Rmu_sc0002484.1_g000003

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002484.1
OG0018627
Physical Location & Seq
Reverse (-)
15290 .. 18524
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002484.1_g000003.1.cds

Sequence Viewer

Length: 921 bp
atgacgaagaagaaggggaaagagaggcatcggaatccaaagccgttcctcgccgaagacgatgtctctgtggcttctacacagctaaccaaacagcccaaaatggcgctccagcgccaagagaatctagttttatcagcaggcatgagctcgaaaatcctcagagaagctcagattcaggagaaggagatcgaggaagaggagaacacaaacgccttcactgaagagccgcacaacgatgaagactttgatacgttccttgaaacacatagagatgatgaatcaaacaaagtgatggcggccatactgaatgagactgaagacgaagatgaggagaagttactagagcgattcttatcaaaggaggagggtgatgatggtccacagcgtaatctcgcagacctcatacttgcaaccattaagaacaaacagtatgatgtcacctcagatgctaggtctttggttaaaatggatacagattgcatagaagtctacaagggggttgcaaagtttatgaaaagctacagtgccggcaagattgccaaaccattcaaatttatccctgcaatgcaacaatgggaggatgccctctatttcactgagcctgataattggtcaccaactgctatgtatgaagctacaagaattcttgcttcaaatttgactccgcaaaaagcagaacgtttctacaagcatgtcttgctcccacggcttcttttggagaaacaatatgctttgccaaatcctgtaatcgaggctcttgttgcacattttatgagatctgtggaggaaccaagagtaatgcctgtgatatggcaccagtctcttcttgcatttgtgcaaaggtacaaaaatgatatagcaaaggacaacaaagataagcataggaatcttcttgaagcacaaaccaccgtgaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

306

Amino Acids

35.43

Weight (kDa)

5.38

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018627)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0291881
rosa_multiflora Rmu_sc0002484.1_g000003
rosa_roxburghii Rroxscaffold_7G00175660
rosa_rugosa Rorug06G0221700 Rorug06G0221700
rosa_samantha Rh6AG335100 Rh6BG342500 Rh6CG348600 Rh6DG335300
rosa_wichuraiana Rw6G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 816
AccI GTMKAC 1 cut(s) 492
AciI CCGC 3 cut(s) 230, 299, 668
AclI AACGTT 1 cut(s) 682
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 3 cut(s) 554, 645, 658
AcuI CTGAAG 2 cut(s) 243, 339
AfaI GTAC 1 cut(s) 848
AfiI CCNNNNNNNGG 1 cut(s) 915
AgsI TTSAA 4 cut(s) 263, 553, 657, 899
AluBI AGCT 5 cut(s) 85, 150, 170, 522, 638
AluI AGCT 5 cut(s) 85, 150, 170, 522, 638
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 3 cut(s) 70, 308, 828
AoxI GGCC 1 cut(s) 300
ApoI RAATTY 3 cut(s) 554, 645, 658
ArsI GACNNNNNNTTYG 2 cut(s) 48, 80
AspLEI GCGC 2 cut(s) 109, 117
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 3 cut(s) 383, 433, 609
AvaII GGWCC 1 cut(s) 380
BanI GGYRCC 1 cut(s) 816
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 3 cut(s) 63, 249, 327
Bbv12I GWGCWC 1 cut(s) 152
BccI CCATC 2 cut(s) 289, 371
BceAI ACGGC 2 cut(s) 28, 725
BciVI GTATCC 1 cut(s) 466
BcoDI GTCTC 3 cut(s) 70, 308, 828
BfaI CTAG 3 cut(s) 128, 344, 453
BfmI CTRYAG 1 cut(s) 523
BfoI RGCGCY 2 cut(s) 110, 118
BfuI GTATCC 1 cut(s) 466
BglII AGATCT 1 cut(s) 779
BisI GCNGC 2 cut(s) 230, 300
BlsI GCNGC 2 cut(s) 231, 301
Bme18I GGWCC 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 2 cut(s) 792, 818
BmsI GCATC 3 cut(s) 37, 439, 574
BpiI GAAGAC 3 cut(s) 63, 249, 327
BpmI CTGGAG 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 355
BsaJI CCNNGG 1 cut(s) 707
Bsc4I CCNNNNNNNGG 1 cut(s) 915
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 820
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 355
BseDI CCNNGG 1 cut(s) 707
BseGI GGATG 1 cut(s) 589
BseJI GATNNNNATC 1 cut(s) 355
BseLI CCNNNNNNNGG 1 cut(s) 915
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 4 cut(s) 175, 185, 459, 591
BseNI ACTGG 1 cut(s) 820
BseRI GAGGAG 3 cut(s) 215, 347, 380
BshFI GGCC 1 cut(s) 302
BshNI GGYRCC 1 cut(s) 816
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 531
BslI CCNNNNNNNGG 1 cut(s) 915
BsmAI GTCTC 3 cut(s) 70, 308, 828
BsnI GGCC 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 2 cut(s) 189, 779
BspACI CCGC 3 cut(s) 230, 299, 668
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 4 cut(s) 174, 184, 458, 592
BspLI GGNNCC 2 cut(s) 792, 818
BspQI GCTCTTC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 816
BsrDI GCAATG 1 cut(s) 573
BsrFI RCCGGY 1 cut(s) 530
BsrI ACTGG 1 cut(s) 820
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 707
BssMI GATC 2 cut(s) 189, 779
Bst4CI ACNGT 3 cut(s) 432, 527, 913
Bst6I CTCTTC 3 cut(s) 192, 219, 831
BstAPI GCANNNNNTGC 1 cut(s) 700
BstC8I GCNNGC 2 cut(s) 142, 532
BstDEI CTNAG 4 cut(s) 161, 171, 445, 600
BstDSI CCRYGG 1 cut(s) 707
BstEII GGTNACC 1 cut(s) 615
BstF5I GGATG 1 cut(s) 589
BstH2I RGCGCY 2 cut(s) 110, 118
BstHHI GCGC 2 cut(s) 109, 117
BstKTI GATC 2 cut(s) 192, 782
BstMAI GTCTC 3 cut(s) 70, 308, 828
BstMBI GATC 2 cut(s) 189, 779
BstMWI GCNNNNNNNGC 3 cut(s) 700, 709, 764
BstNSI RCATGY 1 cut(s) 698
BstPI GGTNACC 1 cut(s) 615
BstSFI CTRYAG 1 cut(s) 523
BstV2I GAAGAC 3 cut(s) 63, 249, 327
BstX2I RGATCY 1 cut(s) 779
BstYI RGATCY 1 cut(s) 779
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 1 cut(s) 302
BtgI CCRYGG 1 cut(s) 707
BtsCI GGATG 1 cut(s) 589
BtsIMutI CAGTG 3 cut(s) 219, 532, 597
Cac8I GCNNGC 2 cut(s) 142, 532
CfoI GCGC 2 cut(s) 109, 117
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 380
Csp6I GTAC 1 cut(s) 847
CviAII CATG 2 cut(s) 145, 695
CviQI GTAC 1 cut(s) 847
DdeI CTNAG 4 cut(s) 161, 171, 445, 600
DpnI GATC 2 cut(s) 191, 781
DpnII GATC 2 cut(s) 189, 779
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 3 cut(s) 192, 219, 831
EarI CTCTTC 3 cut(s) 192, 219, 831
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 380
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 243, 339
Eco91I GGTNACC 1 cut(s) 615
EcoICRI GAGCTC 1 cut(s) 150
EcoO65I GGTNACC 1 cut(s) 615
EcoRI GAATTC 1 cut(s) 645
EcoT38I GRGCYC 1 cut(s) 152
FaeI CATG 2 cut(s) 148, 698
FalI AAGNNNNNCTT 2 cut(s) 683, 715
FatI CATG 2 cut(s) 144, 694
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 2 cut(s) 230, 300
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 2 cut(s) 230, 300
FspBI CTAG 3 cut(s) 128, 344, 453
GlaI GCGC 2 cut(s) 108, 116
GluI GCNGC 2 cut(s) 230, 300
GsuI CTGGAG 1 cut(s) 95
HaeII RGCGCY 2 cut(s) 110, 118
HaeIII GGCC 1 cut(s) 302
HapII CCGG 1 cut(s) 531
HhaI GCGC 2 cut(s) 109, 117
Hin1II CATG 2 cut(s) 148, 698
Hin6I GCGC 2 cut(s) 107, 115
HinP1I GCGC 2 cut(s) 107, 115
HinfI GANTC 7 cut(s) 34, 124, 175, 281, 351, 664, 889
HpaII CCGG 1 cut(s) 531
HphI GGTGA 3 cut(s) 383, 433, 609
Hpy166II GTNNAC 2 cut(s) 383, 493
Hpy188I TCNGA 4 cut(s) 33, 164, 174, 448
Hpy188III TCNNGA 2 cut(s) 179, 896
Hpy8I GTNNAC 2 cut(s) 383, 493
HpyAV CCTTC 3 cut(s) 7, 178, 226
HpyCH4III ACNGT 3 cut(s) 432, 527, 913
HpyCH4IV ACGT 2 cut(s) 254, 682
HpyCH4V TGCA 8 cut(s) 413, 483, 506, 566, 571, 767, 833, 841
HpyF10VI GCNNNNNNNGC 3 cut(s) 700, 709, 764
HpyF3I CTNAG 4 cut(s) 161, 171, 445, 600
HpySE526I ACGT 2 cut(s) 254, 682
Hsp92II CATG 2 cut(s) 148, 698
HspAI GCGC 2 cut(s) 107, 115
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Kzo9I GATC 2 cut(s) 189, 779
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 2 cut(s) 114, 708
LpnPI CCDG 9 cut(s) 125, 126, 164, 544, 576, 618, 759, 819, 833
LweI GCATC 3 cut(s) 37, 439, 574
MaeI CTAG 3 cut(s) 128, 344, 453
MaeII ACGT 2 cut(s) 254, 682
MaeIII GTNAC 3 cut(s) 339, 439, 615
MalI GATC 2 cut(s) 191, 781
MboI GATC 2 cut(s) 189, 779
MflI RGATCY 1 cut(s) 779
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 4 cut(s) 554, 610, 645, 658
MlyI GAGTC 1 cut(s) 658
MroNI GCCGGC 1 cut(s) 530
MseI TTAA 2 cut(s) 420, 465
MslI CAYNNNNRTG 2 cut(s) 237, 273
MspI CCGG 1 cut(s) 531
MwoI GCNNNNNNNGC 3 cut(s) 700, 709, 764
NaeI GCCGGC 1 cut(s) 532
NdeII GATC 2 cut(s) 189, 779
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 2 cut(s) 148, 698
NlaIV GGNNCC 2 cut(s) 792, 818
NmuCI GTSAC 2 cut(s) 439, 615
NspI RCATGY 1 cut(s) 698
PciSI GCTCTTC 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PdiI GCCGGC 1 cut(s) 532
PfeI GAWTC 6 cut(s) 34, 124, 175, 281, 351, 889
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 231, 301
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
Psp124BI GAGCTC 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 682
PspEI GGTNACC 1 cut(s) 615
PspN4I GGNNCC 2 cut(s) 792, 818
PspPI GGNCC 1 cut(s) 380
PsuI RGATCY 1 cut(s) 779
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 1 cut(s) 848
RsaNI GTAC 1 cut(s) 847
RseI CAYNNNNRTG 2 cut(s) 237, 273
SacI GAGCTC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 2 cut(s) 420, 465
SatI GCNGC 2 cut(s) 230, 300
Sau3AI GATC 2 cut(s) 189, 779
Sau96I GGNCC 1 cut(s) 380
SchI GAGTC 1 cut(s) 658
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 3 cut(s) 37, 439, 574
SfcI CTRYAG 1 cut(s) 523
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 2 cut(s) 237, 273
Sse9I AATT 4 cut(s) 554, 610, 645, 658
SsiI CCGC 3 cut(s) 230, 299, 668
SspMI CTAG 3 cut(s) 128, 344, 453
SstI GAGCTC 1 cut(s) 152
TaaI ACNGT 3 cut(s) 432, 527, 913
TaiI ACGT 2 cut(s) 257, 685
TaqI TCGA 3 cut(s) 152, 192, 753
TasI AATT 4 cut(s) 554, 610, 645, 658
TauI GCSGC 2 cut(s) 232, 302
TfiI GAWTC 6 cut(s) 34, 124, 175, 281, 351, 889
Tru1I TTAA 2 cut(s) 420, 465
Tru9I TTAA 2 cut(s) 420, 465
TscAI CASTG 3 cut(s) 226, 532, 604
TseFI GTSAC 2 cut(s) 439, 615
Tsp45I GTSAC 2 cut(s) 439, 615
TspDTI ATGAA 4 cut(s) 255, 294, 530, 648
TspRI CASTG 3 cut(s) 226, 532, 604
Tth111I GACNNNGTC 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 3 cut(s) 554, 645, 658
XceI RCATGY 1 cut(s) 698
XmiI GTMKAC 1 cut(s) 492
XspI CTAG 3 cut(s) 128, 344, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.