RchiOBHm_Chr6g0298101

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0016269
Physical Location & Seq
Forward (+)
59633570 .. 59636307
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26758

Sequence Viewer

Length: 1998 bp
ATGGTTTTTCTTCTCTTCTTCTCTACCTTTCTTCTTATTCACAACAACCAAGCCTCTTTGAACGATCCTATCAGTTTCTCCTTCCCCACCTTCACTCCTCAAAGTTGCAGCAGTGAAGGTAAATTACTATGCATGGGATCAGTGACTTCAGGTGAAGGGTATTTGAGTCTCACACCCGAGCAACAAAAGAGCAACTCAAGTTCAGCATCGGTTTTACCGCTATACAAAGTTGGGAGGGTTCTGTATCGGTACCCAGTTCCGGCATGGTCTTCTTTTATCTCCACAACCTTCACTGTCAGGATTTCGGCTTTCTCAAATACAACTGGCTCTGGAGATGGAATGGCATTTGTTTTTGCTCAGGATAGTCGTCCTTCTCCTCCTGATAGCTATGGCTCATTTCTTGGACTCCTAGATAGATCAACACAAGGTGGGGTGATTAAGCAGTTGGGAATTGAGCTAGACACTTTCATGAACCATGAGTTTGATCCAGATGACAACCACATTGCCATTGACACAACAAGCATTATGGACCCAGTTGCAATACAGAGTCTCAACAGTTCAGGCATTAACCTCAAGAGTGGGAGAGACATCAAGTTCACAATTGAGTATAATGGTTGGAACCAAATCCTCCACGTTTCAGCTGGCTACTCTGAGAACCCACTAGTCACCATCTTAAACCATTCAATCTCCATGTTCGACAGTATTGTTCCGAGCTCCGTTTATGTTGGCTTCACAGCATCTACTGGAACTCTCCCTGAGAGCCACCAAGTCCGTGATTGGGTTTTCACCTCCCTTCAGTTGCCAAATCCTAGCCCTCCTGGGAATGACAAAGGGCGTAAGAGAAAGCCCAGGAACATTTGGACTGTTGATGTGCCTGTTTTTCTGTGTGTGTTGGTTTTGATGGCCTTCACTTACCCTTTCATTTTGAGAGTTGTGAAAAGAAACCACTGTGGTGGGAATGAGACAGAAGACATAGAAAGCAGGACAAGAACAGCTGCAAATGCCCCTAAAATGTTTACTTACAAGGAGATTTCAAGGGCCACTCAGGACTTCAGCAAGGAAAATCTGATTGGGGTTGGTGGATTTGGAGTTGTCTACAAAGGCATCATACCAGATAAGATTCATGTTCCTCCTAAAACTATTGCTGTCAAGAAGATTACAGCAGCCTCCAAACAAGGGGAAAGGGAGTACCTAGCAGAAATATGCACAATTGGGCGCCTACGGCACAAGAACATAGTACAACTCCAAGGCTGGTGTCACGAAAACGAAAATCGTTTTTTAATCTATGAATACATGCCTAACGGGAGCCTCGACCGTTACATCGGAAATGGCATCCTTGATTGGAAAACAAGGTACAATATCTTAACAGGCTTGGCATCAGCATTGCTTTACCTCCATGAAGAATGTAGCAACCCTGTTGTTCACAGAGACATCAAGCCCAACAATGTGATGCTGGACTCTGACTACAATGCTCATCTAGGTGACTTTGGGTTAGCAAGAGTAATGCTACAAGATCCCAAGCTTACAATTCCCTTAGCCGGTACTCCCGGGTACTTGGCCCCGGAAGTATTAGGGTTTGCGGGAAAAGCTACCCCAGAATCGGATGTCTATAGCTTTGGAATCGTGGTGCTAGAAGTGGTGTGTGGGAGAAGATCAAAGGGTATCTTCGACGACTACAGTTTAGTAGAAAATGTATGGAATTTGTACGCGAAAGACGAGCTGCTCGAGTGCGTGGACCAAGTAGTGCTTGATGGGAAATTTGAAGTGGAACAAGTGAAGAGGACATTGATTGTTGGCCTTGCTTGCTTGCACACAGATTTTATGCTCAGGCCAAAGATAAGGAAGGTGGTGCAGATTTTGATGAACCCAAATGAGCCTCTACTAGATTTGCCAGATACAAGGCCAAGGCCTAGTGCAGTATATCTATCAGTATCTTCTTCTGCTCCTAGAATCACTGATTTTGGCTCTGTTGCCTCATCCAGTATCATGATGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase

Protein Analysis

665

Amino Acids

73.7

Weight (kDa)

6.3

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 25 - 267 2.9e-40 Legume lectin domain
Lectin_L-type_dom PF18483 77 - 258 5.1e-15 Legume lectin beta-barrel domain
Pkinase PF00069 352 - 551 1.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 354 - 574 3.3e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016269)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29290
malus_domestica MD08G1148700.v1.1 MD08G1148800.v1.1 MD08G1212600.v1.1 MD15G1124000.v1.1
prunus_persica Prupe.1G479400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298101
rosa_laevigata RLG00000011534
rosa_multiflora Rmu_sc0002800.1_g000004
rosa_rugosa Rorug06G0278900
rosa_samantha Rh6AG389000 Rh6BG397300 Rh6CG403200 Rh6DG389800
rosa_wichuraiana Rw6G033970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 249
AccB1I GGYRCC 2 cut(s) 249, 1215
AccI GTMKAC 1 cut(s) 1095
AccII CGCG 1 cut(s) 1709
AciI CCGC 2 cut(s) 218, 1580
AclWI GGATC 4 cut(s) 59, 145, 479, 1508
AcsI RAATTY 2 cut(s) 1699, 1757
AcuI CTGAAG 3 cut(s) 132, 779, 1036
AcyI GRCGYC 1 cut(s) 1216
AdeI CACNNNGTG 1 cut(s) 428
AfaI GTAC 7 cut(s) 251, 1190, 1239, 1355, 1543, 1553, 1706
AfiI CCNNNNNNNGG 5 cut(s) 259, 778, 1176, 1538, 1600
AgsI TTSAA 4 cut(s) 61, 684, 1035, 1763
AhlI ACTAGT 1 cut(s) 661
AjnI CCWGG 2 cut(s) 817, 848
AjuI GAANNNNNNNTTGG 2 cut(s) 1053, 1085
AleI CACNNNNGTG 1 cut(s) 951
AluBI AGCT 9 cut(s) 387, 457, 641, 714, 995, 1522, 1589, 1614, 1720
AluI AGCT 9 cut(s) 387, 457, 641, 714, 995, 1522, 1589, 1614, 1720
Alw21I GWGCWC 1 cut(s) 716
Alw26I GTCTC 5 cut(s) 173, 554, 579, 956, 1422
AlwI GGATC 4 cut(s) 59, 145, 479, 1508
Ama87I CYCGRG 3 cut(s) 176, 1547, 1724
AoxI GGCC 7 cut(s) 903, 1038, 1557, 1795, 1829, 1901, 1907
ApeKI GCWGC 4 cut(s) 108, 995, 1163, 1720
ApoI RAATTY 2 cut(s) 1699, 1757
Asp718I GGTACC 1 cut(s) 249
AspLEI GCGC 1 cut(s) 1218
AspS9I GGNCC 4 cut(s) 529, 1038, 1558, 1735
AsuC2I CCSGG 3 cut(s) 1548, 1549, 1562
AsuHPI GGTGA 5 cut(s) 164, 445, 658, 778, 1493
AvaI CYCGRG 3 cut(s) 176, 1547, 1724
AvaII GGWCC 2 cut(s) 529, 1735
BanI GGYRCC 2 cut(s) 249, 1215
BanII GRGCYC 1 cut(s) 716
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
BbsI GAAGAC 2 cut(s) 261, 975
Bbv12I GWGCWC 1 cut(s) 716
BbvI GCAGC 4 cut(s) 120, 982, 1175, 1707
BccI CCATC 4 cut(s) 329, 677, 895, 1745
BceAI ACGGC 1 cut(s) 1238
BciT130I CCWGG 2 cut(s) 819, 850
BcnI CCSGG 3 cut(s) 1548, 1549, 1562
BcoDI GTCTC 5 cut(s) 173, 554, 579, 956, 1422
BcuI ACTAGT 1 cut(s) 661
BfmI CTRYAG 2 cut(s) 1609, 1675
BfoI RGCGCY 1 cut(s) 1219
BisI GCNGC 4 cut(s) 109, 996, 1164, 1721
BlsI GCNGC 4 cut(s) 110, 997, 1165, 1722
Bme1390I CCNGG 5 cut(s) 819, 850, 1548, 1549, 1562
Bme18I GGWCC 2 cut(s) 529, 1735
BmeT110I CYCGRG 3 cut(s) 176, 1547, 1724
BmgT120I GGNCC 4 cut(s) 529, 1038, 1558, 1735
BmiI GGNNCC 6 cut(s) 251, 531, 620, 1217, 1307, 1560
BmrFI CCNGG 5 cut(s) 819, 850, 1548, 1549, 1562
BmrI ACTGGG 2 cut(s) 248, 527
BmsI GCATC 6 cut(s) 215, 746, 1113, 1341, 1385, 1440
BmuI ACTGGG 2 cut(s) 248, 527
BpiI GAAGAC 2 cut(s) 261, 975
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 3 cut(s) 357, 1534, 1826
BpuEI CTTGAG 2 cut(s) 181, 557
BpuMI CCSGG 3 cut(s) 1548, 1549, 1562
BsaHI GRCGYC 1 cut(s) 1216
BsaJI CCNNGG 6 cut(s) 818, 848, 1246, 1547, 1560, 1904
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 778, 1176, 1538, 1600
Bse118I RCCGGY 1 cut(s) 1538
Bse1I ACTGG 5 cut(s) 254, 328, 533, 748, 1980
Bse3DI GCAATG 2 cut(s) 501, 1382
BseBI CCWGG 2 cut(s) 819, 850
BseDI CCNNGG 6 cut(s) 818, 848, 1246, 1547, 1560, 1904
BseGI GGATG 3 cut(s) 1332, 1609, 1976
BseLI CCNNNNNNNGG 5 cut(s) 259, 778, 1176, 1538, 1600
BseMI GCAATG 2 cut(s) 501, 1382
BseMII CTCAG 5 cut(s) 371, 642, 747, 1058, 1840
BseNI ACTGG 5 cut(s) 254, 328, 533, 748, 1980
BseRI GAGGAG 2 cut(s) 87, 366
BseXI GCAGC 4 cut(s) 120, 982, 1175, 1707
BsgI GTGCAG 2 cut(s) 1871, 1935
Bsh1236I CGCG 1 cut(s) 1709
Bsh1285I CGRYCG 1 cut(s) 1315
BshFI GGCC 7 cut(s) 905, 1040, 1559, 1797, 1831, 1903, 1909
BshNI GGYRCC 2 cut(s) 249, 1215
BsiEI CGRYCG 1 cut(s) 1315
BsiHKAI GWGCWC 1 cut(s) 716
BsiHKCI CYCGRG 3 cut(s) 176, 1547, 1724
BsiSI CCGG 4 cut(s) 260, 1539, 1548, 1562
BslI CCNNNNNNNGG 5 cut(s) 259, 778, 1176, 1538, 1600
BsmAI GTCTC 5 cut(s) 173, 554, 579, 956, 1422
BsnI GGCC 7 cut(s) 905, 1040, 1559, 1797, 1831, 1903, 1909
BsoBI CYCGRG 3 cut(s) 176, 1547, 1724
Bsp1286I GDGCHC 1 cut(s) 716
Bsp143I GATC 6 cut(s) 64, 137, 416, 484, 1513, 1652
BspACI CCGC 2 cut(s) 218, 1580
BspANI GGCC 7 cut(s) 905, 1040, 1559, 1797, 1831, 1903, 1909
BspCNI CTCAG 5 cut(s) 370, 643, 748, 1057, 1839
BspFNI CGCG 1 cut(s) 1709
BspHI TCATGA 2 cut(s) 468, 1986
BspLI GGNNCC 6 cut(s) 251, 531, 620, 1217, 1307, 1560
BspPI GGATC 4 cut(s) 59, 145, 479, 1508
BspT107I GGYRCC 2 cut(s) 249, 1215
BsrDI GCAATG 2 cut(s) 501, 1382
BsrFI RCCGGY 1 cut(s) 1538
BsrI ACTGG 5 cut(s) 254, 328, 533, 748, 1980
BssAI RCCGGY 1 cut(s) 1538
BssECI CCNNGG 6 cut(s) 818, 848, 1246, 1547, 1560, 1904
BssMI GATC 6 cut(s) 64, 137, 416, 484, 1513, 1652
BssNI GRCGYC 1 cut(s) 1216
BssT1I CCWWGG 2 cut(s) 1246, 1904
Bst2UI CCWGG 2 cut(s) 819, 850
Bst4CI ACNGT 7 cut(s) 295, 557, 701, 865, 950, 1316, 1679
Bst6I CTCTTC 2 cut(s) 20, 1772
BstACI GRCGYC 1 cut(s) 1216
BstC8I GCNNGC 3 cut(s) 643, 1804, 1808
BstDEI CTNAG 6 cut(s) 357, 651, 756, 1044, 1534, 1826
BstF5I GGATG 3 cut(s) 1332, 1609, 1976
BstFNI CGCG 1 cut(s) 1709
BstH2I RGCGCY 1 cut(s) 1219
BstHHI GCGC 1 cut(s) 1218
BstKTI GATC 6 cut(s) 67, 140, 419, 487, 1516, 1655
BstMAI GTCTC 5 cut(s) 173, 554, 579, 956, 1422
BstMBI GATC 6 cut(s) 64, 137, 416, 484, 1513, 1652
BstMCI CGRYCG 1 cut(s) 1315
BstMWI GCNNNNNNNGC 4 cut(s) 1001, 1222, 1586, 1803
BstNI CCWGG 2 cut(s) 819, 850
BstNSI RCATGY 1 cut(s) 1297
BstSCI CCNGG 5 cut(s) 817, 848, 1546, 1547, 1560
BstSFI CTRYAG 2 cut(s) 1609, 1675
BstUI CGCG 1 cut(s) 1709
BstV1I GCAGC 4 cut(s) 120, 982, 1175, 1707
BstV2I GAAGAC 2 cut(s) 261, 975
BstX2I RGATCY 1 cut(s) 1513
BstXI CCANNNNNNTGG 1 cut(s) 953
BstYI RGATCY 1 cut(s) 1513
BsuRI GGCC 7 cut(s) 905, 1040, 1559, 1797, 1831, 1903, 1909
BtsCI GGATG 3 cut(s) 1332, 1609, 1976
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 5 cut(s) 118, 147, 291, 946, 1953
Cac8I GCNNGC 3 cut(s) 643, 1804, 1808
CciI TCATGA 2 cut(s) 468, 1986
CfoI GCGC 1 cut(s) 1218
Cfr10I RCCGGY 1 cut(s) 1538
Cfr13I GGNCC 4 cut(s) 529, 1038, 1558, 1735
Cfr9I CCCGGG 1 cut(s) 1547
Csp6I GTAC 7 cut(s) 250, 1189, 1238, 1354, 1542, 1552, 1705
CviAII CATG 9 cut(s) 133, 264, 469, 476, 691, 1124, 1294, 1397, 1987
CviQI GTAC 7 cut(s) 250, 1189, 1238, 1354, 1542, 1552, 1705
DdeI CTNAG 6 cut(s) 357, 651, 756, 1044, 1534, 1826
DinI GGCGCC 1 cut(s) 1217
DpnI GATC 6 cut(s) 66, 139, 418, 486, 1515, 1654
DpnII GATC 6 cut(s) 64, 137, 416, 484, 1513, 1652
DraIII CACNNNGTG 1 cut(s) 428
Eam1104I CTCTTC 2 cut(s) 20, 1772
EarI CTCTTC 2 cut(s) 20, 1772
Ecl136II GAGCTC 1 cut(s) 714
Eco130I CCWWGG 2 cut(s) 1246, 1904
Eco147I AGGCCT 1 cut(s) 1909
Eco24I GRGCYC 1 cut(s) 716
Eco47I GGWCC 2 cut(s) 529, 1735
Eco53kI GAGCTC 1 cut(s) 714
Eco57I CTGAAG 3 cut(s) 132, 779, 1036
Eco88I CYCGRG 3 cut(s) 176, 1547, 1724
EcoICRI GAGCTC 1 cut(s) 714
EcoRII CCWGG 2 cut(s) 817, 848
EcoT14I CCWWGG 2 cut(s) 1246, 1904
EcoT22I ATGCAT 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 716
EgeI GGCGCC 1 cut(s) 1217
EheI GGCGCC 1 cut(s) 1217
ErhI CCWWGG 2 cut(s) 1246, 1904
FaeI CATG 9 cut(s) 136, 267, 472, 479, 694, 1127, 1297, 1400, 1990
FalI AAGNNNNNCTT 4 cut(s) 1649, 1681, 1731, 1763
FatI CATG 9 cut(s) 132, 263, 468, 475, 690, 1123, 1293, 1396, 1986
FauI CCCGC 1 cut(s) 1573
FblI GTMKAC 1 cut(s) 1095
Fnu4HI GCNGC 4 cut(s) 109, 996, 1164, 1721
FokI GGATG 3 cut(s) 1319, 1616, 1963
FriOI GRGCYC 1 cut(s) 716
Fsp4HI GCNGC 4 cut(s) 109, 996, 1164, 1721
GlaI GCGC 1 cut(s) 1217
GluI GCNGC 4 cut(s) 109, 996, 1164, 1721
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 1219
HaeIII GGCC 7 cut(s) 905, 1040, 1559, 1797, 1831, 1903, 1909
HapII CCGG 4 cut(s) 260, 1539, 1548, 1562
HhaI GCGC 1 cut(s) 1218
Hin1I GRCGYC 1 cut(s) 1216
Hin1II CATG 9 cut(s) 136, 267, 472, 479, 694, 1127, 1297, 1400, 1990
Hin6I GCGC 1 cut(s) 1216
HinP1I GCGC 1 cut(s) 1216
HindIII AAGCTT 1 cut(s) 1520
HinfI GANTC 8 cut(s) 166, 405, 547, 1120, 1457, 1598, 1620, 1950
HpaII CCGG 4 cut(s) 260, 1539, 1548, 1562
HphI GGTGA 5 cut(s) 164, 445, 658, 778, 1493
Hpy166II GTNNAC 5 cut(s) 597, 1017, 1096, 1423, 1735
Hpy188I TCNGA 6 cut(s) 652, 711, 1068, 1325, 1462, 1603
Hpy8I GTNNAC 5 cut(s) 597, 1017, 1096, 1423, 1735
Hpy99I CGWCG 1 cut(s) 1673
HpyAV CCTTC 9 cut(s) 91, 100, 110, 149, 298, 381, 803, 916, 1837
HpyCH4III ACNGT 7 cut(s) 295, 557, 701, 865, 950, 1316, 1679
HpyCH4IV ACGT 1 cut(s) 633
HpyCH4V TGCA 8 cut(s) 108, 132, 539, 998, 1206, 1810, 1852, 1916
HpyF10VI GCNNNNNNNGC 4 cut(s) 1001, 1222, 1586, 1803
HpyF3I CTNAG 6 cut(s) 357, 651, 756, 1044, 1534, 1826
HpySE526I ACGT 1 cut(s) 633
Hsp92I GRCGYC 1 cut(s) 1216
Hsp92II CATG 9 cut(s) 136, 267, 472, 479, 694, 1127, 1297, 1400, 1990
HspAI GCGC 1 cut(s) 1216
KasI GGCGCC 1 cut(s) 1215
KpnI GGTACC 1 cut(s) 253
Kzo9I GATC 6 cut(s) 64, 137, 416, 484, 1513, 1652
LmnI GCTCC 3 cut(s) 719, 1305, 1948
Lsp1109I GCAGC 4 cut(s) 120, 982, 1175, 1707
LweI GCATC 6 cut(s) 215, 746, 1113, 1341, 1385, 1440
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 5 cut(s) 142, 664, 1256, 1316, 1481
MalI GATC 6 cut(s) 66, 139, 418, 486, 1515, 1654
MboI GATC 6 cut(s) 64, 137, 416, 484, 1513, 1652
MfeI CAATTG 2 cut(s) 600, 1209
MflI RGATCY 1 cut(s) 1513
MhlI GDGCHC 1 cut(s) 716
MluCI AATT 7 cut(s) 122, 450, 600, 1209, 1527, 1699, 1757
Mly113I GGCGCC 1 cut(s) 1216
MlyI GAGTC 4 cut(s) 175, 399, 556, 1451
MmeI TCCRAC 1 cut(s) 596
Mph1103I ATGCAT 1 cut(s) 134
MseI TTAA 5 cut(s) 438, 567, 674, 1280, 1364
MslI CAYNNNNRTG 3 cut(s) 467, 951, 1479
MspA1I CMGCKG 2 cut(s) 641, 995
MspI CCGG 4 cut(s) 260, 1539, 1548, 1562
MspR9I CCNGG 5 cut(s) 819, 850, 1548, 1549, 1562
MunI CAATTG 2 cut(s) 600, 1209
MvaI CCWGG 2 cut(s) 819, 850
MvnI CGCG 1 cut(s) 1709
MwoI GCNNNNNNNGC 4 cut(s) 1001, 1222, 1586, 1803
NarI GGCGCC 1 cut(s) 1216
NciI CCSGG 3 cut(s) 1548, 1549, 1562
NdeII GATC 6 cut(s) 64, 137, 416, 484, 1513, 1652
NlaIII CATG 9 cut(s) 136, 267, 472, 479, 694, 1127, 1297, 1400, 1990
NlaIV GGNNCC 6 cut(s) 251, 531, 620, 1217, 1307, 1560
NmuCI GTSAC 4 cut(s) 142, 664, 1256, 1481
NsiI ATGCAT 1 cut(s) 134
NspI RCATGY 1 cut(s) 1297
OliI CACNNNNGTG 1 cut(s) 951
PaeR7I CTCGAG 1 cut(s) 1724
PagI TCATGA 2 cut(s) 468, 1986
PceI AGGCCT 1 cut(s) 1909
PcsI WCGNNNNNNNCGW 3 cut(s) 1308, 1713, 1722
PfeI GAWTC 4 cut(s) 1120, 1598, 1620, 1950
PkrI GCNGC 4 cut(s) 110, 997, 1165, 1722
PleI GAGTC 4 cut(s) 174, 399, 555, 1451
PluTI GGCGCC 1 cut(s) 1219
PpsI GAGTC 4 cut(s) 174, 399, 555, 1451
Psp124BI GAGCTC 1 cut(s) 716
Psp6I CCWGG 2 cut(s) 817, 848
PspGI CCWGG 2 cut(s) 817, 848
PspN4I GGNNCC 6 cut(s) 251, 531, 620, 1217, 1307, 1560
PspPI GGNCC 4 cut(s) 529, 1038, 1558, 1735
PspXI VCTCGAGB 1 cut(s) 1724
PsuI RGATCY 1 cut(s) 1513
PvuII CAGCTG 2 cut(s) 641, 995
RsaI GTAC 7 cut(s) 251, 1190, 1239, 1355, 1543, 1553, 1706
RsaNI GTAC 7 cut(s) 250, 1189, 1238, 1354, 1542, 1552, 1705
RseI CAYNNNNRTG 3 cut(s) 467, 951, 1479
SacI GAGCTC 1 cut(s) 716
SaqAI TTAA 5 cut(s) 438, 567, 674, 1280, 1364
SatI GCNGC 4 cut(s) 109, 996, 1164, 1721
Sau3AI GATC 6 cut(s) 64, 137, 416, 484, 1513, 1652
Sau96I GGNCC 4 cut(s) 529, 1038, 1558, 1735
SchI GAGTC 4 cut(s) 175, 399, 556, 1451
ScrFI CCNGG 5 cut(s) 819, 850, 1548, 1549, 1562
SduI GDGCHC 1 cut(s) 716
SfaNI GCATC 6 cut(s) 215, 746, 1113, 1341, 1385, 1440
SfcI CTRYAG 2 cut(s) 1609, 1675
SfoI GGCGCC 1 cut(s) 1217
Sfr274I CTCGAG 1 cut(s) 1724
SinI GGWCC 2 cut(s) 529, 1735
SlaI CTCGAG 1 cut(s) 1724
SmaI CCCGGG 1 cut(s) 1549
SmiMI CAYNNNNRTG 3 cut(s) 467, 951, 1479
SmlI CTYRAG 3 cut(s) 196, 572, 1724
SmoI CTYRAG 3 cut(s) 196, 572, 1724
SpeI ACTAGT 1 cut(s) 661
Sse9I AATT 7 cut(s) 122, 450, 600, 1209, 1527, 1699, 1757
SseBI AGGCCT 1 cut(s) 1909
SsiI CCGC 2 cut(s) 218, 1580
SspDI GGCGCC 1 cut(s) 1215
SstI GAGCTC 1 cut(s) 716
StuI AGGCCT 1 cut(s) 1909
StyD4I CCNGG 5 cut(s) 817, 848, 1546, 1547, 1560
StyI CCWWGG 2 cut(s) 1246, 1904
TaaI ACNGT 7 cut(s) 295, 557, 701, 865, 950, 1316, 1679
TaiI ACGT 1 cut(s) 636
TaqI TCGA 4 cut(s) 696, 1311, 1668, 1725
TasI AATT 7 cut(s) 122, 450, 600, 1209, 1527, 1699, 1757
TatI WGTACW 1 cut(s) 1237
TfiI GAWTC 4 cut(s) 1120, 1598, 1620, 1950
Tru1I TTAA 5 cut(s) 438, 567, 674, 1280, 1364
Tru9I TTAA 5 cut(s) 438, 567, 674, 1280, 1364
TscAI CASTG 5 cut(s) 118, 147, 298, 953, 1960
TseFI GTSAC 4 cut(s) 142, 664, 1256, 1481
TseI GCWGC 4 cut(s) 108, 995, 1163, 1720
Tsp45I GTSAC 4 cut(s) 142, 664, 1256, 1481
TspDTI ATGAA 7 cut(s) 457, 485, 910, 1112, 1302, 1413, 1877
TspGWI ACGGA 2 cut(s) 706, 761
TspMI CCCGGG 1 cut(s) 1547
TspRI CASTG 5 cut(s) 118, 147, 298, 953, 1960
VpaK11BI GGWCC 2 cut(s) 529, 1735
XapI RAATTY 2 cut(s) 1699, 1757
XceI RCATGY 1 cut(s) 1297
XcmI CCANNNNNNNNNTGG 2 cut(s) 261, 638
XhoI CTCGAG 1 cut(s) 1724
XmaI CCCGGG 1 cut(s) 1547
XmiI GTMKAC 1 cut(s) 1095
Zsp2I ATGCAT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.