Rh6BG342500

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0018627
Physical Location & Seq
Reverse (-)
57452047 .. 57456140
4094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG342500.1

Sequence Viewer

Length: 1278 bp
ATGACGAAGAAGAGGGGGAGAGAGAGGCATCGAAATCCAAAGCCGTTCCTCGCCGAAGACGATGTCTCTGTGGCTTCCACAAAGCTAACCAAACAGCCCAAAATGGTGCGCAAGCGCCAAGAGAATCTAGTTTTATCAGCAGGCATGAGCTCGAAAATCCTCAGGGAAGCTCAGATTCAGGAGAAGGAGATCGAGGAAGAGGAGAACACAAACGCCTTCACTGAAGAGCCGCACAACGATGAAGAGGACGACGATGACTTTGATACGTTCCTTGAAACACATAGAGAGGATGAAGCAAACAAAGTGATGGAGGCCATAATGAATGAGACTGAAGATGAAGATGAGGAGAAGTTACTAGAGCGATTCTTATCAAAGGAGGAGGGTGATGATGGTCCACAGCGTAATCTCGCAGACCTCGTACTTGCAACCATTAAGAACAAACAGTATGATGTCACCTCAGATGCTAGGTCTTTCGTTAAAATGGATACAGATTTCATGGAAGTCTACAAGGGGGTTGCAAAGTTTATGAAAAGCTACAGTGCCGGCAAGATTGCCAAACCATTCAAATTTATCCCGGCAATGCAACAATGGGAGGATGCCCTCTATTTCACTGAGCCTGATAATTGGTCACCAACTGCTATGTATGAAGCTACAAGAATTCTTGCTTCTAATTTGACTCCGCAAAAAGCAGAACGTTTCTACAAGTATGTCTTGCTCCCACGGGTGAGACGAGACATTCGGAATAAGAAGAAGAAGAAGCCCTTGCATTTTTCCCTGTATCAATCTCTAAGGAAGTCCGTCCACAAGCGTGCTGCCTTTTTTAAAGGAATACTGTTGCCTTTGTGTGAGTCAAGGACATGCACTCTAAAAGAAGCCTCCGTTGTTGGGAGCATCATACAAAAGGTTCGAATTTCTGCAAGTCATGCAGGTGCTGCAATGATGATGCTAGCAGAGATGGAATATAGCAGCGCAAGAAGTTATTTTATAAAGCTTCTTTTGGAGAAACAGTATGCTTTGCCAAATCCTGTAATCGAGGCTCTTGTTGCACATTTTATGAGATCTGTGGAGGAACCAAGAGTAATGCCTGTGATATGGCACCAGTCTCTTCTTGCATTTGTGGAAAGGTACAAAAATGATATAACAAAGGACAACAAAGATAAGCTTAGGAATCTTCTTGAAGCACAAAACCACCGTGAGGTGGGTCTGGCTAAAGAAATAATGCACAAGTACATGCTTGGGAGGAAATGCAATACTGTTCTTGCATTGGAATGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

425

Amino Acids

49.45

Weight (kDa)

8.15

Isoelectric Point (pI)

52.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 132 - 410 3.1e-104 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018627)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0291881
rosa_multiflora Rmu_sc0002484.1_g000003
rosa_roxburghii Rroxscaffold_7G00175660
rosa_rugosa Rorug06G0221700 Rorug06G0221700
rosa_samantha Rh6AG335100 Rh6BG342500 Rh6CG348600 Rh6DG335300
rosa_wichuraiana Rw6G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 986
AarI CACCTGC 1 cut(s) 917
Acc16I TGCGCA 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 917
AccB1I GGYRCC 1 cut(s) 1095
AccI GTMKAC 1 cut(s) 504
AciI CCGC 2 cut(s) 230, 680
AclI AACGTT 1 cut(s) 694
AcsI RAATTY 3 cut(s) 566, 657, 909
AcuI CTGAAG 2 cut(s) 243, 351
AfaI GTAC 3 cut(s) 420, 1127, 1229
AfiI CCNNNNNNNGG 3 cut(s) 885, 1195, 1198
AgsI TTSAA 3 cut(s) 275, 565, 1178
AleI CACNNNNGTG 1 cut(s) 807
AluBI AGCT 7 cut(s) 85, 150, 170, 534, 650, 991, 1162
AluI AGCT 7 cut(s) 85, 150, 170, 534, 650, 991, 1162
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 5 cut(s) 70, 320, 721, 726, 1107
AlwNI CAGNNNCTG 1 cut(s) 932
AoxI GGCC 1 cut(s) 312
ApeKI GCWGC 3 cut(s) 812, 932, 966
ApoI RAATTY 3 cut(s) 566, 657, 909
ArsI GACNNNNNNTTYG 6 cut(s) 48, 80, 242, 274, 720, 752
AspLEI GCGC 3 cut(s) 111, 117, 971
AspS9I GGNCC 1 cut(s) 392
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 4 cut(s) 395, 445, 621, 736
AsuII TTCGAA 1 cut(s) 907
AsuNHI GCTAGC 1 cut(s) 946
AvaII GGWCC 1 cut(s) 392
AxyI CCTNAGG 1 cut(s) 161
BanI GGYRCC 1 cut(s) 1095
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 3 cut(s) 799, 919, 978
BccI CCATC 3 cut(s) 301, 383, 949
BceAI ACGGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 478
BcnI CCSGG 1 cut(s) 575
BcoDI GTCTC 5 cut(s) 70, 320, 721, 726, 1107
BfaI CTAG 4 cut(s) 128, 356, 465, 947
BfmI CTRYAG 1 cut(s) 535
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 917
BfuI GTATCC 1 cut(s) 478
BglII AGATCT 1 cut(s) 1058
BisI GCNGC 4 cut(s) 230, 813, 933, 967
BlsI GCNGC 4 cut(s) 231, 814, 934, 968
Bme1390I CCNGG 1 cut(s) 575
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 2 cut(s) 1071, 1097
BmrFI CCNGG 1 cut(s) 575
BmsI GCATC 5 cut(s) 37, 451, 586, 900, 933
BmtI GCTAGC 1 cut(s) 950
BpiI GAAGAC 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 1163
Bpu14I TTCGAA 1 cut(s) 907
BpuMI CCSGG 1 cut(s) 575
BsaBI GATNNNNATC 1 cut(s) 367
BsaJI CCNNGG 1 cut(s) 719
BsaXI ACNNNNNCTCC 2 cut(s) 992, 1022
Bsc4I CCNNNNNNNGG 3 cut(s) 885, 1195, 1198
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 1 cut(s) 1099
Bse21I CCTNAGG 1 cut(s) 161
Bse3DI GCAATG 2 cut(s) 585, 942
Bse8I GATNNNNATC 1 cut(s) 367
BseDI CCNNGG 1 cut(s) 719
BseGI GGATG 2 cut(s) 295, 601
BseJI GATNNNNATC 1 cut(s) 367
BseLI CCNNNNNNNGG 3 cut(s) 885, 1195, 1198
BseMI GCAATG 2 cut(s) 585, 942
BseMII CTCAG 4 cut(s) 175, 185, 471, 603
BseNI ACTGG 1 cut(s) 1099
BseRI GAGGAG 3 cut(s) 215, 359, 392
BseXI GCAGC 3 cut(s) 799, 919, 978
BshFI GGCC 1 cut(s) 314
BshNI GGYRCC 1 cut(s) 1095
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 2 cut(s) 543, 575
BslI CCNNNNNNNGG 3 cut(s) 885, 1195, 1198
BsmAI GTCTC 5 cut(s) 70, 320, 721, 726, 1107
BsmBI CGTCTC 1 cut(s) 721
BsnI GGCC 1 cut(s) 314
Bsp119I TTCGAA 1 cut(s) 907
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 2 cut(s) 189, 1058
BspACI CCGC 2 cut(s) 230, 680
BspANI GGCC 1 cut(s) 314
BspCNI CTCAG 4 cut(s) 174, 184, 470, 604
BspLI GGNNCC 2 cut(s) 1071, 1097
BspMI ACCTGC 1 cut(s) 917
BspOI GCTAGC 1 cut(s) 950
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 907
BspT107I GGYRCC 1 cut(s) 1095
BsrDI GCAATG 2 cut(s) 585, 942
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 1 cut(s) 1099
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 1 cut(s) 719
BssMI GATC 2 cut(s) 189, 1058
Bst4CI ACNGT 6 cut(s) 444, 539, 834, 1008, 1193, 1255
Bst6I CTCTTC 5 cut(s) 5, 192, 219, 237, 1110
BstAPI GCANNNNNTGC 2 cut(s) 923, 932
BstBI TTCGAA 1 cut(s) 907
BstC8I GCNNGC 5 cut(s) 113, 142, 544, 810, 948
BstDEI CTNAG 6 cut(s) 161, 171, 457, 612, 788, 1163
BstDSI CCRYGG 1 cut(s) 719
BstEII GGTNACC 1 cut(s) 627
BstF5I GGATG 2 cut(s) 295, 601
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 3 cut(s) 111, 117, 971
BstKTI GATC 2 cut(s) 192, 1061
BstMAI GTCTC 5 cut(s) 70, 320, 721, 726, 1107
BstMBI GATC 2 cut(s) 189, 1058
BstMWI GCNNNNNNNGC 3 cut(s) 923, 932, 1043
BstNSI RCATGY 2 cut(s) 861, 1234
BstPI GGTNACC 1 cut(s) 627
BstSCI CCNGG 1 cut(s) 573
BstSFI CTRYAG 1 cut(s) 535
BstV1I GCAGC 3 cut(s) 799, 919, 978
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 1058
BstYI RGATCY 1 cut(s) 1058
Bsu36I CCTNAGG 1 cut(s) 161
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 1 cut(s) 314
BtgI CCRYGG 1 cut(s) 719
BtsCI GGATG 2 cut(s) 295, 601
BtsIMutI CAGTG 3 cut(s) 219, 544, 609
BveI ACCTGC 1 cut(s) 917
Cac8I GCNNGC 5 cut(s) 113, 142, 544, 810, 948
CaiI CAGNNNCTG 1 cut(s) 932
CfoI GCGC 3 cut(s) 111, 117, 971
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 392
Csp6I GTAC 3 cut(s) 419, 1126, 1228
CviAII CATG 5 cut(s) 145, 496, 858, 923, 1231
CviQI GTAC 3 cut(s) 419, 1126, 1228
DdeI CTNAG 6 cut(s) 161, 171, 457, 612, 788, 1163
DpnI GATC 2 cut(s) 191, 1060
DpnII GATC 2 cut(s) 189, 1058
DraI TTTAAA 1 cut(s) 823
Eam1104I CTCTTC 5 cut(s) 5, 192, 219, 237, 1110
EarI CTCTTC 5 cut(s) 5, 192, 219, 237, 1110
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 243, 351
Eco81I CCTNAGG 1 cut(s) 161
Eco91I GGTNACC 1 cut(s) 627
EcoICRI GAGCTC 1 cut(s) 150
EcoO65I GGTNACC 1 cut(s) 627
EcoRI GAATTC 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 152
Esp3I CGTCTC 1 cut(s) 721
FaeI CATG 5 cut(s) 148, 499, 861, 926, 1234
FalI AAGNNNNNCTT 6 cut(s) 695, 727, 746, 778, 1146, 1178
FatI CATG 5 cut(s) 144, 495, 857, 922, 1230
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 4 cut(s) 230, 813, 933, 967
FokI GGATG 2 cut(s) 302, 608
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 4 cut(s) 230, 813, 933, 967
FspBI CTAG 4 cut(s) 128, 356, 465, 947
FspI TGCGCA 1 cut(s) 110
GlaI GCGC 3 cut(s) 110, 116, 970
GluI GCNGC 4 cut(s) 230, 813, 933, 967
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 314
HapII CCGG 2 cut(s) 543, 575
HhaI GCGC 3 cut(s) 111, 117, 971
Hin1II CATG 5 cut(s) 148, 499, 861, 926, 1234
Hin6I GCGC 3 cut(s) 109, 115, 969
HinP1I GCGC 3 cut(s) 109, 115, 969
HindIII AAGCTT 2 cut(s) 989, 1160
HinfI GANTC 6 cut(s) 124, 175, 363, 676, 848, 1168
HpaII CCGG 2 cut(s) 543, 575
HphI GGTGA 4 cut(s) 395, 445, 621, 736
Hpy166II GTNNAC 3 cut(s) 395, 505, 802
Hpy188I TCNGA 3 cut(s) 174, 460, 741
Hpy188III TCNNGA 2 cut(s) 179, 1175
Hpy8I GTNNAC 3 cut(s) 395, 505, 802
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 2 cut(s) 178, 226
HpyCH4III ACNGT 6 cut(s) 444, 539, 834, 1008, 1193, 1255
HpyCH4IV ACGT 2 cut(s) 266, 694
HpyF10VI GCNNNNNNNGC 3 cut(s) 923, 932, 1043
HpyF3I CTNAG 6 cut(s) 161, 171, 457, 612, 788, 1163
HpySE526I ACGT 2 cut(s) 266, 694
Hsp92II CATG 5 cut(s) 148, 499, 861, 926, 1234
HspAI GCGC 3 cut(s) 109, 115, 969
KroI GCCGGC 1 cut(s) 542
KroNI GCCGGC 1 cut(s) 544
Kzo9I GATC 2 cut(s) 189, 1058
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 2 cut(s) 720, 888
Lsp1109I GCAGC 3 cut(s) 799, 919, 978
LweI GCATC 5 cut(s) 37, 451, 586, 900, 933
MaeI CTAG 4 cut(s) 128, 356, 465, 947
MaeII ACGT 2 cut(s) 266, 694
MaeIII GTNAC 3 cut(s) 351, 451, 627
MalI GATC 2 cut(s) 191, 1060
MboI GATC 2 cut(s) 189, 1058
MflI RGATCY 1 cut(s) 1058
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 5 cut(s) 566, 622, 657, 670, 909
MlyI GAGTC 2 cut(s) 670, 857
MroNI GCCGGC 1 cut(s) 542
MseI TTAA 4 cut(s) 432, 477, 822, 1276
MslI CAYNNNNRTG 4 cut(s) 237, 807, 927, 1267
MspI CCGG 2 cut(s) 543, 575
MspR9I CCNGG 1 cut(s) 575
MwoI GCNNNNNNNGC 3 cut(s) 923, 932, 1043
NaeI GCCGGC 1 cut(s) 544
NciI CCSGG 1 cut(s) 575
NdeII GATC 2 cut(s) 189, 1058
NgoMIV GCCGGC 1 cut(s) 542
NheI GCTAGC 1 cut(s) 946
NlaIII CATG 5 cut(s) 148, 499, 861, 926, 1234
NlaIV GGNNCC 2 cut(s) 1071, 1097
NmuCI GTSAC 2 cut(s) 451, 627
NsbI TGCGCA 1 cut(s) 110
NspI RCATGY 2 cut(s) 861, 1234
NspV TTCGAA 1 cut(s) 907
OliI CACNNNNGTG 1 cut(s) 807
PaqCI CACCTGC 1 cut(s) 917
PciSI GCTCTTC 1 cut(s) 219
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 414, 727
PdiI GCCGGC 1 cut(s) 544
PfeI GAWTC 4 cut(s) 124, 175, 363, 1168
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 4 cut(s) 231, 814, 934, 968
PleI GAGTC 2 cut(s) 670, 856
PpsI GAGTC 2 cut(s) 670, 856
PsiI TTATAA 1 cut(s) 986
Psp124BI GAGCTC 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 694
PspEI GGTNACC 1 cut(s) 627
PspN4I GGNNCC 2 cut(s) 1071, 1097
PspPI GGNCC 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 1 cut(s) 1058
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 3 cut(s) 420, 1127, 1229
RsaNI GTAC 3 cut(s) 419, 1126, 1228
RseI CAYNNNNRTG 4 cut(s) 237, 807, 927, 1267
SacI GAGCTC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 4 cut(s) 432, 477, 822, 1276
SatI GCNGC 4 cut(s) 230, 813, 933, 967
Sau3AI GATC 2 cut(s) 189, 1058
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 2 cut(s) 670, 857
ScrFI CCNGG 1 cut(s) 575
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 5 cut(s) 37, 451, 586, 900, 933
SfcI CTRYAG 1 cut(s) 535
SfuI TTCGAA 1 cut(s) 907
SinI GGWCC 1 cut(s) 392
SmiMI CAYNNNNRTG 4 cut(s) 237, 807, 927, 1267
Sse9I AATT 5 cut(s) 566, 622, 657, 670, 909
SsiI CCGC 2 cut(s) 230, 680
SspMI CTAG 4 cut(s) 128, 356, 465, 947
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 573
TaaI ACNGT 6 cut(s) 444, 539, 834, 1008, 1193, 1255
TaiI ACGT 2 cut(s) 269, 697
TaqI TCGA 5 cut(s) 31, 152, 192, 907, 1032
TasI AATT 5 cut(s) 566, 622, 657, 670, 909
TatI WGTACW 1 cut(s) 1227
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 4 cut(s) 124, 175, 363, 1168
Tru1I TTAA 4 cut(s) 432, 477, 822, 1276
Tru9I TTAA 4 cut(s) 432, 477, 822, 1276
TscAI CASTG 3 cut(s) 226, 544, 616
TseFI GTSAC 2 cut(s) 451, 627
TseI GCWGC 3 cut(s) 812, 932, 966
Tsp45I GTSAC 2 cut(s) 451, 627
TspDTI ATGAA 7 cut(s) 255, 306, 335, 351, 484, 542, 660
TspGWI ACGGA 2 cut(s) 787, 868
TspRI CASTG 3 cut(s) 226, 544, 616
Tth111I GACNNNGTC 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 3 cut(s) 566, 657, 909
XceI RCATGY 2 cut(s) 861, 1234
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 4 cut(s) 128, 356, 465, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.