Rw6G029160

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0018627
Physical Location & Seq
Reverse (-)
53280337 .. 53283781
3445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G029160.1

Sequence Viewer

Length: 966 bp
ATGGAGGCCATACTGAATGAGACTGAAGACGAAGATGAGGAGAAGTTACTAGAGCGATTCTTATCAAAGGAGGAGGGTGATGATGGTCCACAGCGTAATCTCGCAGACCTCATACTTGCAACCATTAAGAACAAACAGTATGATGTCACCTCAGATGCTAGGTCTTTGGTTAAAATGGATACAGATTGCATAGAAGTCTACAAGGGGGTTGCAAAGTTTATGAAAAGCTACAGTGCCGGCAAGATTGCCAAACCATTCAAATTTATCCCTGCAATGCAACAATGGGAGGATGCCCTCTTTTCACTGAGCCTGATAATTGGTCAGTGGTCACCAACAGCTATGTATGAAGCTACAAGAATTCTTGCTTCAAATTTGACTCCGCAAAAAGCAGAACGTTTCTACAAGCATGTCTTGCTCCCACGGGTGAGACAAGACATTCGGAATAAGAAGAAGAAGAAGCCCTTGCATTTTTCCCTGTATCAATCTCTAAGGAAGTCCGTCCACAAGCGTGCTGCCTTTTTTAAAGGAATACTGTTGCCTTTGTGTGAGTCAAGGACATGCACTCTAAAAGAAGCCTCCGTTGTTGGGAGCATCATACAAAAGTTCGAATTTCTGCAAGTCATGCAGGGTATTTATTTTATAAAGCTTCTTTTGGAGAAACAGTATGCTTTGCCAAATCCTGTAATCGAGGCTCTTGTTGCACATTTTATGAGATCTGTGGAGGAACCAAGAGTAATGCCTGTGATATGGCACCAGTCTCTTCTTGCATTTGTGCAAAGGTACAAAAATGATATAACAAAGGACAACAAAGATAAGCTTAGGAATCTTCTTGAAGCACAAAACCACCATGAGATTACTCCTGAAATAAGAAGGGAGCTATTTCATAGCTGCAATGATGGTGAGAAGGAGCATGATCGTGCGTCAAACATGTTTGATATTCCAGAGGTGCCTCTGGAGGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

321

Amino Acids

37.3

Weight (kDa)

7.68

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 30 - 301 1e-88 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018627)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0291881
rosa_multiflora Rmu_sc0002484.1_g000003
rosa_roxburghii Rroxscaffold_7G00175660
rosa_rugosa Rorug06G0221700 Rorug06G0221700
rosa_samantha Rh6AG335100 Rh6BG342500 Rh6CG348600 Rh6DG335300
rosa_wichuraiana Rw6G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 641
AccB1I GGYRCC 2 cut(s) 750, 946
AccI GTMKAC 1 cut(s) 198
AciI CCGC 1 cut(s) 380
AclI AACGTT 1 cut(s) 394
AcsI RAATTY 4 cut(s) 260, 357, 370, 608
AcuI CTGAAG 1 cut(s) 45
AfaI GTAC 1 cut(s) 782
AfiI CCNNNNNNNGG 1 cut(s) 585
AflIII ACRYGT 1 cut(s) 927
AgsI TTSAA 3 cut(s) 259, 369, 833
AleI CACNNNNGTG 1 cut(s) 507
AluBI AGCT 7 cut(s) 228, 338, 350, 646, 817, 877, 888
AluI AGCT 7 cut(s) 228, 338, 350, 646, 817, 877, 888
Alw26I GTCTC 3 cut(s) 14, 421, 762
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 512, 888
ApoI RAATTY 4 cut(s) 260, 357, 370, 608
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 5 cut(s) 89, 139, 321, 436, 911
AsuII TTCGAA 1 cut(s) 606
AvaII GGWCC 1 cut(s) 86
BanI GGYRCC 2 cut(s) 750, 946
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 499, 875
BccI CCATC 2 cut(s) 77, 890
BciVI GTATCC 1 cut(s) 172
BcoDI GTCTC 3 cut(s) 14, 421, 762
BfaI CTAG 2 cut(s) 50, 159
BfmI CTRYAG 1 cut(s) 229
BfuI GTATCC 1 cut(s) 172
BglII AGATCT 1 cut(s) 713
BisI GCNGC 2 cut(s) 513, 889
BlsI GCNGC 2 cut(s) 514, 890
Bme18I GGWCC 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 3 cut(s) 726, 752, 948
BmsI GCATC 3 cut(s) 145, 280, 600
BpiI GAAGAC 1 cut(s) 33
Bpu10I CCTNAGC 1 cut(s) 818
Bpu14I TTCGAA 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 419
BsaXI ACNNNNNCTCC 2 cut(s) 647, 677
Bsc4I CCNNNNNNNGG 1 cut(s) 585
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 754
Bse3DI GCAATG 2 cut(s) 279, 898
Bse8I GATNNNNATC 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 2 cut(s) 295, 964
BseJI GATNNNNATC 1 cut(s) 61
BseLI CCNNNNNNNGG 1 cut(s) 585
BseMI GCAATG 2 cut(s) 279, 898
BseMII CTCAG 2 cut(s) 165, 296
BseNI ACTGG 1 cut(s) 754
BseRI GAGGAG 2 cut(s) 53, 86
BseXI GCAGC 2 cut(s) 499, 875
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 2 cut(s) 750, 946
BsiSI CCGG 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 585
BsmAI GTCTC 3 cut(s) 14, 421, 762
BsnI GGCC 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 606
Bsp143I GATC 2 cut(s) 713, 913
BspACI CCGC 1 cut(s) 380
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 2 cut(s) 164, 297
BspLI GGNNCC 3 cut(s) 726, 752, 948
BspT104I TTCGAA 1 cut(s) 606
BspT107I GGYRCC 2 cut(s) 750, 946
BsrDI GCAATG 2 cut(s) 279, 898
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 1 cut(s) 754
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 2 cut(s) 713, 913
Bst4CI ACNGT 4 cut(s) 138, 233, 534, 663
Bst6I CTCTTC 1 cut(s) 765
BstAPI GCANNNNNTGC 2 cut(s) 412, 622
BstBI TTCGAA 1 cut(s) 606
BstC8I GCNNGC 2 cut(s) 238, 510
BstDEI CTNAG 4 cut(s) 151, 305, 488, 818
BstDSI CCRYGG 1 cut(s) 419
BstEII GGTNACC 1 cut(s) 327
BstF5I GGATG 2 cut(s) 295, 964
BstKTI GATC 2 cut(s) 716, 916
BstMAI GTCTC 3 cut(s) 14, 421, 762
BstMBI GATC 2 cut(s) 713, 913
BstMWI GCNNNNNNNGC 3 cut(s) 412, 622, 698
BstNSI RCATGY 3 cut(s) 410, 561, 931
BstPI GGTNACC 1 cut(s) 327
BstSFI CTRYAG 1 cut(s) 229
BstV1I GCAGC 2 cut(s) 499, 875
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 713
BstYI RGATCY 1 cut(s) 713
BsuI GTATCC 1 cut(s) 172
BsuRI GGCC 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 419
BtsCI GGATG 2 cut(s) 295, 964
BtsIMutI CAGTG 3 cut(s) 238, 302, 329
Cac8I GCNNGC 2 cut(s) 238, 510
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 86
CseI GACGC 1 cut(s) 909
Csp6I GTAC 1 cut(s) 781
CviAII CATG 6 cut(s) 407, 558, 622, 848, 911, 928
CviQI GTAC 1 cut(s) 781
DdeI CTNAG 4 cut(s) 151, 305, 488, 818
DpnI GATC 2 cut(s) 715, 915
DpnII GATC 2 cut(s) 713, 913
DraI TTTAAA 1 cut(s) 523
Eam1104I CTCTTC 1 cut(s) 765
EarI CTCTTC 1 cut(s) 765
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 45
Eco91I GGTNACC 1 cut(s) 327
EcoO65I GGTNACC 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 357
FaeI CATG 6 cut(s) 410, 561, 625, 851, 914, 931
FalI AAGNNNNNCTT 6 cut(s) 395, 427, 446, 478, 801, 833
FatI CATG 6 cut(s) 406, 557, 621, 847, 910, 927
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 2 cut(s) 513, 889
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 2 cut(s) 513, 889
FspBI CTAG 2 cut(s) 50, 159
GluI GCNGC 2 cut(s) 513, 889
HaeIII GGCC 1 cut(s) 8
HapII CCGG 1 cut(s) 237
HgaI GACGC 1 cut(s) 909
Hin1II CATG 6 cut(s) 410, 561, 625, 851, 914, 931
HindIII AAGCTT 2 cut(s) 644, 815
HinfI GANTC 4 cut(s) 57, 376, 548, 823
HpaII CCGG 1 cut(s) 237
HphI GGTGA 5 cut(s) 89, 139, 321, 436, 911
Hpy166II GTNNAC 3 cut(s) 89, 199, 502
Hpy188I TCNGA 2 cut(s) 154, 441
Hpy188III TCNNGA 4 cut(s) 830, 860, 941, 953
Hpy8I GTNNAC 3 cut(s) 89, 199, 502
HpyAV CCTTC 2 cut(s) 864, 898
HpyCH4III ACNGT 4 cut(s) 138, 233, 534, 663
HpyCH4IV ACGT 1 cut(s) 394
HpyF10VI GCNNNNNNNGC 3 cut(s) 412, 622, 698
HpyF3I CTNAG 4 cut(s) 151, 305, 488, 818
HpySE526I ACGT 1 cut(s) 394
Hsp92II CATG 6 cut(s) 410, 561, 625, 851, 914, 931
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Kzo9I GATC 2 cut(s) 713, 913
LmnI GCTCC 4 cut(s) 420, 588, 874, 907
Lsp1109I GCAGC 2 cut(s) 499, 875
LweI GCATC 3 cut(s) 145, 280, 600
MaeI CTAG 2 cut(s) 50, 159
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 3 cut(s) 45, 145, 327
MalI GATC 2 cut(s) 715, 915
MboI GATC 2 cut(s) 713, 913
MboII GAAGA 7 cut(s) 38, 44, 460, 463, 466, 752, 818
MflI RGATCY 1 cut(s) 713
MluCI AATT 5 cut(s) 260, 315, 357, 370, 608
MlyI GAGTC 2 cut(s) 370, 557
MroNI GCCGGC 1 cut(s) 236
MseI TTAA 3 cut(s) 126, 171, 522
MslI CAYNNNNRTG 2 cut(s) 507, 915
MspI CCGG 1 cut(s) 237
MwoI GCNNNNNNNGC 3 cut(s) 412, 622, 698
NaeI GCCGGC 1 cut(s) 238
NdeII GATC 2 cut(s) 713, 913
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 6 cut(s) 410, 561, 625, 851, 914, 931
NlaIV GGNNCC 3 cut(s) 726, 752, 948
NmuCI GTSAC 2 cut(s) 145, 327
NspI RCATGY 3 cut(s) 410, 561, 931
NspV TTCGAA 1 cut(s) 606
OliI CACNNNNGTG 1 cut(s) 507
PciI ACATGT 1 cut(s) 927
PdiI GCCGGC 1 cut(s) 238
PfeI GAWTC 2 cut(s) 57, 823
PkrI GCNGC 2 cut(s) 514, 890
PleI GAGTC 2 cut(s) 370, 556
PpsI GAGTC 2 cut(s) 370, 556
PscI ACATGT 1 cut(s) 927
PsiI TTATAA 1 cut(s) 641
Psp1406I AACGTT 1 cut(s) 394
PspEI GGTNACC 1 cut(s) 327
PspN4I GGNNCC 3 cut(s) 726, 752, 948
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 1 cut(s) 713
RsaI GTAC 1 cut(s) 782
RsaNI GTAC 1 cut(s) 781
RseI CAYNNNNRTG 2 cut(s) 507, 915
SaqAI TTAA 3 cut(s) 126, 171, 522
SatI GCNGC 2 cut(s) 513, 889
Sau3AI GATC 2 cut(s) 713, 913
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 2 cut(s) 370, 557
SfaNI GCATC 3 cut(s) 145, 280, 600
SfcI CTRYAG 1 cut(s) 229
SfuI TTCGAA 1 cut(s) 606
SinI GGWCC 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 507, 915
Sse9I AATT 5 cut(s) 260, 315, 357, 370, 608
SsiI CCGC 1 cut(s) 380
SspMI CTAG 2 cut(s) 50, 159
TaaI ACNGT 4 cut(s) 138, 233, 534, 663
TaiI ACGT 1 cut(s) 397
TaqI TCGA 2 cut(s) 606, 687
TasI AATT 5 cut(s) 260, 315, 357, 370, 608
TfiI GAWTC 2 cut(s) 57, 823
Tru1I TTAA 3 cut(s) 126, 171, 522
Tru9I TTAA 3 cut(s) 126, 171, 522
TscAI CASTG 3 cut(s) 238, 309, 329
TseFI GTSAC 2 cut(s) 145, 327
TseI GCWGC 2 cut(s) 512, 888
Tsp45I GTSAC 2 cut(s) 145, 327
TspDTI ATGAA 3 cut(s) 236, 360, 872
TspGWI ACGGA 2 cut(s) 487, 568
TspRI CASTG 3 cut(s) 238, 309, 329
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 4 cut(s) 260, 357, 370, 608
XceI RCATGY 3 cut(s) 410, 561, 931
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 2 cut(s) 50, 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.