Rorug06G0132900

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0019028
Physical Location & Seq
Reverse (-)
18787853 .. 18788644
792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0132900.1

Sequence Viewer

Length: 792 bp
ATGGAGCTACAAACCCAGCAACAAATCGAGGATGTAGGAAACAACCAAGCAGCCGGGAGTAGTGCCAACACGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGATCAAATAAGAATCCTCAAGGATCTTTACTACGACAAGGGAGTTAAGTGCCCAACTACAGAGCACATTCACGAGATCTGTCTCCAGCTGAACCAGTATGGACAGGTTGAGGGCAAGAACATTTACTTTTGGTTCAAGAACGTCAGGGCTCGAGAGAAGCAGATGAAGAGGTGCAATCAGGCTGCTCAAGTGCCCATGGGAACTAGTTCTCCTGGTACTGGTGGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGTGGATCCATAGATATTAATTTTGGGTCTACTAGTTCTGCTGGTGCTGGTGGATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGATCTCAATTTTGGGCCATGGGTCGACTGCAGTGTTGACCCGTATTTCTCCTCACCCTTCACTACTAGTACTAGTACTACTTTCTTTGGCAGGAATGAAGAACAAACTTCCATGGAACAACGAGGAGAAGATCACCAGGAGATTGAAACTCTTCCTTTGTTCCCCATGCCCGGTGAGGACATCCTTGGCAACCTAAAGACTACTTCCGAGGGAGGTAGCGGCTATGGCTACTATTCCGGTGGCTCGGACGGCTACGACGGTGGCTCCCACATTTCCCTTGAGCTCAGCCTCAACCCCTCCGGGGCTGCTGACTTGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

263

Amino Acids

28.25

Weight (kDa)

4.58

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 30 - 91 1.9e-08 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019028)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0280621
rosa_laevigata RLG00000013043
rosa_roxburghii Rroxscaffold_7G00187870
rosa_rugosa Rorug06G0132900
rosa_samantha Rh6AG245500 Rh6BG247800 Rh6CG249300 Rh6DG240500
rosa_wichuraiana Rw6G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 461
AccI GTMKAC 2 cut(s) 407, 495
AciI CCGC 1 cut(s) 690
AclWI GGATC 9 cut(s) 141, 330, 343, 378, 391, 426, 439, 459, 472
AfaI GTAC 4 cut(s) 91, 328, 541, 547
AfiI CCNNNNNNNGG 4 cut(s) 329, 461, 641, 772
AgsI TTSAA 2 cut(s) 247, 617
AhlI ACTAGT 4 cut(s) 314, 410, 536, 542
AjnI CCWGG 3 cut(s) 92, 322, 606
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AloI GAACNNNNNNTCC 2 cut(s) 304, 336
AluBI AGCT 4 cut(s) 7, 199, 458, 754
AluI AGCT 4 cut(s) 7, 199, 458, 754
Alw21I GWGCWC 2 cut(s) 177, 756
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 9 cut(s) 141, 330, 343, 378, 391, 426, 439, 459, 472
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 2 cut(s) 452, 485
ApeKI GCWGC 3 cut(s) 50, 293, 776
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
AseI ATTAAT 1 cut(s) 396
Asp700I GAANNNNTTC 2 cut(s) 316, 621
AspS9I GGNCC 3 cut(s) 357, 452, 485
AsuC2I CCSGG 3 cut(s) 55, 642, 772
AsuHPI GGTGA 3 cut(s) 516, 596, 656
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 1 cut(s) 357
BaeGI GKGCMC 2 cut(s) 164, 306
BamHI GGATCC 4 cut(s) 335, 383, 431, 464
BanII GRGCYC 2 cut(s) 262, 756
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 2 cut(s) 177, 756
BbvI GCAGC 3 cut(s) 62, 280, 763
BccI CCATC 1 cut(s) 443
BceAI ACGGC 2 cut(s) 72, 736
BciT130I CCWGG 3 cut(s) 94, 324, 608
BcnI CCSGG 3 cut(s) 55, 642, 772
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 4 cut(s) 314, 410, 536, 542
BfaI CTAG 4 cut(s) 315, 411, 537, 543
BfmI CTRYAG 2 cut(s) 168, 499
BglII AGATCT 1 cut(s) 186
BisI GCNGC 4 cut(s) 51, 294, 691, 777
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 4 cut(s) 52, 295, 692, 778
BmcAI AGTACT 2 cut(s) 541, 547
Bme1390I CCNGG 6 cut(s) 55, 94, 324, 608, 642, 772
Bme18I GGWCC 1 cut(s) 357
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 3 cut(s) 357, 452, 485
BmiI GGNNCC 6 cut(s) 337, 358, 385, 433, 466, 736
BmrFI CCNGG 6 cut(s) 55, 94, 324, 608, 642, 772
BpmI CTGGAG 2 cut(s) 93, 179
Bpu1102I GCTNAGC 1 cut(s) 755
BpuEI CTTGAG 3 cut(s) 113, 282, 770
BpuMI CCSGG 3 cut(s) 55, 642, 772
BsaJI CCNNGG 6 cut(s) 306, 488, 582, 655, 678, 771
BsaWI WCCGGW 1 cut(s) 707
BsaXI ACNNNNNCTCC 4 cut(s) 304, 334, 719, 749
Bsc4I CCNNNNNNNGG 4 cut(s) 329, 461, 641, 772
Bse1I ACTGG 4 cut(s) 205, 334, 367, 376
BseBI CCWGG 3 cut(s) 94, 324, 608
BseDI CCNNGG 6 cut(s) 306, 488, 582, 655, 678, 771
BseGI GGATG 2 cut(s) 37, 651
BseLI CCNNNNNNNGG 4 cut(s) 329, 461, 641, 772
BseMII CTCAG 1 cut(s) 769
BseNI ACTGG 4 cut(s) 205, 334, 367, 376
BseRI GAGGAG 2 cut(s) 511, 609
BseSI GKGCMC 2 cut(s) 164, 306
BseXI GCAGC 3 cut(s) 62, 280, 763
BseYI CCCAGC 1 cut(s) 15
BshFI GGCC 2 cut(s) 454, 487
BsiHKAI GWGCWC 2 cut(s) 177, 756
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 4 cut(s) 54, 642, 708, 771
BslI CCNNNNNNNGG 4 cut(s) 329, 461, 641, 772
BsmAI GTCTC 1 cut(s) 197
BsnI GGCC 2 cut(s) 454, 487
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 5 cut(s) 164, 177, 262, 306, 756
Bsp1720I GCTNAGC 1 cut(s) 755
Bsp19I CCATGG 3 cut(s) 306, 488, 582
BspACI CCGC 1 cut(s) 690
BspANI GGCC 2 cut(s) 454, 487
BspCNI CTCAG 1 cut(s) 768
BspLI GGNNCC 6 cut(s) 337, 358, 385, 433, 466, 736
BspMAI CTGCAG 1 cut(s) 503
BspPI GGATC 9 cut(s) 141, 330, 343, 378, 391, 426, 439, 459, 472
BsrI ACTGG 4 cut(s) 205, 334, 367, 376
BssECI CCNNGG 6 cut(s) 306, 488, 582, 655, 678, 771
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 4 cut(s) 306, 488, 582, 655
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 3 cut(s) 94, 324, 608
Bst4CI ACNGT 1 cut(s) 731
Bst6I CTCTTC 2 cut(s) 272, 627
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 2 cut(s) 755, 789
BstDSI CCRYGG 3 cut(s) 306, 488, 582
BstF5I GGATG 2 cut(s) 37, 651
BstMAI GTCTC 1 cut(s) 197
BstMWI GCNNNNNNNGC 3 cut(s) 696, 720, 785
BstNI CCWGG 3 cut(s) 94, 324, 608
BstSCI CCNGG 6 cut(s) 53, 92, 322, 606, 640, 770
BstSFI CTRYAG 2 cut(s) 168, 499
BstSLI GKGCMC 2 cut(s) 164, 306
BstV1I GCAGC 3 cut(s) 62, 280, 763
BstX2I RGATCY 6 cut(s) 133, 186, 335, 383, 431, 464
BstYI RGATCY 6 cut(s) 133, 186, 335, 383, 431, 464
BsuRI GGCC 2 cut(s) 454, 487
BtgI CCRYGG 3 cut(s) 306, 488, 582
BtsCI GGATG 2 cut(s) 37, 651
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 360, 508
Cac8I GCNNGC 1 cut(s) 456
Cfr13I GGNCC 3 cut(s) 357, 452, 485
CsiI ACCWGGT 1 cut(s) 92
Csp6I GTAC 4 cut(s) 90, 327, 540, 546
CviAII CATG 4 cut(s) 307, 489, 583, 637
CviQI GTAC 4 cut(s) 90, 327, 540, 546
DdeI CTNAG 2 cut(s) 755, 789
Eam1104I CTCTTC 2 cut(s) 272, 627
EarI CTCTTC 2 cut(s) 272, 627
Ecl136II GAGCTC 1 cut(s) 754
Eco130I CCWWGG 4 cut(s) 306, 488, 582, 655
Eco24I GRGCYC 2 cut(s) 262, 756
Eco47I GGWCC 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 754
Eco88I CYCGRG 1 cut(s) 261
EcoICRI GAGCTC 1 cut(s) 754
EcoRII CCWGG 3 cut(s) 92, 322, 606
EcoT14I CCWWGG 4 cut(s) 306, 488, 582, 655
EcoT38I GRGCYC 2 cut(s) 262, 756
ErhI CCWWGG 4 cut(s) 306, 488, 582, 655
FaeI CATG 4 cut(s) 310, 492, 586, 640
FaiI YATR 7 cut(s) 210, 308, 389, 490, 584, 638, 696
FatI CATG 4 cut(s) 306, 488, 582, 636
FblI GTMKAC 2 cut(s) 407, 495
Fnu4HI GCNGC 4 cut(s) 51, 294, 691, 777
FokI GGATG 2 cut(s) 44, 638
FriOI GRGCYC 2 cut(s) 262, 756
Fsp4HI GCNGC 4 cut(s) 51, 294, 691, 777
FspBI CTAG 4 cut(s) 315, 411, 537, 543
GluI GCNGC 4 cut(s) 51, 294, 691, 777
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 2 cut(s) 93, 179
HaeIII GGCC 2 cut(s) 454, 487
HapII CCGG 4 cut(s) 54, 642, 708, 771
Hin1II CATG 4 cut(s) 310, 492, 586, 640
HincII GTYRAC 2 cut(s) 496, 508
HindII GTYRAC 2 cut(s) 496, 508
HinfI GANTC 2 cut(s) 100, 123
HpaII CCGG 4 cut(s) 54, 642, 708, 771
HphI GGTGA 3 cut(s) 516, 596, 656
Hpy166II GTNNAC 4 cut(s) 360, 408, 496, 508
Hpy188I TCNGA 2 cut(s) 679, 718
Hpy188III TCNNGA 4 cut(s) 110, 182, 247, 263
Hpy8I GTNNAC 4 cut(s) 360, 408, 496, 508
Hpy99I CGWCG 1 cut(s) 731
HpyAV CCTTC 1 cut(s) 538
HpyCH4III ACNGT 1 cut(s) 731
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 2 cut(s) 285, 501
HpyF10VI GCNNNNNNNGC 3 cut(s) 696, 720, 785
HpyF3I CTNAG 2 cut(s) 755, 789
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 4 cut(s) 310, 492, 586, 640
LmnI GCTCC 2 cut(s) 4, 740
Lsp1109I GCAGC 3 cut(s) 62, 280, 763
MabI ACCWGGT 1 cut(s) 92
MaeI CTAG 4 cut(s) 315, 411, 537, 543
MaeII ACGT 1 cut(s) 252
MboII GAAGA 4 cut(s) 289, 581, 611, 614
MflI RGATCY 6 cut(s) 133, 186, 335, 383, 431, 464
MhlI GDGCHC 5 cut(s) 164, 177, 262, 306, 756
MluCI AATT 4 cut(s) 349, 397, 445, 478
MroXI GAANNNNTTC 2 cut(s) 316, 621
MseI TTAA 2 cut(s) 156, 396
MspA1I CMGCKG 2 cut(s) 199, 458
MspI CCGG 4 cut(s) 54, 642, 708, 771
MspR9I CCNGG 6 cut(s) 55, 94, 324, 608, 642, 772
MvaI CCWGG 3 cut(s) 94, 324, 608
MwoI GCNNNNNNNGC 3 cut(s) 696, 720, 785
NciI CCSGG 3 cut(s) 55, 642, 772
NcoI CCATGG 3 cut(s) 306, 488, 582
NlaIII CATG 4 cut(s) 310, 492, 586, 640
NlaIV GGNNCC 6 cut(s) 337, 358, 385, 433, 466, 736
PaeR7I CTCGAG 1 cut(s) 261
PdmI GAANNNNTTC 2 cut(s) 316, 621
PfeI GAWTC 2 cut(s) 100, 123
PflMI CCANNNNNTGG 1 cut(s) 461
PkrI GCNGC 4 cut(s) 52, 295, 692, 778
PshBI ATTAAT 1 cut(s) 396
Psp124BI GAGCTC 1 cut(s) 756
Psp6I CCWGG 3 cut(s) 92, 322, 606
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 3 cut(s) 92, 322, 606
PspN4I GGNNCC 6 cut(s) 337, 358, 385, 433, 466, 736
PspPI GGNCC 3 cut(s) 357, 452, 485
PstI CTGCAG 1 cut(s) 503
PsuI RGATCY 6 cut(s) 133, 186, 335, 383, 431, 464
PvuII CAGCTG 2 cut(s) 199, 458
RsaI GTAC 4 cut(s) 91, 328, 541, 547
RsaNI GTAC 4 cut(s) 90, 327, 540, 546
SacI GAGCTC 1 cut(s) 756
SalI GTCGAC 1 cut(s) 494
SaqAI TTAA 2 cut(s) 156, 396
SatI GCNGC 4 cut(s) 51, 294, 691, 777
Sau96I GGNCC 3 cut(s) 357, 452, 485
ScaI AGTACT 2 cut(s) 541, 547
ScrFI CCNGG 6 cut(s) 55, 94, 324, 608, 642, 772
SduI GDGCHC 5 cut(s) 164, 177, 262, 306, 756
SexAI ACCWGGT 1 cut(s) 92
SfcI CTRYAG 2 cut(s) 168, 499
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 357
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 4 cut(s) 128, 261, 297, 749
SmoI CTYRAG 4 cut(s) 128, 261, 297, 749
SpeI ACTAGT 4 cut(s) 314, 410, 536, 542
Sse9I AATT 4 cut(s) 349, 397, 445, 478
SsiI CCGC 1 cut(s) 690
SspMI CTAG 4 cut(s) 315, 411, 537, 543
SstI GAGCTC 1 cut(s) 756
StyD4I CCNGG 6 cut(s) 53, 92, 322, 606, 640, 770
StyI CCWWGG 4 cut(s) 306, 488, 582, 655
TaaI ACNGT 1 cut(s) 731
TaiI ACGT 1 cut(s) 255
TaqI TCGA 4 cut(s) 27, 262, 438, 495
TasI AATT 4 cut(s) 349, 397, 445, 478
TatI WGTACW 2 cut(s) 539, 545
TauI GCSGC 1 cut(s) 693
TfiI GAWTC 2 cut(s) 100, 123
Tru1I TTAA 2 cut(s) 156, 396
Tru9I TTAA 2 cut(s) 156, 396
TscAI CASTG 2 cut(s) 367, 508
TseI GCWGC 3 cut(s) 50, 293, 776
TspDTI ATGAA 2 cut(s) 290, 582
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 2 cut(s) 367, 508
Van91I CCANNNNNTGG 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 357
VspI ATTAAT 1 cut(s) 396
XhoI CTCGAG 1 cut(s) 261
XmiI GTMKAC 2 cut(s) 407, 495
XmnI GAANNNNTTC 2 cut(s) 316, 621
XspI CTAG 4 cut(s) 315, 411, 537, 543
ZrmI AGTACT 2 cut(s) 541, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.