut(s) 362, 933, 948, 1104
BcoDI GTCTC 1 cut(s) 402
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 2 cut(s) 104, 924
BfmI CTRYAG 2 cut(s) 843, 1084
BisI GCNGC 5 cut(s) 53, 212, 229, 462, 843
BlsI GCNGC 5 cut(s) 54, 213, 230, 463, 844
BmcAI AGTACT 3 cut(s) 108, 201, 344
Bme1390I CCNGG 4 cut(s) 362, 933, 948, 1104
Bme18I GGWCC 1 cut(s) 1067
BmgT120I GGNCC 2 cut(s) 935, 1067
BmiI GGNNCC 3 cut(s) 876, 1043, 1069
BmrFI CCNGG 4 cut(s) 362, 933, 948, 1104
BmsI GCATC 1 cut(s) 1105
BoxI GACNNNNGTC 2 cut(s) 821, 1086
BpiI GAAGAC 1 cut(s) 1091
BpmI CTGGAG 1 cut(s) 216
BsaBI GATNNNNATC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 1063
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 3 cut(s) 302, 495, 917
Bse118I RCCGGY 2 cut(s) 620, 1058
Bse1I ACTGG 4 cut(s) 149, 170, 674, 1097
Bse3DI GCAATG 1 cut(s) 1013
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 4 cut(s) 362, 933, 948, 1104
BseDI CCNNGG 1 cut(s) 1063
BseGI GGATG 1 cut(s) 943
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 3 cut(s) 302, 495, 917
BseMI GCAATG 1 cut(s) 1013
BseMII CTCAG 1 cut(s) 707
BseNI ACTGG 4 cut(s) 149, 170, 674, 1097
BseSI GKGCMC 1 cut(s) 1044
BseXI GCAGC 5 cut(s) 64, 215, 223, 473, 829
BseYI CCCAGC 1 cut(s) 916
BshFI GGCC 4 cut(s) 360, 687, 936, 1062
BshNI GGYRCC 1 cut(s) 1041
BsiHKAI GWGCWC 1 cut(s) 1111
BsiSI CCGG 3 cut(s) 621, 684, 1059
BslFI GGGAC 4 cut(s) 421, 716, 732, 1053
BslI CCNNNNNNNGG 3 cut(s) 302, 495, 917
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 4 cut(s) 421, 716, 732, 1053
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 4 cut(s) 360, 687, 936, 1062
Bsp1286I GDGCHC 2 cut(s) 1044, 1111
Bsp143I GATC 3 cut(s) 166, 651, 742
BspACI CCGC 1 cut(s) 101
BspANI GGCC 4 cut(s) 360, 687, 936, 1062
BspCNI CTCAG 1 cut(s) 706
BspLI GGNNCC 3 cut(s) 876, 1043, 1069
BspMAI CTGCAG 2 cut(s) 847, 1088
BspPI GGATC 2 cut(s) 161, 750
BspT107I GGYRCC 1 cut(s) 1041
BsrDI GCAATG 1 cut(s) 1013
BsrFI RCCGGY 2 cut(s) 620, 1058
BsrI ACTGG 4 cut(s) 149, 170, 674, 1097
BssAI RCCGGY 2 cut(s) 620, 1058
BssECI CCNNGG 1 cut(s) 1063
BssMI GATC 3 cut(s) 166, 651, 742
BssSI CACGAG 1 cut(s) 82
BssT1I CCWWGG 1 cut(s) 1063
Bst2BI CACGAG 1 cut(s) 82
Bst2UI CCWGG 4 cut(s) 362, 933, 948, 1104
Bst4CI ACNGT 3 cut(s) 342, 534, 982
Bst6I CTCTTC 1 cut(s) 959
BstC8I GCNNGC 3 cut(s) 50, 449, 1060
BstDEI CTNAG 3 cut(s) 416, 693, 1028
BstF5I GGATG 1 cut(s) 943
BstKTI GATC 3 cut(s) 169, 654, 745
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 3 cut(s) 166, 651, 742
BstMWI GCNNNNNNNGC 3 cut(s) 191, 1024, 1115
BstNI CCWGG 4 cut(s) 362, 933, 948, 1104
BstNSI RCATGY 4 cut(s) 447, 451, 458, 649
BstPAI GACNNNNGTC 2 cut(s) 821, 1086
BstSCI CCNGG 4 cut(s) 360, 931, 946, 1102
BstSFI CTRYAG 2 cut(s) 843, 1084
BstSLI GKGCMC 1 cut(s) 1044
BstV1I GCAGC 5 cut(s) 64, 215, 223, 473, 829
BstV2I GAAGAC 1 cut(s) 1091
BstX2I RGATCY 1 cut(s) 166
BstXI CCANNNNNNTGG 1 cut(s) 1078
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 4 cut(s) 360, 687, 936, 1062
BtsCI GGATG 1 cut(s) 943
BtsI GCAGTG 1 cut(s) 911
BtsIMutI CAGTG 5 cut(s) 530, 667, 911, 987, 1090
Cac8I GCNNGC 3 cut(s) 50, 449, 1060
CaiI CAGNNNCTG 2 cut(s) 68, 1092
Cfr10I RCCGGY 2 cut(s) 620, 1058
Cfr13I GGNCC 2 cut(s) 935, 1067
Csp6I GTAC 5 cut(s) 107, 200, 343, 757, 978
CviAII CATG 9 cut(s) 224, 370, 397, 444, 448, 455, 646, 699, 748
CviQI GTAC 5 cut(s) 107, 200, 343, 757, 978
DdeI CTNAG 3 cut(s) 416, 693, 1028
DpnI GATC 3 cut(s) 168, 653, 744
DpnII GATC 3 cut(s) 166, 651, 742
EaeI YGGCCR 1 cut(s) 685
Eam1104I CTCTTC 1 cut(s) 959
EarI CTCTTC 1 cut(s) 959
Eco130I CCWWGG 1 cut(s) 1063
Eco47I GGWCC 1 cut(s) 1067
EcoRII CCWGG 4 cut(s) 360, 931, 946, 1102
EcoT14I CCWWGG 1 cut(s) 1063
EcoT22I ATGCAT 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 1063
FaeI CATG 9 cut(s) 227, 373, 400, 447, 451, 458, 649, 702, 751
FaqI GGGAC 4 cut(s) 421, 716, 732, 1053
FatI CATG 9 cut(s) 223, 369, 396, 443, 447, 454, 645, 698, 747
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 5 cut(s) 53, 212, 229, 462, 843
FokI GGATG 1 cut(s) 930
Fsp4HI GCNGC 5 cut(s) 53, 212, 229, 462, 843
FspBI CTAG 2 cut(s) 104, 924
GluI GCNGC 5 cut(s) 53, 212, 229, 462, 843
GsaI CCCAGC 1 cut(s) 920
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 4 cut(s) 360, 687, 936, 1062
HapII CCGG 3 cut(s) 621, 684, 1059
Hin1II CATG 9 cut(s) 227, 373, 400, 447, 451, 458, 649, 702, 751
HinfI GANTC 7 cut(s) 65, 145, 306, 410, 608, 676, 794
HpaII CCGG 3 cut(s) 621, 684, 1059
HphI GGTGA 3 cut(s) 83, 649, 742
Hpy166II GTNNAC 5 cut(s) 60, 329, 568, 675, 1081
Hpy188I TCNGA 2 cut(s) 70, 696
Hpy188III TCNNGA 2 cut(s) 176, 1112
Hpy8I GTNNAC 5 cut(s) 60, 329, 568, 675, 1081
HpyAV CCTTC 3 cut(s) 104, 865, 1056
HpyCH4III ACNGT 3 cut(s) 342, 534, 982
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 1024, 1115
HpyF3I CTNAG 3 cut(s) 416, 693, 1028
Hsp92II CATG 9 cut(s) 227, 373, 400, 447, 451, 458, 649, 702, 751
KroI GCCGGC 1 cut(s) 1058
KroNI GCCGGC 1 cut(s) 1060
Kzo9I GATC 3 cut(s) 166, 651, 742
LmnI GCTCC 1 cut(s) 1114
Lsp1109I GCAGC 5 cut(s) 64, 215, 223, 473, 829
LweI GCATC 1 cut(s) 1105
MaeI CTAG 2 cut(s) 104, 924
MaeIII GTNAC 2 cut(s) 419, 1088
MalI GATC 3 cut(s) 168, 653, 744
MboI GATC 3 cut(s) 166, 651, 742
MboII GAAGA 4 cut(s) 89, 646, 976, 1091
MfeI CAATTG 2 cut(s) 310, 957
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 2 cut(s) 1044, 1111
MlyI GAGTC 3 cut(s) 404, 602, 670
MmeI TCCRAC 1 cut(s) 822
MnlI CCTC 4 cut(s) 377, 527, 745, 890
Mph1103I ATGCAT 1 cut(s) 449
MroNI GCCGGC 1 cut(s) 1058
MseI TTAA 2 cut(s) 485, 770
MslI CAYNNNNRTG 3 cut(s) 82, 401, 855
MspI CCGG 3 cut(s) 621, 684, 1059
MspR9I CCNGG 4 cut(s) 362, 933, 948, 1104
MunI CAATTG 2 cut(s) 310, 957
Mva1269I GAATGC 1 cut(s) 77
MvaI CCWGG 4 cut(s) 362, 933, 948, 1104
MwoI GCNNNNNNNGC 3 cut(s) 191, 1024, 1115
NaeI GCCGGC 1 cut(s) 1060
NdeII GATC 3 cut(s) 166, 651, 742
NgoMIV GCCGGC 1 cut(s) 1058
NlaIII CATG 9 cut(s) 227, 373, 400, 447, 451, 458, 649, 702, 751
NlaIV GGNNCC 3 cut(s) 876, 1043, 1069
NmuCI GTSAC 1 cut(s) 1088
NsiI ATGCAT 1 cut(s) 449
NspI RCATGY 4 cut(s) 447, 451, 458, 649
PaeI GCATGC 1 cut(s) 451
PciI ACATGT 2 cut(s) 454, 645
PctI GAATGC 1 cut(s) 77
PdiI GCCGGC 1 cut(s) 1060
PfeI GAWTC 4 cut(s) 65, 145, 306, 794
PflMI CCANNNNNTGG 1 cut(s) 302
PfoI TCCNGGA 1 cut(s) 946
PkrI GCNGC 5 cut(s) 54, 213, 230, 463, 844
PleI GAGTC 3 cut(s) 404, 602, 670
PpsI GAGTC 3 cut(s) 404, 602, 670
PscI ACATGT 2 cut(s) 454, 645
PshAI GACNNNNGTC 2 cut(s) 821, 1086
Psp6I CCWGG 4 cut(s) 360, 931, 946, 1102
PspFI CCCAGC 1 cut(s) 916
PspGI CCWGG 4 cut(s) 360, 931, 946, 1102
PspN4I GGNNCC 3 cut(s) 876, 1043, 1069
PspPI GGNCC 2 cut(s) 935, 1067
PstI CTGCAG 2 cut(s) 847, 1088
PstNI CAGNNNCTG 2 cut(s) 68, 1092
PsuI RGATCY 1 cut(s) 166
RsaI GTAC 5 cut(s) 108, 201, 344, 758, 979
RsaNI GTAC 5 cut(s) 107, 200, 343, 757, 978
RseI CAYNNNNRTG 3 cut(s) 82, 401, 855
SaqAI TTAA 2 cut(s) 485, 770
SatI GCNGC 5 cut(s) 53, 212, 229, 462, 843
Sau3AI GATC 3 cut(s) 166, 651, 742
Sau96I GGNCC 2 cut(s) 935, 1067
ScaI AGTACT 3 cut(s) 108, 201, 344
SchI GAGTC 3 cut(s) 404, 602, 670
ScrFI CCNGG 4 cut(s) 362, 933, 948, 1104
SduI GDGCHC 2 cut(s) 1044, 1111
SetI ASST 8 cut(s) 96, 196, 293, 334, 485, 519, 630, 1029
SfaNI GCATC 1 cut(s) 1105
SfcI CTRYAG 2 cut(s) 843, 1084
SinI GGWCC 1 cut(s) 1067
SmiMI CAYNNNNRTG 3 cut(s) 82, 401, 855
SpeI ACTAGT 1 cut(s) 923
SphI GCATGC 1 cut(s) 451
SsiI CCGC 1 cut(s) 101
SspMI CTAG 2 cut(s) 104, 924
StyD4I CCNGG 4 cut(s) 360, 931, 946, 1102
StyI CCWWGG 1 cut(s) 1063
TaaI ACNGT 3 cut(s) 342, 534, 982
TaqI TCGA 1 cut(s) 177
TatI WGTACW 4 cut(s) 106, 199, 342, 977
TfiI GAWTC 4 cut(s) 65, 145, 306, 794
Tru1I TTAA 2 cut(s) 485, 770
Tru9I TTAA 2 cut(s) 485, 770
TscAI CASTG 5 cut(s) 537, 674, 918, 987, 1097
TseFI GTSAC 1 cut(s) 1088
TseI GCWGC 5 cut(s) 52, 211, 228, 461, 842
Tsp45I GTSAC 1 cut(s) 1088
TspDTI ATGAA 7 cut(s) 221, 279, 289, 358, 550, 594, 839
TspGWI ACGGA 1 cut(s) 536
TspRI CASTG 5 cut(s) 537, 674, 918, 987, 1097
Van91I CCANNNNNTGG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 1067
XapI RAATTY 2 cut(s) 350, 1073
XceI RCATGY 4 cut(s) 447, 451, 458, 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 328
XspI CTAG 2 cut(s) 104, 924
ZrmI AGTACT 3 cut(s) 108, 201, 344
Zsp2I ATGCAT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.