Rh6CG348600

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0018627
Physical Location & Seq
Reverse (-)
53920336 .. 53922503
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG348600.1

Sequence Viewer

Length: 660 bp
ATGGAGGCCATACTGAATGAGACTGAAGACGAAGATGAGGAGAAGTTACTAGAGCGATTCTTATCAAAGGAGGAGGATGATGATGGTCCACAGCGTAATCTCGCAGACCTCATACTTGCAACCATTAAGAACAAACAGTATGATGTCACCTATGCTAGGTCTTTGGTTAAAATGGATACAGATTGCATAGAAGTCTACAAGGGGGTTGCAAAGTTTATGAAAAGCTACAGTGCCGGCAAGATTGCCAAACCATTCAAATTTATCCCTGCACTGCAACAATGGGAGGATGCCCTCTATTTCACTGAGACTGATAATTGGTCACCAACTGCTATGTATGAAGCTACAAGAATTCTTGCTTCTAATTTGACTCCACAAAAAGCAGAACGTTTCTACAAGCATGTCTCGCTCCCACGGGTGAGACAAGAAATTCGGAATAAGAAGAAGAAGAAGCCCTTGCATTTTTCCCTGTATCAATCTCTAAGGAAGTCCGTCCACAAGCGTGCTGCCTTTTTTAAAGTAATACTGTTGCCTTTGTGTGAGTCAAGGACATGCACTCTAAAAGAAGCCTTCGTTGTTGGGAGCATCGATCATACAAAAGGTTCGAATTTCTGCAAGTCATGCAGGTGCTGCAATAATGATGCTAGCAGAGATGGAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

219

Amino Acids

25.29

Weight (kDa)

8.85

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 30 - 195 1.6e-54 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018627)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0291881
rosa_multiflora Rmu_sc0002484.1_g000003
rosa_roxburghii Rroxscaffold_7G00175660
rosa_rugosa Rorug06G0221700 Rorug06G0221700
rosa_samantha Rh6AG335100 Rh6BG342500 Rh6CG348600 Rh6DG335300
rosa_wichuraiana Rw6G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 612
Acc36I ACCTGC 1 cut(s) 612
AccI GTMKAC 1 cut(s) 195
AclI AACGTT 1 cut(s) 385
AcsI RAATTY 4 cut(s) 257, 348, 426, 604
AcuI CTGAAG 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 156
AgsI TTSAA 1 cut(s) 256
AleI CACNNNNGTG 1 cut(s) 498
AluBI AGCT 2 cut(s) 225, 341
AluI AGCT 2 cut(s) 225, 341
Alw26I GTCTC 4 cut(s) 14, 299, 406, 412
AlwNI CAGNNNCTG 1 cut(s) 627
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 503, 627
ApoI RAATTY 4 cut(s) 257, 348, 426, 604
ArsI GACNNNNNNTTYG 2 cut(s) 411, 443
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 3 cut(s) 139, 312, 427
AsuII TTCGAA 1 cut(s) 602
AsuNHI GCTAGC 1 cut(s) 641
AvaII GGWCC 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 490, 614
BccI CCATC 2 cut(s) 77, 644
BciVI GTATCC 1 cut(s) 169
BcoDI GTCTC 4 cut(s) 14, 299, 406, 412
BfaI CTAG 3 cut(s) 50, 156, 642
BfmI CTRYAG 1 cut(s) 226
BfuAI ACCTGC 1 cut(s) 612
BfuI GTATCC 1 cut(s) 169
BisI GCNGC 2 cut(s) 504, 628
BlsI GCNGC 2 cut(s) 505, 629
Bme18I GGWCC 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 86
BmsI GCATC 3 cut(s) 277, 591, 628
BmtI GCTAGC 1 cut(s) 645
BpiI GAAGAC 1 cut(s) 33
Bpu14I TTCGAA 1 cut(s) 602
Bsa29I ATCGAT 1 cut(s) 585
BsaBI GATNNNNATC 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 410
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse118I RCCGGY 1 cut(s) 233
Bse8I GATNNNNATC 1 cut(s) 61
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 1 cut(s) 410
BseGI GGATG 2 cut(s) 82, 292
BseJI GATNNNNATC 1 cut(s) 61
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMII CTCAG 1 cut(s) 294
BseRI GAGGAG 2 cut(s) 53, 86
BseXI GCAGC 2 cut(s) 490, 614
BsgI GTGCAG 1 cut(s) 252
BshFI GGCC 1 cut(s) 8
BshVI ATCGAT 1 cut(s) 585
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 4 cut(s) 14, 299, 406, 412
BsnI GGCC 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 602
Bsp143I GATC 1 cut(s) 586
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 295
BspDI ATCGAT 1 cut(s) 585
BspMI ACCTGC 1 cut(s) 612
BspOI GCTAGC 1 cut(s) 645
BspT104I TTCGAA 1 cut(s) 602
BsrFI RCCGGY 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 1 cut(s) 410
BssMI GATC 1 cut(s) 586
Bst4CI ACNGT 3 cut(s) 138, 230, 525
BstAPI GCANNNNNTGC 2 cut(s) 618, 627
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 3 cut(s) 235, 501, 643
BstDEI CTNAG 2 cut(s) 303, 479
BstDSI CCRYGG 1 cut(s) 410
BstEII GGTNACC 1 cut(s) 318
BstENI CCTNNNNNAGG 1 cut(s) 154
BstF5I GGATG 2 cut(s) 82, 292
BstKTI GATC 1 cut(s) 589
BstMAI GTCTC 4 cut(s) 14, 299, 406, 412
BstMBI GATC 1 cut(s) 586
BstMWI GCNNNNNNNGC 3 cut(s) 403, 618, 627
BstNSI RCATGY 2 cut(s) 401, 552
BstPI GGTNACC 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 226
BstV1I GCAGC 2 cut(s) 490, 614
BstV2I GAAGAC 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 585
BsuI GTATCC 1 cut(s) 169
BsuRI GGCC 1 cut(s) 8
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 1 cut(s) 410
BtsCI GGATG 2 cut(s) 82, 292
BtsI GCAGTG 1 cut(s) 269
BtsIMutI CAGTG 3 cut(s) 235, 269, 300
BveI ACCTGC 1 cut(s) 612
Cac8I GCNNGC 3 cut(s) 235, 501, 643
CaiI CAGNNNCTG 1 cut(s) 627
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 86
ClaI ATCGAT 1 cut(s) 585
CviAII CATG 3 cut(s) 398, 549, 618
CviJI RGCY 5 cut(s) 8, 225, 341, 451, 566
CviKI_1 RGCY 5 cut(s) 8, 225, 341, 451, 566
DdeI CTNAG 2 cut(s) 303, 479
DpnI GATC 1 cut(s) 588
DpnII GATC 1 cut(s) 586
DraI TTTAAA 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 45
Eco91I GGTNACC 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 154
EcoO65I GGTNACC 1 cut(s) 318
EcoRI GAATTC 1 cut(s) 348
FaeI CATG 3 cut(s) 401, 552, 621
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FatI CATG 3 cut(s) 397, 548, 617
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 2 cut(s) 504, 628
FokI GGATG 2 cut(s) 89, 299
Fsp4HI GCNGC 2 cut(s) 504, 628
FspBI CTAG 3 cut(s) 50, 156, 642
GluI GCNGC 2 cut(s) 504, 628
HaeIII GGCC 1 cut(s) 8
HapII CCGG 1 cut(s) 234
Hin1II CATG 3 cut(s) 401, 552, 621
HinfI GANTC 3 cut(s) 57, 367, 539
HpaII CCGG 1 cut(s) 234
HphI GGTGA 3 cut(s) 139, 312, 427
Hpy166II GTNNAC 3 cut(s) 89, 196, 493
Hpy188I TCNGA 1 cut(s) 432
Hpy8I GTNNAC 3 cut(s) 89, 196, 493
HpyAV CCTTC 1 cut(s) 577
HpyCH4III ACNGT 3 cut(s) 138, 230, 525
HpyCH4IV ACGT 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 3 cut(s) 403, 618, 627
HpyF3I CTNAG 2 cut(s) 303, 479
HpySE526I ACGT 1 cut(s) 385
Hsp92II CATG 3 cut(s) 401, 552, 621
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
Kzo9I GATC 1 cut(s) 586
LmnI GCTCC 2 cut(s) 411, 579
LpnPI CCDG 4 cut(s) 247, 279, 479, 607
Lsp1109I GCAGC 2 cut(s) 490, 614
LweI GCATC 3 cut(s) 277, 591, 628
MaeI CTAG 3 cut(s) 50, 156, 642
MaeII ACGT 1 cut(s) 385
MaeIII GTNAC 3 cut(s) 45, 145, 318
MalI GATC 1 cut(s) 588
MboI GATC 1 cut(s) 586
MboII GAAGA 5 cut(s) 38, 44, 451, 454, 457
MluCI AATT 6 cut(s) 257, 313, 348, 361, 426, 604
MlyI GAGTC 2 cut(s) 361, 548
MnlI CCTC 6 cut(s) 31, 64, 67, 119, 277, 302
MroNI GCCGGC 1 cut(s) 233
MseI TTAA 3 cut(s) 126, 168, 513
MslI CAYNNNNRTG 2 cut(s) 498, 622
MspI CCGG 1 cut(s) 234
MwoI GCNNNNNNNGC 3 cut(s) 403, 618, 627
NaeI GCCGGC 1 cut(s) 235
NdeII GATC 1 cut(s) 586
NgoMIV GCCGGC 1 cut(s) 233
NheI GCTAGC 1 cut(s) 641
NlaIII CATG 3 cut(s) 401, 552, 621
NmuCI GTSAC 2 cut(s) 145, 318
NspI RCATGY 2 cut(s) 401, 552
NspV TTCGAA 1 cut(s) 602
OliI CACNNNNGTG 1 cut(s) 498
PaqCI CACCTGC 1 cut(s) 612
PdiI GCCGGC 1 cut(s) 235
PfeI GAWTC 1 cut(s) 57
PkrI GCNGC 2 cut(s) 505, 629
PleI GAGTC 2 cut(s) 361, 547
PpsI GAGTC 2 cut(s) 361, 547
Psp1406I AACGTT 1 cut(s) 385
PspEI GGTNACC 1 cut(s) 318
PspPI GGNCC 1 cut(s) 86
PstNI CAGNNNCTG 1 cut(s) 627
RseI CAYNNNNRTG 2 cut(s) 498, 622
SaqAI TTAA 3 cut(s) 126, 168, 513
SatI GCNGC 2 cut(s) 504, 628
Sau3AI GATC 1 cut(s) 586
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 2 cut(s) 361, 548
SetI ASST 8 cut(s) 111, 152, 161, 227, 343, 388, 601, 626
SfaNI GCATC 3 cut(s) 277, 591, 628
SfcI CTRYAG 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 498, 622
Sse9I AATT 6 cut(s) 257, 313, 348, 361, 426, 604
SspMI CTAG 3 cut(s) 50, 156, 642
TaaI ACNGT 3 cut(s) 138, 230, 525
TaiI ACGT 1 cut(s) 388
TaqI TCGA 2 cut(s) 585, 602
TasI AATT 6 cut(s) 257, 313, 348, 361, 426, 604
TfiI GAWTC 1 cut(s) 57
Tru1I TTAA 3 cut(s) 126, 168, 513
Tru9I TTAA 3 cut(s) 126, 168, 513
TscAI CASTG 3 cut(s) 235, 276, 307
TseFI GTSAC 2 cut(s) 145, 318
TseI GCWGC 2 cut(s) 503, 627
Tsp45I GTSAC 2 cut(s) 145, 318
TspDTI ATGAA 2 cut(s) 233, 351
TspGWI ACGGA 1 cut(s) 478
TspRI CASTG 3 cut(s) 235, 276, 307
VpaK11BI GGWCC 1 cut(s) 86
XagI CCTNNNNNAGG 1 cut(s) 154
XapI RAATTY 4 cut(s) 257, 348, 426, 604
XceI RCATGY 2 cut(s) 401, 552
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 3 cut(s) 50, 156, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.