Rroxscaffold_7G00197500

Protein EXECUTER 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004166
Physical Location & Seq
Forward (+)
41851869 .. 41859332
7464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00197500.1

Sequence Viewer

Length: 1020 bp
ATGGCTTCCATCTCTGCTCCAACCCCAGCTCCTCACAACCTCACCTTCCCCAACCCCAAATCCTACCCCTCCTCCGCTTCGAAAACCCCATTGTACGCTTCCTCTTCCTCTAGATTCTCCGATTCATCCCTCTGCCGGTGCCGGAACCCTTCCTCCTCCTCCGACGACTCAGTCCAGTGGAGATGGGACTCTGCGCTCCAGGACCTCCTCAAAAACGCCATGAAACGACTCGATTTCCAAGGAGGAGGAGGAGGAGGAGGCGCTACTGCTAGCAAGGCCGACGAGGAGGAGCCTGACTGGGATTGGGATCGCTGGCGTCAGCATTTCCTCGAGGTCGAAGACCAAGAGCGCCTCGTCTCACTTCTCAAGTCACAGTTGGGTCGAGCTGTAAATGGTGAGGATTTTGAGGATGCTGCTAGGCTCAAGGTCGCCATTGCTGCCGCCTCCACAAACGACGTCGTCGGCTCAGTCATTTCTCGTCTCAATGTCTCTCATATCATCTTTCCACTTAGAGCTATACAGGACGAGCGTTACCAGGAAGCAGCATTTTTCCGAGATAATGCCGGTGCTGGATTGCTTTGGCCAGTTAGACTATACATTGGTGCACATGGGCTCTACACCTCAGAAGTTATTCACCTGAGACGGAAGTTTGGCCAATGGAAAGAAGACAATGGGACTAAGGAACCCTCAAACCTCGAGTTTTATGAATATGTGGAAGCCTTAAAACTTACTGGGGATCCTTATGTACCAGCTGGCCAGGTGGCATTCCGTGCAAAGATTGGGAAAAGATATCAGCTTCCTCATAAGGGGATCATACCAGAAGAGTTTGGAGTGGTTGCTCGATATAAAGGACAGGGGAGGCTTGCTGAGCCAGGGTTTCGTAATCCTCGATGGGTCGATGGTGAACTTGTTATTCTTGATGGAAAGTACATTAAAGGAGGACCTGTTGTTGGGTTTGTTTATTGGGCCCCAGAGTATCATTTCTTGGTGTTCTTTAATCGACTGAGGCTTCAAGGCTAG

Protein Analysis

339

Amino Acids

37.96

Weight (kDa)

7.76

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_D1_bind PF12014 197 - 337 7.9e-27 Cyclin D1 binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
AatII GACGTC 1 cut(s) 459
AbsI CCTCGAGG 1 cut(s) 329
AccB1I GGYRCC 1 cut(s) 138
AciI CCGC 2 cut(s) 75, 441
AclWI GGATC 4 cut(s) 315, 731, 744, 818
AcoI YGGCCR 3 cut(s) 581, 652, 754
AcyI GRCGYC 2 cut(s) 316, 456
AfaI GTAC 3 cut(s) 95, 747, 929
AfiI CCNNNNNNNGG 3 cut(s) 135, 806, 950
AgsI TTSAA 1 cut(s) 1013
AjnI CCWGG 4 cut(s) 198, 534, 756, 871
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
AluBI AGCT 5 cut(s) 29, 386, 515, 752, 796
AluI AGCT 5 cut(s) 29, 386, 515, 752, 796
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 4 cut(s) 361, 485, 493, 634
Alw44I GTGCAC 1 cut(s) 603
AlwI GGATC 4 cut(s) 315, 731, 744, 818
Ama87I CYCGRG 2 cut(s) 329, 695
AoxI GGCC 5 cut(s) 276, 581, 652, 754, 966
ApaI GGGCCC 1 cut(s) 970
ApaLI GTGCAC 1 cut(s) 603
ApeKI GCWGC 3 cut(s) 413, 437, 542
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 3 cut(s) 196, 263, 351
AspS9I GGNCC 4 cut(s) 202, 941, 966, 967
AsuHPI GGTGA 4 cut(s) 34, 407, 626, 914
AsuII TTCGAA 1 cut(s) 80
AsuNHI GCTAGC 1 cut(s) 269
AvaI CYCGRG 2 cut(s) 329, 695
AvaII GGWCC 2 cut(s) 202, 941
BaeGI GKGCMC 2 cut(s) 607, 970
BalI TGGCCA 3 cut(s) 583, 654, 756
BamHI GGATCC 1 cut(s) 736
BanI GGYRCC 1 cut(s) 138
BanII GRGCYC 2 cut(s) 615, 970
BbsI GAAGAC 2 cut(s) 345, 672
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 3 cut(s) 400, 424, 554
BccI CCATC 5 cut(s) 17, 177, 885, 893, 914
BciT130I CCWGG 4 cut(s) 200, 536, 758, 873
BcoDI GTCTC 4 cut(s) 361, 485, 493, 634
BfaI CTAG 4 cut(s) 111, 270, 417, 1018
BfoI RGCGCY 2 cut(s) 264, 352
BisI GCNGC 4 cut(s) 414, 438, 441, 543
BlpI GCTNAGC 1 cut(s) 867
BlsI GCNGC 4 cut(s) 415, 439, 442, 544
Bme1390I CCNGG 4 cut(s) 200, 536, 758, 873
Bme18I GGWCC 2 cut(s) 202, 941
BmeT110I CYCGRG 2 cut(s) 329, 695
BmgT120I GGNCC 4 cut(s) 202, 941, 966, 967
BmiI GGNNCC 7 cut(s) 140, 146, 291, 684, 738, 968, 969
BmrFI CCNGG 4 cut(s) 200, 536, 758, 873
BmrI ACTGGG 2 cut(s) 307, 741
BmsI GCATC 1 cut(s) 400
BmtI GCTAGC 1 cut(s) 273
BmuI ACTGGG 2 cut(s) 307, 741
BpiI GAAGAC 2 cut(s) 345, 672
BpmI CTGGAG 1 cut(s) 182
Bpu1102I GCTNAGC 1 cut(s) 867
Bpu14I TTCGAA 1 cut(s) 80
BpuEI CTTGAG 2 cut(s) 350, 407
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 2 cut(s) 316, 456
BsaJI CCNNGG 2 cut(s) 238, 872
BsaXI ACNNNNNCTCC 8 cut(s) 13, 43, 56, 86, 137, 167, 930, 960
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 806, 950
Bse118I RCCGGY 2 cut(s) 135, 563
Bse1I ACTGG 4 cut(s) 175, 302, 584, 736
Bse3DI GCAATG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 4 cut(s) 200, 536, 758, 873
BseDI CCNNGG 2 cut(s) 238, 872
BseGI GGATG 2 cut(s) 125, 415
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 3 cut(s) 135, 806, 950
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 6 cut(s) 183, 480, 629, 636, 858, 995
BseNI ACTGG 4 cut(s) 175, 302, 584, 736
BseSI GKGCMC 2 cut(s) 607, 970
BseXI GCAGC 3 cut(s) 400, 424, 554
BseYI CCCAGC 1 cut(s) 25
BshFI GGCC 5 cut(s) 278, 583, 654, 756, 968
BshNI GGYRCC 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 607
BsiHKCI CYCGRG 2 cut(s) 329, 695
BsiSI CCGG 3 cut(s) 136, 142, 564
BslFI GGGAC 2 cut(s) 200, 688
BslI CCNNNNNNNGG 3 cut(s) 135, 806, 950
BsmAI GTCTC 4 cut(s) 361, 485, 493, 634
BsmBI CGTCTC 3 cut(s) 361, 485, 634
BsmFI GGGAC 2 cut(s) 200, 688
BsmI GAATGC 1 cut(s) 764
BsnI GGCC 5 cut(s) 278, 583, 654, 756, 968
BsoBI CYCGRG 2 cut(s) 329, 695
Bsp119I TTCGAA 1 cut(s) 80
Bsp120I GGGCCC 1 cut(s) 966
Bsp1286I GDGCHC 3 cut(s) 607, 615, 970
Bsp143I GATC 3 cut(s) 307, 736, 810
Bsp1720I GCTNAGC 1 cut(s) 867
BspACI CCGC 2 cut(s) 75, 441
BspANI GGCC 5 cut(s) 278, 583, 654, 756, 968
BspCNI CTCAG 6 cut(s) 182, 479, 630, 635, 859, 996
BspLI GGNNCC 7 cut(s) 140, 146, 291, 684, 738, 968, 969
BspOI GCTAGC 1 cut(s) 273
BspPI GGATC 4 cut(s) 315, 731, 744, 818
BspT104I TTCGAA 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 2 cut(s) 135, 563
BsrI ACTGG 4 cut(s) 175, 302, 584, 736
BssAI RCCGGY 2 cut(s) 135, 563
BssECI CCNNGG 2 cut(s) 238, 872
BssMI GATC 3 cut(s) 307, 736, 810
BssNI GRCGYC 2 cut(s) 316, 456
BssT1I CCWWGG 1 cut(s) 238
Bst2UI CCWGG 4 cut(s) 200, 536, 758, 873
Bst4CI ACNGT 1 cut(s) 375
Bst6I CTCTTC 2 cut(s) 109, 816
BstACI GRCGYC 2 cut(s) 316, 456
BstAPI GCANNNNNTGC 1 cut(s) 770
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 4 cut(s) 271, 314, 754, 864
BstDEI CTNAG 8 cut(s) 169, 466, 509, 622, 638, 678, 867, 1004
BstF5I GGATG 2 cut(s) 125, 415
BstH2I RGCGCY 2 cut(s) 264, 352
BstHHI GCGC 3 cut(s) 196, 263, 351
BstKTI GATC 3 cut(s) 310, 739, 813
BstMAI GTCTC 4 cut(s) 361, 485, 493, 634
BstMBI GATC 3 cut(s) 307, 736, 810
BstMWI GCNNNNNNNGC 4 cut(s) 275, 437, 770, 868
BstNI CCWGG 4 cut(s) 200, 536, 758, 873
BstSCI CCNGG 4 cut(s) 198, 534, 756, 871
BstSLI GKGCMC 2 cut(s) 607, 970
BstV1I GCAGC 3 cut(s) 400, 424, 554
BstV2I GAAGAC 2 cut(s) 345, 672
BstX2I RGATCY 1 cut(s) 736
BstYI RGATCY 1 cut(s) 736
BsuRI GGCC 5 cut(s) 278, 583, 654, 756, 968
BtsCI GGATG 2 cut(s) 125, 415
BtsIMutI CAGTG 1 cut(s) 182
Cac8I GCNNGC 4 cut(s) 271, 314, 754, 864
CfoI GCGC 3 cut(s) 196, 263, 351
Cfr10I RCCGGY 2 cut(s) 135, 563
Cfr13I GGNCC 4 cut(s) 202, 941, 966, 967
CseI GACGC 1 cut(s) 305
Csp6I GTAC 3 cut(s) 94, 746, 928
CviAII CATG 2 cut(s) 220, 608
CviQI GTAC 3 cut(s) 94, 746, 928
DdeI CTNAG 8 cut(s) 169, 466, 509, 622, 638, 678, 867, 1004
DpnI GATC 3 cut(s) 309, 738, 812
DpnII GATC 3 cut(s) 307, 736, 810
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
EaeI YGGCCR 3 cut(s) 581, 652, 754
Eam1104I CTCTTC 2 cut(s) 109, 816
EarI CTCTTC 2 cut(s) 109, 816
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 2 cut(s) 615, 970
Eco32I GATATC 1 cut(s) 791
Eco47I GGWCC 2 cut(s) 202, 941
Eco88I CYCGRG 2 cut(s) 329, 695
EcoO109I RGGNCCY 3 cut(s) 202, 941, 967
EcoRII CCWGG 4 cut(s) 198, 534, 756, 871
EcoRV GATATC 1 cut(s) 791
EcoT14I CCWWGG 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 615, 970
ErhI CCWWGG 1 cut(s) 238
Esp3I CGTCTC 3 cut(s) 361, 485, 634
FaeI CATG 2 cut(s) 223, 611
FaqI GGGAC 2 cut(s) 200, 688
FatI CATG 2 cut(s) 219, 607
Fnu4HI GCNGC 4 cut(s) 414, 438, 441, 543
FokI GGATG 2 cut(s) 112, 422
FriOI GRGCYC 2 cut(s) 615, 970
Fsp4HI GCNGC 4 cut(s) 414, 438, 441, 543
FspBI CTAG 4 cut(s) 111, 270, 417, 1018
GlaI GCGC 3 cut(s) 195, 262, 350
GluI GCNGC 4 cut(s) 414, 438, 441, 543
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 1 cut(s) 182
HaeII RGCGCY 2 cut(s) 264, 352
HaeIII GGCC 5 cut(s) 278, 583, 654, 756, 968
HapII CCGG 3 cut(s) 136, 142, 564
HgaI GACGC 1 cut(s) 305
HhaI GCGC 3 cut(s) 196, 263, 351
Hin1I GRCGYC 2 cut(s) 316, 456
Hin1II CATG 2 cut(s) 223, 611
Hin6I GCGC 3 cut(s) 194, 261, 349
HinP1I GCGC 3 cut(s) 194, 261, 349
HinfI GANTC 5 cut(s) 114, 122, 167, 188, 228
HpaII CCGG 3 cut(s) 136, 142, 564
HphI GGTGA 4 cut(s) 34, 407, 626, 914
Hpy166II GTNNAC 2 cut(s) 605, 905
Hpy188I TCNGA 4 cut(s) 121, 163, 554, 625
Hpy188III TCNNGA 2 cut(s) 111, 917
Hpy8I GTNNAC 2 cut(s) 605, 905
Hpy99I CGWCG 5 cut(s) 167, 284, 458, 461, 464
HpyAV CCTTC 2 cut(s) 55, 159
HpyCH4III ACNGT 1 cut(s) 375
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 2 cut(s) 605, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 437, 770, 868
HpyF3I CTNAG 8 cut(s) 169, 466, 509, 622, 638, 678, 867, 1004
HpySE526I ACGT 1 cut(s) 456
Hsp92I GRCGYC 2 cut(s) 316, 456
Hsp92II CATG 2 cut(s) 223, 611
HspAI GCGC 3 cut(s) 194, 261, 349
Kzo9I GATC 3 cut(s) 307, 736, 810
LmnI GCTCC 4 cut(s) 22, 34, 201, 289
Lsp1109I GCAGC 3 cut(s) 400, 424, 554
LweI GCATC 1 cut(s) 400
MaeI CTAG 4 cut(s) 111, 270, 417, 1018
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 2 cut(s) 369, 530
MalI GATC 3 cut(s) 309, 738, 812
MboI GATC 3 cut(s) 307, 736, 810
MboII GAAGA 4 cut(s) 96, 350, 677, 833
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 3 cut(s) 607, 615, 970
MlsI TGGCCA 3 cut(s) 583, 654, 756
MluNI TGGCCA 3 cut(s) 583, 654, 756
MlyI GAGTC 3 cut(s) 161, 182, 222
MmeI TCCRAC 2 cut(s) 44, 186
Mox20I TGGCCA 3 cut(s) 583, 654, 756
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 3 cut(s) 583, 654, 756
MseI TTAA 3 cut(s) 722, 933, 996
Msp20I TGGCCA 3 cut(s) 583, 654, 756
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 3 cut(s) 136, 142, 564
MspR9I CCNGG 4 cut(s) 200, 536, 758, 873
Mva1269I GAATGC 1 cut(s) 764
MvaI CCWGG 4 cut(s) 200, 536, 758, 873
MwoI GCNNNNNNNGC 4 cut(s) 275, 437, 770, 868
NdeII GATC 3 cut(s) 307, 736, 810
NheI GCTAGC 1 cut(s) 269
NlaIII CATG 2 cut(s) 223, 611
NlaIV GGNNCC 7 cut(s) 140, 146, 291, 684, 738, 968, 969
NmuCI GTSAC 1 cut(s) 369
NspV TTCGAA 1 cut(s) 80
PaeR7I CTCGAG 2 cut(s) 329, 695
PcsI WCGNNNNNNNCGW 1 cut(s) 886
PctI GAATGC 1 cut(s) 764
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 114, 122
PflFI GACNNNGTC 2 cut(s) 170, 458
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 4 cut(s) 415, 439, 442, 544
PleI GAGTC 3 cut(s) 161, 182, 222
PpsI GAGTC 3 cut(s) 161, 182, 222
PpuMI RGGWCCY 2 cut(s) 202, 941
Psp5II RGGWCCY 2 cut(s) 202, 941
Psp6I CCWGG 4 cut(s) 198, 534, 756, 871
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 4 cut(s) 198, 534, 756, 871
PspN4I GGNNCC 7 cut(s) 140, 146, 291, 684, 738, 968, 969
PspOMI GGGCCC 1 cut(s) 966
PspPI GGNCC 4 cut(s) 202, 941, 966, 967
PspPPI RGGWCCY 2 cut(s) 202, 941
PspXI VCTCGAGB 2 cut(s) 329, 695
PsuI RGATCY 1 cut(s) 736
PsyI GACNNNGTC 2 cut(s) 170, 458
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 3 cut(s) 95, 747, 929
RsaNI GTAC 3 cut(s) 94, 746, 928
SaqAI TTAA 3 cut(s) 722, 933, 996
SatI GCNGC 4 cut(s) 414, 438, 441, 543
Sau3AI GATC 3 cut(s) 307, 736, 810
Sau96I GGNCC 4 cut(s) 202, 941, 966, 967
SchI GAGTC 3 cut(s) 161, 182, 222
ScrFI CCNGG 4 cut(s) 200, 536, 758, 873
SduI GDGCHC 3 cut(s) 607, 615, 970
SfaNI GCATC 1 cut(s) 400
Sfr274I CTCGAG 2 cut(s) 329, 695
SfuI TTCGAA 1 cut(s) 80
SinI GGWCC 2 cut(s) 202, 941
SlaI CTCGAG 2 cut(s) 329, 695
SmlI CTYRAG 4 cut(s) 329, 365, 422, 695
SmoI CTYRAG 4 cut(s) 329, 365, 422, 695
SsiI CCGC 2 cut(s) 75, 441
SspMI CTAG 4 cut(s) 111, 270, 417, 1018
StyD4I CCNGG 4 cut(s) 198, 534, 756, 871
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 1 cut(s) 375
TaiI ACGT 1 cut(s) 459
TatI WGTACW 1 cut(s) 927
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 2 cut(s) 114, 122
Tru1I TTAA 3 cut(s) 722, 933, 996
Tru9I TTAA 3 cut(s) 722, 933, 996
TscAI CASTG 1 cut(s) 182
TseFI GTSAC 1 cut(s) 369
TseI GCWGC 3 cut(s) 413, 437, 542
Tsp45I GTSAC 1 cut(s) 369
TspDTI ATGAA 3 cut(s) 114, 236, 720
TspGWI ACGGA 2 cut(s) 658, 758
TspRI CASTG 1 cut(s) 182
Tth111I GACNNNGTC 2 cut(s) 170, 458
VneI GTGCAC 1 cut(s) 603
VpaK11BI GGWCC 2 cut(s) 202, 941
XbaI TCTAGA 1 cut(s) 110
XhoI CTCGAG 2 cut(s) 329, 695
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 4 cut(s) 111, 270, 417, 1018
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.