Rh6CG249300

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0019028
Physical Location & Seq
Reverse (-)
41930780 .. 41933724
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG249300.1

Sequence Viewer

Length: 849 bp
ATGGAAAGAAGACCAGTAGCTGATGTACCTGATGAAAATAAACTTTGGCAACTGGTAGATATTGTTGATCCTCTTCACTGTCTAGTGGTTACGATGCCGCCGTATAGTAAAGTACCTGCTAATAAGGTAATAACAACTTTCATGTCACCTGCACTGGCCTCAACCTACCTGGCATTTCATCCTTTGAACAGCAACATCATAACGATTGTAACAGATAATTCAATCATTCACATTTACTATCTTAGAGTTGATGAGTGCCATCAGAAACACATTCCTGGATTTGCATTTTCTGTCAACCTTAAAGTCATGGTTTCATCAGAGGCTGATGCTCAAGCTCAACTACGCTTTTGGAACATGGATACCTGGGATAAGGGGAAATTAGTTAGGATTCAACTGCCAACTGGAAAGGCGCCTACTGGTGATACTCAAGTGCAATTCTACTCTGATCAAATCCTGTTGTTGGTATTGGTTAAGACTCAACCAGCACTATATGATGGTGCCAAAACAGAATGCATTCAACAGGTTCCTCAACCTGGTTCAAACGCTGGGATTGGTGCTGTGCTAGTGACTGCTCAGGAAGTGGAAGCTTATAGTGAAAAGGGTCTGCTTGTTGGAGCGGTTGCAGAGAGTGATGCTACCGAGGACCCCATTTTTCCTGGAGAGCGCGTGAGCAAGATGAAGGACGCAGCTGGGGTATCTCTCAGAGTATTTTTGGCGACACCAGGAGGAAGATTTTTACTGTTGCTGAGACTGAGCTCAACAAAACTGCTGCTGCTGCTGATCTCCAAGGCAGTTGAATACACAACACTTGTGATTTACACTACTGCAGCTTCCATGATCCATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

282

Amino Acids

30.97

Weight (kDa)

6.07

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019028)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0280621
rosa_laevigata RLG00000013043
rosa_roxburghii Rroxscaffold_7G00187870
rosa_rugosa Rorug06G0132900
rosa_samantha Rh6AG245500 Rh6BG247800 Rh6CG249300 Rh6DG240500
rosa_wichuraiana Rw6G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 157
Acc36I ACCTGC 2 cut(s) 124, 157
AccB1I GGYRCC 2 cut(s) 409, 497
AccBSI CCGCTC 1 cut(s) 617
AccII CGCG 1 cut(s) 666
AciI CCGC 2 cut(s) 98, 617
AclWI GGATC 2 cut(s) 62, 832
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 2 cut(s) 27, 114
AfiI CCNNNNNNNGG 2 cut(s) 460, 533
AgsI TTSAA 6 cut(s) 187, 222, 392, 518, 540, 797
AjnI CCWGG 6 cut(s) 168, 274, 362, 532, 655, 721
AluBI AGCT 6 cut(s) 20, 335, 587, 689, 756, 830
AluI AGCT 6 cut(s) 20, 335, 587, 689, 756, 830
Alw21I GWGCWC 1 cut(s) 758
Alw26I GTCTC 1 cut(s) 742
AlwI GGATC 2 cut(s) 62, 832
AlwNI CAGNNNCTG 2 cut(s) 20, 323
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 5 cut(s) 686, 769, 772, 775, 827
Asp700I GAANNNNTTC 1 cut(s) 513
AspLEI GCGC 2 cut(s) 412, 666
AspS9I GGNCC 1 cut(s) 643
AsuHPI GGTGA 2 cut(s) 138, 431
AvaII GGWCC 1 cut(s) 643
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BanI GGYRCC 2 cut(s) 409, 497
BanII GRGCYC 1 cut(s) 758
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 1 cut(s) 758
BbvI GCAGC 5 cut(s) 698, 756, 759, 762, 839
BccI CCATC 2 cut(s) 267, 488
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 6 cut(s) 170, 276, 364, 534, 657, 723
BciVI GTATCC 1 cut(s) 352
BclI TGATCA 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 742
BfaI CTAG 2 cut(s) 83, 563
BfmI CTRYAG 1 cut(s) 825
BfoI RGCGCY 1 cut(s) 413
BfuAI ACCTGC 2 cut(s) 124, 157
BfuI GTATCC 1 cut(s) 352
BisI GCNGC 6 cut(s) 98, 687, 770, 773, 776, 828
BlsI GCNGC 6 cut(s) 99, 688, 771, 774, 777, 829
Bme1390I CCNGG 6 cut(s) 170, 276, 364, 534, 657, 723
Bme18I GGWCC 1 cut(s) 643
BmgT120I GGNCC 1 cut(s) 643
BmiI GGNNCC 4 cut(s) 411, 499, 525, 645
BmrFI CCNGG 6 cut(s) 170, 276, 364, 534, 657, 723
BmsI GCATC 3 cut(s) 84, 316, 622
BpiI GAAGAC 1 cut(s) 16
BpmI CTGGAG 1 cut(s) 678
Bpu10I CCTNAGC 1 cut(s) 573
BpuEI CTTGAG 2 cut(s) 315, 411
BsaHI GRCGYC 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 363, 639, 786
BsaXI ACNNNNNCTCC 2 cut(s) 651, 681
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 533
Bse1I ACTGG 5 cut(s) 14, 57, 159, 406, 421
BseBI CCWGG 6 cut(s) 170, 276, 364, 534, 657, 723
BseDI CCNNGG 3 cut(s) 363, 639, 786
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 2 cut(s) 460, 533
BseMII CTCAG 4 cut(s) 587, 715, 737, 743
BseNI ACTGG 5 cut(s) 14, 57, 159, 406, 421
BseXI GCAGC 5 cut(s) 698, 756, 759, 762, 839
BseYI CCCAGC 2 cut(s) 545, 689
BsgI GTGCAG 1 cut(s) 135
Bsh1236I CGCG 1 cut(s) 666
BshFI GGCC 1 cut(s) 158
BshNI GGYRCC 2 cut(s) 409, 497
BsiHKAI GWGCWC 1 cut(s) 758
BslI CCNNNNNNNGG 2 cut(s) 460, 533
BsmAI GTCTC 1 cut(s) 742
BsmI GAATGC 2 cut(s) 513, 515
BsnI GGCC 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 758
Bsp143I GATC 4 cut(s) 67, 445, 780, 837
BspACI CCGC 2 cut(s) 98, 617
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 4 cut(s) 586, 714, 738, 744
BspFNI CGCG 1 cut(s) 666
BspLI GGNNCC 4 cut(s) 411, 499, 525, 645
BspMAI CTGCAG 1 cut(s) 829
BspMI ACCTGC 2 cut(s) 124, 157
BspPI GGATC 2 cut(s) 62, 832
BspT107I GGYRCC 2 cut(s) 409, 497
BsrBI CCGCTC 1 cut(s) 617
BsrI ACTGG 5 cut(s) 14, 57, 159, 406, 421
BssECI CCNNGG 3 cut(s) 363, 639, 786
BssMI GATC 4 cut(s) 67, 445, 780, 837
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 1 cut(s) 786
Bst2UI CCWGG 6 cut(s) 170, 276, 364, 534, 657, 723
Bst4CI ACNGT 2 cut(s) 80, 741
Bst6I CTCTTC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 410
BstDEI CTNAG 5 cut(s) 242, 573, 701, 746, 752
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 666
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 2 cut(s) 412, 666
BstKTI GATC 4 cut(s) 70, 448, 783, 840
BstMAI GTCTC 1 cut(s) 742
BstMBI GATC 4 cut(s) 67, 445, 780, 837
BstMWI GCNNNNNNNGC 1 cut(s) 775
BstNI CCWGG 6 cut(s) 170, 276, 364, 534, 657, 723
BstSCI CCNGG 6 cut(s) 168, 274, 362, 532, 655, 721
BstSFI CTRYAG 1 cut(s) 825
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 5 cut(s) 698, 756, 759, 762, 839
BstV2I GAAGAC 1 cut(s) 16
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 1 cut(s) 158
BtsCI GGATG 1 cut(s) 178
BtsIMutI CAGTG 2 cut(s) 76, 152
BveI ACCTGC 2 cut(s) 124, 157
CaiI CAGNNNCTG 2 cut(s) 20, 323
CfoI GCGC 2 cut(s) 412, 666
Cfr13I GGNCC 1 cut(s) 643
CseI GACGC 1 cut(s) 692
CsiI ACCWGGT 1 cut(s) 532
Csp6I GTAC 2 cut(s) 26, 113
CviAII CATG 4 cut(s) 142, 307, 355, 835
CviJI RGCY 8 cut(s) 20, 158, 323, 335, 587, 689, 756, 830
CviKI_1 RGCY 8 cut(s) 20, 158, 323, 335, 587, 689, 756, 830
CviQI GTAC 2 cut(s) 26, 113
DdeI CTNAG 5 cut(s) 242, 573, 701, 746, 752
DinI GGCGCC 1 cut(s) 411
DpnI GATC 4 cut(s) 69, 447, 782, 839
DpnII GATC 4 cut(s) 67, 445, 780, 837
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Ecl136II GAGCTC 1 cut(s) 756
Eco130I CCWWGG 1 cut(s) 786
Eco24I GRGCYC 1 cut(s) 758
Eco47I GGWCC 1 cut(s) 643
Eco53kI GAGCTC 1 cut(s) 756
EcoICRI GAGCTC 1 cut(s) 756
EcoO109I RGGNCCY 1 cut(s) 643
EcoRII CCWGG 6 cut(s) 168, 274, 362, 532, 655, 721
EcoT14I CCWWGG 1 cut(s) 786
EcoT22I ATGCAT 1 cut(s) 515
EcoT38I GRGCYC 1 cut(s) 758
EgeI GGCGCC 1 cut(s) 411
EheI GGCGCC 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 786
FaeI CATG 4 cut(s) 145, 310, 358, 838
FaiI YATR 9 cut(s) 105, 143, 200, 308, 356, 490, 492, 591, 836
FatI CATG 4 cut(s) 141, 306, 354, 834
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 6 cut(s) 98, 687, 770, 773, 776, 828
FokI GGATG 1 cut(s) 165
FriOI GRGCYC 1 cut(s) 758
Fsp4HI GCNGC 6 cut(s) 98, 687, 770, 773, 776, 828
FspBI CTAG 2 cut(s) 83, 563
GlaI GCGC 2 cut(s) 411, 665
GluI GCNGC 6 cut(s) 98, 687, 770, 773, 776, 828
GsaI CCCAGC 2 cut(s) 549, 693
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 1 cut(s) 158
HgaI GACGC 1 cut(s) 692
HhaI GCGC 2 cut(s) 412, 666
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 4 cut(s) 145, 310, 358, 838
Hin6I GCGC 2 cut(s) 410, 664
HinP1I GCGC 2 cut(s) 410, 664
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 2 cut(s) 388, 475
HphI GGTGA 2 cut(s) 138, 431
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 4 cut(s) 264, 319, 445, 704
Hpy188III TCNNGA 1 cut(s) 575
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 1 cut(s) 673
HpyCH4III ACNGT 2 cut(s) 80, 741
HpyCH4V TGCA 6 cut(s) 152, 284, 433, 513, 623, 827
HpyF10VI GCNNNNNNNGC 1 cut(s) 775
HpyF3I CTNAG 5 cut(s) 242, 573, 701, 746, 752
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 4 cut(s) 145, 310, 358, 838
HspAI GCGC 2 cut(s) 410, 664
KasI GGCGCC 1 cut(s) 409
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 4 cut(s) 67, 445, 780, 837
LmnI GCTCC 1 cut(s) 614
Lsp1109I GCAGC 5 cut(s) 698, 756, 759, 762, 839
LweI GCATC 3 cut(s) 84, 316, 622
MabI ACCWGGT 1 cut(s) 532
MaeI CTAG 2 cut(s) 83, 563
MaeIII GTNAC 4 cut(s) 88, 144, 208, 565
MalI GATC 4 cut(s) 69, 447, 782, 839
MbiI CCGCTC 1 cut(s) 617
MboI GATC 4 cut(s) 67, 445, 780, 837
MboII GAAGA 3 cut(s) 21, 65, 741
MhlI GDGCHC 1 cut(s) 758
MluCI AATT 3 cut(s) 217, 377, 434
Mly113I GGCGCC 1 cut(s) 410
MlyI GAGTC 1 cut(s) 469
MmeI TCCRAC 1 cut(s) 592
MnlI CCTC 6 cut(s) 81, 169, 313, 537, 634, 719
Mph1103I ATGCAT 1 cut(s) 515
MroXI GAANNNNTTC 1 cut(s) 513
MseI TTAA 2 cut(s) 300, 471
MspA1I CMGCKG 1 cut(s) 689
MspR9I CCNGG 6 cut(s) 170, 276, 364, 534, 657, 723
Mva1269I GAATGC 2 cut(s) 513, 515
MvaI CCWGG 6 cut(s) 170, 276, 364, 534, 657, 723
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 1 cut(s) 775
NarI GGCGCC 1 cut(s) 410
NdeII GATC 4 cut(s) 67, 445, 780, 837
NlaIII CATG 4 cut(s) 145, 310, 358, 838
NlaIV GGNNCC 4 cut(s) 411, 499, 525, 645
NmuCI GTSAC 2 cut(s) 144, 565
NsiI ATGCAT 1 cut(s) 515
PaqCI CACCTGC 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PctI GAATGC 2 cut(s) 513, 515
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 1 cut(s) 388
PfoI TCCNGGA 2 cut(s) 274, 655
PkrI GCNGC 6 cut(s) 99, 688, 771, 774, 777, 829
PleI GAGTC 1 cut(s) 469
PluTI GGCGCC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 469
PpuMI RGGWCCY 1 cut(s) 643
Psp124BI GAGCTC 1 cut(s) 758
Psp5II RGGWCCY 1 cut(s) 643
Psp6I CCWGG 6 cut(s) 168, 274, 362, 532, 655, 721
PspFI CCCAGC 2 cut(s) 545, 689
PspGI CCWGG 6 cut(s) 168, 274, 362, 532, 655, 721
PspN4I GGNNCC 4 cut(s) 411, 499, 525, 645
PspPI GGNCC 1 cut(s) 643
PspPPI RGGWCCY 1 cut(s) 643
PstI CTGCAG 1 cut(s) 829
PstNI CAGNNNCTG 2 cut(s) 20, 323
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 2 cut(s) 27, 114
RsaNI GTAC 2 cut(s) 26, 113
SacI GAGCTC 1 cut(s) 758
SaqAI TTAA 2 cut(s) 300, 471
SatI GCNGC 6 cut(s) 98, 687, 770, 773, 776, 828
Sau3AI GATC 4 cut(s) 67, 445, 780, 837
Sau96I GGNCC 1 cut(s) 643
SchI GAGTC 1 cut(s) 469
ScrFI CCNGG 6 cut(s) 170, 276, 364, 534, 657, 723
SduI GDGCHC 1 cut(s) 758
SexAI ACCWGGT 1 cut(s) 532
SfaNI GCATC 3 cut(s) 84, 316, 622
SfcI CTRYAG 1 cut(s) 825
SfoI GGCGCC 1 cut(s) 411
SinI GGWCC 1 cut(s) 643
SmlI CTYRAG 2 cut(s) 330, 426
SmoI CTYRAG 2 cut(s) 330, 426
Sse9I AATT 3 cut(s) 217, 377, 434
SsiI CCGC 2 cut(s) 98, 617
SspDI GGCGCC 1 cut(s) 409
SspMI CTAG 2 cut(s) 83, 563
SstI GAGCTC 1 cut(s) 758
StyD4I CCNGG 6 cut(s) 168, 274, 362, 532, 655, 721
StyI CCWWGG 1 cut(s) 786
TaaI ACNGT 2 cut(s) 80, 741
TasI AATT 3 cut(s) 217, 377, 434
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 1 cut(s) 388
Tru1I TTAA 2 cut(s) 300, 471
Tru9I TTAA 2 cut(s) 300, 471
TscAI CASTG 2 cut(s) 83, 159
TseFI GTSAC 2 cut(s) 144, 565
TseI GCWGC 5 cut(s) 686, 769, 772, 775, 827
Tsp45I GTSAC 2 cut(s) 144, 565
TspDTI ATGAA 5 cut(s) 48, 130, 167, 303, 692
TspRI CASTG 2 cut(s) 83, 159
VpaK11BI GGWCC 1 cut(s) 643
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 2 cut(s) 83, 563
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.