Rh5CG228000

Protein trichome birefringence-like 42

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017480
Physical Location & Seq
Reverse (-)
23283339 .. 23289856
6518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG228000.1

Sequence Viewer

Length: 1113 bp
ATGGCATTCTCGTGCTTTCACCTTCTTCCTCTAGTACTACTGCCATTGACACTAAACCAAGTAGTGAATGGGAATCCAGTTTTTGATGATGGAGATTCAGTTCTCGATTATGCAGAAGGAGCTGGAGTACTGATGAAGCAGTCATCACATGGCTGCAATTTTTTTCAAGGGAGTTGGGTTGCTGACGATGAATACCCACTTTACATTGCTTCAAGCTGTCCATTCATTGGATTCAATTGCATAAAGAATGGTAGACCTGATAAACAGTACTTCAAATATAGATGGAAGCCTACTGGTTGTGACCTTCCAAGATTCAATGGCCTGGATTTCATGGAAAGATACAGAGGGAAAAAGATAATGTTTGTGGGAGACTCCCTAAGTAACAATATGTGGCAGTCCCTTACATGCATGCTACATGTTGCAGCCCCAAACTCCAATTACACCTTAATCCAAGCAGGGTTGCTTTCCACATTTTACCTCAAGGAATATGCACTGTCGGTTATGTTTTTGAAAAATGGGTTCATAGTGGACTTGGCTTATGAAAAAATAGGCAAAGTCCTAAAACTTGACTCCATCAGCACCGGCAACCTGTGGAAAGGAGTAGACATGTTGATCTTCACCACATATCACTGGTGGACTCATTCCGGCCATTCTCAGACATGGGACTACTTTCAAGTGGGACAAAAGATGGTGAAAGAAATGGATCACATGGAGGCATACAAGATTGCATTAACAACTTGGGCTGAATGGGTTGATTCCAACATTGATTTTTCAAAGACCAAAGTCTTTTTTCAAGGAGTTGCTGCAGTTCATATTGATGGAAAGGAATGGAAGGAACCAAAAGCAAGAAGTTGCAGAGGACAAACAAAACCACTGCTGGGAGCTAGTTATCCAGGCCCAACTCATCCAGGAGAAGCAATTGTGAAGAGTGTATTGAGTACAGTGGCAAATCCAATTTACTTGCTTGACATTACATTGCTGACACAGCTAAGAAAAGATGGGCACCCTTCAATTTATGCCGGCCTTGGTCCCAAATTTGTGGACTGCAGTCACTGGTGTCTTCCTGGTACTCCTGATGCTTGGAATGAACTTCTGTATGCAGCTTTGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

370

Amino Acids

41.71

Weight (kDa)

6.82

Isoelectric Point (pI)

21.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 51 - 101 1.1e-20 PMR5 N terminal Domain
PC-Esterase PF13839 102 - 367 1e-86 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017480)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78710 AT1G78710
malus_domestica MD11G1269200.v1.1
prunus_persica Prupe.4G157100_v2.0.a1 Prupe.4G157100_v2.0.a1 Prupe.4G157100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0029581
rosa_laevigata RLG00000033182
rosa_multiflora Rmu_sc0001608.1_g000004
rosa_roxburghii Rroxscaffold_1G00050350
rosa_samantha Rh5AG207400 Rh5CG228000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1002
AccB7I CCANNNNNTGG 1 cut(s) 227
AccI GTMKAC 2 cut(s) 253, 603
AclWI GGATC 1 cut(s) 711
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 1 cut(s) 1034
AfaI GTAC 5 cut(s) 36, 129, 269, 940, 1069
AfiI CCNNNNNNNGG 2 cut(s) 227, 878
AflIII ACRYGT 2 cut(s) 415, 606
AjnI CCWGG 4 cut(s) 321, 892, 907, 1063
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
AluBI AGCT 5 cut(s) 122, 216, 884, 988, 1103
AluI AGCT 5 cut(s) 122, 216, 884, 988, 1103
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 1 cut(s) 711
AlwNI CAGNNNCTG 1 cut(s) 1053
AoxI GGCC 4 cut(s) 319, 646, 895, 1021
ApeKI GCWGC 4 cut(s) 153, 422, 803, 1100
ApoI RAATTY 1 cut(s) 1034
AspS9I GGNCC 2 cut(s) 896, 1028
AsuHPI GGTGA 3 cut(s) 11, 610, 703
AvaII GGWCC 1 cut(s) 1028
BaeGI GKGCMC 1 cut(s) 1005
BanI GGYRCC 1 cut(s) 1002
BauI CACGAG 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 1052
BbvI GCAGC 3 cut(s) 140, 434, 790
BccI CCATC 6 cut(s) 83, 276, 581, 682, 812, 992
BciT130I CCWGG 4 cut(s) 323, 894, 909, 1065
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 3 cut(s) 32, 885, 1111
BfmI CTRYAG 2 cut(s) 804, 1045
BisI GCNGC 4 cut(s) 154, 423, 804, 1101
BlsI GCNGC 4 cut(s) 155, 424, 805, 1102
BmcAI AGTACT 3 cut(s) 36, 129, 269
Bme1390I CCNGG 4 cut(s) 323, 894, 909, 1065
Bme18I GGWCC 1 cut(s) 1028
BmgT120I GGNCC 2 cut(s) 896, 1028
BmiI GGNNCC 3 cut(s) 837, 1004, 1030
BmrFI CCNGG 4 cut(s) 323, 894, 909, 1065
BmsI GCATC 1 cut(s) 1066
BoxI GACNNNNGTC 2 cut(s) 782, 1047
BpiI GAAGAC 1 cut(s) 1052
BpmI CTGGAG 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 1024
BsaXI ACNNNNNCTCC 4 cut(s) 84, 111, 114, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 878
Bse118I RCCGGY 2 cut(s) 581, 1019
Bse1I ACTGG 4 cut(s) 77, 298, 635, 1058
Bse3DI GCAATG 2 cut(s) 204, 974
BseBI CCWGG 4 cut(s) 323, 894, 909, 1065
BseDI CCNNGG 1 cut(s) 1024
BseGI GGATG 1 cut(s) 904
BseLI CCNNNNNNNGG 2 cut(s) 227, 878
BseMI GCAATG 2 cut(s) 204, 974
BseMII CTCAG 1 cut(s) 668
BseNI ACTGG 4 cut(s) 77, 298, 635, 1058
BseSI GKGCMC 1 cut(s) 1005
BseXI GCAGC 3 cut(s) 140, 434, 790
BseYI CCCAGC 1 cut(s) 877
BshFI GGCC 4 cut(s) 321, 648, 897, 1023
BshNI GGYRCC 1 cut(s) 1002
BsiSI CCGG 3 cut(s) 582, 645, 1020
BslFI GGGAC 4 cut(s) 382, 677, 693, 1014
BslI CCNNNNNNNGG 2 cut(s) 227, 878
BsmAI GTCTC 1 cut(s) 363
BsmFI GGGAC 4 cut(s) 382, 677, 693, 1014
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 4 cut(s) 321, 648, 897, 1023
Bsp1286I GDGCHC 1 cut(s) 1005
Bsp143I GATC 2 cut(s) 612, 703
BspANI GGCC 4 cut(s) 321, 648, 897, 1023
BspCNI CTCAG 1 cut(s) 667
BspLI GGNNCC 3 cut(s) 837, 1004, 1030
BspMAI CTGCAG 2 cut(s) 808, 1049
BspPI GGATC 1 cut(s) 711
BspT107I GGYRCC 1 cut(s) 1002
BsrDI GCAATG 2 cut(s) 204, 974
BsrFI RCCGGY 2 cut(s) 581, 1019
BsrI ACTGG 4 cut(s) 77, 298, 635, 1058
BssAI RCCGGY 2 cut(s) 581, 1019
BssECI CCNNGG 1 cut(s) 1024
BssMI GATC 2 cut(s) 612, 703
BssSI CACGAG 1 cut(s) 10
BssT1I CCWWGG 1 cut(s) 1024
Bst2BI CACGAG 1 cut(s) 10
Bst2UI CCWGG 4 cut(s) 323, 894, 909, 1065
Bst4CI ACNGT 3 cut(s) 267, 495, 943
Bst6I CTCTTC 1 cut(s) 920
BstC8I GCNNGC 2 cut(s) 410, 1021
BstDEI CTNAG 3 cut(s) 377, 654, 989
BstF5I GGATG 1 cut(s) 904
BstKTI GATC 2 cut(s) 615, 706
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 2 cut(s) 612, 703
BstMWI GCNNNNNNNGC 2 cut(s) 119, 985
BstNI CCWGG 4 cut(s) 323, 894, 909, 1065
BstNSI RCATGY 4 cut(s) 408, 412, 419, 610
BstPAI GACNNNNGTC 2 cut(s) 782, 1047
BstSCI CCNGG 4 cut(s) 321, 892, 907, 1063
BstSFI CTRYAG 2 cut(s) 804, 1045
BstSLI GKGCMC 1 cut(s) 1005
BstV1I GCAGC 3 cut(s) 140, 434, 790
BstV2I GAAGAC 1 cut(s) 1052
BstXI CCANNNNNNTGG 1 cut(s) 1039
BsuRI GGCC 4 cut(s) 321, 648, 897, 1023
BtsCI GGATG 1 cut(s) 904
BtsI GCAGTG 1 cut(s) 872
BtsIMutI CAGTG 5 cut(s) 491, 628, 872, 948, 1051
Cac8I GCNNGC 2 cut(s) 410, 1021
CaiI CAGNNNCTG 1 cut(s) 1053
Cfr10I RCCGGY 2 cut(s) 581, 1019
Cfr13I GGNCC 2 cut(s) 896, 1028
Csp6I GTAC 5 cut(s) 35, 128, 268, 939, 1068
CviAII CATG 8 cut(s) 149, 331, 405, 409, 416, 607, 660, 709
CviQI GTAC 5 cut(s) 35, 128, 268, 939, 1068
DdeI CTNAG 3 cut(s) 377, 654, 989
DpnI GATC 2 cut(s) 614, 705
DpnII GATC 2 cut(s) 612, 703
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 1 cut(s) 920
EarI CTCTTC 1 cut(s) 920
Eco130I CCWWGG 1 cut(s) 1024
Eco47I GGWCC 1 cut(s) 1028
EcoRII CCWGG 4 cut(s) 321, 892, 907, 1063
EcoT14I CCWWGG 1 cut(s) 1024
EcoT22I ATGCAT 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 1024
FaeI CATG 8 cut(s) 152, 334, 408, 412, 419, 610, 663, 712
FaqI GGGAC 4 cut(s) 382, 677, 693, 1014
FatI CATG 8 cut(s) 148, 330, 404, 408, 415, 606, 659, 708
FblI GTMKAC 2 cut(s) 253, 603
Fnu4HI GCNGC 4 cut(s) 154, 423, 804, 1101
FokI GGATG 1 cut(s) 891
Fsp4HI GCNGC 4 cut(s) 154, 423, 804, 1101
FspBI CTAG 3 cut(s) 32, 885, 1111
GluI GCNGC 4 cut(s) 154, 423, 804, 1101
GsaI CCCAGC 1 cut(s) 881
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 4 cut(s) 321, 648, 897, 1023
HapII CCGG 3 cut(s) 582, 645, 1020
Hin1II CATG 8 cut(s) 152, 334, 408, 412, 419, 610, 663, 712
HinfI GANTC 8 cut(s) 73, 95, 231, 312, 371, 569, 637, 755
HpaII CCGG 3 cut(s) 582, 645, 1020
HphI GGTGA 3 cut(s) 11, 610, 703
Hpy166II GTNNAC 5 cut(s) 254, 529, 604, 636, 1042
Hpy188I TCNGA 1 cut(s) 657
Hpy188III TCNNGA 2 cut(s) 104, 1073
Hpy8I GTNNAC 5 cut(s) 254, 529, 604, 636, 1042
HpyAV CCTTC 5 cut(s) 32, 110, 314, 826, 1017
HpyCH4III ACNGT 3 cut(s) 267, 495, 943
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 985
HpyF3I CTNAG 3 cut(s) 377, 654, 989
Hsp92II CATG 8 cut(s) 152, 334, 408, 412, 419, 610, 663, 712
KroI GCCGGC 1 cut(s) 1019
KroNI GCCGGC 1 cut(s) 1021
Kzo9I GATC 2 cut(s) 612, 703
LmnI GCTCC 2 cut(s) 119, 881
Lsp1109I GCAGC 3 cut(s) 140, 434, 790
LweI GCATC 1 cut(s) 1066
MaeI CTAG 3 cut(s) 32, 885, 1111
MaeIII GTNAC 3 cut(s) 299, 380, 1049
MalI GATC 2 cut(s) 614, 705
MboI GATC 2 cut(s) 612, 703
MboII GAAGA 4 cut(s) 17, 607, 937, 1052
MfeI CAATTG 2 cut(s) 235, 918
MhlI GDGCHC 1 cut(s) 1005
MluCI AATT 7 cut(s) 157, 235, 436, 918, 954, 1011, 1034
MlyI GAGTC 3 cut(s) 365, 563, 631
MmeI TCCRAC 1 cut(s) 783
MnlI CCTC 5 cut(s) 39, 338, 488, 706, 851
Mph1103I ATGCAT 1 cut(s) 410
MroNI GCCGGC 1 cut(s) 1019
MseI TTAA 2 cut(s) 446, 731
MslI CAYNNNNRTG 2 cut(s) 10, 816
MspI CCGG 3 cut(s) 582, 645, 1020
MspR9I CCNGG 4 cut(s) 323, 894, 909, 1065
MunI CAATTG 2 cut(s) 235, 918
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 4 cut(s) 323, 894, 909, 1065
MwoI GCNNNNNNNGC 2 cut(s) 119, 985
NaeI GCCGGC 1 cut(s) 1021
NdeII GATC 2 cut(s) 612, 703
NgoMIV GCCGGC 1 cut(s) 1019
NlaIII CATG 8 cut(s) 152, 334, 408, 412, 419, 610, 663, 712
NlaIV GGNNCC 3 cut(s) 837, 1004, 1030
NmuCI GTSAC 2 cut(s) 299, 1049
NsiI ATGCAT 1 cut(s) 410
NspI RCATGY 4 cut(s) 408, 412, 419, 610
PaeI GCATGC 1 cut(s) 412
PciI ACATGT 2 cut(s) 415, 606
PctI GAATGC 1 cut(s) 5
PdiI GCCGGC 1 cut(s) 1021
PfeI GAWTC 5 cut(s) 73, 95, 231, 312, 755
PflMI CCANNNNNTGG 1 cut(s) 227
PfoI TCCNGGA 1 cut(s) 907
PkrI GCNGC 4 cut(s) 155, 424, 805, 1102
PleI GAGTC 3 cut(s) 365, 563, 631
PpsI GAGTC 3 cut(s) 365, 563, 631
PscI ACATGT 2 cut(s) 415, 606
PshAI GACNNNNGTC 2 cut(s) 782, 1047
Psp6I CCWGG 4 cut(s) 321, 892, 907, 1063
PspFI CCCAGC 1 cut(s) 877
PspGI CCWGG 4 cut(s) 321, 892, 907, 1063
PspN4I GGNNCC 3 cut(s) 837, 1004, 1030
PspPI GGNCC 2 cut(s) 896, 1028
PstI CTGCAG 2 cut(s) 808, 1049
PstNI CAGNNNCTG 1 cut(s) 1053
RsaI GTAC 5 cut(s) 36, 129, 269, 940, 1069
RsaNI GTAC 5 cut(s) 35, 128, 268, 939, 1068
RseI CAYNNNNRTG 2 cut(s) 10, 816
SaqAI TTAA 2 cut(s) 446, 731
SatI GCNGC 4 cut(s) 154, 423, 804, 1101
Sau3AI GATC 2 cut(s) 612, 703
Sau96I GGNCC 2 cut(s) 896, 1028
ScaI AGTACT 3 cut(s) 36, 129, 269
SchI GAGTC 3 cut(s) 365, 563, 631
ScrFI CCNGG 4 cut(s) 323, 894, 909, 1065
SduI GDGCHC 1 cut(s) 1005
SfaNI GCATC 1 cut(s) 1066
SfcI CTRYAG 2 cut(s) 804, 1045
SinI GGWCC 1 cut(s) 1028
SmiMI CAYNNNNRTG 2 cut(s) 10, 816
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SphI GCATGC 1 cut(s) 412
Sse9I AATT 7 cut(s) 157, 235, 436, 918, 954, 1011, 1034
SspMI CTAG 3 cut(s) 32, 885, 1111
StyD4I CCNGG 4 cut(s) 321, 892, 907, 1063
StyI CCWWGG 1 cut(s) 1024
TaaI ACNGT 3 cut(s) 267, 495, 943
TaqI TCGA 1 cut(s) 105
TasI AATT 7 cut(s) 157, 235, 436, 918, 954, 1011, 1034
TatI WGTACW 4 cut(s) 34, 127, 267, 938
TfiI GAWTC 5 cut(s) 73, 95, 231, 312, 755
Tru1I TTAA 2 cut(s) 446, 731
Tru9I TTAA 2 cut(s) 446, 731
TscAI CASTG 5 cut(s) 498, 635, 879, 948, 1058
TseFI GTSAC 2 cut(s) 299, 1049
TseI GCWGC 4 cut(s) 153, 422, 803, 1100
Tsp45I GTSAC 2 cut(s) 299, 1049
TspDTI ATGAA 8 cut(s) 149, 204, 214, 319, 511, 555, 800, 1101
TspRI CASTG 5 cut(s) 498, 635, 879, 948, 1058
Van91I CCANNNNNTGG 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 1028
XapI RAATTY 1 cut(s) 1034
XceI RCATGY 4 cut(s) 408, 412, 419, 610
XcmI CCANNNNNNNNNTGG 1 cut(s) 65
XmiI GTMKAC 2 cut(s) 253, 603
XspI CTAG 3 cut(s) 32, 885, 1111
ZrmI AGTACT 3 cut(s) 36, 129, 269
Zsp2I ATGCAT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.