Rh6AG389000

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0016269
Physical Location & Seq
Forward (+)
58796538 .. 58799143
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG389000.1

Sequence Viewer

Length: 1563 bp
ATGGGATCAGTGACTTCAGGTGAAGGATATTTGAGTCTCACACCCGAGCAACAAAAGAGCAACTCAAGTTCAGCATCGGTTTTACCGCTATACAAAGTTGGGAGGGTTCTGTATCGGTACCCAGTTCCGGCATGGTCTTCTTTTATCTCCACAACCTTCACTGTCAGGATTTCGGCTTTCTCAAATACAACTGGCTCTGGAGATGGAATGGCATTTGTTTTTGCTCAGGATAGTCGTCCCTCTCCTCCTGATAGCTATGGTTCATTTCTTGGACTCCTAGATAGATCAACACAAGGTGGGGTGATTAAGCAGTTGGGAATTGAGCTAGACACTTTCATGAACCATGAGTTTGATCCAGATGACAACCACATTGCCATTGACACAACAAGCATTATGGACCCAGTTGCAATACAGAGTCTCAACAGTTCAGGCATTAACCTCAAGAGTGGGAGAGACATCAAGTTCACAATTGAGTATAATGGTTGGAACCAAATCCTCCACGTTTCAGCTGGCTACTCTGAGAACCCACTAGTCACCATCTTAAACCATTCAATCTCCATGTTTGACAGTATTGTTCCTAGCTCCGTTTATGTTGGCTTCACAGCATCTACTGGAACTCTCCCTGAGAGCCACCAAGTCCGTGATTGGGTTTTCACCTCCCTTGAGTTGCCAAATCCTAGCCCTCCTGGGAATGACAAAGGGCGTAAGAGAAAGCCCAGGAACATTTGGACTGTTGATATCCCTGTTTTTCTGTGTGTGTTGGTTTTGATGGCCTTCACTTACCCTTTCATTTTGAGAGTTGTGAAAAGAAACCACTGTGGTGGGAATGAGAGAGAAGACATAGAAAGCAGGACAAGAACAGCTGCAAATGCCCCTAAAATGTTTACTTACAAGGAGATTTCAAGGGCCACTCAGGACTTCAGCAAGGAAAATCTGATTGGGGTTGGTGGATTTGGAGTTGTCTACAAAGGCATCATACCAGATAAGATGCATGTTCCTCCTAAAACTATTGCTGTCAAGAAGATTACAGCAACCTCCAAACAAGGTGACTTTGGGTTAGCAAGAGTAATGCTACAAGATCCCAAGCTTACAATTCCCCTAGCCGGTACTCCCGGGTACTTGGCCCCGGAAGTGTTAGGGTTCGCGGGAAAAGCTACCCCAGAATCGGATGTCTATAGCTTTGGAATCGTGGTGCTAGAAGTGGTGTGTGGGAGAAGATCAAAGGGTATCTTCGACGACTACAGTTTAGTAGAAAATGTATGGAATTTGTACGCGAAAGACGAGCTGCTCGAGTGCGTGGACCAAGTAGTGCTTGATGGGAAATTTGAAGTGGAACAAGTGAAGAGGACATTGATTGTTGGCCTTGCTTGCTTGCACACAGATTTTATGCTCAGGCCAAAGATAAGGAAGGTGGTGCAGATTTTGATGAACCCAAATGAGCCTCTACTAGATTTGCCAGATACAAGGCCAAGGCCTAGTGCAGTATATCTATCAGTATCTTCTTCTGCTCCTAGAATCACTGATATTGGCTCTGTTGCCTCATCCAGTATCATGATGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase

Protein Analysis

520

Amino Acids

57.05

Weight (kDa)

6.73

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 5 - 222 3.7e-38 Legume lectin domain
Lectin_L-type_dom PF18483 33 - 214 2.8e-15 Legume lectin beta-barrel domain
Pkinase PF00069 347 - 406 3.4e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 347 - 430 1.9e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016269)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29290
malus_domestica MD08G1148700.v1.1 MD08G1148800.v1.1 MD08G1212600.v1.1 MD15G1124000.v1.1
prunus_persica Prupe.1G479400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298101
rosa_laevigata RLG00000011534
rosa_multiflora Rmu_sc0002800.1_g000004
rosa_rugosa Rorug06G0278900
rosa_samantha Rh6AG389000 Rh6BG397300 Rh6CG403200 Rh6DG389800
rosa_wichuraiana Rw6G033970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 117
AccB1I GGYRCC 1 cut(s) 117
AccI GTMKAC 1 cut(s) 963
AccII CGCG 2 cut(s) 1145, 1274
AciI CCGC 2 cut(s) 86, 1145
AclWI GGATC 3 cut(s) 13, 347, 1073
AcsI RAATTY 2 cut(s) 1264, 1322
AcuI CTGAAG 1 cut(s) 904
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 4 cut(s) 119, 1108, 1118, 1271
AfiI CCNNNNNNNGG 4 cut(s) 127, 646, 1103, 1165
AgsI TTSAA 3 cut(s) 552, 903, 1328
AhlI ACTAGT 1 cut(s) 529
AjnI CCWGG 2 cut(s) 685, 716
AjuI GAANNNNNNNTTGG 2 cut(s) 921, 953
AleI CACNNNNGTG 1 cut(s) 819
AluBI AGCT 9 cut(s) 255, 325, 509, 582, 863, 1087, 1154, 1179, 1285
AluI AGCT 9 cut(s) 255, 325, 509, 582, 863, 1087, 1154, 1179, 1285
Alw26I GTCTC 3 cut(s) 41, 422, 447
AlwI GGATC 3 cut(s) 13, 347, 1073
Ama87I CYCGRG 3 cut(s) 44, 1112, 1289
AoxI GGCC 7 cut(s) 771, 906, 1122, 1360, 1394, 1466, 1472
ApeKI GCWGC 2 cut(s) 863, 1285
ApoI RAATTY 2 cut(s) 1264, 1322
Asp718I GGTACC 1 cut(s) 117
AspS9I GGNCC 4 cut(s) 397, 906, 1123, 1300
AsuC2I CCSGG 3 cut(s) 1113, 1114, 1127
AsuHPI GGTGA 5 cut(s) 32, 313, 526, 646, 1058
AvaI CYCGRG 3 cut(s) 44, 1112, 1289
AvaII GGWCC 2 cut(s) 397, 1300
BanI GGYRCC 1 cut(s) 117
BbsI GAAGAC 2 cut(s) 129, 843
BbvI GCAGC 2 cut(s) 850, 1272
BccI CCATC 4 cut(s) 197, 545, 763, 1310
BciT130I CCWGG 2 cut(s) 687, 718
BcnI CCSGG 3 cut(s) 1113, 1114, 1127
BcoDI GTCTC 3 cut(s) 41, 422, 447
BcuI ACTAGT 1 cut(s) 529
BfmI CTRYAG 2 cut(s) 1174, 1240
BisI GCNGC 2 cut(s) 864, 1286
BlsI GCNGC 2 cut(s) 865, 1287
Bme1390I CCNGG 5 cut(s) 687, 718, 1113, 1114, 1127
Bme18I GGWCC 2 cut(s) 397, 1300
BmeT110I CYCGRG 3 cut(s) 44, 1112, 1289
BmgT120I GGNCC 4 cut(s) 397, 906, 1123, 1300
BmiI GGNNCC 4 cut(s) 119, 399, 488, 1125
BmrFI CCNGG 5 cut(s) 687, 718, 1113, 1114, 1127
BmrI ACTGGG 2 cut(s) 116, 395
BmsI GCATC 4 cut(s) 83, 614, 978, 981
BmuI ACTGGG 2 cut(s) 116, 395
BpiI GAAGAC 2 cut(s) 129, 843
BpmI CTGGAG 1 cut(s) 219
Bpu10I CCTNAGC 2 cut(s) 225, 1391
BpuEI CTTGAG 3 cut(s) 49, 425, 683
BpuMI CCSGG 3 cut(s) 1113, 1114, 1127
BsaJI CCNNGG 5 cut(s) 686, 716, 1112, 1125, 1469
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 646, 1103, 1165
Bse118I RCCGGY 1 cut(s) 1103
Bse1I ACTGG 5 cut(s) 122, 196, 401, 616, 1545
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 2 cut(s) 687, 718
BseDI CCNNGG 5 cut(s) 686, 716, 1112, 1125, 1469
BseGI GGATG 2 cut(s) 1174, 1541
BseLI CCNNNNNNNGG 4 cut(s) 127, 646, 1103, 1165
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 5 cut(s) 239, 510, 615, 926, 1405
BseNI ACTGG 5 cut(s) 122, 196, 401, 616, 1545
BseRI GAGGAG 1 cut(s) 234
BseXI GCAGC 2 cut(s) 850, 1272
BsgI GTGCAG 2 cut(s) 1436, 1500
Bsh1236I CGCG 2 cut(s) 1145, 1274
BshFI GGCC 7 cut(s) 773, 908, 1124, 1362, 1396, 1468, 1474
BshNI GGYRCC 1 cut(s) 117
BsiHKCI CYCGRG 3 cut(s) 44, 1112, 1289
BsiSI CCGG 4 cut(s) 128, 1104, 1113, 1127
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 4 cut(s) 127, 646, 1103, 1165
BsmAI GTCTC 3 cut(s) 41, 422, 447
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 7 cut(s) 773, 908, 1124, 1362, 1396, 1468, 1474
BsoBI CYCGRG 3 cut(s) 44, 1112, 1289
Bsp143I GATC 5 cut(s) 5, 284, 352, 1078, 1217
BspACI CCGC 2 cut(s) 86, 1145
BspANI GGCC 7 cut(s) 773, 908, 1124, 1362, 1396, 1468, 1474
BspCNI CTCAG 5 cut(s) 238, 511, 616, 925, 1404
BspFNI CGCG 2 cut(s) 1145, 1274
BspHI TCATGA 2 cut(s) 336, 1551
BspLI GGNNCC 4 cut(s) 119, 399, 488, 1125
BspPI GGATC 3 cut(s) 13, 347, 1073
BspT107I GGYRCC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 1103
BsrI ACTGG 5 cut(s) 122, 196, 401, 616, 1545
BssAI RCCGGY 1 cut(s) 1103
BssECI CCNNGG 5 cut(s) 686, 716, 1112, 1125, 1469
BssMI GATC 5 cut(s) 5, 284, 352, 1078, 1217
BssT1I CCWWGG 1 cut(s) 1469
Bst2UI CCWGG 2 cut(s) 687, 718
Bst4CI ACNGT 6 cut(s) 163, 425, 569, 733, 818, 1244
Bst6I CTCTTC 1 cut(s) 1337
BstC8I GCNNGC 3 cut(s) 511, 1369, 1373
BstDEI CTNAG 5 cut(s) 225, 519, 624, 912, 1391
BstF5I GGATG 2 cut(s) 1174, 1541
BstFNI CGCG 2 cut(s) 1145, 1274
BstKTI GATC 5 cut(s) 8, 287, 355, 1081, 1220
BstMAI GTCTC 3 cut(s) 41, 422, 447
BstMBI GATC 5 cut(s) 5, 284, 352, 1078, 1217
BstMWI GCNNNNNNNGC 3 cut(s) 869, 1151, 1368
BstNI CCWGG 2 cut(s) 687, 718
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 5 cut(s) 685, 716, 1111, 1112, 1125
BstSFI CTRYAG 2 cut(s) 1174, 1240
BstUI CGCG 2 cut(s) 1145, 1274
BstV1I GCAGC 2 cut(s) 850, 1272
BstV2I GAAGAC 2 cut(s) 129, 843
BstX2I RGATCY 1 cut(s) 1078
BstXI CCANNNNNNTGG 1 cut(s) 821
BstYI RGATCY 1 cut(s) 1078
BsuRI GGCC 7 cut(s) 773, 908, 1124, 1362, 1396, 1468, 1474
BtsCI GGATG 2 cut(s) 1174, 1541
BtsIMutI CAGTG 4 cut(s) 15, 159, 814, 1518
Cac8I GCNNGC 3 cut(s) 511, 1369, 1373
CciI TCATGA 2 cut(s) 336, 1551
Cfr10I RCCGGY 1 cut(s) 1103
Cfr13I GGNCC 4 cut(s) 397, 906, 1123, 1300
Cfr9I CCCGGG 1 cut(s) 1112
Csp6I GTAC 4 cut(s) 118, 1107, 1117, 1270
CviAII CATG 6 cut(s) 132, 337, 344, 559, 992, 1552
CviQI GTAC 4 cut(s) 118, 1107, 1117, 1270
DdeI CTNAG 5 cut(s) 225, 519, 624, 912, 1391
DpnI GATC 5 cut(s) 7, 286, 354, 1080, 1219
DpnII GATC 5 cut(s) 5, 284, 352, 1078, 1217
DraIII CACNNNGTG 1 cut(s) 296
Eam1104I CTCTTC 1 cut(s) 1337
EarI CTCTTC 1 cut(s) 1337
Eco130I CCWWGG 1 cut(s) 1469
Eco147I AGGCCT 1 cut(s) 1474
Eco32I GATATC 1 cut(s) 739
Eco47I GGWCC 2 cut(s) 397, 1300
Eco57I CTGAAG 1 cut(s) 904
Eco88I CYCGRG 3 cut(s) 44, 1112, 1289
EcoRII CCWGG 2 cut(s) 685, 716
EcoRV GATATC 1 cut(s) 739
EcoT14I CCWWGG 1 cut(s) 1469
EcoT22I ATGCAT 1 cut(s) 993
ErhI CCWWGG 1 cut(s) 1469
FaeI CATG 6 cut(s) 135, 340, 347, 562, 995, 1555
FalI AAGNNNNNCTT 4 cut(s) 1214, 1246, 1296, 1328
FaqI GGGAC 1 cut(s) 222
FatI CATG 6 cut(s) 131, 336, 343, 558, 991, 1551
FauI CCCGC 1 cut(s) 1138
FblI GTMKAC 1 cut(s) 963
Fnu4HI GCNGC 2 cut(s) 864, 1286
FokI GGATG 2 cut(s) 1181, 1528
Fsp4HI GCNGC 2 cut(s) 864, 1286
GluI GCNGC 2 cut(s) 864, 1286
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 7 cut(s) 773, 908, 1124, 1362, 1396, 1468, 1474
HapII CCGG 4 cut(s) 128, 1104, 1113, 1127
Hin1II CATG 6 cut(s) 135, 340, 347, 562, 995, 1555
HindIII AAGCTT 1 cut(s) 1085
HinfI GANTC 6 cut(s) 34, 273, 415, 1163, 1185, 1515
HpaII CCGG 4 cut(s) 128, 1104, 1113, 1127
HphI GGTGA 5 cut(s) 32, 313, 526, 646, 1058
Hpy166II GTNNAC 4 cut(s) 465, 885, 964, 1300
Hpy188I TCNGA 3 cut(s) 520, 936, 1168
Hpy8I GTNNAC 4 cut(s) 465, 885, 964, 1300
Hpy99I CGWCG 1 cut(s) 1238
HpyAV CCTTC 4 cut(s) 17, 166, 784, 1402
HpyCH4III ACNGT 6 cut(s) 163, 425, 569, 733, 818, 1244
HpyCH4IV ACGT 1 cut(s) 501
HpyCH4V TGCA 6 cut(s) 407, 866, 991, 1375, 1417, 1481
HpyF10VI GCNNNNNNNGC 3 cut(s) 869, 1151, 1368
HpyF3I CTNAG 5 cut(s) 225, 519, 624, 912, 1391
HpySE526I ACGT 1 cut(s) 501
Hsp92II CATG 6 cut(s) 135, 340, 347, 562, 995, 1555
KpnI GGTACC 1 cut(s) 121
Kzo9I GATC 5 cut(s) 5, 284, 352, 1078, 1217
LmnI GCTCC 2 cut(s) 587, 1513
Lsp1109I GCAGC 2 cut(s) 850, 1272
LweI GCATC 4 cut(s) 83, 614, 978, 981
MaeII ACGT 1 cut(s) 501
MaeIII GTNAC 3 cut(s) 10, 532, 1046
MalI GATC 5 cut(s) 7, 286, 354, 1080, 1219
MboI GATC 5 cut(s) 5, 284, 352, 1078, 1217
MboII GAAGA 8 cut(s) 129, 848, 1033, 1222, 1227, 1354, 1491, 1494
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 1 cut(s) 1078
MluCI AATT 5 cut(s) 318, 468, 1092, 1264, 1322
MlyI GAGTC 3 cut(s) 43, 267, 424
MmeI TCCRAC 1 cut(s) 464
Mph1103I ATGCAT 1 cut(s) 993
MseI TTAA 3 cut(s) 306, 435, 542
MslI CAYNNNNRTG 2 cut(s) 335, 819
MspA1I CMGCKG 2 cut(s) 509, 863
MspI CCGG 4 cut(s) 128, 1104, 1113, 1127
MspR9I CCNGG 5 cut(s) 687, 718, 1113, 1114, 1127
MunI CAATTG 1 cut(s) 468
MvaI CCWGG 2 cut(s) 687, 718
MvnI CGCG 2 cut(s) 1145, 1274
MwoI GCNNNNNNNGC 3 cut(s) 869, 1151, 1368
NciI CCSGG 3 cut(s) 1113, 1114, 1127
NdeII GATC 5 cut(s) 5, 284, 352, 1078, 1217
NlaIII CATG 6 cut(s) 135, 340, 347, 562, 995, 1555
NlaIV GGNNCC 4 cut(s) 119, 399, 488, 1125
NmuCI GTSAC 3 cut(s) 10, 532, 1046
NsiI ATGCAT 1 cut(s) 993
NspI RCATGY 1 cut(s) 995
OliI CACNNNNGTG 1 cut(s) 819
PaeR7I CTCGAG 1 cut(s) 1289
PagI TCATGA 2 cut(s) 336, 1551
PceI AGGCCT 1 cut(s) 1474
PcsI WCGNNNNNNNCGW 2 cut(s) 1278, 1287
PfeI GAWTC 3 cut(s) 1163, 1185, 1515
PkrI GCNGC 2 cut(s) 865, 1287
PleI GAGTC 3 cut(s) 42, 267, 423
PpsI GAGTC 3 cut(s) 42, 267, 423
Psp6I CCWGG 2 cut(s) 685, 716
PspGI CCWGG 2 cut(s) 685, 716
PspN4I GGNNCC 4 cut(s) 119, 399, 488, 1125
PspPI GGNCC 4 cut(s) 397, 906, 1123, 1300
PspXI VCTCGAGB 1 cut(s) 1289
PsuI RGATCY 1 cut(s) 1078
PvuII CAGCTG 2 cut(s) 509, 863
RsaI GTAC 4 cut(s) 119, 1108, 1118, 1271
RsaNI GTAC 4 cut(s) 118, 1107, 1117, 1270
RseI CAYNNNNRTG 2 cut(s) 335, 819
SaqAI TTAA 3 cut(s) 306, 435, 542
SatI GCNGC 2 cut(s) 864, 1286
Sau3AI GATC 5 cut(s) 5, 284, 352, 1078, 1217
Sau96I GGNCC 4 cut(s) 397, 906, 1123, 1300
SchI GAGTC 3 cut(s) 43, 267, 424
ScrFI CCNGG 5 cut(s) 687, 718, 1113, 1114, 1127
SfaNI GCATC 4 cut(s) 83, 614, 978, 981
SfcI CTRYAG 2 cut(s) 1174, 1240
Sfr274I CTCGAG 1 cut(s) 1289
SinI GGWCC 2 cut(s) 397, 1300
SlaI CTCGAG 1 cut(s) 1289
SmaI CCCGGG 1 cut(s) 1114
SmiMI CAYNNNNRTG 2 cut(s) 335, 819
SmlI CTYRAG 4 cut(s) 64, 440, 662, 1289
SmoI CTYRAG 4 cut(s) 64, 440, 662, 1289
SpeI ACTAGT 1 cut(s) 529
Sse9I AATT 5 cut(s) 318, 468, 1092, 1264, 1322
SseBI AGGCCT 1 cut(s) 1474
SsiI CCGC 2 cut(s) 86, 1145
StuI AGGCCT 1 cut(s) 1474
StyD4I CCNGG 5 cut(s) 685, 716, 1111, 1112, 1125
StyI CCWWGG 1 cut(s) 1469
TaaI ACNGT 6 cut(s) 163, 425, 569, 733, 818, 1244
TaiI ACGT 1 cut(s) 504
TaqI TCGA 2 cut(s) 1233, 1290
TasI AATT 5 cut(s) 318, 468, 1092, 1264, 1322
TfiI GAWTC 3 cut(s) 1163, 1185, 1515
Tru1I TTAA 3 cut(s) 306, 435, 542
Tru9I TTAA 3 cut(s) 306, 435, 542
TscAI CASTG 4 cut(s) 15, 166, 821, 1525
TseFI GTSAC 3 cut(s) 10, 532, 1046
TseI GCWGC 2 cut(s) 863, 1285
Tsp45I GTSAC 3 cut(s) 10, 532, 1046
TspDTI ATGAA 5 cut(s) 252, 325, 353, 778, 1442
TspGWI ACGGA 2 cut(s) 574, 629
TspMI CCCGGG 1 cut(s) 1112
TspRI CASTG 4 cut(s) 15, 166, 821, 1525
VpaK11BI GGWCC 2 cut(s) 397, 1300
XapI RAATTY 2 cut(s) 1264, 1322
XceI RCATGY 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 2 cut(s) 129, 506
XhoI CTCGAG 1 cut(s) 1289
XmaI CCCGGG 1 cut(s) 1112
XmiI GTMKAC 1 cut(s) 963
Zsp2I ATGCAT 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.