RLG00000013043

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0019028
Physical Location & Seq
Forward (+)
25328779 .. 25331628
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013043

Sequence Viewer

Length: 915 bp
ATGGAAAGAAGACCAGTAGCTGATGTACCTGATGAAAATAAACTTTGGCAACTGGTAGAGATTGTTGATCCTCTTCACTGTCTAGTGGTTACGATGCCGTATAGTAAAGAACCTGCCAATAAGGTAATAACAACTTTCATGTCACCTGCACTGGCCTCAACCTACCTGGCATTTCATCCTTTGAACAGCAACATCATAACGATTGTAACAGAAAATTCAATCATTCACATTTACTATCTTAGAGTTGATGAGGTGATCAGACCAAAATTCAAGTGCCATCAGAAACACATTTCTGGATTTGCATTTTCTGTCAACCTTAAAGTCATGGTTTCATCAGAGGCTGATGCTCAAGCTCAACTACGCTTTTGGAACATGGATACCTGGGATAAGAGGAAATTAGTTAGGATTCAACTGCCAACTGGAAAGGCGCCTACTGGTGATACTCAAGTGCAATTCTACTCTGATCAAATCCTGTTGTTGGTATTGGTTAAGACTCAACCAGCACTATATGATGGTGCCAAAACAGAATGCATTCAACAGTGGATGCCACGAGATGTGCTGCCTGCACCGATAACATGTGCAGCATATTCCCCGACTAATCAACTCTATGGGGCTTTATCTGATAGCAATGTTGGAGTATTTGATACTGACAGTCTGAAACTTGGATGTCGTATAGCCATGTCAGTGTACTTTGTAGAAGCATCCTGCAACAGGTTCAAGGTTTTTCAACCTTGTTCAAACACTGGGATTGGTGCTGTGCTAGTGACTGCTCAGGAAGTGGAAGCTCACAGTGAAAAGGGTCTGCTTGTTGGAGCGGTTGCAGAGAGTGATGCTACTGAGGACCCCATTTTTCCTGGAGAGCGCATGAGCAAGATGAGGAGCACAGTTATGGTATCTCTCAAGTATTTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

305

Amino Acids

34.05

Weight (kDa)

6.31

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019028)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0280621
rosa_laevigata RLG00000013043
rosa_roxburghii Rroxscaffold_7G00187870
rosa_rugosa Rorug06G0132900
rosa_samantha Rh6AG245500 Rh6BG247800 Rh6CG249300 Rh6DG240500
rosa_wichuraiana Rw6G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 154
Acc36I ACCTGC 2 cut(s) 121, 154
AccB1I GGYRCC 2 cut(s) 427, 515
AccBSI CCGCTC 1 cut(s) 815
AciI CCGC 1 cut(s) 815
AclWI GGATC 1 cut(s) 62
AcsI RAATTY 2 cut(s) 214, 266
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 2 cut(s) 27, 689
AfiI CCNNNNNNNGG 2 cut(s) 478, 711
AflIII ACRYGT 1 cut(s) 575
AgsI TTSAA 8 cut(s) 184, 219, 271, 410, 536, 718, 728, 738
AjnI CCWGG 3 cut(s) 165, 380, 853
AluBI AGCT 3 cut(s) 20, 353, 785
AluI AGCT 3 cut(s) 20, 353, 785
Alw21I GWGCWC 1 cut(s) 884
AlwI GGATC 1 cut(s) 62
AlwNI CAGNNNCTG 2 cut(s) 20, 341
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 2 cut(s) 559, 581
ApoI RAATTY 2 cut(s) 214, 266
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 2 cut(s) 430, 864
AspS9I GGNCC 1 cut(s) 841
AsuHPI GGTGA 3 cut(s) 135, 265, 449
AvaII GGWCC 1 cut(s) 841
BaeI ACNNNNGTAYC 2 cut(s) 432, 465
BanI GGYRCC 2 cut(s) 427, 515
BauI CACGAG 1 cut(s) 549
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 1 cut(s) 884
BbvI GCAGC 2 cut(s) 546, 593
BccI CCATC 2 cut(s) 285, 506
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 3 cut(s) 167, 382, 855
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 2 cut(s) 255, 463
BfaI CTAG 2 cut(s) 83, 761
BfoI RGCGCY 1 cut(s) 431
BfuAI ACCTGC 2 cut(s) 121, 154
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 2 cut(s) 560, 582
BlsI GCNGC 2 cut(s) 561, 583
Bme1390I CCNGG 3 cut(s) 167, 382, 855
Bme18I GGWCC 1 cut(s) 841
BmgT120I GGNCC 1 cut(s) 841
BmiI GGNNCC 3 cut(s) 429, 517, 843
BmrFI CCNGG 3 cut(s) 167, 382, 855
BmrI ACTGGG 1 cut(s) 753
BmsI GCATC 5 cut(s) 84, 334, 534, 710, 820
BmuI ACTGGG 1 cut(s) 753
BpiI GAAGAC 1 cut(s) 16
BpmI CTGGAG 1 cut(s) 876
Bpu10I CCTNAGC 1 cut(s) 771
BpuEI CTTGAG 3 cut(s) 333, 429, 884
BsaHI GRCGYC 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 381
Bsc4I CCNNNNNNNGG 2 cut(s) 478, 711
Bse1I ACTGG 6 cut(s) 14, 57, 156, 424, 439, 748
Bse3DI GCAATG 1 cut(s) 634
BseBI CCWGG 3 cut(s) 167, 382, 855
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 4 cut(s) 175, 549, 671, 701
BseLI CCNNNNNNNGG 2 cut(s) 478, 711
BseMI GCAATG 1 cut(s) 634
BseMII CTCAG 2 cut(s) 785, 828
BseNI ACTGG 6 cut(s) 14, 57, 156, 424, 439, 748
BseRI GAGGAG 1 cut(s) 892
BseXI GCAGC 2 cut(s) 546, 593
BsgI GTGCAG 3 cut(s) 132, 549, 600
BshFI GGCC 1 cut(s) 155
BshNI GGYRCC 2 cut(s) 427, 515
BsiHKAI GWGCWC 1 cut(s) 884
BslI CCNNNNNNNGG 2 cut(s) 478, 711
BsmI GAATGC 2 cut(s) 531, 533
BsnI GGCC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 884
Bsp143I GATC 3 cut(s) 67, 255, 463
BspACI CCGC 1 cut(s) 815
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 2 cut(s) 784, 829
BspLI GGNNCC 3 cut(s) 429, 517, 843
BspMI ACCTGC 2 cut(s) 121, 154
BspPI GGATC 1 cut(s) 62
BspT107I GGYRCC 2 cut(s) 427, 515
BsrBI CCGCTC 1 cut(s) 815
BsrDI GCAATG 1 cut(s) 634
BsrI ACTGG 6 cut(s) 14, 57, 156, 424, 439, 748
BssECI CCNNGG 1 cut(s) 381
BssMI GATC 3 cut(s) 67, 255, 463
BssNI GRCGYC 1 cut(s) 428
BssSI CACGAG 1 cut(s) 549
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 3 cut(s) 167, 382, 855
Bst4CI ACNGT 5 cut(s) 80, 540, 653, 791, 886
Bst6I CTCTTC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 564
BstDEI CTNAG 3 cut(s) 239, 771, 837
BstENI CCTNNNNNAGG 1 cut(s) 709
BstF5I GGATG 4 cut(s) 175, 549, 671, 701
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 2 cut(s) 430, 864
BstKTI GATC 3 cut(s) 70, 258, 466
BstMBI GATC 3 cut(s) 67, 255, 463
BstNI CCWGG 3 cut(s) 167, 382, 855
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 3 cut(s) 165, 380, 853
BstV1I GCAGC 2 cut(s) 546, 593
BstV2I GAAGAC 1 cut(s) 16
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 1 cut(s) 155
BtsCI GGATG 4 cut(s) 175, 549, 671, 701
BtsIMutI CAGTG 6 cut(s) 76, 149, 545, 690, 741, 796
BveI ACCTGC 2 cut(s) 121, 154
Cac8I GCNNGC 1 cut(s) 564
CaiI CAGNNNCTG 2 cut(s) 20, 341
CfoI GCGC 2 cut(s) 430, 864
Cfr13I GGNCC 1 cut(s) 841
Csp6I GTAC 2 cut(s) 26, 688
CviAII CATG 6 cut(s) 139, 325, 373, 576, 679, 865
CviJI RGCY 7 cut(s) 20, 155, 341, 353, 614, 677, 785
CviKI_1 RGCY 7 cut(s) 20, 155, 341, 353, 614, 677, 785
CviQI GTAC 2 cut(s) 26, 688
DdeI CTNAG 3 cut(s) 239, 771, 837
DinI GGCGCC 1 cut(s) 429
DpnI GATC 3 cut(s) 69, 257, 465
DpnII GATC 3 cut(s) 67, 255, 463
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 841
EcoNI CCTNNNNNAGG 1 cut(s) 709
EcoO109I RGGNCCY 1 cut(s) 841
EcoRII CCWGG 3 cut(s) 165, 380, 853
EcoT22I ATGCAT 1 cut(s) 533
EgeI GGCGCC 1 cut(s) 429
EheI GGCGCC 1 cut(s) 429
FaeI CATG 6 cut(s) 142, 328, 376, 579, 682, 868
FatI CATG 6 cut(s) 138, 324, 372, 575, 678, 864
FbaI TGATCA 2 cut(s) 255, 463
Fnu4HI GCNGC 2 cut(s) 560, 582
FokI GGATG 4 cut(s) 162, 556, 678, 688
Fsp4HI GCNGC 2 cut(s) 560, 582
FspBI CTAG 2 cut(s) 83, 761
GlaI GCGC 2 cut(s) 429, 863
GluI GCNGC 2 cut(s) 560, 582
GsuI CTGGAG 1 cut(s) 876
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 1 cut(s) 155
HhaI GCGC 2 cut(s) 430, 864
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 6 cut(s) 142, 328, 376, 579, 682, 868
Hin6I GCGC 2 cut(s) 428, 862
HinP1I GCGC 2 cut(s) 428, 862
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 2 cut(s) 406, 493
HphI GGTGA 3 cut(s) 135, 265, 449
Hpy166II GTNNAC 2 cut(s) 313, 688
Hpy188I TCNGA 6 cut(s) 260, 282, 337, 463, 622, 657
Hpy188III TCNNGA 2 cut(s) 294, 773
Hpy8I GTNNAC 2 cut(s) 313, 688
HpyCH4III ACNGT 5 cut(s) 80, 540, 653, 791, 886
HpyCH4V TGCA 8 cut(s) 149, 302, 451, 531, 566, 581, 708, 821
HpyF3I CTNAG 3 cut(s) 239, 771, 837
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 6 cut(s) 142, 328, 376, 579, 682, 868
HspAI GCGC 2 cut(s) 428, 862
KasI GGCGCC 1 cut(s) 427
Ksp22I TGATCA 2 cut(s) 255, 463
Kzo9I GATC 3 cut(s) 67, 255, 463
LmnI GCTCC 2 cut(s) 812, 879
Lsp1109I GCAGC 2 cut(s) 546, 593
LweI GCATC 5 cut(s) 84, 334, 534, 710, 820
MaeI CTAG 2 cut(s) 83, 761
MaeIII GTNAC 4 cut(s) 88, 141, 205, 763
MalI GATC 3 cut(s) 69, 257, 465
MbiI CCGCTC 1 cut(s) 815
MboI GATC 3 cut(s) 67, 255, 463
MboII GAAGA 2 cut(s) 21, 65
MhlI GDGCHC 1 cut(s) 884
MluCI AATT 4 cut(s) 214, 266, 395, 452
Mly113I GGCGCC 1 cut(s) 428
MlyI GAGTC 1 cut(s) 487
MmeI TCCRAC 2 cut(s) 613, 790
MnlI CCTC 7 cut(s) 81, 166, 244, 331, 384, 832, 870
Mph1103I ATGCAT 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 531
MseI TTAA 2 cut(s) 318, 489
MslI CAYNNNNRTG 2 cut(s) 683, 887
MspR9I CCNGG 3 cut(s) 167, 382, 855
Mva1269I GAATGC 2 cut(s) 531, 533
MvaI CCWGG 3 cut(s) 167, 382, 855
NarI GGCGCC 1 cut(s) 428
NdeII GATC 3 cut(s) 67, 255, 463
NlaIII CATG 6 cut(s) 142, 328, 376, 579, 682, 868
NlaIV GGNNCC 3 cut(s) 429, 517, 843
NmuCI GTSAC 2 cut(s) 141, 763
NsiI ATGCAT 1 cut(s) 533
NspI RCATGY 1 cut(s) 579
PaqCI CACCTGC 1 cut(s) 154
PciI ACATGT 1 cut(s) 575
PctI GAATGC 2 cut(s) 531, 533
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 1 cut(s) 406
PfoI TCCNGGA 1 cut(s) 853
PkrI GCNGC 2 cut(s) 561, 583
PleI GAGTC 1 cut(s) 487
PluTI GGCGCC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 841
PscI ACATGT 1 cut(s) 575
Psp5II RGGWCCY 1 cut(s) 841
Psp6I CCWGG 3 cut(s) 165, 380, 853
PspGI CCWGG 3 cut(s) 165, 380, 853
PspN4I GGNNCC 3 cut(s) 429, 517, 843
PspPI GGNCC 1 cut(s) 841
PspPPI RGGWCCY 1 cut(s) 841
PstNI CAGNNNCTG 2 cut(s) 20, 341
RsaI GTAC 2 cut(s) 27, 689
RsaNI GTAC 2 cut(s) 26, 688
RseI CAYNNNNRTG 2 cut(s) 683, 887
SaqAI TTAA 2 cut(s) 318, 489
SatI GCNGC 2 cut(s) 560, 582
Sau3AI GATC 3 cut(s) 67, 255, 463
Sau96I GGNCC 1 cut(s) 841
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 3 cut(s) 167, 382, 855
SduI GDGCHC 1 cut(s) 884
SfaNI GCATC 5 cut(s) 84, 334, 534, 710, 820
SfoI GGCGCC 1 cut(s) 429
SinI GGWCC 1 cut(s) 841
SmiMI CAYNNNNRTG 2 cut(s) 683, 887
SmlI CTYRAG 3 cut(s) 348, 444, 899
SmoI CTYRAG 3 cut(s) 348, 444, 899
Sse9I AATT 4 cut(s) 214, 266, 395, 452
SsiI CCGC 1 cut(s) 815
SspDI GGCGCC 1 cut(s) 427
SspMI CTAG 2 cut(s) 83, 761
StyD4I CCNGG 3 cut(s) 165, 380, 853
TaaI ACNGT 5 cut(s) 80, 540, 653, 791, 886
TasI AATT 4 cut(s) 214, 266, 395, 452
TatI WGTACW 1 cut(s) 687
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 2 cut(s) 318, 489
Tru9I TTAA 2 cut(s) 318, 489
TscAI CASTG 6 cut(s) 83, 156, 545, 690, 748, 796
TseFI GTSAC 2 cut(s) 141, 763
TseI GCWGC 2 cut(s) 559, 581
Tsp45I GTSAC 2 cut(s) 141, 763
TspDTI ATGAA 4 cut(s) 48, 127, 164, 321
TspRI CASTG 6 cut(s) 83, 156, 545, 690, 748, 796
VpaK11BI GGWCC 1 cut(s) 841
XagI CCTNNNNNAGG 1 cut(s) 709
XapI RAATTY 2 cut(s) 214, 266
XceI RCATGY 1 cut(s) 579
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 2 cut(s) 83, 761
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.