RchiOBHm_Chr5g0041441

Inorganic pyrophosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010511
Physical Location & Seq
Reverse (-)
36300916 .. 36301206
291 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31988

Sequence Viewer

Length: 291 bp
ATGCATCCACAAGTAGCTGCAGCTATTAAGTCAGCTCATGCTTCGGGATGTGACTTAAAGATAATTAGTGATGCAAATCAGTTTTTCATTGAGTCAATCTTGGAATGTTGTGGCCTGTTGGTATGCTTCTCACAGATCATCACAAACCCAACATCTGTAGATGGGGATGGAAGACTGAGAATCTTTCCTTACCATGATCCAGTCTCATCTTCTCATGGTTGCAATCTTTGCCCTTCAAATATGTGCAAGGGTCCGGTGATTGACCAATTTTTAGCTTCTAGCTGCTTCTGA

Protein Analysis

96

Amino Acids

10.29

Weight (kDa)

5.63

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Put_Phosphatase PF06888 2 - 93 9e-33 Putative Phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 191
AgsI TTSAA 1 cut(s) 237
AluBI AGCT 5 cut(s) 17, 23, 35, 275, 282
AluI AGCT 5 cut(s) 17, 23, 35, 275, 282
Alw26I GTCTC 1 cut(s) 208
AlwI GGATC 1 cut(s) 191
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 3 cut(s) 17, 20, 282
AspS9I GGNCC 1 cut(s) 251
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 178
BbvI GCAGC 3 cut(s) 4, 32, 269
BccI CCATC 2 cut(s) 155, 161
BcoDI GTCTC 1 cut(s) 208
BfaI CTAG 1 cut(s) 279
BfmI CTRYAG 2 cut(s) 18, 156
BisI GCNGC 3 cut(s) 18, 21, 283
BlsI GCNGC 3 cut(s) 19, 22, 284
Bme18I GGWCC 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 251
BmiI GGNNCC 1 cut(s) 252
BmsI GCATC 2 cut(s) 13, 61
BpiI GAAGAC 1 cut(s) 178
BsaBI GATNNNNATC 1 cut(s) 75
BsaWI WCCGGW 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 200
Bse8I GATNNNNATC 1 cut(s) 75
BseGI GGATG 3 cut(s) 4, 53, 172
BseJI GATNNNNATC 1 cut(s) 75
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 200
BseXI GCAGC 3 cut(s) 4, 32, 269
BshFI GGCC 1 cut(s) 114
BsiSI CCGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 208
BsnI GGCC 1 cut(s) 114
Bsp143I GATC 2 cut(s) 135, 196
BspANI GGCC 1 cut(s) 114
BspCNI CTCAG 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 252
BspMAI CTGCAG 1 cut(s) 22
BspPI GGATC 1 cut(s) 191
BsrI ACTGG 1 cut(s) 200
BssMI GATC 2 cut(s) 135, 196
BstAPI GCANNNNNTGC 1 cut(s) 228
BstDEI CTNAG 1 cut(s) 176
BstF5I GGATG 3 cut(s) 4, 53, 172
BstKTI GATC 2 cut(s) 138, 199
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 2 cut(s) 135, 196
BstMWI GCNNNNNNNGC 1 cut(s) 228
BstSFI CTRYAG 2 cut(s) 18, 156
BstV1I GCAGC 3 cut(s) 4, 32, 269
BstV2I GAAGAC 1 cut(s) 178
BsuRI GGCC 1 cut(s) 114
BtsCI GGATG 3 cut(s) 4, 53, 172
Cfr13I GGNCC 1 cut(s) 251
CviAII CATG 3 cut(s) 38, 194, 215
CviJI RGCY 6 cut(s) 17, 23, 35, 114, 275, 282
CviKI_1 RGCY 6 cut(s) 17, 23, 35, 114, 275, 282
DdeI CTNAG 1 cut(s) 176
DpnI GATC 2 cut(s) 137, 198
DpnII GATC 2 cut(s) 135, 196
Eco47I GGWCC 1 cut(s) 251
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 41, 197, 218
FaiI YATR 5 cut(s) 39, 124, 195, 216, 242
FatI CATG 3 cut(s) 37, 193, 214
Fnu4HI GCNGC 3 cut(s) 18, 21, 283
FokI GGATG 2 cut(s) 60, 179
Fsp4HI GCNGC 3 cut(s) 18, 21, 283
FspBI CTAG 1 cut(s) 279
GluI GCNGC 3 cut(s) 18, 21, 283
HaeIII GGCC 1 cut(s) 114
HapII CCGG 1 cut(s) 254
Hin1II CATG 3 cut(s) 41, 197, 218
HinfI GANTC 2 cut(s) 92, 180
HpaII CCGG 1 cut(s) 254
HphI GGTGA 1 cut(s) 268
Hpy188I TCNGA 1 cut(s) 290
Hpy188III TCNNGA 1 cut(s) 45
HpyAV CCTTC 1 cut(s) 243
HpyCH4V TGCA 5 cut(s) 4, 20, 74, 222, 246
HpyF10VI GCNNNNNNNGC 1 cut(s) 228
HpyF3I CTNAG 1 cut(s) 176
Hsp92II CATG 3 cut(s) 41, 197, 218
Kzo9I GATC 2 cut(s) 135, 196
LpnPI CCDG 3 cut(s) 128, 213, 267
Lsp1109I GCAGC 3 cut(s) 4, 32, 269
LweI GCATC 2 cut(s) 13, 61
MaeI CTAG 1 cut(s) 279
MaeIII GTNAC 1 cut(s) 50
MalI GATC 2 cut(s) 137, 198
MboI GATC 2 cut(s) 135, 196
MboII GAAGA 2 cut(s) 183, 201
MluCI AATT 2 cut(s) 63, 266
MlyI GAGTC 1 cut(s) 101
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 27, 56
MspI CCGG 1 cut(s) 254
MwoI GCNNNNNNNGC 1 cut(s) 228
NdeII GATC 2 cut(s) 135, 196
NlaIII CATG 3 cut(s) 41, 197, 218
NlaIV GGNNCC 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 50
NsiI ATGCAT 1 cut(s) 6
PfeI GAWTC 1 cut(s) 180
PkrI GCNGC 3 cut(s) 19, 22, 284
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 1 cut(s) 251
PstI CTGCAG 1 cut(s) 22
SaqAI TTAA 2 cut(s) 27, 56
SatI GCNGC 3 cut(s) 18, 21, 283
Sau3AI GATC 2 cut(s) 135, 196
Sau96I GGNCC 1 cut(s) 251
SchI GAGTC 1 cut(s) 101
SetI ASST 5 cut(s) 19, 25, 37, 277, 284
SfaNI GCATC 2 cut(s) 13, 61
SfcI CTRYAG 2 cut(s) 18, 156
SinI GGWCC 1 cut(s) 251
Sse9I AATT 2 cut(s) 63, 266
SspMI CTAG 1 cut(s) 279
TasI AATT 2 cut(s) 63, 266
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 2 cut(s) 27, 56
Tru9I TTAA 2 cut(s) 27, 56
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 3 cut(s) 17, 20, 282
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 251
XspI CTAG 1 cut(s) 279
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.