Rh6DG335300

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0018627
Physical Location & Seq
Reverse (-)
54015191 .. 54019167
3977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG335300.1

Sequence Viewer

Length: 1272 bp
ATGACGAAGAAGAGGGGGAGAGAGAGGCATCGGAATCCAAAGCCGTTCCTCGCCGAAGACGATGTCTCTGTGGCTTCCACAAAGCGGCTCAAAATGGCGCGCAAGCGCCAAGAGAATCTAGTTTTATCAGCAGGCATGAGCTCGAAAATCCTCAGGGAAGCTCAGATTCAGGAGAAGGAGATCGAGGAAGAGAAGAACACAAACGCCTTCACTGAAGAGCCACACAACGATGAAGAGGACGACGATGACTTCGATACGTTCCTTGAAACACATAGAGAGGATGAGGCAAACAAAGTGATGGAGGCCATAATGAATGAGACTGAAGACGAAGATGAGGAGAAGTTACTAGAGCGATTCTTATCAAAGGAGGAGGGTGATGATGGTCCACAGCGTAATCTCGCAGACCTCATACTTGCAACCATTAAGAACAAACAGTATGATGTCACCTCAGATGCTAGGTCTTTGGTTAAAATGGATACAGATTTCATAGAAGTCTACAAGGGGGTTGCAAAGTTTATGAAAAGCTACAGTGCCGGCAAGATTGCCAAACCATTCAAATTTATCCCTGCAATGCAACAATGGGAGGATGCCCTCTATTTCACTGAGCCTGATAATTGGTCACCAACTGCTATGTATGAAGCTACAAGAATTCTTGCTTCTAATTTGACTCCGCAAAAAGCAGAACGTTTCTACAAGCATGTCATGCTCCCACGGGTGAGACAAGACATTCGGAATAAGAAGAAGAAGAAGAAGCCCTTGCATTTTTCCCTATATCAATCTCTAAGGAAGTCCGTCCACAAGCGTGCTGCCTTTTTTAAAGGAATACTGTTGCCTTTGTGTGAGTCAAGGACGTGCACTCTAAAAGAAGCCTCCGTTGTTGGGAGCATCATACAAAAGGTTCGAATTTCTGCAAGTCATGCAGGTGCTGCAATAATGATGCTAGCAGAGATGGAATATAGCAGCGCAAGAAGTTATTTTATAAAGCTTCTTTTGGAGAAACAGTATGCTTTGCCAAATCCTGTAATCGAGGCTCTTGTTGCACATTTTATGAGATCTGTGGAGGAACCAAGAGTAATGCCTGTGATATGGCACCAGTCTCTTCTTGCATTTGTGCAAAGGTACAAAAATGATATAACAAAGGACGACAAAGATAAGCTTAGGAATCTTCTTGAAGCACAAAACCACCTTGAGGTGGGTCTGGCTAAAGAAATAATGCACAAGTACATGCTTGGGAGGAAATGCAATACTGTTCTTGCATTGGAATGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

423

Amino Acids

49.12

Weight (kDa)

8.43

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 129 - 408 1.8e-104 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018627)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0291881
rosa_multiflora Rmu_sc0002484.1_g000003
rosa_roxburghii Rroxscaffold_7G00175660
rosa_rugosa Rorug06G0221700 Rorug06G0221700
rosa_samantha Rh6AG335100 Rh6BG342500 Rh6CG348600 Rh6DG335300
rosa_wichuraiana Rw6G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 980
AarI CACCTGC 1 cut(s) 911
Acc36I ACCTGC 1 cut(s) 911
AccB1I GGYRCC 1 cut(s) 1089
AccI GTMKAC 1 cut(s) 495
AccII CGCG 1 cut(s) 100
AciI CCGC 2 cut(s) 85, 671
AclI AACGTT 1 cut(s) 685
AcsI RAATTY 3 cut(s) 557, 648, 903
AcuI CTGAAG 2 cut(s) 234, 342
AfaI GTAC 2 cut(s) 1121, 1223
AfiI CCNNNNNNNGG 4 cut(s) 84, 879, 1189, 1192
AgsI TTSAA 3 cut(s) 266, 556, 1172
AjiI CACGTC 1 cut(s) 852
AleI CACNNNNGTG 1 cut(s) 801
AluBI AGCT 6 cut(s) 141, 161, 525, 641, 985, 1156
AluI AGCT 6 cut(s) 141, 161, 525, 641, 985, 1156
Alw21I GWGCWC 2 cut(s) 143, 857
Alw26I GTCTC 4 cut(s) 70, 311, 712, 1101
Alw44I GTGCAC 1 cut(s) 853
AlwNI CAGNNNCTG 1 cut(s) 926
AoxI GGCC 1 cut(s) 303
ApaLI GTGCAC 1 cut(s) 853
ApeKI GCWGC 3 cut(s) 806, 926, 960
ApoI RAATTY 3 cut(s) 557, 648, 903
ArsI GACNNNNNNTTYG 6 cut(s) 48, 80, 233, 265, 711, 743
AspLEI GCGC 4 cut(s) 100, 102, 108, 965
AspS9I GGNCC 1 cut(s) 383
AsuHPI GGTGA 4 cut(s) 386, 436, 612, 727
AsuII TTCGAA 1 cut(s) 901
AsuNHI GCTAGC 1 cut(s) 940
AvaII GGWCC 1 cut(s) 383
AxyI CCTNAGG 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 857
BanI GGYRCC 1 cut(s) 1089
BanII GRGCYC 1 cut(s) 143
BbsI GAAGAC 2 cut(s) 63, 330
Bbv12I GWGCWC 2 cut(s) 143, 857
BbvI GCAGC 3 cut(s) 793, 913, 972
BccI CCATC 3 cut(s) 292, 374, 943
BceAI ACGGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 469
BcoDI GTCTC 4 cut(s) 70, 311, 712, 1101
BfaI CTAG 4 cut(s) 119, 347, 456, 941
BfmI CTRYAG 1 cut(s) 526
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 911
BfuI GTATCC 1 cut(s) 469
BglII AGATCT 1 cut(s) 1052
BisI GCNGC 4 cut(s) 86, 807, 927, 961
BlsI GCNGC 4 cut(s) 87, 808, 928, 962
Bme18I GGWCC 1 cut(s) 383
BmgBI CACGTC 1 cut(s) 852
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 2 cut(s) 1065, 1091
BmsI GCATC 5 cut(s) 37, 442, 577, 894, 927
BmtI GCTAGC 1 cut(s) 944
BpiI GAAGAC 2 cut(s) 63, 330
Bpu10I CCTNAGC 1 cut(s) 1157
Bpu14I TTCGAA 1 cut(s) 901
BpuEI CTTGAG 1 cut(s) 1208
BsaBI GATNNNNATC 1 cut(s) 358
BsaJI CCNNGG 1 cut(s) 710
BsaXI ACNNNNNCTCC 2 cut(s) 986, 1016
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 879, 1189, 1192
Bse118I RCCGGY 1 cut(s) 533
Bse1I ACTGG 1 cut(s) 1093
Bse21I CCTNAGG 1 cut(s) 152
Bse3DI GCAATG 1 cut(s) 576
Bse8I GATNNNNATC 1 cut(s) 358
BseDI CCNNGG 1 cut(s) 710
BseGI GGATG 2 cut(s) 286, 592
BseJI GATNNNNATC 1 cut(s) 358
BseLI CCNNNNNNNGG 4 cut(s) 84, 879, 1189, 1192
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 4 cut(s) 166, 176, 462, 594
BseNI ACTGG 1 cut(s) 1093
BsePI GCGCGC 1 cut(s) 98
BseRI GAGGAG 2 cut(s) 350, 383
BseSI GKGCMC 1 cut(s) 857
BseXI GCAGC 3 cut(s) 793, 913, 972
Bsh1236I CGCG 1 cut(s) 100
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 1089
BsiHKAI GWGCWC 2 cut(s) 143, 857
BsiSI CCGG 1 cut(s) 534
BslI CCNNNNNNNGG 4 cut(s) 84, 879, 1189, 1192
BsmAI GTCTC 4 cut(s) 70, 311, 712, 1101
BsnI GGCC 1 cut(s) 305
Bsp119I TTCGAA 1 cut(s) 901
Bsp1286I GDGCHC 2 cut(s) 143, 857
Bsp143I GATC 2 cut(s) 180, 1052
BspACI CCGC 2 cut(s) 85, 671
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 4 cut(s) 165, 175, 461, 595
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 2 cut(s) 1065, 1091
BspMI ACCTGC 1 cut(s) 911
BspOI GCTAGC 1 cut(s) 944
BspQI GCTCTTC 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 901
BspT107I GGYRCC 1 cut(s) 1089
BsrDI GCAATG 1 cut(s) 576
BsrFI RCCGGY 1 cut(s) 533
BsrI ACTGG 1 cut(s) 1093
BssAI RCCGGY 1 cut(s) 533
BssECI CCNNGG 1 cut(s) 710
BssHII GCGCGC 1 cut(s) 98
BssMI GATC 2 cut(s) 180, 1052
Bst4CI ACNGT 5 cut(s) 435, 530, 828, 1002, 1249
Bst6I CTCTTC 5 cut(s) 5, 183, 210, 228, 1104
BstAPI GCANNNNNTGC 3 cut(s) 703, 917, 926
BstBI TTCGAA 1 cut(s) 901
BstC8I GCNNGC 6 cut(s) 100, 104, 133, 535, 804, 942
BstDEI CTNAG 6 cut(s) 152, 162, 448, 603, 782, 1157
BstDSI CCRYGG 1 cut(s) 710
BstEII GGTNACC 1 cut(s) 618
BstF5I GGATG 2 cut(s) 286, 592
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 4 cut(s) 100, 102, 108, 965
BstKTI GATC 2 cut(s) 183, 1055
BstMAI GTCTC 4 cut(s) 70, 311, 712, 1101
BstMBI GATC 2 cut(s) 180, 1052
BstMWI GCNNNNNNNGC 4 cut(s) 703, 917, 926, 1037
BstNSI RCATGY 2 cut(s) 701, 1228
BstPI GGTNACC 1 cut(s) 618
BstSFI CTRYAG 1 cut(s) 526
BstSLI GKGCMC 1 cut(s) 857
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 3 cut(s) 793, 913, 972
BstV2I GAAGAC 2 cut(s) 63, 330
BstX2I RGATCY 1 cut(s) 1052
BstYI RGATCY 1 cut(s) 1052
Bsu36I CCTNAGG 1 cut(s) 152
BsuI GTATCC 1 cut(s) 469
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 710
BtrI CACGTC 1 cut(s) 852
BtsCI GGATG 2 cut(s) 286, 592
BtsIMutI CAGTG 3 cut(s) 210, 535, 600
BveI ACCTGC 1 cut(s) 911
Cac8I GCNNGC 6 cut(s) 100, 104, 133, 535, 804, 942
CaiI CAGNNNCTG 1 cut(s) 926
CfoI GCGC 4 cut(s) 100, 102, 108, 965
Cfr10I RCCGGY 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 383
Csp6I GTAC 2 cut(s) 1120, 1222
CviAII CATG 5 cut(s) 136, 698, 703, 917, 1225
CviQI GTAC 2 cut(s) 1120, 1222
DdeI CTNAG 6 cut(s) 152, 162, 448, 603, 782, 1157
DpnI GATC 2 cut(s) 182, 1054
DpnII GATC 2 cut(s) 180, 1052
DraI TTTAAA 1 cut(s) 817
Eam1104I CTCTTC 5 cut(s) 5, 183, 210, 228, 1104
EarI CTCTTC 5 cut(s) 5, 183, 210, 228, 1104
Ecl136II GAGCTC 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 383
Eco53kI GAGCTC 1 cut(s) 141
Eco57I CTGAAG 2 cut(s) 234, 342
Eco81I CCTNAGG 1 cut(s) 152
Eco91I GGTNACC 1 cut(s) 618
EcoICRI GAGCTC 1 cut(s) 141
EcoO65I GGTNACC 1 cut(s) 618
EcoRI GAATTC 1 cut(s) 648
EcoT38I GRGCYC 1 cut(s) 143
FaeI CATG 5 cut(s) 139, 701, 706, 920, 1228
FalI AAGNNNNNCTT 4 cut(s) 740, 772, 1140, 1172
FatI CATG 5 cut(s) 135, 697, 702, 916, 1224
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 4 cut(s) 86, 807, 927, 961
FokI GGATG 2 cut(s) 293, 599
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 4 cut(s) 86, 807, 927, 961
FspBI CTAG 4 cut(s) 119, 347, 456, 941
GlaI GCGC 4 cut(s) 99, 101, 107, 964
GluI GCNGC 4 cut(s) 86, 807, 927, 961
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 534
HhaI GCGC 4 cut(s) 100, 102, 108, 965
Hin1II CATG 5 cut(s) 139, 701, 706, 920, 1228
Hin6I GCGC 4 cut(s) 98, 100, 106, 963
HinP1I GCGC 4 cut(s) 98, 100, 106, 963
HindIII AAGCTT 2 cut(s) 983, 1154
HinfI GANTC 7 cut(s) 34, 115, 166, 354, 667, 842, 1162
HpaII CCGG 1 cut(s) 534
HphI GGTGA 4 cut(s) 386, 436, 612, 727
Hpy166II GTNNAC 4 cut(s) 386, 496, 796, 855
Hpy188I TCNGA 4 cut(s) 33, 165, 451, 732
Hpy188III TCNNGA 2 cut(s) 170, 1169
Hpy8I GTNNAC 4 cut(s) 386, 496, 796, 855
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 2 cut(s) 169, 217
HpyCH4III ACNGT 5 cut(s) 435, 530, 828, 1002, 1249
HpyCH4IV ACGT 3 cut(s) 257, 685, 851
HpyF10VI GCNNNNNNNGC 4 cut(s) 703, 917, 926, 1037
HpyF3I CTNAG 6 cut(s) 152, 162, 448, 603, 782, 1157
HpySE526I ACGT 3 cut(s) 257, 685, 851
Hsp92II CATG 5 cut(s) 139, 701, 706, 920, 1228
HspAI GCGC 4 cut(s) 98, 100, 106, 963
KroI GCCGGC 1 cut(s) 533
KroNI GCCGGC 1 cut(s) 535
Kzo9I GATC 2 cut(s) 180, 1052
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 2 cut(s) 711, 882
Lsp1109I GCAGC 3 cut(s) 793, 913, 972
LweI GCATC 5 cut(s) 37, 442, 577, 894, 927
MaeI CTAG 4 cut(s) 119, 347, 456, 941
MaeII ACGT 3 cut(s) 257, 685, 851
MaeIII GTNAC 3 cut(s) 342, 442, 618
MalI GATC 2 cut(s) 182, 1054
MboI GATC 2 cut(s) 180, 1052
MflI RGATCY 1 cut(s) 1052
MhlI GDGCHC 2 cut(s) 143, 857
MluCI AATT 5 cut(s) 557, 613, 648, 661, 903
MlyI GAGTC 2 cut(s) 661, 851
MroNI GCCGGC 1 cut(s) 533
MseI TTAA 4 cut(s) 423, 468, 816, 1270
MslI CAYNNNNRTG 4 cut(s) 228, 801, 921, 1261
MspI CCGG 1 cut(s) 534
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 4 cut(s) 703, 917, 926, 1037
NaeI GCCGGC 1 cut(s) 535
NdeII GATC 2 cut(s) 180, 1052
NgoMIV GCCGGC 1 cut(s) 533
NheI GCTAGC 1 cut(s) 940
NlaIII CATG 5 cut(s) 139, 701, 706, 920, 1228
NlaIV GGNNCC 2 cut(s) 1065, 1091
NmuCI GTSAC 2 cut(s) 442, 618
NspI RCATGY 2 cut(s) 701, 1228
NspV TTCGAA 1 cut(s) 901
OliI CACNNNNGTG 1 cut(s) 801
PaqCI CACCTGC 1 cut(s) 911
PauI GCGCGC 1 cut(s) 98
PciSI GCTCTTC 1 cut(s) 210
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 249
PdiI GCCGGC 1 cut(s) 535
PfeI GAWTC 5 cut(s) 34, 115, 166, 354, 1162
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 4 cut(s) 87, 808, 928, 962
PleI GAGTC 2 cut(s) 661, 850
PpsI GAGTC 2 cut(s) 661, 850
PsiI TTATAA 1 cut(s) 980
Psp124BI GAGCTC 1 cut(s) 143
Psp1406I AACGTT 1 cut(s) 685
PspEI GGTNACC 1 cut(s) 618
PspN4I GGNNCC 2 cut(s) 1065, 1091
PspPI GGNCC 1 cut(s) 383
PstNI CAGNNNCTG 1 cut(s) 926
PsuI RGATCY 1 cut(s) 1052
PsyI GACNNNGTC 1 cut(s) 62
PteI GCGCGC 1 cut(s) 98
RsaI GTAC 2 cut(s) 1121, 1223
RsaNI GTAC 2 cut(s) 1120, 1222
RseI CAYNNNNRTG 4 cut(s) 228, 801, 921, 1261
SacI GAGCTC 1 cut(s) 143
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 4 cut(s) 423, 468, 816, 1270
SatI GCNGC 4 cut(s) 86, 807, 927, 961
Sau3AI GATC 2 cut(s) 180, 1052
Sau96I GGNCC 1 cut(s) 383
SchI GAGTC 2 cut(s) 661, 851
SduI GDGCHC 2 cut(s) 143, 857
SfaNI GCATC 5 cut(s) 37, 442, 577, 894, 927
SfcI CTRYAG 1 cut(s) 526
SfuI TTCGAA 1 cut(s) 901
SinI GGWCC 1 cut(s) 383
SmiMI CAYNNNNRTG 4 cut(s) 228, 801, 921, 1261
SmlI CTYRAG 1 cut(s) 1187
SmoI CTYRAG 1 cut(s) 1187
Sse9I AATT 5 cut(s) 557, 613, 648, 661, 903
SsiI CCGC 2 cut(s) 85, 671
SspMI CTAG 4 cut(s) 119, 347, 456, 941
SstI GAGCTC 1 cut(s) 143
TaaI ACNGT 5 cut(s) 435, 530, 828, 1002, 1249
TaiI ACGT 3 cut(s) 260, 688, 854
TaqI TCGA 5 cut(s) 143, 183, 252, 901, 1026
TasI AATT 5 cut(s) 557, 613, 648, 661, 903
TatI WGTACW 1 cut(s) 1221
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 5 cut(s) 34, 115, 166, 354, 1162
Tru1I TTAA 4 cut(s) 423, 468, 816, 1270
Tru9I TTAA 4 cut(s) 423, 468, 816, 1270
TscAI CASTG 3 cut(s) 217, 535, 607
TseFI GTSAC 2 cut(s) 442, 618
TseI GCWGC 3 cut(s) 806, 926, 960
Tsp45I GTSAC 2 cut(s) 442, 618
TspDTI ATGAA 5 cut(s) 246, 326, 475, 533, 651
TspGWI ACGGA 2 cut(s) 781, 862
TspRI CASTG 3 cut(s) 217, 535, 607
Tth111I GACNNNGTC 1 cut(s) 62
VneI GTGCAC 1 cut(s) 853
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 3 cut(s) 557, 648, 903
XceI RCATGY 2 cut(s) 701, 1228
XmiI GTMKAC 1 cut(s) 495
XspI CTAG 4 cut(s) 119, 347, 456, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.