Rroxscaffold_7G00175660

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0018627
Physical Location & Seq
Forward (+)
15146003 .. 15155452
9450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00175660.1

Sequence Viewer

Length: 1398 bp
ATGACGAAGAAGAGGGGGAGAGAGAGGCATCGGAATCCAAAGCCGTTCCTCGCCGAAGACGATGTCTCTGTGGCTTCGACAAAGCTAACCAAACGGCCCAAAATGGCTCACAAGCGCCAAGAGAATCTAGTTTTATCAGCAGGCATGAGCTCGAAAATCCTCAGGGAAGCTCAGATTCAGGAGAAGGAGATCGAGGAAGAGGAGAACACAAACGCCTTCACTGAAGGGCCGCTCAACGATGATTTTGATACGAAAAACTCAGATCTCAAAAGTCATCATGAGGAGTCGAACCCCAATTATCAAGATAAAGCAAAGACCACACTAACACAACCAACCAGTTACAAACTCGCAAAGTTGATATATATTACACTAAGCATACTGGAACTCATAGCTTCAGAGGCCCCCCGAAGCTGGTGGCCCGAGGTGCTGCCTCGGTTAGCTACCCTCGGGGCGGCTCGTGATGATGGTCCACAGCGTAATCTCGCAGACCTCATACTTGCAACCATTAAGAACAAACAGTATGATGTCACGTCAGATGCTAGTTCTTTGGTTAAAATGGATACAGATTTCATAGAAGTCTACAAGGGGGTTGCAAAGTTTATGAAAAGCTACAGTGCCGGCAAGATTGCCAAACCATTCAAATTTATCCCTGCAATAATGCAACAATGGGAGGATGCCCTCTATTTCACTGAGCCTGATAATTGGTCACCAACTGCTATGTATGAAGCAACAAGAATTCTTGCTTCTAATTTGACTCCGCAAAAAGCAGAACGTTTCTACAAGCATGTCTTGCTCCCACGGGTGAGACAAGACATTCGGAATAAGAAGAAGAAGAAGCCCTTGCATTTTTCCCTGTATCGATCTCTAAGGAAGTCCGTCCACAAGCGTGCTGCCTTTTTTAAAGGAATACTGTTGCCTTTGTGTGAGAAGCTAGCCTCCGTTGTTGGGAGCATCATACAAAAGGTTCGAATTTCTGCAAGTCATGCAGGTGCTGCAATAATGATGCTAGCAGAGATGGAATATAGCAGCGCAAGAAGTTATTTTATAAAGCTTCTTTTGGAGAAACAATATGCTTTGCCAAATCCTGTAATCGAGGCTGTTGTTGCACATTTTATGAGATCTGTGGAGGAACCAAGATTGGTGTGCATAATCCCTTGCAGGTACAAAAATGATATAACAAAAGACGACAAAGATAAGCTTAGGAATCTTCTTGAAGCACAAAACCACCGTGAGATTACTCCTGAAATAAGAAGGGAGTTATTTCATAGCTGCAATCATGGTGAGAAGGAGCATGATCGTGCGTCAACTGATCAGGTCTCAAGATTTTCAATATTTGTATATATTTCCTATATTCCTGGGGTTTCTTGGACTGCAAGTTCCTCCGCTAGGACTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

465

Amino Acids

53.26

Weight (kDa)

9.45

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 157 - 429 6.2e-88 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018627)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0291881
rosa_multiflora Rmu_sc0002484.1_g000003
rosa_roxburghii Rroxscaffold_7G00175660
rosa_rugosa Rorug06G0221700 Rorug06G0221700
rosa_samantha Rh6AG335100 Rh6BG342500 Rh6CG348600 Rh6DG335300
rosa_wichuraiana Rw6G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1046
AarI CACCTGC 1 cut(s) 977
Acc36I ACCTGC 2 cut(s) 977, 1149
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 579
AciI CCGC 4 cut(s) 230, 452, 758, 1383
AclI AACGTT 1 cut(s) 772
AcsI RAATTY 3 cut(s) 641, 735, 969
AcuI CTGAAG 2 cut(s) 243, 378
AfaI GTAC 1 cut(s) 1163
AfiI CCNNNNNNNGG 4 cut(s) 411, 451, 945, 1386
AgsI TTSAA 3 cut(s) 640, 1214, 1329
AjiI CACGTC 1 cut(s) 531
AjnI CCWGG 1 cut(s) 1354
AleI CACNNNNGTG 1 cut(s) 885
AloI GAACNNNNNNTCC 2 cut(s) 1360, 1392
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 3 cut(s) 70, 799, 1321
AlwNI CAGNNNCTG 1 cut(s) 992
Ama87I CYCGRG 2 cut(s) 419, 446
AoxI GGCC 4 cut(s) 95, 227, 399, 416
ApeKI GCWGC 5 cut(s) 427, 890, 992, 1026, 1269
ApoI RAATTY 3 cut(s) 641, 735, 969
ArsI GACNNNNNNTTYG 4 cut(s) 48, 80, 798, 830
AspLEI GCGC 2 cut(s) 117, 1031
AspS9I GGNCC 5 cut(s) 96, 227, 400, 417, 467
AsuHPI GGTGA 3 cut(s) 699, 814, 1292
AsuII TTCGAA 1 cut(s) 967
AsuNHI GCTAGC 2 cut(s) 931, 1006
AvaI CYCGRG 2 cut(s) 419, 446
AvaII GGWCC 1 cut(s) 467
AxyI CCTNAGG 1 cut(s) 161
BanII GRGCYC 1 cut(s) 152
BauI CACGAG 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 5 cut(s) 414, 877, 979, 1038, 1256
BccI CCATC 2 cut(s) 458, 1009
BceAI ACGGC 2 cut(s) 28, 110
BciT130I CCWGG 1 cut(s) 1356
BciVI GTATCC 1 cut(s) 553
BclI TGATCA 1 cut(s) 1309
BcoDI GTCTC 3 cut(s) 70, 799, 1321
BfaI CTAG 5 cut(s) 128, 540, 932, 1007, 1386
BfmI CTRYAG 1 cut(s) 610
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 2 cut(s) 977, 1149
BfuI GTATCC 1 cut(s) 553
BglII AGATCT 2 cut(s) 262, 1118
BisI GCNGC 7 cut(s) 230, 428, 453, 891, 993, 1027, 1270
BlsI GCNGC 7 cut(s) 231, 429, 454, 892, 994, 1028, 1271
Bme1390I CCNGG 1 cut(s) 1356
Bme18I GGWCC 1 cut(s) 467
BmeT110I CYCGRG 2 cut(s) 419, 446
BmgBI CACGTC 1 cut(s) 531
BmgT120I GGNCC 5 cut(s) 96, 227, 400, 417, 467
BmiI GGNNCC 2 cut(s) 402, 1131
BmrFI CCNGG 1 cut(s) 1356
BmsI GCATC 5 cut(s) 37, 526, 664, 960, 993
BmtI GCTAGC 2 cut(s) 935, 1010
BpiI GAAGAC 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 1199
Bpu14I TTCGAA 1 cut(s) 967
BpuEI CTTGAG 1 cut(s) 1303
Bsa29I ATCGAT 1 cut(s) 859
BsaI GGTCTC 1 cut(s) 1321
BsaJI CCNNGG 5 cut(s) 420, 431, 445, 797, 1355
Bsc4I CCNNNNNNNGG 4 cut(s) 411, 451, 945, 1386
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 2 cut(s) 336, 384
Bse21I CCTNAGG 1 cut(s) 161
BseBI CCWGG 1 cut(s) 1356
BseCI ATCGAT 1 cut(s) 859
BseDI CCNNGG 5 cut(s) 420, 431, 445, 797, 1355
BseGI GGATG 1 cut(s) 679
BseLI CCNNNNNNNGG 4 cut(s) 411, 451, 945, 1386
BseMII CTCAG 4 cut(s) 175, 185, 273, 681
BseNI ACTGG 2 cut(s) 336, 384
BseRI GAGGAG 2 cut(s) 215, 296
BseXI GCAGC 5 cut(s) 414, 877, 979, 1038, 1256
BshFI GGCC 4 cut(s) 97, 229, 401, 418
BshVI ATCGAT 1 cut(s) 859
BsiHKAI GWGCWC 1 cut(s) 152
BsiHKCI CYCGRG 2 cut(s) 419, 446
BsiSI CCGG 1 cut(s) 618
BslI CCNNNNNNNGG 4 cut(s) 411, 451, 945, 1386
BsmAI GTCTC 3 cut(s) 70, 799, 1321
BsnI GGCC 4 cut(s) 97, 229, 401, 418
Bso31I GGTCTC 1 cut(s) 1321
BsoBI CYCGRG 2 cut(s) 419, 446
Bsp119I TTCGAA 1 cut(s) 967
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 6 cut(s) 189, 262, 860, 1118, 1294, 1309
BspACI CCGC 4 cut(s) 230, 452, 758, 1383
BspANI GGCC 4 cut(s) 97, 229, 401, 418
BspCNI CTCAG 4 cut(s) 174, 184, 272, 682
BspDI ATCGAT 1 cut(s) 859
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 2 cut(s) 402, 1131
BspMI ACCTGC 2 cut(s) 977, 1149
BspOI GCTAGC 2 cut(s) 935, 1010
BspT104I TTCGAA 1 cut(s) 967
BspTNI GGTCTC 1 cut(s) 1321
BsrBI CCGCTC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 2 cut(s) 336, 384
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 5 cut(s) 420, 431, 445, 797, 1355
BssMI GATC 6 cut(s) 189, 262, 860, 1118, 1294, 1309
BssSI CACGAG 1 cut(s) 456
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 1356
Bst4CI ACNGT 4 cut(s) 519, 614, 912, 1229
Bst6I CTCTTC 2 cut(s) 5, 192
BstAPI GCANNNNNTGC 3 cut(s) 790, 983, 992
BstBI TTCGAA 1 cut(s) 967
BstC8I GCNNGC 5 cut(s) 142, 619, 888, 933, 1008
BstDEI CTNAG 7 cut(s) 161, 171, 259, 371, 690, 866, 1199
BstDSI CCRYGG 1 cut(s) 797
BstEII GGTNACC 1 cut(s) 705
BstENI CCTNNNNNAGG 1 cut(s) 1384
BstF5I GGATG 1 cut(s) 679
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 117, 1031
BstKTI GATC 6 cut(s) 192, 265, 863, 1121, 1297, 1312
BstMAI GTCTC 3 cut(s) 70, 799, 1321
BstMBI GATC 6 cut(s) 189, 262, 860, 1118, 1294, 1309
BstMWI GCNNNNNNNGC 6 cut(s) 398, 424, 790, 983, 992, 1103
BstNI CCWGG 1 cut(s) 1356
BstNSI RCATGY 1 cut(s) 788
BstPI GGTNACC 1 cut(s) 705
BstSCI CCNGG 1 cut(s) 1354
BstSFI CTRYAG 1 cut(s) 610
BstV1I GCAGC 5 cut(s) 414, 877, 979, 1038, 1256
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 2 cut(s) 262, 1118
BstYI RGATCY 2 cut(s) 262, 1118
Bsu15I ATCGAT 1 cut(s) 859
Bsu36I CCTNAGG 1 cut(s) 161
BsuI GTATCC 1 cut(s) 553
BsuRI GGCC 4 cut(s) 97, 229, 401, 418
BsuTUI ATCGAT 1 cut(s) 859
BtgI CCRYGG 1 cut(s) 797
BtrI CACGTC 1 cut(s) 531
BtsCI GGATG 1 cut(s) 679
BtsIMutI CAGTG 3 cut(s) 219, 619, 687
BveI ACCTGC 2 cut(s) 977, 1149
Cac8I GCNNGC 5 cut(s) 142, 619, 888, 933, 1008
CaiI CAGNNNCTG 1 cut(s) 992
CciI TCATGA 1 cut(s) 277
CfoI GCGC 2 cut(s) 117, 1031
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 5 cut(s) 96, 227, 400, 417, 467
ClaI ATCGAT 1 cut(s) 859
CseI GACGC 1 cut(s) 1290
Csp6I GTAC 1 cut(s) 1162
CviAII CATG 6 cut(s) 145, 278, 785, 983, 1277, 1292
CviQI GTAC 1 cut(s) 1162
DdeI CTNAG 7 cut(s) 161, 171, 259, 371, 690, 866, 1199
DpnI GATC 6 cut(s) 191, 264, 862, 1120, 1296, 1311
DpnII GATC 6 cut(s) 189, 262, 860, 1118, 1294, 1309
DraI TTTAAA 1 cut(s) 901
Eam1104I CTCTTC 2 cut(s) 5, 192
EarI CTCTTC 2 cut(s) 5, 192
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco31I GGTCTC 1 cut(s) 1321
Eco47I GGWCC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 243, 378
Eco81I CCTNAGG 1 cut(s) 161
Eco88I CYCGRG 2 cut(s) 419, 446
Eco91I GGTNACC 1 cut(s) 705
EcoICRI GAGCTC 1 cut(s) 150
EcoNI CCTNNNNNAGG 1 cut(s) 1384
EcoO109I RGGNCCY 1 cut(s) 400
EcoO65I GGTNACC 1 cut(s) 705
EcoRI GAATTC 1 cut(s) 735
EcoRII CCWGG 1 cut(s) 1354
EcoT38I GRGCYC 1 cut(s) 152
FaeI CATG 6 cut(s) 148, 281, 788, 986, 1280, 1295
FalI AAGNNNNNCTT 6 cut(s) 773, 805, 824, 856, 1182, 1214
FatI CATG 6 cut(s) 144, 277, 784, 982, 1276, 1291
FbaI TGATCA 1 cut(s) 1309
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 7 cut(s) 230, 428, 453, 891, 993, 1027, 1270
FokI GGATG 1 cut(s) 686
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 7 cut(s) 230, 428, 453, 891, 993, 1027, 1270
FspBI CTAG 5 cut(s) 128, 540, 932, 1007, 1386
GlaI GCGC 2 cut(s) 116, 1030
GluI GCNGC 7 cut(s) 230, 428, 453, 891, 993, 1027, 1270
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 4 cut(s) 97, 229, 401, 418
HapII CCGG 1 cut(s) 618
HgaI GACGC 1 cut(s) 1290
HhaI GCGC 2 cut(s) 117, 1031
Hin1II CATG 6 cut(s) 148, 281, 788, 986, 1280, 1295
Hin6I GCGC 2 cut(s) 115, 1029
HinP1I GCGC 2 cut(s) 115, 1029
HincII GTYRAC 1 cut(s) 1305
HindII GTYRAC 1 cut(s) 1305
HindIII AAGCTT 2 cut(s) 1049, 1196
HinfI GANTC 7 cut(s) 34, 124, 175, 284, 754, 1204, 1390
HpaII CCGG 1 cut(s) 618
HphI GGTGA 3 cut(s) 699, 814, 1292
Hpy166II GTNNAC 4 cut(s) 470, 580, 880, 1305
Hpy188I TCNGA 6 cut(s) 33, 174, 262, 397, 535, 819
Hpy188III TCNNGA 7 cut(s) 179, 278, 302, 458, 1211, 1241, 1320
Hpy8I GTNNAC 4 cut(s) 470, 580, 880, 1305
HpyAV CCTTC 5 cut(s) 178, 218, 226, 1245, 1279
HpyCH4III ACNGT 4 cut(s) 519, 614, 912, 1229
HpyCH4IV ACGT 2 cut(s) 530, 772
HpyF10VI GCNNNNNNNGC 6 cut(s) 398, 424, 790, 983, 992, 1103
HpyF3I CTNAG 7 cut(s) 161, 171, 259, 371, 690, 866, 1199
HpySE526I ACGT 2 cut(s) 530, 772
Hsp92II CATG 6 cut(s) 148, 281, 788, 986, 1280, 1295
HspAI GCGC 2 cut(s) 115, 1029
KroI GCCGGC 1 cut(s) 617
KroNI GCCGGC 1 cut(s) 619
Ksp22I TGATCA 1 cut(s) 1309
Kzo9I GATC 6 cut(s) 189, 262, 860, 1118, 1294, 1309
LmnI GCTCC 3 cut(s) 798, 948, 1288
Lsp1109I GCAGC 5 cut(s) 414, 877, 979, 1038, 1256
LweI GCATC 5 cut(s) 37, 526, 664, 960, 993
MaeI CTAG 5 cut(s) 128, 540, 932, 1007, 1386
MaeII ACGT 2 cut(s) 530, 772
MaeIII GTNAC 3 cut(s) 338, 526, 705
MalI GATC 6 cut(s) 191, 264, 862, 1120, 1296, 1311
MbiI CCGCTC 1 cut(s) 232
MboI GATC 6 cut(s) 189, 262, 860, 1118, 1294, 1309
MboII GAAGA 8 cut(s) 19, 22, 68, 209, 838, 841, 844, 1199
MflI RGATCY 2 cut(s) 262, 1118
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 6 cut(s) 295, 641, 700, 735, 748, 969
MlyI GAGTC 3 cut(s) 293, 748, 1384
MroNI GCCGGC 1 cut(s) 617
MseI TTAA 3 cut(s) 507, 552, 900
MslI CAYNNNNRTG 3 cut(s) 885, 987, 1296
MspI CCGG 1 cut(s) 618
MspR9I CCNGG 1 cut(s) 1356
MvaI CCWGG 1 cut(s) 1356
MwoI GCNNNNNNNGC 6 cut(s) 398, 424, 790, 983, 992, 1103
NaeI GCCGGC 1 cut(s) 619
NdeII GATC 6 cut(s) 189, 262, 860, 1118, 1294, 1309
NgoMIV GCCGGC 1 cut(s) 617
NheI GCTAGC 2 cut(s) 931, 1006
NlaIII CATG 6 cut(s) 148, 281, 788, 986, 1280, 1295
NlaIV GGNNCC 2 cut(s) 402, 1131
NmuCI GTSAC 2 cut(s) 526, 705
NspI RCATGY 1 cut(s) 788
NspV TTCGAA 1 cut(s) 967
OliI CACNNNNGTG 1 cut(s) 885
PagI TCATGA 1 cut(s) 277
PaqCI CACCTGC 1 cut(s) 977
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PdiI GCCGGC 1 cut(s) 619
PfeI GAWTC 4 cut(s) 34, 124, 175, 1204
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 7 cut(s) 231, 429, 454, 892, 994, 1028, 1271
PleI GAGTC 3 cut(s) 292, 748, 1384
PpsI GAGTC 3 cut(s) 292, 748, 1384
PsiI TTATAA 1 cut(s) 1046
Psp124BI GAGCTC 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 772
Psp6I CCWGG 1 cut(s) 1354
PspEI GGTNACC 1 cut(s) 705
PspGI CCWGG 1 cut(s) 1354
PspN4I GGNNCC 2 cut(s) 402, 1131
PspPI GGNCC 5 cut(s) 96, 227, 400, 417, 467
PstNI CAGNNNCTG 1 cut(s) 992
PsuI RGATCY 2 cut(s) 262, 1118
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 1 cut(s) 1163
RsaNI GTAC 1 cut(s) 1162
RseI CAYNNNNRTG 3 cut(s) 885, 987, 1296
SacI GAGCTC 1 cut(s) 152
SaqAI TTAA 3 cut(s) 507, 552, 900
SatI GCNGC 7 cut(s) 230, 428, 453, 891, 993, 1027, 1270
Sau3AI GATC 6 cut(s) 189, 262, 860, 1118, 1294, 1309
Sau96I GGNCC 5 cut(s) 96, 227, 400, 417, 467
SchI GAGTC 3 cut(s) 293, 748, 1384
ScrFI CCNGG 1 cut(s) 1356
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 5 cut(s) 37, 526, 664, 960, 993
SfcI CTRYAG 1 cut(s) 610
SfuI TTCGAA 1 cut(s) 967
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 3 cut(s) 885, 987, 1296
SmlI CTYRAG 1 cut(s) 1318
SmoI CTYRAG 1 cut(s) 1318
Sse9I AATT 6 cut(s) 295, 641, 700, 735, 748, 969
SsiI CCGC 4 cut(s) 230, 452, 758, 1383
SspI AATATT 1 cut(s) 1332
SspMI CTAG 5 cut(s) 128, 540, 932, 1007, 1386
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 1354
TaaI ACNGT 4 cut(s) 519, 614, 912, 1229
TaiI ACGT 2 cut(s) 533, 775
TaqI TCGA 7 cut(s) 77, 152, 192, 287, 859, 967, 1092
TasI AATT 6 cut(s) 295, 641, 700, 735, 748, 969
TauI GCSGC 2 cut(s) 232, 455
TfiI GAWTC 4 cut(s) 34, 124, 175, 1204
Tru1I TTAA 3 cut(s) 507, 552, 900
Tru9I TTAA 3 cut(s) 507, 552, 900
TscAI CASTG 3 cut(s) 226, 619, 694
TseFI GTSAC 2 cut(s) 526, 705
TseI GCWGC 5 cut(s) 427, 890, 992, 1026, 1269
Tsp45I GTSAC 2 cut(s) 526, 705
TspDTI ATGAA 4 cut(s) 559, 617, 738, 1253
TspGWI ACGGA 2 cut(s) 865, 928
TspRI CASTG 3 cut(s) 226, 619, 694
Tth111I GACNNNGTC 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 467
XagI CCTNNNNNAGG 1 cut(s) 1384
XapI RAATTY 3 cut(s) 641, 735, 969
XceI RCATGY 1 cut(s) 788
XmiI GTMKAC 1 cut(s) 579
XspI CTAG 5 cut(s) 128, 540, 932, 1007, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.