Rh6DG240500

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0019028
Physical Location & Seq
Reverse (-)
42751526 .. 42753047
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG240500.1

Sequence Viewer

Length: 609 bp
ATGGATACCTGGGATAAGAGGAAATTAGTTAGGATTCAACTGCCAACTGGAAAGGCGCCTACTGGTGATACTCAAGTGCAATTCTACTCTGATCAAATCCTGTTGTTGGTATTGGTTAAGACTCAACCAGCACTATATGATGGTGCCAAAACAGAATGCATTCAGCAGTGGATGCCACGAGATGTGCTGCCTGCACCGATAACATGTGCAGCATATTCCCCGAATAATCAACTCTATGGGGCTTTATCTGATAGCAATGTTGCAGTATTTGATACTGACAGTCTGAAACTTAGATGTCGTATAGCCATGTCAGTGTACTTTGTAGAAGCATCCCGCAACAGGTTCAAGGTTCCTCAACCTGGTTCAAACGCTGGGATTGGTGCTGTGCTAGTGACTGCTCAGGAAGTGGAAGCTCATAGTGAAAAGGGTCTGCTTGTTGGAGCGGTTGCAGAGAGTGATGCTACTGAGGACCCCATTTTTCCTGGAAAGCGCATGAGCAAGATGAAGGACGCAGCTGGGGTATCTCTCAGAGTATTTTTGGCGACACCAGGGGGAAGATTTTTACTGTTGCTGAGACTGAGCTCAACAAAACTGCTGCTGCTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

202

Amino Acids

22.02

Weight (kDa)

8.9

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019028)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0280621
rosa_laevigata RLG00000013043
rosa_roxburghii Rroxscaffold_7G00187870
rosa_rugosa Rorug06G0132900
rosa_samantha Rh6AG245500 Rh6BG247800 Rh6CG249300 Rh6DG240500
rosa_wichuraiana Rw6G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 55, 143
AccBSI CCGCTC 1 cut(s) 443
AciI CCGC 2 cut(s) 334, 443
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 3 cut(s) 106, 339, 359
AflIII ACRYGT 1 cut(s) 203
AgsI TTSAA 3 cut(s) 38, 346, 366
AjnI CCWGG 4 cut(s) 8, 358, 481, 547
AluBI AGCT 3 cut(s) 413, 515, 582
AluI AGCT 3 cut(s) 413, 515, 582
Alw21I GWGCWC 1 cut(s) 584
Alw26I GTCTC 1 cut(s) 568
ApeKI GCWGC 5 cut(s) 187, 209, 512, 595, 598
Asp700I GAANNNNTTC 1 cut(s) 159
AspLEI GCGC 2 cut(s) 58, 492
AspS9I GGNCC 1 cut(s) 469
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 469
BaeI ACNNNNGTAYC 2 cut(s) 60, 93
BanI GGYRCC 2 cut(s) 55, 143
BanII GRGCYC 1 cut(s) 584
BauI CACGAG 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 584
BbvI GCAGC 5 cut(s) 174, 221, 524, 582, 585
BccI CCATC 1 cut(s) 134
BciT130I CCWGG 4 cut(s) 10, 360, 483, 549
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 1 cut(s) 389
BfoI RGCGCY 1 cut(s) 59
BisI GCNGC 5 cut(s) 188, 210, 513, 596, 599
BlsI GCNGC 5 cut(s) 189, 211, 514, 597, 600
Bme1390I CCNGG 4 cut(s) 10, 360, 483, 549
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 4 cut(s) 57, 145, 351, 471
BmrFI CCNGG 4 cut(s) 10, 360, 483, 549
BmsI GCATC 3 cut(s) 162, 338, 448
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 57
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 9, 548
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 339, 359
Bse1I ACTGG 2 cut(s) 52, 67
Bse3DI GCAATG 1 cut(s) 262
BseBI CCWGG 4 cut(s) 10, 360, 483, 549
BseDI CCNNGG 2 cut(s) 9, 548
BseGI GGATG 2 cut(s) 177, 329
BseLI CCNNNNNNNGG 3 cut(s) 106, 339, 359
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 5 cut(s) 413, 456, 541, 563, 569
BseNI ACTGG 2 cut(s) 52, 67
BseXI GCAGC 5 cut(s) 174, 221, 524, 582, 585
BseYI CCCAGC 2 cut(s) 371, 515
BsgI GTGCAG 2 cut(s) 177, 228
BshNI GGYRCC 2 cut(s) 55, 143
BsiHKAI GWGCWC 1 cut(s) 584
BslI CCNNNNNNNGG 3 cut(s) 106, 339, 359
BsmAI GTCTC 1 cut(s) 568
BsmI GAATGC 2 cut(s) 159, 161
Bsp1286I GDGCHC 1 cut(s) 584
Bsp143I GATC 1 cut(s) 91
BspACI CCGC 2 cut(s) 334, 443
BspCNI CTCAG 5 cut(s) 412, 457, 540, 564, 570
BspLI GGNNCC 4 cut(s) 57, 145, 351, 471
BspT107I GGYRCC 2 cut(s) 55, 143
BsrBI CCGCTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 2 cut(s) 52, 67
BssECI CCNNGG 2 cut(s) 9, 548
BssMI GATC 1 cut(s) 91
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 177
Bst2BI CACGAG 1 cut(s) 177
Bst2UI CCWGG 4 cut(s) 10, 360, 483, 549
Bst4CI ACNGT 2 cut(s) 281, 567
BstACI GRCGYC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 172
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 6 cut(s) 290, 399, 465, 527, 572, 578
BstF5I GGATG 2 cut(s) 177, 329
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 2 cut(s) 58, 492
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 4 cut(s) 10, 360, 483, 549
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 4 cut(s) 8, 358, 481, 547
BstV1I GCAGC 5 cut(s) 174, 221, 524, 582, 585
BtsCI GGATG 2 cut(s) 177, 329
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 2 cut(s) 173, 318
Cac8I GCNNGC 1 cut(s) 192
CfoI GCGC 2 cut(s) 58, 492
Cfr13I GGNCC 1 cut(s) 469
CseI GACGC 1 cut(s) 518
CsiI ACCWGGT 1 cut(s) 358
Csp6I GTAC 1 cut(s) 316
CviAII CATG 3 cut(s) 204, 307, 493
CviJI RGCY 5 cut(s) 242, 305, 413, 515, 582
CviKI_1 RGCY 5 cut(s) 242, 305, 413, 515, 582
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 6 cut(s) 290, 399, 465, 527, 572, 578
DinI GGCGCC 1 cut(s) 57
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 582
Eco24I GRGCYC 1 cut(s) 584
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 582
EcoICRI GAGCTC 1 cut(s) 582
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 4 cut(s) 8, 358, 481, 547
EcoT22I ATGCAT 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 584
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
FaeI CATG 3 cut(s) 207, 310, 496
FaiI YATR 9 cut(s) 136, 138, 205, 214, 237, 302, 308, 417, 494
FatI CATG 3 cut(s) 203, 306, 492
FauI CCCGC 1 cut(s) 341
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 5 cut(s) 188, 210, 513, 596, 599
FokI GGATG 2 cut(s) 184, 316
FriOI GRGCYC 1 cut(s) 584
Fsp4HI GCNGC 5 cut(s) 188, 210, 513, 596, 599
FspBI CTAG 1 cut(s) 389
GlaI GCGC 2 cut(s) 57, 491
GluI GCNGC 5 cut(s) 188, 210, 513, 596, 599
GsaI CCCAGC 2 cut(s) 375, 519
HaeII RGCGCY 1 cut(s) 59
HgaI GACGC 1 cut(s) 518
HhaI GCGC 2 cut(s) 58, 492
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 3 cut(s) 207, 310, 496
Hin6I GCGC 2 cut(s) 56, 490
HinP1I GCGC 2 cut(s) 56, 490
HinfI GANTC 2 cut(s) 34, 121
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 4 cut(s) 91, 250, 285, 530
Hpy188III TCNNGA 1 cut(s) 401
Hpy8I GTNNAC 1 cut(s) 316
HpyAV CCTTC 1 cut(s) 499
HpyCH4III ACNGT 2 cut(s) 281, 567
HpyCH4V TGCA 6 cut(s) 79, 159, 194, 209, 263, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 6 cut(s) 290, 399, 465, 527, 572, 578
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 3 cut(s) 207, 310, 496
HspAI GCGC 2 cut(s) 56, 490
KasI GGCGCC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 1 cut(s) 91
LmnI GCTCC 1 cut(s) 440
Lsp1109I GCAGC 5 cut(s) 174, 221, 524, 582, 585
LweI GCATC 3 cut(s) 162, 338, 448
MabI ACCWGGT 1 cut(s) 358
MaeI CTAG 1 cut(s) 389
MaeIII GTNAC 1 cut(s) 391
MalI GATC 1 cut(s) 93
MbiI CCGCTC 1 cut(s) 443
MboI GATC 1 cut(s) 91
MboII GAAGA 1 cut(s) 567
MhlI GDGCHC 1 cut(s) 584
MluCI AATT 2 cut(s) 23, 80
Mly113I GGCGCC 1 cut(s) 56
MlyI GAGTC 1 cut(s) 115
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 3 cut(s) 12, 363, 460
Mph1103I ATGCAT 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 159
MseI TTAA 1 cut(s) 117
MslI CAYNNNNRTG 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 515
MspR9I CCNGG 4 cut(s) 10, 360, 483, 549
Mva1269I GAATGC 2 cut(s) 159, 161
MvaI CCWGG 4 cut(s) 10, 360, 483, 549
MwoI GCNNNNNNNGC 1 cut(s) 172
NarI GGCGCC 1 cut(s) 56
NdeII GATC 1 cut(s) 91
NlaIII CATG 3 cut(s) 207, 310, 496
NlaIV GGNNCC 4 cut(s) 57, 145, 351, 471
NmuCI GTSAC 1 cut(s) 391
NsiI ATGCAT 1 cut(s) 161
NspI RCATGY 1 cut(s) 207
PciI ACATGT 1 cut(s) 203
PctI GAATGC 2 cut(s) 159, 161
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 34
PfoI TCCNGGA 1 cut(s) 481
PkrI GCNGC 5 cut(s) 189, 211, 514, 597, 600
PleI GAGTC 1 cut(s) 115
PluTI GGCGCC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 115
PpuMI RGGWCCY 1 cut(s) 469
PscI ACATGT 1 cut(s) 203
Psp124BI GAGCTC 1 cut(s) 584
Psp5II RGGWCCY 1 cut(s) 469
Psp6I CCWGG 4 cut(s) 8, 358, 481, 547
PspFI CCCAGC 2 cut(s) 371, 515
PspGI CCWGG 4 cut(s) 8, 358, 481, 547
PspN4I GGNNCC 4 cut(s) 57, 145, 351, 471
PspPI GGNCC 1 cut(s) 469
PspPPI RGGWCCY 1 cut(s) 469
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 311
SacI GAGCTC 1 cut(s) 584
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 5 cut(s) 188, 210, 513, 596, 599
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 4 cut(s) 10, 360, 483, 549
SduI GDGCHC 1 cut(s) 584
SetI ASST 7 cut(s) 11, 344, 351, 361, 415, 517, 584
SexAI ACCWGGT 1 cut(s) 358
SfaNI GCATC 3 cut(s) 162, 338, 448
SfoI GGCGCC 1 cut(s) 57
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 2 cut(s) 23, 80
SsiI CCGC 2 cut(s) 334, 443
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 1 cut(s) 389
SstI GAGCTC 1 cut(s) 584
StyD4I CCNGG 4 cut(s) 8, 358, 481, 547
TaaI ACNGT 2 cut(s) 281, 567
TasI AATT 2 cut(s) 23, 80
TatI WGTACW 1 cut(s) 315
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 2 cut(s) 173, 318
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 5 cut(s) 187, 209, 512, 595, 598
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 1 cut(s) 518
TspRI CASTG 2 cut(s) 173, 318
VpaK11BI GGWCC 1 cut(s) 469
XceI RCATGY 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 1 cut(s) 389
Zsp2I ATGCAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.