Rw6G021250

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0019028
Physical Location & Seq
Reverse (-)
42475910 .. 42479302
3393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G021250.1

Sequence Viewer

Length: 633 bp
ATGAAACTTCTAGCAACTTCGACGACACCCATCGTCCCGTCATTTTCGTTTGACCATACACAAAAGCGTTTGGTAATAACAACTTTCATGTCACCTGCACTGGCCTCAACCTACCTGGCATTTCATCCTTTGAACAACAATATCATAACGATTGTAACAGAAAATTCAATCATTCACATTTACTATCTTAGAGTTGATGAGGTGATCAGACCAAAATTCAAGTGCCATCAGAAACACATTCCTGCATTTGCATTTTCTGTCAACCTTAAAGTCATGGTTTCATCAGAGGCTGATGCTCAAGCTCAATGGATGCCACGAGATGTGCTGCCTGCACCGATAACATGTGCAGCATATTCCCCGAATAATCAACTCTATGGGGCTTTATCTGATAGCAATGTTGGAGTATTTGATACTGACAGTCTGAAACTTAGATGTCGTATAGCCATGTCAGTGTACTTTGTAGAAGCATCCTGCAACAGGTTCAAGGTTCCTCAACCTGGTTCAAACGCTGGGATTGGTGCTGTGCTAGTGACTGCTCAGGAAGTGGAAGCTCATAGTGAAAAGGGTCTGCTTGTTGGAGCGGTTGCAGAGAGTGATGCTACTGAGGACCCCATTTTCCCGGAGAGCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

210

Amino Acids

22.95

Weight (kDa)

6.05

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019028)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0280621
rosa_laevigata RLG00000013043
rosa_roxburghii Rroxscaffold_7G00187870
rosa_rugosa Rorug06G0132900
rosa_samantha Rh6AG245500 Rh6BG247800 Rh6CG249300 Rh6DG240500
rosa_wichuraiana Rw6G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 103
AccBSI CCGCTC 1 cut(s) 581
AciI CCGC 1 cut(s) 581
AcsI RAATTY 2 cut(s) 163, 215
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 2 cut(s) 477, 497
AflIII ACRYGT 1 cut(s) 341
AgsI TTSAA 5 cut(s) 133, 168, 220, 484, 504
AjnI CCWGG 2 cut(s) 114, 496
AluBI AGCT 2 cut(s) 302, 551
AluI AGCT 2 cut(s) 302, 551
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 2 cut(s) 325, 347
ApoI RAATTY 2 cut(s) 163, 215
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 1 cut(s) 607
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 2 cut(s) 84, 214
AvaII GGWCC 1 cut(s) 607
BauI CACGAG 1 cut(s) 315
BbvI GCAGC 2 cut(s) 312, 359
BccI CCATC 2 cut(s) 38, 234
BciT130I CCWGG 2 cut(s) 116, 498
BclI TGATCA 1 cut(s) 204
BcnI CCSGG 1 cut(s) 620
BfaI CTAG 2 cut(s) 11, 527
BfuAI ACCTGC 1 cut(s) 103
BisI GCNGC 2 cut(s) 326, 348
BlsI GCNGC 2 cut(s) 327, 349
Bme1390I CCNGG 3 cut(s) 116, 498, 620
Bme18I GGWCC 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 607
BmiI GGNNCC 2 cut(s) 489, 609
BmrFI CCNGG 3 cut(s) 116, 498, 620
BmsI GCATC 4 cut(s) 283, 300, 476, 586
Bpu10I CCTNAGC 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 620
Bsc4I CCNNNNNNNGG 2 cut(s) 477, 497
Bse1I ACTGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 400
BseBI CCWGG 2 cut(s) 116, 498
BseGI GGATG 3 cut(s) 124, 315, 467
BseLI CCNNNNNNNGG 2 cut(s) 477, 497
BseMI GCAATG 1 cut(s) 400
BseMII CTCAG 2 cut(s) 551, 594
BseNI ACTGG 1 cut(s) 105
BseXI GCAGC 2 cut(s) 312, 359
BseYI CCCAGC 1 cut(s) 509
BsgI GTGCAG 3 cut(s) 81, 315, 366
BshFI GGCC 1 cut(s) 104
BsiSI CCGG 1 cut(s) 620
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 2 cut(s) 477, 497
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 1 cut(s) 104
Bsp143I GATC 1 cut(s) 204
BspACI CCGC 1 cut(s) 581
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 2 cut(s) 550, 595
BspLI GGNNCC 2 cut(s) 489, 609
BspMI ACCTGC 1 cut(s) 103
BsrBI CCGCTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 400
BsrI ACTGG 1 cut(s) 105
BssMI GATC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 116, 498
Bst4CI ACNGT 1 cut(s) 419
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 4 cut(s) 188, 428, 537, 603
BstENI CCTNNNNNAGG 1 cut(s) 475
BstF5I GGATG 3 cut(s) 124, 315, 467
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 1 cut(s) 207
BstMBI GATC 1 cut(s) 204
BstNI CCWGG 2 cut(s) 116, 498
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 3 cut(s) 114, 496, 618
BstV1I GCAGC 2 cut(s) 312, 359
BsuRI GGCC 1 cut(s) 104
BtsCI GGATG 3 cut(s) 124, 315, 467
BtsIMutI CAGTG 2 cut(s) 98, 456
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 1 cut(s) 330
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 629
Cfr13I GGNCC 1 cut(s) 607
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 1 cut(s) 454
CviAII CATG 5 cut(s) 88, 274, 342, 445, 630
CviJI RGCY 6 cut(s) 104, 290, 302, 380, 443, 551
CviKI_1 RGCY 6 cut(s) 104, 290, 302, 380, 443, 551
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 4 cut(s) 188, 428, 537, 603
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 607
EcoNI CCTNNNNNAGG 1 cut(s) 475
EcoO109I RGGNCCY 1 cut(s) 607
EcoRII CCWGG 2 cut(s) 114, 496
FaeI CATG 5 cut(s) 91, 277, 345, 448, 633
FaqI GGGAC 1 cut(s) 20
FatI CATG 5 cut(s) 87, 273, 341, 444, 629
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 326, 348
FokI GGATG 3 cut(s) 111, 322, 454
Fsp4HI GCNGC 2 cut(s) 326, 348
FspBI CTAG 2 cut(s) 11, 527
GlaI GCGC 1 cut(s) 628
GluI GCNGC 2 cut(s) 326, 348
GsaI CCCAGC 1 cut(s) 513
HaeIII GGCC 1 cut(s) 104
HapII CCGG 1 cut(s) 620
HhaI GCGC 1 cut(s) 629
Hin1II CATG 5 cut(s) 91, 277, 345, 448, 633
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HpaII CCGG 1 cut(s) 620
HphI GGTGA 2 cut(s) 84, 214
Hpy166II GTNNAC 2 cut(s) 262, 454
Hpy188I TCNGA 5 cut(s) 209, 231, 286, 388, 423
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 2 cut(s) 262, 454
Hpy99I CGWCG 1 cut(s) 25
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 7 cut(s) 98, 245, 251, 332, 347, 474, 587
HpyF3I CTNAG 4 cut(s) 188, 428, 537, 603
Hsp92II CATG 5 cut(s) 91, 277, 345, 448, 633
HspAI GCGC 1 cut(s) 627
Ksp22I TGATCA 1 cut(s) 204
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 578
Lsp1109I GCAGC 2 cut(s) 312, 359
LweI GCATC 4 cut(s) 283, 300, 476, 586
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 2 cut(s) 11, 527
MaeIII GTNAC 3 cut(s) 90, 154, 529
MalI GATC 1 cut(s) 206
MbiI CCGCTC 1 cut(s) 581
MboI GATC 1 cut(s) 204
MluCI AATT 2 cut(s) 163, 215
MmeI TCCRAC 2 cut(s) 379, 556
MnlI CCTC 5 cut(s) 115, 193, 280, 501, 598
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 449
MspI CCGG 1 cut(s) 620
MspR9I CCNGG 3 cut(s) 116, 498, 620
MvaI CCWGG 2 cut(s) 116, 498
NciI CCSGG 1 cut(s) 620
NdeII GATC 1 cut(s) 204
NlaIII CATG 5 cut(s) 91, 277, 345, 448, 633
NlaIV GGNNCC 2 cut(s) 489, 609
NmuCI GTSAC 2 cut(s) 90, 529
NspI RCATGY 1 cut(s) 345
PaqCI CACCTGC 1 cut(s) 103
PciI ACATGT 1 cut(s) 341
PfoI TCCNGGA 1 cut(s) 618
PkrI GCNGC 2 cut(s) 327, 349
PpuMI RGGWCCY 1 cut(s) 607
PscI ACATGT 1 cut(s) 341
Psp5II RGGWCCY 1 cut(s) 607
Psp6I CCWGG 2 cut(s) 114, 496
PspFI CCCAGC 1 cut(s) 509
PspGI CCWGG 2 cut(s) 114, 496
PspN4I GGNNCC 2 cut(s) 489, 609
PspPI GGNCC 1 cut(s) 607
PspPPI RGGWCCY 1 cut(s) 607
PstNI CAGNNNCTG 1 cut(s) 290
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 2 cut(s) 326, 348
Sau3AI GATC 1 cut(s) 204
Sau96I GGNCC 1 cut(s) 607
ScrFI CCNGG 3 cut(s) 116, 498, 620
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 4 cut(s) 283, 300, 476, 586
SinI GGWCC 1 cut(s) 607
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 2 cut(s) 163, 215
SsiI CCGC 1 cut(s) 581
SspMI CTAG 2 cut(s) 11, 527
StyD4I CCNGG 3 cut(s) 114, 496, 618
TaaI ACNGT 1 cut(s) 419
TaqI TCGA 1 cut(s) 20
TasI AATT 2 cut(s) 163, 215
TatI WGTACW 1 cut(s) 453
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 105, 456
TseFI GTSAC 2 cut(s) 90, 529
TseI GCWGC 2 cut(s) 325, 347
Tsp45I GTSAC 2 cut(s) 90, 529
TspDTI ATGAA 4 cut(s) 17, 76, 113, 270
TspRI CASTG 2 cut(s) 105, 456
VpaK11BI GGWCC 1 cut(s) 607
XagI CCTNNNNNAGG 1 cut(s) 475
XapI RAATTY 2 cut(s) 163, 215
XceI RCATGY 1 cut(s) 345
XspI CTAG 2 cut(s) 11, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.