RchiOBHm_Chr6g0291881

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0018627
Physical Location & Seq
Reverse (-)
54943946 .. 54948760
4815 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26190

Sequence Viewer

Length: 1044 bp
ATGGAGGCCATACTGAATGAGACTGAAGACGAAGATGAGGAGAAGTTACTAGAGCGATTCTTATCAAAGGAGGAGGATGATGATGGTCCACAGCGTAATCTCGCAGACCTCATACTTGCAACCATTAAGAACAAACAGTATGATGTCACCTATGCTAGGTCTTTGGTTAAAATGGATACAGATTGCATAGAAGTCTACAAGGGGGTTGCAAAGTTTATGAAAAGCTACAGTGCCGGCAAGATTGCCAAACCATTCAAATTTATCCCTGCACTGCAACAATGGGAGGATGCCCTCTATTTCACTGAGACTGATAATTGGTCACCAACTGCTATGTATGAAGCTACAAGAATTCTTGCTTCTAATTTGACTCCACAAAAAGCAGAACGTTTCTACAAGCATGTCTCGCTCCCACGGGTGAGACAAGAAATTCGGAATAAGAAGAAGAAGAAGCCCTTGCATTTTTCCCTGTATCAATCTCTAAGGAAGTCCGTCCACAAGCGTGCTGCCTTTTTTAAAGTAATACTGTTGCCTTTGTGTGAGTCAAGGACATGCACTCTAAAAGAAGCCTTCGTTGTTGGGAGCATCGATCATACAAAAGGTTCGAATTTCTGCAAGTCATGCAGGCTTCTTTTGGAGAAACAGTATGCTTTGCCAAATCCTGTAATCGAGGCTCTTGTTGCACATTTTATGAGATCTGTGGAGGAACCAAGAGTAATGCCTGTGATATGGCACCAGTCTCTTCTTGCATTTGTGCAAAGGAAGCAATTTTCTTCCAATTTTGAGTCATATTTAAGAGTTCATGATTTGTGGTTTTTTTTTCTTTCCAAATTACAGTTGGTGTGCATAATCCCTTGCAGGTACAAAAATGATATAACAAAGGACAACAAAGATAAGCTTAGGAATCTTCTTGAAGCACAAAACCACCGTGAGATTACTCCTGAAATAAGAAGGGAGCTATTTCATAGCTGCAATCATGGTGAGAAGGAGCATGATCATACGTCAAACATGTTTGATATTCCAGAGGTGCCTCTGGAGGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

347

Amino Acids

40.75

Weight (kDa)

8.29

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 30 - 195 1.3e-53 Bystin
Bystin PF05291 208 - 254 3.9e-15 Bystin
Bystin PF05291 285 - 328 5e-10 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018627)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0291881
rosa_multiflora Rmu_sc0002484.1_g000003
rosa_roxburghii Rroxscaffold_7G00175660
rosa_rugosa Rorug06G0221700 Rorug06G0221700
rosa_samantha Rh6AG335100 Rh6BG342500 Rh6CG348600 Rh6DG335300
rosa_wichuraiana Rw6G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 846
AccB1I GGYRCC 2 cut(s) 729, 1024
AccI GTMKAC 1 cut(s) 195
AclI AACGTT 1 cut(s) 385
AcsI RAATTY 4 cut(s) 257, 348, 426, 604
AcuI CTGAAG 1 cut(s) 45
AfaI GTAC 1 cut(s) 860
AfiI CCNNNNNNNGG 1 cut(s) 156
AflIII ACRYGT 1 cut(s) 1005
AgsI TTSAA 2 cut(s) 256, 911
AleI CACNNNNGTG 1 cut(s) 498
AluBI AGCT 5 cut(s) 225, 341, 895, 955, 966
AluI AGCT 5 cut(s) 225, 341, 895, 955, 966
Alw26I GTCTC 5 cut(s) 14, 299, 406, 412, 741
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 503, 966
ApoI RAATTY 4 cut(s) 257, 348, 426, 604
ArsI GACNNNNNNTTYG 2 cut(s) 411, 443
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 4 cut(s) 139, 312, 427, 989
AsuII TTCGAA 1 cut(s) 602
AvaII GGWCC 1 cut(s) 86
BanI GGYRCC 2 cut(s) 729, 1024
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 490, 953
BccI CCATC 1 cut(s) 77
BciVI GTATCC 1 cut(s) 169
BclI TGATCA 1 cut(s) 991
BcoDI GTCTC 5 cut(s) 14, 299, 406, 412, 741
BfaI CTAG 2 cut(s) 50, 156
BfmI CTRYAG 1 cut(s) 226
BfuAI ACCTGC 1 cut(s) 846
BfuI GTATCC 1 cut(s) 169
BglII AGATCT 1 cut(s) 692
BisI GCNGC 2 cut(s) 504, 967
BlsI GCNGC 2 cut(s) 505, 968
Bme18I GGWCC 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 3 cut(s) 705, 731, 1026
BmsI GCATC 2 cut(s) 277, 591
BpiI GAAGAC 1 cut(s) 33
Bpu10I CCTNAGC 1 cut(s) 896
Bpu14I TTCGAA 1 cut(s) 602
Bsa29I ATCGAT 1 cut(s) 585
BsaBI GATNNNNATC 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 1 cut(s) 733
Bse8I GATNNNNATC 1 cut(s) 61
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 1 cut(s) 410
BseGI GGATG 3 cut(s) 82, 292, 1042
BseJI GATNNNNATC 1 cut(s) 61
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 733
BseRI GAGGAG 2 cut(s) 53, 86
BseXI GCAGC 2 cut(s) 490, 953
BsgI GTGCAG 1 cut(s) 252
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 2 cut(s) 729, 1024
BshVI ATCGAT 1 cut(s) 585
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 5 cut(s) 14, 299, 406, 412, 741
BsnI GGCC 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 602
Bsp143I GATC 3 cut(s) 586, 692, 991
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 295
BspDI ATCGAT 1 cut(s) 585
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 3 cut(s) 705, 731, 1026
BspMI ACCTGC 1 cut(s) 846
BspT104I TTCGAA 1 cut(s) 602
BspT107I GGYRCC 2 cut(s) 729, 1024
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 1 cut(s) 733
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 1 cut(s) 410
BssMI GATC 3 cut(s) 586, 692, 991
Bst4CI ACNGT 6 cut(s) 138, 230, 525, 642, 834, 926
Bst6I CTCTTC 1 cut(s) 744
BstAPI GCANNNNNTGC 1 cut(s) 618
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 3 cut(s) 235, 501, 623
BstDEI CTNAG 3 cut(s) 303, 479, 896
BstDSI CCRYGG 1 cut(s) 410
BstEII GGTNACC 1 cut(s) 318
BstENI CCTNNNNNAGG 1 cut(s) 154
BstF5I GGATG 3 cut(s) 82, 292, 1042
BstKTI GATC 3 cut(s) 589, 695, 994
BstMAI GTCTC 5 cut(s) 14, 299, 406, 412, 741
BstMBI GATC 3 cut(s) 586, 692, 991
BstMWI GCNNNNNNNGC 4 cut(s) 403, 618, 677, 760
BstNSI RCATGY 3 cut(s) 401, 552, 1009
BstPI GGTNACC 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 226
BstV1I GCAGC 2 cut(s) 490, 953
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 692
BstYI RGATCY 1 cut(s) 692
Bsu15I ATCGAT 1 cut(s) 585
BsuI GTATCC 1 cut(s) 169
BsuRI GGCC 1 cut(s) 8
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 1 cut(s) 410
BtsCI GGATG 3 cut(s) 82, 292, 1042
BtsI GCAGTG 1 cut(s) 269
BtsIMutI CAGTG 3 cut(s) 235, 269, 300
BveI ACCTGC 1 cut(s) 846
Cac8I GCNNGC 3 cut(s) 235, 501, 623
CciI TCATGA 1 cut(s) 799
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 86
ClaI ATCGAT 1 cut(s) 585
Csp6I GTAC 1 cut(s) 859
CviAII CATG 7 cut(s) 398, 549, 618, 800, 974, 989, 1006
CviQI GTAC 1 cut(s) 859
DdeI CTNAG 3 cut(s) 303, 479, 896
DpnI GATC 3 cut(s) 588, 694, 993
DpnII GATC 3 cut(s) 586, 692, 991
DraI TTTAAA 1 cut(s) 514
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 45
Eco91I GGTNACC 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 154
EcoO65I GGTNACC 1 cut(s) 318
EcoRI GAATTC 1 cut(s) 348
FaeI CATG 7 cut(s) 401, 552, 621, 803, 977, 992, 1009
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 879, 911
FatI CATG 7 cut(s) 397, 548, 617, 799, 973, 988, 1005
FbaI TGATCA 1 cut(s) 991
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 2 cut(s) 504, 967
FokI GGATG 2 cut(s) 89, 299
Fsp4HI GCNGC 2 cut(s) 504, 967
FspBI CTAG 2 cut(s) 50, 156
GluI GCNGC 2 cut(s) 504, 967
HaeIII GGCC 1 cut(s) 8
HapII CCGG 1 cut(s) 234
Hin1II CATG 7 cut(s) 401, 552, 621, 803, 977, 992, 1009
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 5 cut(s) 57, 367, 539, 782, 901
HpaII CCGG 1 cut(s) 234
HphI GGTGA 4 cut(s) 139, 312, 427, 989
Hpy166II GTNNAC 3 cut(s) 89, 196, 493
Hpy188I TCNGA 1 cut(s) 432
Hpy188III TCNNGA 5 cut(s) 800, 908, 938, 1019, 1031
Hpy8I GTNNAC 3 cut(s) 89, 196, 493
HpyAV CCTTC 3 cut(s) 577, 942, 976
HpyCH4III ACNGT 6 cut(s) 138, 230, 525, 642, 834, 926
HpyCH4IV ACGT 2 cut(s) 385, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 403, 618, 677, 760
HpyF3I CTNAG 3 cut(s) 303, 479, 896
HpySE526I ACGT 2 cut(s) 385, 998
Hsp92II CATG 7 cut(s) 401, 552, 621, 803, 977, 992, 1009
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 991
Kzo9I GATC 3 cut(s) 586, 692, 991
LmnI GCTCC 4 cut(s) 411, 579, 952, 985
Lsp1109I GCAGC 2 cut(s) 490, 953
LweI GCATC 2 cut(s) 277, 591
MaeI CTAG 2 cut(s) 50, 156
MaeII ACGT 2 cut(s) 385, 998
MaeIII GTNAC 3 cut(s) 45, 145, 318
MalI GATC 3 cut(s) 588, 694, 993
MboI GATC 3 cut(s) 586, 692, 991
MboII GAAGA 8 cut(s) 38, 44, 451, 454, 457, 731, 762, 896
MflI RGATCY 1 cut(s) 692
MluCI AATT 9 cut(s) 257, 313, 348, 361, 426, 604, 764, 775, 827
MlyI GAGTC 3 cut(s) 361, 548, 791
MroNI GCCGGC 1 cut(s) 233
MseI TTAA 4 cut(s) 126, 168, 513, 791
MslI CAYNNNNRTG 1 cut(s) 498
MspI CCGG 1 cut(s) 234
MwoI GCNNNNNNNGC 4 cut(s) 403, 618, 677, 760
NaeI GCCGGC 1 cut(s) 235
NdeII GATC 3 cut(s) 586, 692, 991
NgoMIV GCCGGC 1 cut(s) 233
NlaIII CATG 7 cut(s) 401, 552, 621, 803, 977, 992, 1009
NlaIV GGNNCC 3 cut(s) 705, 731, 1026
NmuCI GTSAC 2 cut(s) 145, 318
NspI RCATGY 3 cut(s) 401, 552, 1009
NspV TTCGAA 1 cut(s) 602
OliI CACNNNNGTG 1 cut(s) 498
PagI TCATGA 1 cut(s) 799
PciI ACATGT 1 cut(s) 1005
PdiI GCCGGC 1 cut(s) 235
PfeI GAWTC 2 cut(s) 57, 901
PkrI GCNGC 2 cut(s) 505, 968
PleI GAGTC 3 cut(s) 361, 547, 790
PpsI GAGTC 3 cut(s) 361, 547, 790
PscI ACATGT 1 cut(s) 1005
Psp1406I AACGTT 1 cut(s) 385
PspEI GGTNACC 1 cut(s) 318
PspN4I GGNNCC 3 cut(s) 705, 731, 1026
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 1 cut(s) 692
RsaI GTAC 1 cut(s) 860
RsaNI GTAC 1 cut(s) 859
RseI CAYNNNNRTG 1 cut(s) 498
SaqAI TTAA 4 cut(s) 126, 168, 513, 791
SatI GCNGC 2 cut(s) 504, 967
Sau3AI GATC 3 cut(s) 586, 692, 991
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 3 cut(s) 361, 548, 791
SfaNI GCATC 2 cut(s) 277, 591
SfcI CTRYAG 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 498
Sse9I AATT 9 cut(s) 257, 313, 348, 361, 426, 604, 764, 775, 827
SspMI CTAG 2 cut(s) 50, 156
TaaI ACNGT 6 cut(s) 138, 230, 525, 642, 834, 926
TaiI ACGT 2 cut(s) 388, 1001
TaqI TCGA 3 cut(s) 585, 602, 666
TasI AATT 9 cut(s) 257, 313, 348, 361, 426, 604, 764, 775, 827
TfiI GAWTC 2 cut(s) 57, 901
Tru1I TTAA 4 cut(s) 126, 168, 513, 791
Tru9I TTAA 4 cut(s) 126, 168, 513, 791
TscAI CASTG 3 cut(s) 235, 276, 307
TseFI GTSAC 2 cut(s) 145, 318
TseI GCWGC 2 cut(s) 503, 966
Tsp45I GTSAC 2 cut(s) 145, 318
TspDTI ATGAA 4 cut(s) 233, 351, 788, 950
TspGWI ACGGA 1 cut(s) 478
TspRI CASTG 3 cut(s) 235, 276, 307
VpaK11BI GGWCC 1 cut(s) 86
XagI CCTNNNNNAGG 1 cut(s) 154
XapI RAATTY 4 cut(s) 257, 348, 426, 604
XceI RCATGY 3 cut(s) 401, 552, 1009
XcmI CCANNNNNNNNNTGG 1 cut(s) 832
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 2 cut(s) 50, 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.