RchiOBHm_Chr6g0270901

Protein EXECUTER 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004166
Physical Location & Seq
Reverse (-)
28919357 .. 28922709
3353 bp
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UTR
Exon/CDS
Intron
PRQ24302

Sequence Viewer

Length: 459 bp
ATGCCCGTTTCTCAAGATCCATTAAATGGACTATACATTGGTGCACATGGGCTCTACACCTCAGAAGTTATTCACCTGAGACGTAAGTTTGGACAATGGAAAGAAGACAATGGGACTAAGGAACCCTCAAACCTCGAGTTTTATGAATATGTGGAAGCCTTAAAACTTACTGGGGATCCTTATGTACCAGCTGGCCAGGTGGCATTCCGTGCAAAGATTGGGAAAAGATATCAGCTTCCTCATAAAGGGATCATACCAGAAGAGTTTGGAGTGGTTGCTCGATATAAAGGACAGGGGAGGCTTGCTGAGCCAGGGTTTCGTAATCCTCGATGGGTCGATGGTGAACTTGTTATTCTTGATGGAAAGTACATTAAAGGAGGACCTGTTGTTGGGTTTGTTTATTGGGCCCCAGAGTATCATTTCTTGGTGTTCTTTAATCGACTGAGGCTTCAAGGCTAG

Protein Analysis

152

Amino Acids

17.34

Weight (kDa)

9.25

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_D1_bind PF12014 3 - 150 6.8e-31 Cyclin D1 binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 26
AclWI GGATC 4 cut(s) 11, 170, 183, 257
AcoI YGGCCR 1 cut(s) 193
AfaI GTAC 2 cut(s) 186, 368
AfiI CCNNNNNNNGG 3 cut(s) 26, 245, 389
AgsI TTSAA 1 cut(s) 452
AjnI CCWGG 2 cut(s) 195, 310
AluBI AGCT 2 cut(s) 191, 235
AluI AGCT 2 cut(s) 191, 235
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 73
Alw44I GTGCAC 1 cut(s) 42
AlwI GGATC 4 cut(s) 11, 170, 183, 257
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 2 cut(s) 193, 405
ApaI GGGCCC 1 cut(s) 409
ApaLI GTGCAC 1 cut(s) 42
Asp700I GAANNNNTTC 1 cut(s) 69
AspS9I GGNCC 3 cut(s) 380, 405, 406
AsuHPI GGTGA 2 cut(s) 65, 353
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 380
BaeGI GKGCMC 2 cut(s) 46, 409
BalI TGGCCA 1 cut(s) 195
BamHI GGATCC 1 cut(s) 175
BanII GRGCYC 2 cut(s) 54, 409
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 46
BccI CCATC 3 cut(s) 324, 332, 353
BciT130I CCWGG 2 cut(s) 197, 312
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 1 cut(s) 457
BlpI GCTNAGC 1 cut(s) 306
Bme1390I CCNGG 2 cut(s) 197, 312
Bme18I GGWCC 1 cut(s) 380
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 3 cut(s) 380, 405, 406
BmiI GGNNCC 4 cut(s) 123, 177, 407, 408
BmrFI CCNGG 2 cut(s) 197, 312
BmrI ACTGGG 1 cut(s) 180
BmuI ACTGGG 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 111
Bpu1102I GCTNAGC 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 311
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 245, 389
Bse1I ACTGG 1 cut(s) 175
BseBI CCWGG 2 cut(s) 197, 312
BseDI CCNNGG 1 cut(s) 311
BseLI CCNNNNNNNGG 3 cut(s) 26, 245, 389
BseMII CTCAG 4 cut(s) 68, 75, 297, 434
BseNI ACTGG 1 cut(s) 175
BseSI GKGCMC 2 cut(s) 46, 409
BshFI GGCC 2 cut(s) 195, 407
BsiHKAI GWGCWC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 3 cut(s) 26, 245, 389
BsmAI GTCTC 1 cut(s) 73
BsmBI CGTCTC 1 cut(s) 73
BsmFI GGGAC 1 cut(s) 127
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 2 cut(s) 195, 407
BsoBI CYCGRG 1 cut(s) 134
Bsp120I GGGCCC 1 cut(s) 405
Bsp1286I GDGCHC 3 cut(s) 46, 54, 409
Bsp143I GATC 3 cut(s) 16, 175, 249
Bsp1720I GCTNAGC 1 cut(s) 306
BspANI GGCC 2 cut(s) 195, 407
BspCNI CTCAG 4 cut(s) 69, 74, 298, 435
BspLI GGNNCC 4 cut(s) 123, 177, 407, 408
BspPI GGATC 4 cut(s) 11, 170, 183, 257
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 311
BssMI GATC 3 cut(s) 16, 175, 249
Bst2UI CCWGG 2 cut(s) 197, 312
Bst6I CTCTTC 1 cut(s) 255
BstAPI GCANNNNNTGC 1 cut(s) 209
BstC8I GCNNGC 2 cut(s) 193, 303
BstDEI CTNAG 5 cut(s) 61, 77, 117, 306, 443
BstENI CCTNNNNNAGG 1 cut(s) 243
BstKTI GATC 3 cut(s) 19, 178, 252
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 3 cut(s) 16, 175, 249
BstMWI GCNNNNNNNGC 2 cut(s) 209, 307
BstNI CCWGG 2 cut(s) 197, 312
BstSCI CCNGG 2 cut(s) 195, 310
BstSLI GKGCMC 2 cut(s) 46, 409
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 2 cut(s) 16, 175
BstYI RGATCY 2 cut(s) 16, 175
BsuRI GGCC 2 cut(s) 195, 407
Cac8I GCNNGC 2 cut(s) 193, 303
Cfr13I GGNCC 3 cut(s) 380, 405, 406
Csp6I GTAC 2 cut(s) 185, 367
CviAII CATG 1 cut(s) 47
CviQI GTAC 2 cut(s) 185, 367
DdeI CTNAG 5 cut(s) 61, 77, 117, 306, 443
DpnI GATC 3 cut(s) 18, 177, 251
DpnII GATC 3 cut(s) 16, 175, 249
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco24I GRGCYC 2 cut(s) 54, 409
Eco32I GATATC 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 380
Eco88I CYCGRG 1 cut(s) 134
EcoNI CCTNNNNNAGG 1 cut(s) 243
EcoO109I RGGNCCY 2 cut(s) 380, 406
EcoRII CCWGG 2 cut(s) 195, 310
EcoRV GATATC 1 cut(s) 230
EcoT38I GRGCYC 2 cut(s) 54, 409
Esp3I CGTCTC 1 cut(s) 73
FaeI CATG 1 cut(s) 50
FaiI YATR 8 cut(s) 34, 48, 144, 150, 183, 243, 254, 285
FaqI GGGAC 1 cut(s) 127
FatI CATG 1 cut(s) 46
FriOI GRGCYC 2 cut(s) 54, 409
FspBI CTAG 1 cut(s) 457
HaeIII GGCC 2 cut(s) 195, 407
Hin1II CATG 1 cut(s) 50
HphI GGTGA 2 cut(s) 65, 353
Hpy166II GTNNAC 2 cut(s) 44, 344
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 2 cut(s) 14, 356
Hpy8I GTNNAC 2 cut(s) 44, 344
HpyCH4IV ACGT 1 cut(s) 82
HpyCH4V TGCA 2 cut(s) 44, 212
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 307
HpyF3I CTNAG 5 cut(s) 61, 77, 117, 306, 443
HpySE526I ACGT 1 cut(s) 82
Hsp92II CATG 1 cut(s) 50
Kzo9I GATC 3 cut(s) 16, 175, 249
MaeI CTAG 1 cut(s) 457
MaeII ACGT 1 cut(s) 82
MalI GATC 3 cut(s) 18, 177, 251
MboI GATC 3 cut(s) 16, 175, 249
MboII GAAGA 2 cut(s) 116, 272
MflI RGATCY 2 cut(s) 16, 175
MhlI GDGCHC 3 cut(s) 46, 54, 409
MlsI TGGCCA 1 cut(s) 195
MluNI TGGCCA 1 cut(s) 195
MnlI CCTC 8 cut(s) 70, 136, 143, 249, 291, 336, 371, 438
Mox20I TGGCCA 1 cut(s) 195
MroXI GAANNNNTTC 1 cut(s) 69
MscI TGGCCA 1 cut(s) 195
MseI TTAA 4 cut(s) 23, 161, 372, 435
Msp20I TGGCCA 1 cut(s) 195
MspA1I CMGCKG 1 cut(s) 191
MspR9I CCNGG 2 cut(s) 197, 312
Mva1269I GAATGC 1 cut(s) 203
MvaI CCWGG 2 cut(s) 197, 312
MwoI GCNNNNNNNGC 2 cut(s) 209, 307
NdeII GATC 3 cut(s) 16, 175, 249
NlaIII CATG 1 cut(s) 50
NlaIV GGNNCC 4 cut(s) 123, 177, 407, 408
PaeR7I CTCGAG 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 325
PctI GAATGC 1 cut(s) 203
PdmI GAANNNNTTC 1 cut(s) 69
PflMI CCANNNNNTGG 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 2 cut(s) 195, 310
PspGI CCWGG 2 cut(s) 195, 310
PspN4I GGNNCC 4 cut(s) 123, 177, 407, 408
PspOMI GGGCCC 1 cut(s) 405
PspPI GGNCC 3 cut(s) 380, 405, 406
PspPPI RGGWCCY 1 cut(s) 380
PspXI VCTCGAGB 1 cut(s) 134
PsuI RGATCY 2 cut(s) 16, 175
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 2 cut(s) 186, 368
RsaNI GTAC 2 cut(s) 185, 367
SaqAI TTAA 4 cut(s) 23, 161, 372, 435
Sau3AI GATC 3 cut(s) 16, 175, 249
Sau96I GGNCC 3 cut(s) 380, 405, 406
ScrFI CCNGG 2 cut(s) 197, 312
SduI GDGCHC 3 cut(s) 46, 54, 409
SetI ASST 8 cut(s) 62, 78, 85, 135, 193, 201, 237, 385
Sfr274I CTCGAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 380
SlaI CTCGAG 1 cut(s) 134
SmlI CTYRAG 2 cut(s) 12, 134
SmoI CTYRAG 2 cut(s) 12, 134
SspMI CTAG 1 cut(s) 457
StyD4I CCNGG 2 cut(s) 195, 310
TaiI ACGT 1 cut(s) 85
TaqI TCGA 5 cut(s) 135, 280, 328, 336, 439
TatI WGTACW 1 cut(s) 366
Tru1I TTAA 4 cut(s) 23, 161, 372, 435
Tru9I TTAA 4 cut(s) 23, 161, 372, 435
TspDTI ATGAA 1 cut(s) 159
TspGWI ACGGA 1 cut(s) 197
Van91I CCANNNNNTGG 1 cut(s) 26
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 380
XagI CCTNNNNNAGG 1 cut(s) 243
XhoI CTCGAG 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.