Rh6AG335100

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018627
Physical Location & Seq
Reverse (-)
53574915 .. 53578523
3609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG335100.1

Sequence Viewer

Length: 1086 bp
ATGACGAAGAAGAGGGGGAAAGAGAGGCATCGGAATCCAAAGCCGTTCCTCGCCGAAGACGATGTCTCTGTGGCTTCTACACAGGTAACCAAACAGCCCAAAATGGCGCTCCAGCGCCAAGAGAATCTAGTTTTATCAGCAGGCATGAGCTCGAAAATCCTCAGAGAAGCTCAGATTCAGGAGAAGGAGATCGAGGAAGAGGAGAACACAAACGCCTTCACTGAAGAGCCGCACAACGATGAAGACTTTGATACGTTCCTTGAAACACATAGAGATGATGAATCAAACAAAGTGATGGAGGCCATACTGAATGAGACTGAAGACGAAGATGAGGAGAAGTTACTAGAGCGATTCTTATCAAAGGAGGAGGGTGATGATGGTCCACAGCGTAATCTCGCAGACCTCATACTTGCAACCATTAAGAACAAACAGTATGATGTCACCTCAGATGCTAGGTCTTTGGTTAAAATGGATACAGATTGCATAGAAGTCTACAAGGGGGTTGCAAAGTTTATGAAAAGCTACAGTGCCGGCAAGATTGCCAAACCATTCAAATTTATCCCTGCAATGCAACAATGGGAGGATGCCCTCTTTTCACTGAGCCTGATAATTGGTCACCAACAGCTATTCAAGGACATGCACTCTAAAAGAAGCCTCCGTTGTTGGGAGCATCATACAAAAGTTCGAATTTCTGCAAGTCATGCAGGTGCTGCGATAATGATGCAATATAGCAGCGCAAGAAGTTATTTTATAAAGCTTCTTTTGGAGAAACAGTATGCTTTGCCAAATCCTGTAATCGAGGCTCTTGTTGCACATTTTATGAGATCTGTGGAGGAACCAAGAGTAATGCCTGTGATATGGCACCAGTCTCTTCTTGCATTTGTGCAAAGGTACAAAAATGATATAACAAAGGACAACAAAGATAAGCTTAGGAATCTTCTTGAAGCACAAAACCACCATGAGATTACTCCTGAAATAAGAAGGGAGCTATTTCATAGCTGCAATGATGGTGAGAAGGAGCATGATCGTGCGTCAAGTGATATCCTTCTTTTATTCTTGTTTTCTGTTATAATTAAATATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

361

Amino Acids

41.94

Weight (kDa)

5.83

Isoelectric Point (pI)

54.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 128 - 197 1.9e-12 Bystin
Bystin PF05291 226 - 338 3.1e-39 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018627)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0291881
rosa_multiflora Rmu_sc0002484.1_g000003
rosa_roxburghii Rroxscaffold_7G00175660
rosa_rugosa Rorug06G0221700 Rorug06G0221700
rosa_samantha Rh6AG335100 Rh6BG342500 Rh6CG348600 Rh6DG335300
rosa_wichuraiana Rw6G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 752, 1070
AarI CACCTGC 1 cut(s) 695
Acc36I ACCTGC 1 cut(s) 695
AccB1I GGYRCC 1 cut(s) 861
AccI GTMKAC 1 cut(s) 492
AciI CCGC 1 cut(s) 230
AcsI RAATTY 2 cut(s) 554, 687
AcuI CTGAAG 2 cut(s) 243, 339
AfaI GTAC 1 cut(s) 893
AfiI CCNNNNNNNGG 1 cut(s) 664
AgsI TTSAA 4 cut(s) 263, 553, 631, 944
AluBI AGCT 8 cut(s) 150, 170, 522, 625, 757, 928, 988, 999
AluI AGCT 8 cut(s) 150, 170, 522, 625, 757, 928, 988, 999
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 3 cut(s) 70, 308, 873
AlwNI CAGNNNCTG 1 cut(s) 710
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 3 cut(s) 710, 732, 999
ApoI RAATTY 2 cut(s) 554, 687
ArsI GACNNNNNNTTYG 2 cut(s) 48, 80
AspLEI GCGC 3 cut(s) 109, 117, 737
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 4 cut(s) 383, 433, 608, 1022
AsuII TTCGAA 1 cut(s) 685
AvaII GGWCC 1 cut(s) 380
BanI GGYRCC 1 cut(s) 861
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 3 cut(s) 63, 249, 327
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 3 cut(s) 697, 744, 986
BccI CCATC 3 cut(s) 289, 371, 1001
BceAI ACGGC 1 cut(s) 28
BcgI CGANNNNNNTGC 2 cut(s) 703, 737
BciVI GTATCC 1 cut(s) 466
BcoDI GTCTC 3 cut(s) 70, 308, 873
BfaI CTAG 3 cut(s) 128, 344, 453
BfmI CTRYAG 1 cut(s) 523
BfoI RGCGCY 2 cut(s) 110, 118
BfuAI ACCTGC 1 cut(s) 695
BfuI GTATCC 1 cut(s) 466
BglII AGATCT 1 cut(s) 824
BisI GCNGC 4 cut(s) 230, 711, 733, 1000
BlsI GCNGC 4 cut(s) 231, 712, 734, 1001
Bme18I GGWCC 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 2 cut(s) 837, 863
BmsI GCATC 5 cut(s) 37, 439, 574, 679, 711
BpiI GAAGAC 3 cut(s) 63, 249, 327
BpmI CTGGAG 1 cut(s) 95
Bpu10I CCTNAGC 1 cut(s) 929
Bpu14I TTCGAA 1 cut(s) 685
BsaBI GATNNNNATC 1 cut(s) 355
BsaXI ACNNNNNCTCC 2 cut(s) 758, 788
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 865
Bse3DI GCAATG 2 cut(s) 573, 1009
Bse8I GATNNNNATC 1 cut(s) 355
BseGI GGATG 1 cut(s) 589
BseJI GATNNNNATC 1 cut(s) 355
BseLI CCNNNNNNNGG 1 cut(s) 664
BseMI GCAATG 2 cut(s) 573, 1009
BseMII CTCAG 4 cut(s) 175, 185, 459, 590
BseNI ACTGG 1 cut(s) 865
BseRI GAGGAG 3 cut(s) 215, 347, 380
BseXI GCAGC 3 cut(s) 697, 744, 986
BshFI GGCC 1 cut(s) 302
BshNI GGYRCC 1 cut(s) 861
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 531
BslI CCNNNNNNNGG 1 cut(s) 664
BsmAI GTCTC 3 cut(s) 70, 308, 873
BsnI GGCC 1 cut(s) 302
Bsp119I TTCGAA 1 cut(s) 685
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 3 cut(s) 189, 824, 1024
BspACI CCGC 1 cut(s) 230
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 4 cut(s) 174, 184, 458, 591
BspLI GGNNCC 2 cut(s) 837, 863
BspMI ACCTGC 1 cut(s) 695
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 685
BspT107I GGYRCC 1 cut(s) 861
BsrDI GCAATG 2 cut(s) 573, 1009
BsrFI RCCGGY 1 cut(s) 530
BsrI ACTGG 1 cut(s) 865
BssAI RCCGGY 1 cut(s) 530
BssMI GATC 3 cut(s) 189, 824, 1024
Bst4CI ACNGT 3 cut(s) 432, 527, 774
Bst6I CTCTTC 4 cut(s) 5, 192, 219, 876
BstAPI GCANNNNNTGC 2 cut(s) 701, 710
BstBI TTCGAA 1 cut(s) 685
BstC8I GCNNGC 2 cut(s) 142, 532
BstDEI CTNAG 5 cut(s) 161, 171, 445, 599, 929
BstEII GGTNACC 2 cut(s) 85, 614
BstF5I GGATG 1 cut(s) 589
BstH2I RGCGCY 2 cut(s) 110, 118
BstHHI GCGC 3 cut(s) 109, 117, 737
BstKTI GATC 3 cut(s) 192, 827, 1027
BstMAI GTCTC 3 cut(s) 70, 308, 873
BstMBI GATC 3 cut(s) 189, 824, 1024
BstMWI GCNNNNNNNGC 3 cut(s) 701, 710, 809
BstNSI RCATGY 1 cut(s) 640
BstPI GGTNACC 2 cut(s) 85, 614
BstSFI CTRYAG 1 cut(s) 523
BstV1I GCAGC 3 cut(s) 697, 744, 986
BstV2I GAAGAC 3 cut(s) 63, 249, 327
BstX2I RGATCY 1 cut(s) 824
BstYI RGATCY 1 cut(s) 824
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 1 cut(s) 302
BtsCI GGATG 1 cut(s) 589
BtsIMutI CAGTG 3 cut(s) 219, 532, 596
BveI ACCTGC 1 cut(s) 695
Cac8I GCNNGC 2 cut(s) 142, 532
CaiI CAGNNNCTG 1 cut(s) 710
CfoI GCGC 3 cut(s) 109, 117, 737
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 380
CseI GACGC 1 cut(s) 1020
Csp6I GTAC 1 cut(s) 892
CviAII CATG 5 cut(s) 145, 637, 701, 959, 1022
CviQI GTAC 1 cut(s) 892
DdeI CTNAG 5 cut(s) 161, 171, 445, 599, 929
DpnI GATC 3 cut(s) 191, 826, 1026
DpnII GATC 3 cut(s) 189, 824, 1024
Eam1104I CTCTTC 4 cut(s) 5, 192, 219, 876
EarI CTCTTC 4 cut(s) 5, 192, 219, 876
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco32I GATATC 1 cut(s) 1042
Eco47I GGWCC 1 cut(s) 380
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 243, 339
Eco91I GGTNACC 2 cut(s) 85, 614
EcoICRI GAGCTC 1 cut(s) 150
EcoO65I GGTNACC 2 cut(s) 85, 614
EcoRV GATATC 1 cut(s) 1042
EcoT38I GRGCYC 1 cut(s) 152
FaeI CATG 5 cut(s) 148, 640, 704, 962, 1025
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FatI CATG 5 cut(s) 144, 636, 700, 958, 1021
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 4 cut(s) 230, 711, 733, 1000
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 4 cut(s) 230, 711, 733, 1000
FspBI CTAG 3 cut(s) 128, 344, 453
GlaI GCGC 3 cut(s) 108, 116, 736
GluI GCNGC 4 cut(s) 230, 711, 733, 1000
GsuI CTGGAG 1 cut(s) 95
HaeII RGCGCY 2 cut(s) 110, 118
HaeIII GGCC 1 cut(s) 302
HapII CCGG 1 cut(s) 531
HgaI GACGC 1 cut(s) 1020
HhaI GCGC 3 cut(s) 109, 117, 737
Hin1II CATG 5 cut(s) 148, 640, 704, 962, 1025
Hin6I GCGC 3 cut(s) 107, 115, 735
HinP1I GCGC 3 cut(s) 107, 115, 735
HindIII AAGCTT 2 cut(s) 755, 926
HinfI GANTC 6 cut(s) 34, 124, 175, 281, 351, 934
HpaII CCGG 1 cut(s) 531
HphI GGTGA 4 cut(s) 383, 433, 608, 1022
Hpy166II GTNNAC 2 cut(s) 383, 493
Hpy188I TCNGA 4 cut(s) 33, 164, 174, 448
Hpy188III TCNNGA 3 cut(s) 179, 941, 971
Hpy8I GTNNAC 2 cut(s) 383, 493
HpyAV CCTTC 5 cut(s) 178, 226, 975, 1009, 1055
HpyCH4III ACNGT 3 cut(s) 432, 527, 774
HpyCH4IV ACGT 1 cut(s) 254
HpyF10VI GCNNNNNNNGC 3 cut(s) 701, 710, 809
HpyF3I CTNAG 5 cut(s) 161, 171, 445, 599, 929
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 5 cut(s) 148, 640, 704, 962, 1025
HspAI GCGC 3 cut(s) 107, 115, 735
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Kzo9I GATC 3 cut(s) 189, 824, 1024
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 4 cut(s) 114, 667, 985, 1018
Lsp1109I GCAGC 3 cut(s) 697, 744, 986
LweI GCATC 5 cut(s) 37, 439, 574, 679, 711
MaeI CTAG 3 cut(s) 128, 344, 453
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 4 cut(s) 85, 339, 439, 614
MalI GATC 3 cut(s) 191, 826, 1026
MboI GATC 3 cut(s) 189, 824, 1024
MflI RGATCY 1 cut(s) 824
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 4 cut(s) 554, 609, 687, 1071
MroNI GCCGGC 1 cut(s) 530
MseI TTAA 3 cut(s) 420, 465, 1074
MslI CAYNNNNRTG 4 cut(s) 237, 273, 705, 1026
MspI CCGG 1 cut(s) 531
MwoI GCNNNNNNNGC 3 cut(s) 701, 710, 809
NaeI GCCGGC 1 cut(s) 532
NdeII GATC 3 cut(s) 189, 824, 1024
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 5 cut(s) 148, 640, 704, 962, 1025
NlaIV GGNNCC 2 cut(s) 837, 863
NmuCI GTSAC 2 cut(s) 439, 614
NspI RCATGY 1 cut(s) 640
NspV TTCGAA 1 cut(s) 685
PaqCI CACCTGC 1 cut(s) 695
PciSI GCTCTTC 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PdiI GCCGGC 1 cut(s) 532
PfeI GAWTC 6 cut(s) 34, 124, 175, 281, 351, 934
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 4 cut(s) 231, 712, 734, 1001
PsiI TTATAA 2 cut(s) 752, 1070
Psp124BI GAGCTC 1 cut(s) 152
PspEI GGTNACC 2 cut(s) 85, 614
PspN4I GGNNCC 2 cut(s) 837, 863
PspPI GGNCC 1 cut(s) 380
PstNI CAGNNNCTG 1 cut(s) 710
PsuI RGATCY 1 cut(s) 824
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 1 cut(s) 893
RsaNI GTAC 1 cut(s) 892
RseI CAYNNNNRTG 4 cut(s) 237, 273, 705, 1026
SacI GAGCTC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 3 cut(s) 420, 465, 1074
SatI GCNGC 4 cut(s) 230, 711, 733, 1000
Sau3AI GATC 3 cut(s) 189, 824, 1024
Sau96I GGNCC 1 cut(s) 380
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 5 cut(s) 37, 439, 574, 679, 711
SfcI CTRYAG 1 cut(s) 523
SfuI TTCGAA 1 cut(s) 685
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 4 cut(s) 237, 273, 705, 1026
Sse9I AATT 4 cut(s) 554, 609, 687, 1071
SsiI CCGC 1 cut(s) 230
SspI AATATT 1 cut(s) 1079
SspMI CTAG 3 cut(s) 128, 344, 453
SstI GAGCTC 1 cut(s) 152
TaaI ACNGT 3 cut(s) 432, 527, 774
TaiI ACGT 1 cut(s) 257
TaqI TCGA 4 cut(s) 152, 192, 685, 798
TasI AATT 4 cut(s) 554, 609, 687, 1071
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 6 cut(s) 34, 124, 175, 281, 351, 934
Tru1I TTAA 3 cut(s) 420, 465, 1074
Tru9I TTAA 3 cut(s) 420, 465, 1074
TscAI CASTG 3 cut(s) 226, 532, 603
TseFI GTSAC 2 cut(s) 439, 614
TseI GCWGC 3 cut(s) 710, 732, 999
Tsp45I GTSAC 2 cut(s) 439, 614
TspDTI ATGAA 4 cut(s) 255, 294, 530, 983
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 3 cut(s) 226, 532, 603
Tth111I GACNNNGTC 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 2 cut(s) 554, 687
XceI RCATGY 1 cut(s) 640
XmiI GTMKAC 1 cut(s) 492
XspI CTAG 3 cut(s) 128, 344, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.