Rh6BG247800

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0019028
Physical Location & Seq
Reverse (-)
45923469 .. 45925310
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG247800.1

Sequence Viewer

Length: 546 bp
ATGGTTTCATCAGAGGCCGATGCTCAAGCTCAACTACGCTTTTGGAACATGGAAACCTGGGATAAGAGGAAATTAGTTAGGATTCAACTGCCAACTGGAAAGGCGCCTACTGGTGATACTCAAGTGCAATTCTATTCTGATCAAATCCTGTTGTTGGTATTGGTTAAGACTCAACCAGCACTATATGATGGTGACAAAACAGAATGCATTCAACAGTGGATGCCACAAGATGTGCTGCCTGCACCGATAACATGTGCAGCATATTCCCCGAATAATCAACTCTATGGGGCTTTATCTGATAGCAATGTTGGAGTATTTGATATTGACAGTCTGAAACTTAGATGTCGTATAGCCATGTCAGTGTACTTTGTCGAAGCATCCCGCAACAGGTTCAAGGTTCCTCAACCTGGTTCAAACGCTGGGATTGGTGCTGTGCTAGTGACTGCTCAGGAAGTGGAAGCTCATAGTGAAAAGTGTCTGCTTGTTGGAGCGGTTGCAGAGAGTACGATGCTACTGAGGACCCCATTTTTCCTGGAGAGCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

181

Amino Acids

20.0

Weight (kDa)

5.17

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019028)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0280621
rosa_laevigata RLG00000013043
rosa_roxburghii Rroxscaffold_7G00187870
rosa_rugosa Rorug06G0132900
rosa_samantha Rh6AG245500 Rh6BG247800 Rh6CG249300 Rh6DG240500
rosa_wichuraiana Rw6G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 103
AccBSI CCGCTC 1 cut(s) 491
AciI CCGC 2 cut(s) 382, 491
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 2 cut(s) 365, 505
AfiI CCNNNNNNNGG 3 cut(s) 154, 387, 407
AflIII ACRYGT 1 cut(s) 251
AgsI TTSAA 4 cut(s) 86, 212, 394, 414
AjnI CCWGG 3 cut(s) 56, 406, 531
AluBI AGCT 2 cut(s) 29, 461
AluI AGCT 2 cut(s) 29, 461
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 2 cut(s) 235, 257
Asp700I GAANNNNTTC 1 cut(s) 207
AspLEI GCGC 2 cut(s) 106, 542
AspS9I GGNCC 1 cut(s) 519
AsuHPI GGTGA 2 cut(s) 125, 203
AvaII GGWCC 1 cut(s) 519
BaeI ACNNNNGTAYC 2 cut(s) 108, 141
BanI GGYRCC 1 cut(s) 103
BbvI GCAGC 2 cut(s) 222, 269
BccI CCATC 1 cut(s) 182
BciT130I CCWGG 3 cut(s) 58, 408, 533
BclI TGATCA 1 cut(s) 139
BfaI CTAG 1 cut(s) 437
BfoI RGCGCY 1 cut(s) 107
BisI GCNGC 2 cut(s) 236, 258
BlsI GCNGC 2 cut(s) 237, 259
Bme1390I CCNGG 3 cut(s) 58, 408, 533
Bme18I GGWCC 1 cut(s) 519
BmgT120I GGNCC 1 cut(s) 519
BmiI GGNNCC 3 cut(s) 105, 399, 521
BmrFI CCNGG 3 cut(s) 58, 408, 533
BmsI GCATC 4 cut(s) 10, 210, 386, 498
Bpu10I CCTNAGC 1 cut(s) 447
BpuEI CTTGAG 2 cut(s) 9, 105
BsaHI GRCGYC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 57
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 387, 407
Bse1I ACTGG 2 cut(s) 100, 115
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 3 cut(s) 58, 408, 533
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 2 cut(s) 225, 377
BseLI CCNNNNNNNGG 3 cut(s) 154, 387, 407
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 2 cut(s) 461, 506
BseNI ACTGG 2 cut(s) 100, 115
BseXI GCAGC 2 cut(s) 222, 269
BseYI CCCAGC 1 cut(s) 419
BsgI GTGCAG 2 cut(s) 225, 276
BshFI GGCC 1 cut(s) 17
BshNI GGYRCC 1 cut(s) 103
BslI CCNNNNNNNGG 3 cut(s) 154, 387, 407
BsmI GAATGC 2 cut(s) 207, 209
BsnI GGCC 1 cut(s) 17
Bsp143I GATC 1 cut(s) 139
BspACI CCGC 2 cut(s) 382, 491
BspANI GGCC 1 cut(s) 17
BspCNI CTCAG 2 cut(s) 460, 507
BspLI GGNNCC 3 cut(s) 105, 399, 521
BspT107I GGYRCC 1 cut(s) 103
BsrBI CCGCTC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 2 cut(s) 100, 115
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 1 cut(s) 139
BssNI GRCGYC 1 cut(s) 104
Bst2UI CCWGG 3 cut(s) 58, 408, 533
Bst4CI ACNGT 2 cut(s) 216, 329
BstACI GRCGYC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 3 cut(s) 338, 447, 515
BstF5I GGATG 2 cut(s) 225, 377
BstH2I RGCGCY 1 cut(s) 107
BstHHI GCGC 2 cut(s) 106, 542
BstKTI GATC 1 cut(s) 142
BstMBI GATC 1 cut(s) 139
BstNI CCWGG 3 cut(s) 58, 408, 533
BstNSI RCATGY 1 cut(s) 255
BstSCI CCNGG 3 cut(s) 56, 406, 531
BstV1I GCAGC 2 cut(s) 222, 269
BsuRI GGCC 1 cut(s) 17
BtsCI GGATG 2 cut(s) 225, 377
BtsIMutI CAGTG 2 cut(s) 221, 366
Cac8I GCNNGC 1 cut(s) 240
CfoI GCGC 2 cut(s) 106, 542
Cfr13I GGNCC 1 cut(s) 519
CsiI ACCWGGT 1 cut(s) 406
Csp6I GTAC 2 cut(s) 364, 504
CviAII CATG 4 cut(s) 49, 252, 355, 543
CviJI RGCY 5 cut(s) 17, 29, 290, 353, 461
CviKI_1 RGCY 5 cut(s) 17, 29, 290, 353, 461
CviQI GTAC 2 cut(s) 364, 504
DdeI CTNAG 3 cut(s) 338, 447, 515
DinI GGCGCC 1 cut(s) 105
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 519
EcoRII CCWGG 3 cut(s) 56, 406, 531
EcoT22I ATGCAT 1 cut(s) 209
EgeI GGCGCC 1 cut(s) 105
EheI GGCGCC 1 cut(s) 105
FaeI CATG 4 cut(s) 52, 255, 358, 546
FatI CATG 4 cut(s) 48, 251, 354, 542
FauI CCCGC 1 cut(s) 389
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 236, 258
FokI GGATG 2 cut(s) 232, 364
Fsp4HI GCNGC 2 cut(s) 236, 258
FspBI CTAG 1 cut(s) 437
GlaI GCGC 2 cut(s) 105, 541
GluI GCNGC 2 cut(s) 236, 258
GsaI CCCAGC 1 cut(s) 423
HaeII RGCGCY 1 cut(s) 107
HaeIII GGCC 1 cut(s) 17
HhaI GCGC 2 cut(s) 106, 542
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 4 cut(s) 52, 255, 358, 546
Hin6I GCGC 2 cut(s) 104, 540
HinP1I GCGC 2 cut(s) 104, 540
HinfI GANTC 2 cut(s) 82, 169
HphI GGTGA 2 cut(s) 125, 203
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 4 cut(s) 13, 139, 298, 333
Hpy188III TCNNGA 1 cut(s) 449
Hpy8I GTNNAC 1 cut(s) 364
HpyCH4III ACNGT 2 cut(s) 216, 329
HpyCH4V TGCA 5 cut(s) 127, 207, 242, 257, 497
HpyF3I CTNAG 3 cut(s) 338, 447, 515
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 4 cut(s) 52, 255, 358, 546
HspAI GCGC 2 cut(s) 104, 540
KasI GGCGCC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 1 cut(s) 139
LmnI GCTCC 1 cut(s) 488
Lsp1109I GCAGC 2 cut(s) 222, 269
LweI GCATC 4 cut(s) 10, 210, 386, 498
MabI ACCWGGT 1 cut(s) 406
MaeI CTAG 1 cut(s) 437
MaeIII GTNAC 2 cut(s) 191, 439
MalI GATC 1 cut(s) 141
MbiI CCGCTC 1 cut(s) 491
MboI GATC 1 cut(s) 139
MluCI AATT 2 cut(s) 71, 128
Mly113I GGCGCC 1 cut(s) 104
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 2 cut(s) 289, 466
MnlI CCTC 4 cut(s) 7, 60, 411, 510
Mph1103I ATGCAT 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 359
MspR9I CCNGG 3 cut(s) 58, 408, 533
Mva1269I GAATGC 2 cut(s) 207, 209
MvaI CCWGG 3 cut(s) 58, 408, 533
NarI GGCGCC 1 cut(s) 104
NdeII GATC 1 cut(s) 139
NlaIII CATG 4 cut(s) 52, 255, 358, 546
NlaIV GGNNCC 3 cut(s) 105, 399, 521
NmuCI GTSAC 2 cut(s) 191, 439
NsiI ATGCAT 1 cut(s) 209
NspI RCATGY 1 cut(s) 255
PciI ACATGT 1 cut(s) 251
PctI GAATGC 2 cut(s) 207, 209
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 1 cut(s) 82
PfoI TCCNGGA 1 cut(s) 531
PkrI GCNGC 2 cut(s) 237, 259
PleI GAGTC 1 cut(s) 163
PluTI GGCGCC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 163
PpuMI RGGWCCY 1 cut(s) 519
PscI ACATGT 1 cut(s) 251
Psp5II RGGWCCY 1 cut(s) 519
Psp6I CCWGG 3 cut(s) 56, 406, 531
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 3 cut(s) 56, 406, 531
PspN4I GGNNCC 3 cut(s) 105, 399, 521
PspPI GGNCC 1 cut(s) 519
PspPPI RGGWCCY 1 cut(s) 519
RsaI GTAC 2 cut(s) 365, 505
RsaNI GTAC 2 cut(s) 364, 504
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 2 cut(s) 236, 258
Sau3AI GATC 1 cut(s) 139
Sau96I GGNCC 1 cut(s) 519
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 3 cut(s) 58, 408, 533
SetI ASST 6 cut(s) 31, 59, 392, 399, 409, 463
SexAI ACCWGGT 1 cut(s) 406
SfaNI GCATC 4 cut(s) 10, 210, 386, 498
SfoI GGCGCC 1 cut(s) 105
SinI GGWCC 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 2 cut(s) 24, 120
SmoI CTYRAG 2 cut(s) 24, 120
Sse9I AATT 2 cut(s) 71, 128
SsiI CCGC 2 cut(s) 382, 491
SspDI GGCGCC 1 cut(s) 103
SspMI CTAG 1 cut(s) 437
StyD4I CCNGG 3 cut(s) 56, 406, 531
TaaI ACNGT 2 cut(s) 216, 329
TaqI TCGA 1 cut(s) 372
TasI AATT 2 cut(s) 71, 128
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 2 cut(s) 221, 366
TseFI GTSAC 2 cut(s) 191, 439
TseI GCWGC 2 cut(s) 235, 257
Tsp45I GTSAC 2 cut(s) 191, 439
TspRI CASTG 2 cut(s) 221, 366
VpaK11BI GGWCC 1 cut(s) 519
XceI RCATGY 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 1 cut(s) 437
Zsp2I ATGCAT 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.