Rh6AG245500

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0019028
Physical Location & Seq
Reverse (-)
43403447 .. 43407057
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG245500.1

Sequence Viewer

Length: 897 bp
ATGTCACCTGCACTGGCCTCAACCTACCTGGCATTTCATCCTTTGAACAGCAACATCATAACGATTGTAACAGAAAATTCAATCATTCACATTTACTATCTTAGAGTTGATGAGGTGATCAGACCAAAATTCAAGTGCCATCAGAAACACATTCCTGGATTTGCATTTTCTGTCAACCTTAAAGTCATGGTTTCATCAGAGGCTGATGCTCAAGCTCAACTACGCTTTTGGAACATGGATACCTGGGATAAGAGGAAATTAGTTAGGATTCAACTGCCAACTGGAAAGGCGCCTACTGGTGATACTCAAGTGCAATTATACTCTGATCAAATCCTGTTGTTGGTATTGGTTAAGACTCAACCAGCACTATATGATGGTGCCAAAACAGAATGCATTCAGCAGTGGATGCCACGAGATGTGCTGCCTGCACCGATAACATGTGCAGCATATTCCCCGAATAATCAACTCTATGGGGCTTTATCTGATAGCAATGTTGCAGTATTTGATACTGACAGTCTGAAACTTAGATGTCGTATAGCCATGTCAGTGTACTTTGTAGAAGCATCCCGCAACAGGTTCAAGGTTCCTCAACCTGGTTCAAACGCTGGGATTGGTGCTGTGCTAGTGACTGCTCAGGAAGTGGAAGCTCATAGTGAAAAGGGTCTGCTTGTTGGAGCGGTTGCAGAGAGTGATGCTACTGAGGACCCCATTTTTCCTGGAAAGCGCATGAGCAAGATGAAGGACGCAGCTGGGGTATCTCTCAGAGTATTTTTGGCGACACCAGGAGGAAGATTTTTACTGTTGCTGAGACTGAGCTCAACAAAACTGCTGCTGCTGCTGCTGATCTCCAAGGCAGTTGAATACACAACACTGGTGATTTCACTACTGCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

298

Amino Acids

32.86

Weight (kDa)

9.08

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019028)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0280621
rosa_laevigata RLG00000013043
rosa_roxburghii Rroxscaffold_7G00187870
rosa_rugosa Rorug06G0132900
rosa_samantha Rh6AG245500 Rh6BG247800 Rh6CG249300 Rh6DG240500
rosa_wichuraiana Rw6G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 16
Acc36I ACCTGC 1 cut(s) 16
AccB1I GGYRCC 2 cut(s) 289, 377
AccBSI CCGCTC 1 cut(s) 677
AciI CCGC 2 cut(s) 568, 677
AcsI RAATTY 2 cut(s) 76, 128
AcyI GRCGYC 1 cut(s) 290
AfaI GTAC 1 cut(s) 551
AfiI CCNNNNNNNGG 3 cut(s) 340, 573, 593
AflIII ACRYGT 1 cut(s) 437
AgsI TTSAA 7 cut(s) 46, 81, 133, 272, 580, 600, 860
AjnI CCWGG 6 cut(s) 27, 154, 242, 592, 715, 781
AluBI AGCT 4 cut(s) 215, 647, 749, 816
AluI AGCT 4 cut(s) 215, 647, 749, 816
Alw21I GWGCWC 1 cut(s) 818
Alw26I GTCTC 1 cut(s) 802
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 7 cut(s) 421, 443, 746, 829, 832, 835, 838
ApoI RAATTY 2 cut(s) 76, 128
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 2 cut(s) 292, 726
AspS9I GGNCC 1 cut(s) 703
AsuHPI GGTGA 3 cut(s) 127, 311, 886
AvaII GGWCC 1 cut(s) 703
BaeI ACNNNNGTAYC 2 cut(s) 294, 327
BanI GGYRCC 2 cut(s) 289, 377
BanII GRGCYC 1 cut(s) 818
BauI CACGAG 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 818
BbvI GCAGC 7 cut(s) 408, 455, 758, 816, 819, 822, 825
BccI CCATC 2 cut(s) 147, 368
BciT130I CCWGG 6 cut(s) 29, 156, 244, 594, 717, 783
BciVI GTATCC 1 cut(s) 232
BclI TGATCA 2 cut(s) 117, 325
BcoDI GTCTC 1 cut(s) 802
BfaI CTAG 1 cut(s) 623
BfoI RGCGCY 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 16
BfuI GTATCC 1 cut(s) 232
BisI GCNGC 7 cut(s) 422, 444, 747, 830, 833, 836, 839
BlsI GCNGC 7 cut(s) 423, 445, 748, 831, 834, 837, 840
Bme1390I CCNGG 6 cut(s) 29, 156, 244, 594, 717, 783
Bme18I GGWCC 1 cut(s) 703
BmgT120I GGNCC 1 cut(s) 703
BmiI GGNNCC 4 cut(s) 291, 379, 585, 705
BmrFI CCNGG 6 cut(s) 29, 156, 244, 594, 717, 783
BmsI GCATC 4 cut(s) 196, 396, 572, 682
Bpu10I CCTNAGC 1 cut(s) 633
BpuEI CTTGAG 2 cut(s) 195, 291
BsaHI GRCGYC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 243, 849
Bsc4I CCNNNNNNNGG 3 cut(s) 340, 573, 593
Bse1I ACTGG 4 cut(s) 18, 286, 301, 876
Bse3DI GCAATG 1 cut(s) 496
BseBI CCWGG 6 cut(s) 29, 156, 244, 594, 717, 783
BseDI CCNNGG 2 cut(s) 243, 849
BseGI GGATG 3 cut(s) 37, 411, 563
BseLI CCNNNNNNNGG 3 cut(s) 340, 573, 593
BseMI GCAATG 1 cut(s) 496
BseMII CTCAG 5 cut(s) 647, 690, 775, 797, 803
BseNI ACTGG 4 cut(s) 18, 286, 301, 876
BseXI GCAGC 7 cut(s) 408, 455, 758, 816, 819, 822, 825
BseYI CCCAGC 2 cut(s) 605, 749
BsgI GTGCAG 2 cut(s) 411, 462
BshFI GGCC 1 cut(s) 17
BshNI GGYRCC 2 cut(s) 289, 377
BsiHKAI GWGCWC 1 cut(s) 818
BslI CCNNNNNNNGG 3 cut(s) 340, 573, 593
BsmAI GTCTC 1 cut(s) 802
BsmI GAATGC 2 cut(s) 393, 395
BsnI GGCC 1 cut(s) 17
Bsp1286I GDGCHC 1 cut(s) 818
Bsp143I GATC 3 cut(s) 117, 325, 843
BspACI CCGC 2 cut(s) 568, 677
BspANI GGCC 1 cut(s) 17
BspCNI CTCAG 5 cut(s) 646, 691, 774, 798, 804
BspLI GGNNCC 4 cut(s) 291, 379, 585, 705
BspMI ACCTGC 1 cut(s) 16
BspT107I GGYRCC 2 cut(s) 289, 377
BsrBI CCGCTC 1 cut(s) 677
BsrDI GCAATG 1 cut(s) 496
BsrI ACTGG 4 cut(s) 18, 286, 301, 876
BssECI CCNNGG 2 cut(s) 243, 849
BssMI GATC 3 cut(s) 117, 325, 843
BssNI GRCGYC 1 cut(s) 290
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 1 cut(s) 849
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 6 cut(s) 29, 156, 244, 594, 717, 783
Bst4CI ACNGT 2 cut(s) 515, 801
BstACI GRCGYC 1 cut(s) 290
BstAPI GCANNNNNTGC 1 cut(s) 406
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 7 cut(s) 101, 524, 633, 699, 761, 806, 812
BstF5I GGATG 3 cut(s) 37, 411, 563
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 2 cut(s) 292, 726
BstKTI GATC 3 cut(s) 120, 328, 846
BstMAI GTCTC 1 cut(s) 802
BstMBI GATC 3 cut(s) 117, 325, 843
BstMWI GCNNNNNNNGC 3 cut(s) 406, 835, 838
BstNI CCWGG 6 cut(s) 29, 156, 244, 594, 717, 783
BstNSI RCATGY 1 cut(s) 441
BstSCI CCNGG 6 cut(s) 27, 154, 242, 592, 715, 781
BstV1I GCAGC 7 cut(s) 408, 455, 758, 816, 819, 822, 825
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 1 cut(s) 17
BtsCI GGATG 3 cut(s) 37, 411, 563
BtsI GCAGTG 1 cut(s) 407
BtsIMutI CAGTG 4 cut(s) 11, 407, 552, 869
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 203
CfoI GCGC 2 cut(s) 292, 726
Cfr13I GGNCC 1 cut(s) 703
CseI GACGC 1 cut(s) 752
CsiI ACCWGGT 1 cut(s) 592
Csp6I GTAC 1 cut(s) 550
CviAII CATG 6 cut(s) 187, 235, 438, 541, 727, 894
CviJI RGCY 8 cut(s) 17, 203, 215, 476, 539, 647, 749, 816
CviKI_1 RGCY 8 cut(s) 17, 203, 215, 476, 539, 647, 749, 816
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 7 cut(s) 101, 524, 633, 699, 761, 806, 812
DinI GGCGCC 1 cut(s) 291
DpnI GATC 3 cut(s) 119, 327, 845
DpnII GATC 3 cut(s) 117, 325, 843
Ecl136II GAGCTC 1 cut(s) 816
Eco130I CCWWGG 1 cut(s) 849
Eco24I GRGCYC 1 cut(s) 818
Eco47I GGWCC 1 cut(s) 703
Eco53kI GAGCTC 1 cut(s) 816
EcoICRI GAGCTC 1 cut(s) 816
EcoO109I RGGNCCY 1 cut(s) 703
EcoRII CCWGG 6 cut(s) 27, 154, 242, 592, 715, 781
EcoT14I CCWWGG 1 cut(s) 849
EcoT22I ATGCAT 1 cut(s) 395
EcoT38I GRGCYC 1 cut(s) 818
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 849
FaeI CATG 6 cut(s) 190, 238, 441, 544, 730, 897
FatI CATG 6 cut(s) 186, 234, 437, 540, 726, 893
FauI CCCGC 1 cut(s) 575
FbaI TGATCA 2 cut(s) 117, 325
Fnu4HI GCNGC 7 cut(s) 422, 444, 747, 830, 833, 836, 839
FokI GGATG 3 cut(s) 24, 418, 550
FriOI GRGCYC 1 cut(s) 818
Fsp4HI GCNGC 7 cut(s) 422, 444, 747, 830, 833, 836, 839
FspBI CTAG 1 cut(s) 623
GlaI GCGC 2 cut(s) 291, 725
GluI GCNGC 7 cut(s) 422, 444, 747, 830, 833, 836, 839
GsaI CCCAGC 2 cut(s) 609, 753
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 1 cut(s) 17
HgaI GACGC 1 cut(s) 752
HhaI GCGC 2 cut(s) 292, 726
Hin1I GRCGYC 1 cut(s) 290
Hin1II CATG 6 cut(s) 190, 238, 441, 544, 730, 897
Hin6I GCGC 2 cut(s) 290, 724
HinP1I GCGC 2 cut(s) 290, 724
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 2 cut(s) 268, 355
HphI GGTGA 3 cut(s) 127, 311, 886
Hpy166II GTNNAC 2 cut(s) 175, 550
Hpy188I TCNGA 7 cut(s) 122, 144, 199, 325, 484, 519, 764
Hpy188III TCNNGA 1 cut(s) 635
Hpy8I GTNNAC 2 cut(s) 175, 550
HpyAV CCTTC 1 cut(s) 733
HpyCH4III ACNGT 2 cut(s) 515, 801
HpyCH4V TGCA 8 cut(s) 11, 164, 313, 393, 428, 443, 497, 683
HpyF10VI GCNNNNNNNGC 3 cut(s) 406, 835, 838
HpyF3I CTNAG 7 cut(s) 101, 524, 633, 699, 761, 806, 812
Hsp92I GRCGYC 1 cut(s) 290
Hsp92II CATG 6 cut(s) 190, 238, 441, 544, 730, 897
HspAI GCGC 2 cut(s) 290, 724
KasI GGCGCC 1 cut(s) 289
Ksp22I TGATCA 2 cut(s) 117, 325
Kzo9I GATC 3 cut(s) 117, 325, 843
LmnI GCTCC 1 cut(s) 674
Lsp1109I GCAGC 7 cut(s) 408, 455, 758, 816, 819, 822, 825
LweI GCATC 4 cut(s) 196, 396, 572, 682
MabI ACCWGGT 1 cut(s) 592
MaeI CTAG 1 cut(s) 623
MaeIII GTNAC 3 cut(s) 3, 67, 625
MalI GATC 3 cut(s) 119, 327, 845
MbiI CCGCTC 1 cut(s) 677
MboI GATC 3 cut(s) 117, 325, 843
MboII GAAGA 1 cut(s) 801
MhlI GDGCHC 1 cut(s) 818
MluCI AATT 4 cut(s) 76, 128, 257, 314
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 1 cut(s) 349
MmeI TCCRAC 1 cut(s) 652
MnlI CCTC 7 cut(s) 28, 106, 193, 246, 597, 694, 779
Mph1103I ATGCAT 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 393
MseI TTAA 2 cut(s) 180, 351
MslI CAYNNNNRTG 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 749
MspR9I CCNGG 6 cut(s) 29, 156, 244, 594, 717, 783
Mva1269I GAATGC 2 cut(s) 393, 395
MvaI CCWGG 6 cut(s) 29, 156, 244, 594, 717, 783
MwoI GCNNNNNNNGC 3 cut(s) 406, 835, 838
NarI GGCGCC 1 cut(s) 290
NdeII GATC 3 cut(s) 117, 325, 843
NlaIII CATG 6 cut(s) 190, 238, 441, 544, 730, 897
NlaIV GGNNCC 4 cut(s) 291, 379, 585, 705
NmuCI GTSAC 2 cut(s) 3, 625
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 1 cut(s) 441
PaqCI CACCTGC 1 cut(s) 16
PciI ACATGT 1 cut(s) 437
PctI GAATGC 2 cut(s) 393, 395
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 1 cut(s) 268
PfoI TCCNGGA 2 cut(s) 154, 715
PkrI GCNGC 7 cut(s) 423, 445, 748, 831, 834, 837, 840
PleI GAGTC 1 cut(s) 349
PluTI GGCGCC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 349
PpuMI RGGWCCY 1 cut(s) 703
PscI ACATGT 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 818
Psp5II RGGWCCY 1 cut(s) 703
Psp6I CCWGG 6 cut(s) 27, 154, 242, 592, 715, 781
PspFI CCCAGC 2 cut(s) 605, 749
PspGI CCWGG 6 cut(s) 27, 154, 242, 592, 715, 781
PspN4I GGNNCC 4 cut(s) 291, 379, 585, 705
PspPI GGNCC 1 cut(s) 703
PspPPI RGGWCCY 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 203
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
RseI CAYNNNNRTG 1 cut(s) 545
SacI GAGCTC 1 cut(s) 818
SaqAI TTAA 2 cut(s) 180, 351
SatI GCNGC 7 cut(s) 422, 444, 747, 830, 833, 836, 839
Sau3AI GATC 3 cut(s) 117, 325, 843
Sau96I GGNCC 1 cut(s) 703
SchI GAGTC 1 cut(s) 349
ScrFI CCNGG 6 cut(s) 29, 156, 244, 594, 717, 783
SduI GDGCHC 1 cut(s) 818
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 4 cut(s) 196, 396, 572, 682
SfoI GGCGCC 1 cut(s) 291
SinI GGWCC 1 cut(s) 703
SmiMI CAYNNNNRTG 1 cut(s) 545
SmlI CTYRAG 2 cut(s) 210, 306
SmoI CTYRAG 2 cut(s) 210, 306
Sse9I AATT 4 cut(s) 76, 128, 257, 314
SsiI CCGC 2 cut(s) 568, 677
SspDI GGCGCC 1 cut(s) 289
SspMI CTAG 1 cut(s) 623
SstI GAGCTC 1 cut(s) 818
StyD4I CCNGG 6 cut(s) 27, 154, 242, 592, 715, 781
StyI CCWWGG 1 cut(s) 849
TaaI ACNGT 2 cut(s) 515, 801
TasI AATT 4 cut(s) 76, 128, 257, 314
TatI WGTACW 1 cut(s) 549
TfiI GAWTC 1 cut(s) 268
Tru1I TTAA 2 cut(s) 180, 351
Tru9I TTAA 2 cut(s) 180, 351
TscAI CASTG 4 cut(s) 18, 407, 552, 876
TseFI GTSAC 2 cut(s) 3, 625
TseI GCWGC 7 cut(s) 421, 443, 746, 829, 832, 835, 838
Tsp45I GTSAC 2 cut(s) 3, 625
TspDTI ATGAA 3 cut(s) 26, 183, 752
TspRI CASTG 4 cut(s) 18, 407, 552, 876
VpaK11BI GGWCC 1 cut(s) 703
XapI RAATTY 2 cut(s) 76, 128
XceI RCATGY 1 cut(s) 441
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 1 cut(s) 623
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.