RchiOBHm_Chr6g0280621

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0019028
Physical Location & Seq
Reverse (-)
44010601 .. 44013434
2834 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25164

Sequence Viewer

Length: 1035 bp
ATGCCGTATAGTAAAGAACCTGCTAATAAGGTAATAACAACTTTCATGTCACCTGCACTGGCCTCAACCTACCTGGCATTTCATCCTTTGAACCACAATATCATAACGATTGTAACAGAAAATTCAATCATTCACATTTACTATCTTAGAGTTGATGAGGTGATCAGACCAAAATTCAAGTGCCATCAGAAACACATTCCTGGATTTGCATTTTCTGTCAACCTTAAAGTCATGGTTTCATCAGAGGCTGATGCTCAAGTTCAAGTACATGAGATCTGTTCTTTTCTTAAATCATTAGGTCTTGCTGCACGTAGTACATGCTGGGTTCTTTTGAGAATGATTGTTGGTGAGATTATCTGCAGATGGTCTATAGATGCTTGGACATATTCTATGACCTACAACTTGATTGAACTACGCTTTTGGAACATGGATACCTGGGATAAGAGGAAATTAGTTAGGATTCAACTGCCAACTGGAAAGGCGCCTACTGGTGATACTCAAGTGCAATTCTACTCTGATCAAATCCTGTTGTTGGTATTGGTTAAGACTCAACCAGCACTATATGATGGTGCCAAAACAGAATGCATTCAGCAGTGGATGCCACGAGATGTGCTGCCTGCACCGATAACATGTGCAGCATATTCCCCGAATAATCAACTCTATGGGGCTTTATCTGATAGCAATGTTGCAGTATTTGATACTGACAGTCTGAAACTTAGATGTCGTATAGCCATGTCAGTGTACTTTGTAGAAGCATCCCGCAACAGGTTCAAGGTTCCTCAACCTGGTTCAAACGCTGGGATTGGTGCTGTGCTAGTGACTGCTCAGGAAGTGGAAGCTCATAGTGAAAAGGGTCTGCTTGTTGGAGCGGTTGCAGAGAGTGATGCTACTGAGGACCCCATTTTTCCTGGAAAGCGCATGAGCAAGATGAAGGACGCAGCTGGGGTATCTCTCAGAGTATTTTTGGCGACACCAGGGGGAAGATTTTTACTGTTGCTGAGACTGAGCTCAACAAAACTGCTGCTGCTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

344

Amino Acids

38.39

Weight (kDa)

8.93

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019028)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0280621
rosa_laevigata RLG00000013043
rosa_roxburghii Rroxscaffold_7G00187870
rosa_rugosa Rorug06G0132900
rosa_samantha Rh6AG245500 Rh6BG247800 Rh6CG249300 Rh6DG240500
rosa_wichuraiana Rw6G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 61
Acc36I ACCTGC 2 cut(s) 28, 61
AccB1I GGYRCC 2 cut(s) 481, 569
AccBSI CCGCTC 1 cut(s) 869
AciI CCGC 2 cut(s) 760, 869
AcsI RAATTY 2 cut(s) 121, 173
AcyI GRCGYC 1 cut(s) 482
AfaI GTAC 3 cut(s) 267, 316, 743
AfiI CCNNNNNNNGG 3 cut(s) 532, 765, 785
AflIII ACRYGT 1 cut(s) 629
AgsI TTSAA 8 cut(s) 91, 126, 178, 263, 410, 464, 772, 792
AjnI CCWGG 6 cut(s) 72, 199, 434, 784, 907, 973
AluBI AGCT 3 cut(s) 839, 941, 1008
AluI AGCT 3 cut(s) 839, 941, 1008
Alw21I GWGCWC 1 cut(s) 1010
Alw26I GTCTC 1 cut(s) 994
AlwNI CAGNNNCTG 1 cut(s) 248
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 6 cut(s) 305, 613, 635, 938, 1021, 1024
ApoI RAATTY 2 cut(s) 121, 173
Asp700I GAANNNNTTC 1 cut(s) 585
AspLEI GCGC 2 cut(s) 484, 918
AspS9I GGNCC 1 cut(s) 895
AsuHPI GGTGA 4 cut(s) 42, 172, 359, 503
AvaII GGWCC 1 cut(s) 895
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BanI GGYRCC 2 cut(s) 481, 569
BanII GRGCYC 1 cut(s) 1010
BauI CACGAG 1 cut(s) 603
Bbv12I GWGCWC 1 cut(s) 1010
BbvI GCAGC 6 cut(s) 292, 600, 647, 950, 1008, 1011
BccI CCATC 3 cut(s) 192, 357, 560
BciT130I CCWGG 6 cut(s) 74, 201, 436, 786, 909, 975
BciVI GTATCC 1 cut(s) 424
BclI TGATCA 2 cut(s) 162, 517
BcoDI GTCTC 1 cut(s) 994
BfaI CTAG 1 cut(s) 815
BfmI CTRYAG 2 cut(s) 358, 369
BfoI RGCGCY 1 cut(s) 485
BfuAI ACCTGC 2 cut(s) 28, 61
BfuI GTATCC 1 cut(s) 424
BglII AGATCT 1 cut(s) 273
BisI GCNGC 6 cut(s) 306, 614, 636, 939, 1022, 1025
BlsI GCNGC 6 cut(s) 307, 615, 637, 940, 1023, 1026
Bme1390I CCNGG 6 cut(s) 74, 201, 436, 786, 909, 975
Bme18I GGWCC 1 cut(s) 895
BmgT120I GGNCC 1 cut(s) 895
BmiI GGNNCC 4 cut(s) 483, 571, 777, 897
BmrFI CCNGG 6 cut(s) 74, 201, 436, 786, 909, 975
BmsI GCATC 5 cut(s) 241, 364, 588, 764, 874
Bpu10I CCTNAGC 1 cut(s) 825
BpuEI CTTGAG 2 cut(s) 240, 483
BsaAI YACGTR 1 cut(s) 311
BsaHI GRCGYC 1 cut(s) 482
BsaJI CCNNGG 2 cut(s) 435, 974
Bsc4I CCNNNNNNNGG 3 cut(s) 532, 765, 785
Bse1I ACTGG 3 cut(s) 63, 478, 493
Bse3DI GCAATG 1 cut(s) 688
BseBI CCWGG 6 cut(s) 74, 201, 436, 786, 909, 975
BseDI CCNNGG 2 cut(s) 435, 974
BseGI GGATG 3 cut(s) 82, 603, 755
BseLI CCNNNNNNNGG 3 cut(s) 532, 765, 785
BseMI GCAATG 1 cut(s) 688
BseMII CTCAG 5 cut(s) 839, 882, 967, 989, 995
BseNI ACTGG 3 cut(s) 63, 478, 493
BseXI GCAGC 6 cut(s) 292, 600, 647, 950, 1008, 1011
BseYI CCCAGC 3 cut(s) 321, 797, 941
BsgI GTGCAG 4 cut(s) 39, 291, 603, 654
BshFI GGCC 1 cut(s) 62
BshNI GGYRCC 2 cut(s) 481, 569
BsiHKAI GWGCWC 1 cut(s) 1010
BslI CCNNNNNNNGG 3 cut(s) 532, 765, 785
BsmAI GTCTC 1 cut(s) 994
BsmI GAATGC 2 cut(s) 585, 587
BsnI GGCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 1010
Bsp143I GATC 3 cut(s) 162, 273, 517
BspACI CCGC 2 cut(s) 760, 869
BspANI GGCC 1 cut(s) 62
BspCNI CTCAG 5 cut(s) 838, 883, 966, 990, 996
BspLI GGNNCC 4 cut(s) 483, 571, 777, 897
BspMAI CTGCAG 1 cut(s) 362
BspMI ACCTGC 2 cut(s) 28, 61
BspT107I GGYRCC 2 cut(s) 481, 569
BsrBI CCGCTC 1 cut(s) 869
BsrDI GCAATG 1 cut(s) 688
BsrI ACTGG 3 cut(s) 63, 478, 493
BssECI CCNNGG 2 cut(s) 435, 974
BssMI GATC 3 cut(s) 162, 273, 517
BssNI GRCGYC 1 cut(s) 482
BssSI CACGAG 1 cut(s) 603
Bst2BI CACGAG 1 cut(s) 603
Bst2UI CCWGG 6 cut(s) 74, 201, 436, 786, 909, 975
Bst4CI ACNGT 2 cut(s) 707, 993
BstACI GRCGYC 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 598
BstBAI YACGTR 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 7 cut(s) 146, 716, 825, 891, 953, 998, 1004
BstF5I GGATG 3 cut(s) 82, 603, 755
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 2 cut(s) 484, 918
BstKTI GATC 3 cut(s) 165, 276, 520
BstMAI GTCTC 1 cut(s) 994
BstMBI GATC 3 cut(s) 162, 273, 517
BstMWI GCNNNNNNNGC 1 cut(s) 598
BstNI CCWGG 6 cut(s) 74, 201, 436, 786, 909, 975
BstNSI RCATGY 2 cut(s) 321, 633
BstSCI CCNGG 6 cut(s) 72, 199, 434, 784, 907, 973
BstSFI CTRYAG 2 cut(s) 358, 369
BstV1I GCAGC 6 cut(s) 292, 600, 647, 950, 1008, 1011
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 3 cut(s) 82, 603, 755
BtsI GCAGTG 1 cut(s) 599
BtsIMutI CAGTG 3 cut(s) 56, 599, 744
BveI ACCTGC 2 cut(s) 28, 61
Cac8I GCNNGC 1 cut(s) 618
CaiI CAGNNNCTG 1 cut(s) 248
CfoI GCGC 2 cut(s) 484, 918
Cfr13I GGNCC 1 cut(s) 895
CseI GACGC 1 cut(s) 944
CsiI ACCWGGT 1 cut(s) 784
Csp6I GTAC 3 cut(s) 266, 315, 742
CviAII CATG 8 cut(s) 46, 232, 269, 318, 427, 630, 733, 919
CviJI RGCY 7 cut(s) 62, 248, 668, 731, 839, 941, 1008
CviKI_1 RGCY 7 cut(s) 62, 248, 668, 731, 839, 941, 1008
CviQI GTAC 3 cut(s) 266, 315, 742
DdeI CTNAG 7 cut(s) 146, 716, 825, 891, 953, 998, 1004
DinI GGCGCC 1 cut(s) 483
DpnI GATC 3 cut(s) 164, 275, 519
DpnII GATC 3 cut(s) 162, 273, 517
Ecl136II GAGCTC 1 cut(s) 1008
Eco24I GRGCYC 1 cut(s) 1010
Eco47I GGWCC 1 cut(s) 895
Eco53kI GAGCTC 1 cut(s) 1008
EcoICRI GAGCTC 1 cut(s) 1008
EcoO109I RGGNCCY 1 cut(s) 895
EcoRII CCWGG 6 cut(s) 72, 199, 434, 784, 907, 973
EcoT22I ATGCAT 1 cut(s) 587
EcoT38I GRGCYC 1 cut(s) 1010
EgeI GGCGCC 1 cut(s) 483
EheI GGCGCC 1 cut(s) 483
FaeI CATG 8 cut(s) 49, 235, 272, 321, 430, 633, 736, 922
FatI CATG 8 cut(s) 45, 231, 268, 317, 426, 629, 732, 918
FauI CCCGC 1 cut(s) 767
FbaI TGATCA 2 cut(s) 162, 517
Fnu4HI GCNGC 6 cut(s) 306, 614, 636, 939, 1022, 1025
FokI GGATG 3 cut(s) 69, 610, 742
FriOI GRGCYC 1 cut(s) 1010
Fsp4HI GCNGC 6 cut(s) 306, 614, 636, 939, 1022, 1025
FspBI CTAG 1 cut(s) 815
GlaI GCGC 2 cut(s) 483, 917
GluI GCNGC 6 cut(s) 306, 614, 636, 939, 1022, 1025
GsaI CCCAGC 3 cut(s) 325, 801, 945
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 1 cut(s) 62
HgaI GACGC 1 cut(s) 944
HhaI GCGC 2 cut(s) 484, 918
Hin1I GRCGYC 1 cut(s) 482
Hin1II CATG 8 cut(s) 49, 235, 272, 321, 430, 633, 736, 922
Hin6I GCGC 2 cut(s) 482, 916
HinP1I GCGC 2 cut(s) 482, 916
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 2 cut(s) 460, 547
HphI GGTGA 4 cut(s) 42, 172, 359, 503
Hpy166II GTNNAC 2 cut(s) 220, 742
Hpy188I TCNGA 7 cut(s) 167, 189, 244, 517, 676, 711, 956
Hpy188III TCNNGA 1 cut(s) 827
Hpy8I GTNNAC 2 cut(s) 220, 742
HpyAV CCTTC 1 cut(s) 925
HpyCH4III ACNGT 2 cut(s) 707, 993
HpyCH4IV ACGT 1 cut(s) 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 598
HpyF3I CTNAG 7 cut(s) 146, 716, 825, 891, 953, 998, 1004
HpySE526I ACGT 1 cut(s) 310
Hsp92I GRCGYC 1 cut(s) 482
Hsp92II CATG 8 cut(s) 49, 235, 272, 321, 430, 633, 736, 922
HspAI GCGC 2 cut(s) 482, 916
KasI GGCGCC 1 cut(s) 481
Ksp22I TGATCA 2 cut(s) 162, 517
Kzo9I GATC 3 cut(s) 162, 273, 517
LmnI GCTCC 1 cut(s) 866
Lsp1109I GCAGC 6 cut(s) 292, 600, 647, 950, 1008, 1011
LweI GCATC 5 cut(s) 241, 364, 588, 764, 874
MabI ACCWGGT 1 cut(s) 784
MaeI CTAG 1 cut(s) 815
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 3 cut(s) 48, 112, 817
MalI GATC 3 cut(s) 164, 275, 519
MbiI CCGCTC 1 cut(s) 869
MboI GATC 3 cut(s) 162, 273, 517
MboII GAAGA 1 cut(s) 993
MflI RGATCY 1 cut(s) 273
MhlI GDGCHC 1 cut(s) 1010
MluCI AATT 4 cut(s) 121, 173, 449, 506
Mly113I GGCGCC 1 cut(s) 482
MlyI GAGTC 1 cut(s) 541
MmeI TCCRAC 1 cut(s) 844
MnlI CCTC 6 cut(s) 73, 151, 238, 438, 789, 886
Mph1103I ATGCAT 1 cut(s) 587
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 3 cut(s) 225, 288, 543
MslI CAYNNNNRTG 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 941
MspR9I CCNGG 6 cut(s) 74, 201, 436, 786, 909, 975
Mva1269I GAATGC 2 cut(s) 585, 587
MvaI CCWGG 6 cut(s) 74, 201, 436, 786, 909, 975
MwoI GCNNNNNNNGC 1 cut(s) 598
NarI GGCGCC 1 cut(s) 482
NdeII GATC 3 cut(s) 162, 273, 517
NlaIII CATG 8 cut(s) 49, 235, 272, 321, 430, 633, 736, 922
NlaIV GGNNCC 4 cut(s) 483, 571, 777, 897
NmuCI GTSAC 2 cut(s) 48, 817
NsiI ATGCAT 1 cut(s) 587
NspI RCATGY 2 cut(s) 321, 633
PaqCI CACCTGC 1 cut(s) 61
PciI ACATGT 1 cut(s) 629
PctI GAATGC 2 cut(s) 585, 587
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 460
PfoI TCCNGGA 2 cut(s) 199, 907
PkrI GCNGC 6 cut(s) 307, 615, 637, 940, 1023, 1026
PleI GAGTC 1 cut(s) 541
PluTI GGCGCC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 541
Ppu21I YACGTR 1 cut(s) 311
PpuMI RGGWCCY 1 cut(s) 895
PscI ACATGT 1 cut(s) 629
Psp124BI GAGCTC 1 cut(s) 1010
Psp5II RGGWCCY 1 cut(s) 895
Psp6I CCWGG 6 cut(s) 72, 199, 434, 784, 907, 973
PspFI CCCAGC 3 cut(s) 321, 797, 941
PspGI CCWGG 6 cut(s) 72, 199, 434, 784, 907, 973
PspN4I GGNNCC 4 cut(s) 483, 571, 777, 897
PspPI GGNCC 1 cut(s) 895
PspPPI RGGWCCY 1 cut(s) 895
PstI CTGCAG 1 cut(s) 362
PstNI CAGNNNCTG 1 cut(s) 248
PsuI RGATCY 1 cut(s) 273
PvuII CAGCTG 1 cut(s) 941
RsaI GTAC 3 cut(s) 267, 316, 743
RsaNI GTAC 3 cut(s) 266, 315, 742
RseI CAYNNNNRTG 1 cut(s) 737
SacI GAGCTC 1 cut(s) 1010
SaqAI TTAA 3 cut(s) 225, 288, 543
SatI GCNGC 6 cut(s) 306, 614, 636, 939, 1022, 1025
Sau3AI GATC 3 cut(s) 162, 273, 517
Sau96I GGNCC 1 cut(s) 895
SchI GAGTC 1 cut(s) 541
ScrFI CCNGG 6 cut(s) 74, 201, 436, 786, 909, 975
SduI GDGCHC 1 cut(s) 1010
SexAI ACCWGGT 1 cut(s) 784
SfaNI GCATC 5 cut(s) 241, 364, 588, 764, 874
SfcI CTRYAG 2 cut(s) 358, 369
SfoI GGCGCC 1 cut(s) 483
SinI GGWCC 1 cut(s) 895
SmiMI CAYNNNNRTG 1 cut(s) 737
SmlI CTYRAG 2 cut(s) 255, 498
SmoI CTYRAG 2 cut(s) 255, 498
Sse9I AATT 4 cut(s) 121, 173, 449, 506
SsiI CCGC 2 cut(s) 760, 869
SspDI GGCGCC 1 cut(s) 481
SspMI CTAG 1 cut(s) 815
SstI GAGCTC 1 cut(s) 1010
StyD4I CCNGG 6 cut(s) 72, 199, 434, 784, 907, 973
TaaI ACNGT 2 cut(s) 707, 993
TaiI ACGT 1 cut(s) 313
TasI AATT 4 cut(s) 121, 173, 449, 506
TatI WGTACW 3 cut(s) 265, 314, 741
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 3 cut(s) 225, 288, 543
Tru9I TTAA 3 cut(s) 225, 288, 543
TscAI CASTG 3 cut(s) 63, 599, 744
TseFI GTSAC 2 cut(s) 48, 817
TseI GCWGC 6 cut(s) 305, 613, 635, 938, 1021, 1024
Tsp45I GTSAC 2 cut(s) 48, 817
TspDTI ATGAA 4 cut(s) 34, 71, 228, 944
TspRI CASTG 3 cut(s) 63, 599, 744
VpaK11BI GGWCC 1 cut(s) 895
XapI RAATTY 2 cut(s) 121, 173
XceI RCATGY 2 cut(s) 321, 633
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 1 cut(s) 815
Zsp2I ATGCAT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.