Rroxscaffold_7G00187870

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0019028
Physical Location & Seq
Forward (+)
27300091 .. 27301790
1700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187870.1

Sequence Viewer

Length: 618 bp
ATGGATACCTGGGATAAGAAGAAATTTGTTAGGATTCGACTGCCAACTGAAAAGGCGCCTACTGGTGATACTGAAGTGCAATTCTACTCTGATCAAATCCTGTTGTTGGTATTGGTTAAGACTCAACCAGCACTATATGATGGTTCCAAAACAGAAAGCATTCAACAGTGGATGCCACGAGATGTGCTGCCTGCACCGATAACATGTGCAGCATATTCCCCGAATAATCAACTCTATGGGGCTTTATTTGATAGCAATGTTGGAGTATTTGATACTGACAATCTGAAACTTAGATGTCGTAAAGCCATGTCAGTGTACTTTGTAGAAGCATCCCGCAACAGGTTCAAGGTTCCTCAACCTGGTTCAAATACTGGGATTGGTGCTGTGCTAGTGACTGCTCAGGAAGTGGAAGCTCATAGTGAAAAGGGTCTGCTTGTTGGAGCTGTTGCAGAGAGTGATGCTACTGAGGACCCCATTTTTCCTGGAGAGCGCGTGAGCAAGATGAGGAGCGCAGTTGGGGTGTCTCTCAAGAGTATTTTTGGCGACACCAGGAGGAAGATTTTTACTGTTGCTGAGACTGAGCTCAACAAAACTGCTGCTGATCTCCAAGGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

205

Amino Acids

22.53

Weight (kDa)

6.61

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019028)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0280621
rosa_laevigata RLG00000013043
rosa_roxburghii Rroxscaffold_7G00187870
rosa_rugosa Rorug06G0132900
rosa_samantha Rh6AG245500 Rh6BG247800 Rh6CG249300 Rh6DG240500
rosa_wichuraiana Rw6G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 55
AccII CGCG 1 cut(s) 492
AciI CCGC 1 cut(s) 334
AcsI RAATTY 1 cut(s) 23
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 3 cut(s) 106, 339, 359
AflIII ACRYGT 1 cut(s) 203
AgsI TTSAA 3 cut(s) 164, 346, 366
AjnI CCWGG 4 cut(s) 8, 358, 481, 548
AluBI AGCT 3 cut(s) 413, 443, 583
AluI AGCT 3 cut(s) 413, 443, 583
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 2 cut(s) 528, 569
ApeKI GCWGC 3 cut(s) 187, 209, 596
ApoI RAATTY 1 cut(s) 23
Asp700I GAANNNNTTC 1 cut(s) 159
AspLEI GCGC 3 cut(s) 58, 492, 512
AspS9I GGNCC 1 cut(s) 469
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 469
BaeI ACNNNNGTAYC 2 cut(s) 60, 93
BanI GGYRCC 1 cut(s) 55
BanII GRGCYC 1 cut(s) 585
BauI CACGAG 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 3 cut(s) 174, 221, 583
BccI CCATC 1 cut(s) 134
BciT130I CCWGG 4 cut(s) 10, 360, 483, 550
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 2 cut(s) 528, 569
BfaI CTAG 1 cut(s) 389
BfoI RGCGCY 1 cut(s) 59
BisI GCNGC 3 cut(s) 188, 210, 597
BlsI GCNGC 3 cut(s) 189, 211, 598
Bme1390I CCNGG 4 cut(s) 10, 360, 483, 550
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 4 cut(s) 57, 145, 351, 471
BmrFI CCNGG 4 cut(s) 10, 360, 483, 550
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 3 cut(s) 162, 338, 448
BmuI ACTGGG 1 cut(s) 381
BpmI CTGGAG 1 cut(s) 504
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 512
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 9, 607
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 339, 359
Bse1I ACTGG 2 cut(s) 67, 376
Bse3DI GCAATG 1 cut(s) 262
BseBI CCWGG 4 cut(s) 10, 360, 483, 550
BseDI CCNNGG 2 cut(s) 9, 607
BseGI GGATG 2 cut(s) 177, 329
BseLI CCNNNNNNNGG 3 cut(s) 106, 339, 359
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 4 cut(s) 413, 456, 564, 570
BseNI ACTGG 2 cut(s) 67, 376
BseRI GAGGAG 1 cut(s) 520
BseXI GCAGC 3 cut(s) 174, 221, 583
BsgI GTGCAG 2 cut(s) 177, 228
Bsh1236I CGCG 1 cut(s) 492
BshNI GGYRCC 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 585
BslI CCNNNNNNNGG 3 cut(s) 106, 339, 359
BsmAI GTCTC 2 cut(s) 528, 569
BsmI GAATGC 1 cut(s) 159
Bsp1286I GDGCHC 1 cut(s) 585
Bsp143I GATC 2 cut(s) 91, 601
BspACI CCGC 1 cut(s) 334
BspCNI CTCAG 4 cut(s) 412, 457, 565, 571
BspFNI CGCG 1 cut(s) 492
BspLI GGNNCC 4 cut(s) 57, 145, 351, 471
BspT107I GGYRCC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 2 cut(s) 67, 376
BssECI CCNNGG 2 cut(s) 9, 607
BssMI GATC 2 cut(s) 91, 601
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 177
BssT1I CCWWGG 1 cut(s) 607
Bst2BI CACGAG 1 cut(s) 177
Bst2UI CCWGG 4 cut(s) 10, 360, 483, 550
Bst4CI ACNGT 2 cut(s) 168, 568
BstACI GRCGYC 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 5 cut(s) 290, 399, 465, 573, 579
BstF5I GGATG 2 cut(s) 177, 329
BstFNI CGCG 1 cut(s) 492
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 3 cut(s) 58, 492, 512
BstKTI GATC 2 cut(s) 94, 604
BstMAI GTCTC 2 cut(s) 528, 569
BstMBI GATC 2 cut(s) 91, 601
BstNI CCWGG 4 cut(s) 10, 360, 483, 550
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 4 cut(s) 8, 358, 481, 548
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 174, 221, 583
BtsCI GGATG 2 cut(s) 177, 329
BtsIMutI CAGTG 2 cut(s) 173, 318
Cac8I GCNNGC 1 cut(s) 192
CfoI GCGC 3 cut(s) 58, 492, 512
Cfr13I GGNCC 1 cut(s) 469
CsiI ACCWGGT 1 cut(s) 358
Csp6I GTAC 1 cut(s) 316
CviAII CATG 2 cut(s) 204, 307
CviJI RGCY 5 cut(s) 242, 305, 413, 443, 583
CviKI_1 RGCY 5 cut(s) 242, 305, 413, 443, 583
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 5 cut(s) 290, 399, 465, 573, 579
DinI GGCGCC 1 cut(s) 57
DpnI GATC 2 cut(s) 93, 603
DpnII GATC 2 cut(s) 91, 601
Ecl136II GAGCTC 1 cut(s) 583
Eco130I CCWWGG 1 cut(s) 607
Eco24I GRGCYC 1 cut(s) 585
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 583
Eco57I CTGAAG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 583
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 4 cut(s) 8, 358, 481, 548
EcoT14I CCWWGG 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 585
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 607
FaeI CATG 2 cut(s) 207, 310
FaiI YATR 7 cut(s) 136, 138, 205, 214, 237, 308, 417
FatI CATG 2 cut(s) 203, 306
FauI CCCGC 1 cut(s) 341
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 3 cut(s) 188, 210, 597
FokI GGATG 2 cut(s) 184, 316
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 3 cut(s) 188, 210, 597
FspBI CTAG 1 cut(s) 389
GlaI GCGC 3 cut(s) 57, 491, 511
GluI GCNGC 3 cut(s) 188, 210, 597
GsuI CTGGAG 1 cut(s) 504
HaeII RGCGCY 1 cut(s) 59
HhaI GCGC 3 cut(s) 58, 492, 512
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 2 cut(s) 207, 310
Hin6I GCGC 3 cut(s) 56, 490, 510
HinP1I GCGC 3 cut(s) 56, 490, 510
HinfI GANTC 2 cut(s) 34, 121
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 2 cut(s) 91, 285
Hpy188III TCNNGA 2 cut(s) 401, 529
Hpy8I GTNNAC 1 cut(s) 316
HpyCH4III ACNGT 2 cut(s) 168, 568
HpyCH4V TGCA 4 cut(s) 79, 194, 209, 449
HpyF3I CTNAG 5 cut(s) 290, 399, 465, 573, 579
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 2 cut(s) 207, 310
HspAI GCGC 3 cut(s) 56, 490, 510
KasI GGCGCC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 2 cut(s) 91, 601
LmnI GCTCC 2 cut(s) 440, 507
Lsp1109I GCAGC 3 cut(s) 174, 221, 583
LweI GCATC 3 cut(s) 162, 338, 448
MabI ACCWGGT 1 cut(s) 358
MaeI CTAG 1 cut(s) 389
MaeIII GTNAC 1 cut(s) 391
MalI GATC 2 cut(s) 93, 603
MboI GATC 2 cut(s) 91, 601
MboII GAAGA 2 cut(s) 31, 568
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 2 cut(s) 23, 80
Mly113I GGCGCC 1 cut(s) 56
MlyI GAGTC 1 cut(s) 115
MmeI TCCRAC 2 cut(s) 241, 418
MnlI CCTC 4 cut(s) 363, 460, 498, 546
MroXI GAANNNNTTC 1 cut(s) 159
MseI TTAA 1 cut(s) 117
MslI CAYNNNNRTG 1 cut(s) 311
MspR9I CCNGG 4 cut(s) 10, 360, 483, 550
Mva1269I GAATGC 1 cut(s) 159
MvaI CCWGG 4 cut(s) 10, 360, 483, 550
MvnI CGCG 1 cut(s) 492
NarI GGCGCC 1 cut(s) 56
NdeII GATC 2 cut(s) 91, 601
NlaIII CATG 2 cut(s) 207, 310
NlaIV GGNNCC 4 cut(s) 57, 145, 351, 471
NmuCI GTSAC 1 cut(s) 391
NspI RCATGY 1 cut(s) 207
PciI ACATGT 1 cut(s) 203
PctI GAATGC 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 34
PfoI TCCNGGA 1 cut(s) 481
PkrI GCNGC 3 cut(s) 189, 211, 598
PleI GAGTC 1 cut(s) 115
PluTI GGCGCC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 115
PpuMI RGGWCCY 1 cut(s) 469
PscI ACATGT 1 cut(s) 203
Psp124BI GAGCTC 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 469
Psp6I CCWGG 4 cut(s) 8, 358, 481, 548
PspGI CCWGG 4 cut(s) 8, 358, 481, 548
PspN4I GGNNCC 4 cut(s) 57, 145, 351, 471
PspPI GGNCC 1 cut(s) 469
PspPPI RGGWCCY 1 cut(s) 469
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 311
SacI GAGCTC 1 cut(s) 585
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 3 cut(s) 188, 210, 597
Sau3AI GATC 2 cut(s) 91, 601
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 4 cut(s) 10, 360, 483, 550
SduI GDGCHC 1 cut(s) 585
SetI ASST 7 cut(s) 11, 344, 351, 361, 415, 445, 585
SexAI ACCWGGT 1 cut(s) 358
SfaNI GCATC 3 cut(s) 162, 338, 448
SfoI GGCGCC 1 cut(s) 57
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
Sse9I AATT 2 cut(s) 23, 80
SsiI CCGC 1 cut(s) 334
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 1 cut(s) 389
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 4 cut(s) 8, 358, 481, 548
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 2 cut(s) 168, 568
TaqI TCGA 1 cut(s) 37
TasI AATT 2 cut(s) 23, 80
TatI WGTACW 1 cut(s) 315
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 2 cut(s) 173, 318
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 3 cut(s) 187, 209, 596
Tsp45I GTSAC 1 cut(s) 391
TspRI CASTG 2 cut(s) 173, 318
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 1 cut(s) 23
XceI RCATGY 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.