Rroxscaffold_1G00050350

Protein trichome birefringence-like 42

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017480
Physical Location & Seq
Forward (+)
70922544 .. 70924370
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00050350.1

Sequence Viewer

Length: 495 bp
ATGGCCGCCGCACTACAAACCCCAACAGAGTGTTCTGCTAGATGGGACTACTTTCAAGTGGGACAAAAGATGGTGAAAGAAATGGATCACATGGAGCCATACAAGATTGCATTAACAACATGGGCTAAATGGGTTGATTCCAACATTGATTTTTCAAAGACCAAAGTCTTTTTTCAAGGAGTTGCTGCAGTTCATATTGATGGAAAGGAATGGAAGGAACCAAAAGCAAGAAGTTGCAGAGGACAAACAATACCACTGCTGGGAGCTAGTTATCCAGGCCCAACTCATCCAGGAGAAGCAATTGTGAAGAGTGTATTGAGTACAGTGGCAAAGCCAATTTACTTGCTTGACATTACATTGCTGACACAACTAAGAAAAGATGGGCACCCTTCAATTTATGCCGGCCTTGGTCCCAAATTTGTGGACTGCAGTCACTGGTGTCTTCCTGGTGCTCCTGATGCTTGGAATGAACTTCTGTATGCAGCTTTGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

164

Amino Acids

18.2

Weight (kDa)

7.68

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 12 - 161 9.1e-46 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017480)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78710 AT1G78710
malus_domestica MD11G1269200.v1.1
prunus_persica Prupe.4G157100_v2.0.a1 Prupe.4G157100_v2.0.a1 Prupe.4G157100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0029581
rosa_laevigata RLG00000033182
rosa_multiflora Rmu_sc0001608.1_g000004
rosa_roxburghii Rroxscaffold_1G00050350
rosa_samantha Rh5AG207400 Rh5CG228000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 384
AciI CCGC 2 cut(s) 6, 9
AclWI GGATC 1 cut(s) 93
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 416
AfaI GTAC 1 cut(s) 322
AfiI CCNNNNNNNGG 1 cut(s) 260
AgsI TTSAA 4 cut(s) 56, 156, 176, 393
AjnI CCWGG 3 cut(s) 274, 289, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 16, 48
AluBI AGCT 2 cut(s) 266, 485
AluI AGCT 2 cut(s) 266, 485
Alw21I GWGCWC 1 cut(s) 454
AlwI GGATC 1 cut(s) 93
AlwNI CAGNNNCTG 1 cut(s) 435
AoxI GGCC 3 cut(s) 3, 277, 403
ApeKI GCWGC 2 cut(s) 185, 482
ApoI RAATTY 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 278, 410
AsuHPI GGTGA 1 cut(s) 85
AvaII GGWCC 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 387
BanI GGYRCC 1 cut(s) 384
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 1 cut(s) 172
BccI CCATC 4 cut(s) 36, 64, 194, 374
BciT130I CCWGG 3 cut(s) 276, 291, 447
BfaI CTAG 3 cut(s) 39, 267, 493
BfmI CTRYAG 2 cut(s) 186, 427
BisI GCNGC 4 cut(s) 6, 9, 186, 483
BlsI GCNGC 4 cut(s) 7, 10, 187, 484
Bme1390I CCNGG 3 cut(s) 276, 291, 447
Bme18I GGWCC 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 278, 410
BmiI GGNNCC 4 cut(s) 96, 219, 386, 412
BmrFI CCNGG 3 cut(s) 276, 291, 447
BmsI GCATC 1 cut(s) 448
BoxI GACNNNNGTC 2 cut(s) 164, 429
BpiI GAAGAC 1 cut(s) 434
BsaJI CCNNGG 1 cut(s) 406
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse118I RCCGGY 1 cut(s) 401
Bse1I ACTGG 1 cut(s) 440
Bse3DI GCAATG 1 cut(s) 356
BseBI CCWGG 3 cut(s) 276, 291, 447
BseDI CCNNGG 1 cut(s) 406
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 440
BseSI GKGCMC 1 cut(s) 387
BseXI GCAGC 1 cut(s) 172
BseYI CCCAGC 1 cut(s) 259
BshFI GGCC 3 cut(s) 5, 279, 405
BshNI GGYRCC 1 cut(s) 384
BsiHKAI GWGCWC 1 cut(s) 454
BsiSI CCGG 1 cut(s) 402
BslFI GGGAC 3 cut(s) 59, 75, 396
BslI CCNNNNNNNGG 1 cut(s) 260
BsmFI GGGAC 3 cut(s) 59, 75, 396
BsnI GGCC 3 cut(s) 5, 279, 405
Bsp1286I GDGCHC 2 cut(s) 387, 454
Bsp143I GATC 1 cut(s) 85
BspACI CCGC 2 cut(s) 6, 9
BspANI GGCC 3 cut(s) 5, 279, 405
BspLI GGNNCC 4 cut(s) 96, 219, 386, 412
BspMAI CTGCAG 2 cut(s) 190, 431
BspPI GGATC 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 401
BsrI ACTGG 1 cut(s) 440
BssAI RCCGGY 1 cut(s) 401
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 1 cut(s) 85
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 3 cut(s) 276, 291, 447
Bst4CI ACNGT 1 cut(s) 325
Bst6I CTCTTC 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 286
BstKTI GATC 1 cut(s) 88
BstMBI GATC 1 cut(s) 85
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 3 cut(s) 276, 291, 447
BstPAI GACNNNNGTC 2 cut(s) 164, 429
BstSCI CCNGG 3 cut(s) 274, 289, 445
BstSFI CTRYAG 2 cut(s) 186, 427
BstSLI GKGCMC 1 cut(s) 387
BstV1I GCAGC 1 cut(s) 172
BstV2I GAAGAC 1 cut(s) 434
BstXI CCANNNNNNTGG 1 cut(s) 421
BsuRI GGCC 3 cut(s) 5, 279, 405
BtsCI GGATG 1 cut(s) 286
BtsI GCAGTG 1 cut(s) 254
BtsIMutI CAGTG 3 cut(s) 254, 330, 433
Cac8I GCNNGC 1 cut(s) 403
CaiI CAGNNNCTG 1 cut(s) 435
Cfr10I RCCGGY 1 cut(s) 401
Cfr13I GGNCC 2 cut(s) 278, 410
Csp6I GTAC 1 cut(s) 321
CviAII CATG 2 cut(s) 91, 120
CviJI RGCY 8 cut(s) 5, 97, 125, 266, 279, 334, 405, 485
CviKI_1 RGCY 8 cut(s) 5, 97, 125, 266, 279, 334, 405, 485
CviQI GTAC 1 cut(s) 321
DdeI CTNAG 1 cut(s) 371
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
Eco130I CCWWGG 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 410
EcoRII CCWGG 3 cut(s) 274, 289, 445
EcoT14I CCWWGG 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 2 cut(s) 94, 123
FaiI YATR 6 cut(s) 92, 100, 121, 195, 399, 480
FaqI GGGAC 3 cut(s) 59, 75, 396
FatI CATG 2 cut(s) 90, 119
Fnu4HI GCNGC 4 cut(s) 6, 9, 186, 483
FokI GGATG 1 cut(s) 273
Fsp4HI GCNGC 4 cut(s) 6, 9, 186, 483
FspBI CTAG 3 cut(s) 39, 267, 493
GluI GCNGC 4 cut(s) 6, 9, 186, 483
GsaI CCCAGC 1 cut(s) 263
HaeIII GGCC 3 cut(s) 5, 279, 405
HapII CCGG 1 cut(s) 402
Hin1II CATG 2 cut(s) 94, 123
HinfI GANTC 1 cut(s) 137
HpaII CCGG 1 cut(s) 402
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 424
Hpy188III TCNNGA 1 cut(s) 455
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 2 cut(s) 208, 399
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4V TGCA 5 cut(s) 110, 188, 237, 429, 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 1 cut(s) 371
Hsp92II CATG 2 cut(s) 94, 123
KroI GCCGGC 1 cut(s) 401
KroNI GCCGGC 1 cut(s) 403
Kzo9I GATC 1 cut(s) 85
LmnI GCTCC 3 cut(s) 94, 263, 457
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 1 cut(s) 448
MaeI CTAG 3 cut(s) 39, 267, 493
MaeIII GTNAC 1 cut(s) 431
MalI GATC 1 cut(s) 87
MboI GATC 1 cut(s) 85
MboII GAAGA 2 cut(s) 319, 434
MfeI CAATTG 1 cut(s) 300
MhlI GDGCHC 2 cut(s) 387, 454
MluCI AATT 4 cut(s) 300, 336, 393, 416
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 1 cut(s) 233
MroNI GCCGGC 1 cut(s) 401
MseI TTAA 1 cut(s) 113
MslI CAYNNNNRTG 1 cut(s) 198
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 3 cut(s) 276, 291, 447
MunI CAATTG 1 cut(s) 300
MvaI CCWGG 3 cut(s) 276, 291, 447
MwoI GCNNNNNNNGC 1 cut(s) 458
NaeI GCCGGC 1 cut(s) 403
NdeII GATC 1 cut(s) 85
NgoMIV GCCGGC 1 cut(s) 401
NlaIII CATG 2 cut(s) 94, 123
NlaIV GGNNCC 4 cut(s) 96, 219, 386, 412
NmuCI GTSAC 1 cut(s) 431
PdiI GCCGGC 1 cut(s) 403
PfeI GAWTC 1 cut(s) 137
PfoI TCCNGGA 1 cut(s) 289
PkrI GCNGC 4 cut(s) 7, 10, 187, 484
PshAI GACNNNNGTC 2 cut(s) 164, 429
Psp6I CCWGG 3 cut(s) 274, 289, 445
PspFI CCCAGC 1 cut(s) 259
PspGI CCWGG 3 cut(s) 274, 289, 445
PspN4I GGNNCC 4 cut(s) 96, 219, 386, 412
PspPI GGNCC 2 cut(s) 278, 410
PstI CTGCAG 2 cut(s) 190, 431
PstNI CAGNNNCTG 1 cut(s) 435
RsaI GTAC 1 cut(s) 322
RsaNI GTAC 1 cut(s) 321
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 4 cut(s) 6, 9, 186, 483
Sau3AI GATC 1 cut(s) 85
Sau96I GGNCC 2 cut(s) 278, 410
ScrFI CCNGG 3 cut(s) 276, 291, 447
SduI GDGCHC 2 cut(s) 387, 454
SetI ASST 2 cut(s) 268, 487
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 2 cut(s) 186, 427
SinI GGWCC 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 198
Sse9I AATT 4 cut(s) 300, 336, 393, 416
SsiI CCGC 2 cut(s) 6, 9
SspMI CTAG 3 cut(s) 39, 267, 493
StyD4I CCNGG 3 cut(s) 274, 289, 445
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 1 cut(s) 325
TasI AATT 4 cut(s) 300, 336, 393, 416
TatI WGTACW 1 cut(s) 320
TauI GCSGC 2 cut(s) 8, 11
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 3 cut(s) 261, 330, 440
TseFI GTSAC 1 cut(s) 431
TseI GCWGC 2 cut(s) 185, 482
Tsp45I GTSAC 1 cut(s) 431
TspDTI ATGAA 2 cut(s) 182, 483
TspRI CASTG 3 cut(s) 261, 330, 440
VpaK11BI GGWCC 1 cut(s) 410
XapI RAATTY 1 cut(s) 416
XspI CTAG 3 cut(s) 39, 267, 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.