Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0016478

Physical Location & Seq

Genomic Coordinates Forward (+)

64639624 .. 64643256

Gene Structure 3633 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ27476

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2226 bp

ATGTCTTCACCGCCGTGGTCTCTGCCTTCGGCTGCCGTTCCTGTCACCACACCACCACCACCACCACTTCCACCGTCACAGCCACCGGCATTGCCTCCACCATCGCCACAGTCACCACCTTTTGTATCCCCGCTATTTCCACCTACTAGTCTTCCTGCCCTGCCTCCTGTACACAACTCTATCCCTCCTGTTTCCTCACCATTGCCATCTGTGGTCCAACCTCCACCCCCACCAGTCACCTCTCCACCACCGCCGTCTGTCACTTCTCCTCCTCCACCTTCACTAACCTTTCCGCCACCACCTTCACTACCGCCACCAACTCCATTGCCATCAAGACCTTCTCCACCTCCACCTCCACCTCCACAGCCTACATTATCACCACCACCATTACCAACACCAAAAACACCATATCTTTCTCCTCCAATCAAACCACTAACTTCTCCTCCGCTGCCACCACCCTCAACAACTCCAACCAACGACTCTCCACCACCTACATTGTCAACTCCCCTACCTCCGGTGACCACCACTACTCCTCCACCTCCTTTAGCCACTCCAAGCAACAGCGCTCCACCACCTGATTTAGCAACTCTGCTTCCACCAGTGGCCACCAGTCCTAAACCACCACCCACTTTGGCCAGAACTCTGCCACCTTCGCCTAGTAATGCTGTAACACCTTTGCCTAGTAATGCTGTAACACCTTTGCCTACAACAACATTGACACCACCTCCATTGATCCATTCACCATCACCGTCCTTATTGCCGGCCACCTTGCAAGTTCAGAAGCCACAGCCAAAAGCAGCAGCAGGCCTCGTAGCTGGATGTGTCATTGTTGGAGTTGTTGTAGTTTTCCTCCTCCTCGGAATTGTACTTATTTGCTACAAGCGTAGGAGTAGAAAGAACAACGCTATTCCGGAAGATTACTATAAACCACCCTCCTTGGGACCAAAAGATGGCTGTTATGATGCGGTTCAGCCCCATCACAATGTTCCTCAACCTGGCGTCCATGTTATCAGGGTGCTGTCCAGCACATCAACATCTCCAATGTGCCGTGCCGCTGGCTCTGAGGTTTTATATGCACATCAACGTCAATGGCTTGGTTTTCCCATGGACATCTCAAATGGAACATTCACCTATGATCAATTGTTGGTAGCAACAAATGGGTTTTCAGAGGCCAACCTTCTTGGACAAGGCGGTTTTGGGTATGTCCACAAGGGAGTACTTCCAGGTGGGAAGGAAGTTGCAGTGAAGCAGTTAAAGACAGGAAGCCGGCAAGGGGAGCGAGAGTTTCATGCTGAGGTTGACATCATTAGCCGGGTGCATCACAAGCATCTAGTTTCATTGGTAGGGCACTGTATCAAGGGGGCTGAGAGGTTGCTTGTATATGAGTTTGTTCCAAATAACACCTTAGAATTCCACTTGCATGGATTAGGACAGACTGTTTTGGAGTGGGAAATAAGATTGAAAATAGCTACTGGCTCTGCAAAAGGACTAGCATATCTCCATGAAGATTGCAATCCAAAAATCATTCATCGTGATATCAAGGCATCTAATATTCTTGTGGATCAGAAGTTTGAAGCAAAGGTTTCTGACTTTGGACTGGCCAAATCGTTCTCTGGTACCAACACTCACATTACTCACCTTTCCACTCGAGTAGTGGGAACATTTGGGTACCTTGCTCCAGAGTACGCATCAAGCGGTAAGGTCACAGAGAAATCAGATGTGTATTCATACGGGGTTGTACTCCTAGAACTTATAACCGGACGTCCGCCTATCAGTACAATAGATTCCTTGAGGAATGAGGGTCTGGTTCAGTGGGCAAGGCCATTGCTCACTCAAGCCCTGGAAGATGGTGCTTTTGATGCTCTTGTGGATCCACGATTGGAGACAAATTACAAGAAGAATGAGATGGCTAGAATGGTCGCTTGTGCTGCTGCGTGTGTTCGACATTCAGCATGGCTTCGACCAAGAATGAGCCAGATAGTCCATGCTTTAGAAGGAGTTGCTTCGTTGATGGATCTTCAGGAAGGAGTTACACCAGGAAACAGTGCGGTATACAATTGCTTGGGGAATTCACATTATAACTCCCGGCAATGTGAGGAAGATTTGAAGAATTCCACGGTAACAATGCCAAGTCAAGAGTATGGCATTAGTAGATACACTGCAACTACCAGTGAGTATGGTCTTAATCCCTCAGGCTCAAGCAGCGAAACCCGGCAAACAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

741

Amino Acids

78.82

Weight (kDa)

8.65

Isoelectric Point (pI)

65.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	390 - 595	3e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	392 - 664	1.2e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32710
fragaria_vesca	FvH4_2g36150
malus_domestica	MD15G1098100.v1.1
prunus_persica	Prupe.1G452900_v2.0.a1 Prupe.1G452900_v2.0.a1
pyrus_communis	pycom15g08990
rosa_chinensis	RchiOBHm_Chr6g0305721
rosa_laevigata	RLG00000010865
rosa_multiflora	Rmu_sc0001809.1_g000037
rosa_roxburghii	Rroxscaffold_7G00162410
rosa_rugosa	Rorug06G0343500
rosa_samantha	Rh6BG446400 Rh6CG468900
rosa_wichuraiana	Rw6G039690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1754, 2079
AatII	GACGTC	1 cut(s)	1765
Acc65I	GGTACC	2 cut(s)	1616, 1668
AccB1I	GGYRCC	2 cut(s)	1616, 1668
AccB7I	CCANNNNNTGG	1 cut(s)	950
AccI	GTMKAC	1 cut(s)	2052
AccIII	TCCGGA	1 cut(s)	910
AclWI	GGATC	5 cut(s)	727, 1569, 1865, 1878, 2022
AcoI	YGGCCR	4 cut(s)	603, 633, 762, 1599
AcsI	RAATTY	3 cut(s)	1409, 2068, 2110
AcuI	CTGAAG	1 cut(s)	2003
AcyI	GRCGYC	2 cut(s)	999, 1762
AfaI	GTAC	8 cut(s)	171, 867, 1218, 1618, 1670, 1685, 1740, 1777
AfeI	AGCGCT	1 cut(s)	565
AfiI	CCNNNNNNNGG	5 cut(s)	514, 938, 950, 995, 1273
AgsI	TTSAA	3 cut(s)	1462, 1574, 2107
AhlI	ACTAGT	1 cut(s)	146
AjnI	CCWGG	4 cut(s)	994, 1222, 1839, 2035
AleI	CACNNNNGTG	1 cut(s)	13
AluBI	AGCT	2 cut(s)	815, 1469
AluI	AGCT	2 cut(s)	815, 1469
Alw26I	GTCTC	2 cut(s)	24, 1877
AlwI	GGATC	5 cut(s)	727, 1569, 1865, 1878, 2022
Ama87I	CYCGRG	1 cut(s)	1647
Aor13HI	TCCGGA	1 cut(s)	910
Aor51HI	AGCGCT	1 cut(s)	565
AoxI	GGCC	7 cut(s)	603, 633, 762, 805, 1170, 1599, 1820
ApeKI	GCWGC	7 cut(s)	32, 448, 797, 800, 1928, 1931, 2202
ApoI	RAATTY	3 cut(s)	1409, 2068, 2110
Asp718I	GGTACC	2 cut(s)	1616, 1668
AspLEI	GCGC	1 cut(s)	566
AspS9I	GGNCC	2 cut(s)	214, 941
AsuC2I	CCSGG	3 cut(s)	1313, 2086, 2212
AvaI	CYCGRG	1 cut(s)	1647
AvaII	GGWCC	2 cut(s)	214, 941
AxyI	CCTNAGG	1 cut(s)	2191
BaeGI	GKGCMC	1 cut(s)	1350
BalI	TGGCCA	3 cut(s)	605, 635, 1601
BamHI	GGATCC	1 cut(s)	1870
BanI	GGYRCC	2 cut(s)	1616, 1668
BbsI	GAAGAC	1 cut(s)	143
BbvCI	CCTCAGC	1 cut(s)	1293
BbvI	GCAGC	7 cut(s)	19, 435, 809, 812, 1915, 1918, 2214
BccI	CCATC	9 cut(s)	109, 214, 337, 751, 944, 984, 1841, 1900, 2005
BceAI	ACGGC	3 cut(s)	20, 238, 1032
BciT130I	CCWGG	4 cut(s)	996, 1224, 1841, 2037
BciVI	GTATCC	1 cut(s)	136
BclI	TGATCA	1 cut(s)	1135
BcnI	CCSGG	3 cut(s)	1313, 2086, 2212
BcoDI	GTCTC	2 cut(s)	24, 1877
BcuI	ACTAGT	1 cut(s)	146
BfaI	CTAG	7 cut(s)	147, 657, 681, 1331, 1490, 1745, 1911
BfoI	RGCGCY	1 cut(s)	567
BfuI	GTATCC	1 cut(s)	136
BisI	GCNGC	8 cut(s)	33, 449, 798, 801, 1053, 1929, 1932, 2203
BlsI	GCNGC	8 cut(s)	34, 450, 799, 802, 1054, 1930, 1933, 2204
BmcAI	AGTACT	1 cut(s)	1218
Bme1390I	CCNGG	7 cut(s)	996, 1224, 1313, 1841, 2037, 2086, 2212
Bme18I	GGWCC	2 cut(s)	214, 941
BmeT110I	CYCGRG	1 cut(s)	1647
BmgT120I	GGNCC	2 cut(s)	214, 941
BmiI	GGNNCC	4 cut(s)	942, 1618, 1670, 1872
BmrFI	CCNGG	7 cut(s)	996, 1224, 1313, 1841, 2037, 2086, 2212
BmsI	GCATC	6 cut(s)	952, 1327, 1336, 1553, 1697, 1849
BpiI	GAAGAC	1 cut(s)	143
BpmI	CTGGAG	1 cut(s)	1662
Bpu10I	CCTNAGC	1 cut(s)	1293
BpuEI	CTTGAG	3 cut(s)	1810, 1818, 2182
BpuMI	CCSGG	3 cut(s)	1313, 2086, 2212
BsaBI	GATNNNNATC	1 cut(s)	1512
BsaHI	GRCGYC	2 cut(s)	999, 1762
BsaI	GGTCTC	1 cut(s)	24
BsaJI	CCNNGG	6 cut(s)	14, 856, 936, 1104, 1839, 2115
BsaWI	WCCGGW	3 cut(s)	514, 910, 1757
Bsc4I	CCNNNNNNNGG	5 cut(s)	514, 938, 950, 995, 1273
Bse118I	RCCGGY	3 cut(s)	85, 760, 1266
Bse1I	ACTGG	6 cut(s)	233, 599, 609, 1477, 1602, 2169
Bse21I	CCTNAGG	1 cut(s)	2191
Bse3DI	GCAATG	5 cut(s)	89, 200, 323, 1823, 2096
Bse8I	GATNNNNATC	1 cut(s)	1512
BseAI	TCCGGA	1 cut(s)	910
BseBI	CCWGG	4 cut(s)	996, 1224, 1841, 2037
BseDI	CCNNGG	6 cut(s)	14, 856, 936, 1104, 1839, 2115
BseGI	GGATG	1 cut(s)	824
BseJI	GATNNNNATC	1 cut(s)	1512
BseLI	CCNNNNNNNGG	5 cut(s)	514, 938, 950, 995, 1273
BseMI	GCAATG	5 cut(s)	89, 200, 323, 1823, 2096
BseMII	CTCAG	4 cut(s)	1053, 1284, 1356, 2205
BseNI	ACTGG	6 cut(s)	233, 599, 609, 1477, 1602, 2169
BseRI	GAGGAG	7 cut(s)	258, 261, 408, 432, 522, 842, 845
BseSI	GKGCMC	1 cut(s)	1350
BseXI	GCAGC	7 cut(s)	19, 435, 809, 812, 1915, 1918, 2214
BshFI	GGCC	7 cut(s)	605, 635, 764, 807, 1172, 1601, 1822
BshNI	GGYRCC	2 cut(s)	1616, 1668
BsiHKCI	CYCGRG	1 cut(s)	1647
BsiSI	CCGG	9 cut(s)	86, 515, 761, 911, 1267, 1312, 1758, 2086, 2212
BslFI	GGGAC	1 cut(s)	954
BslI	CCNNNNNNNGG	5 cut(s)	514, 938, 950, 995, 1273
BsmAI	GTCTC	2 cut(s)	24, 1877
BsmFI	GGGAC	1 cut(s)	954
BsnI	GGCC	7 cut(s)	605, 635, 764, 807, 1172, 1601, 1822
Bso31I	GGTCTC	1 cut(s)	24
BsoBI	CYCGRG	1 cut(s)	1647
Bsp1286I	GDGCHC	1 cut(s)	1350
Bsp13I	TCCGGA	1 cut(s)	910
Bsp1407I	TGTACA	1 cut(s)	169
Bsp143I	GATC	5 cut(s)	732, 1135, 1561, 1870, 2014
Bsp19I	CCATGG	1 cut(s)	1104
BspANI	GGCC	7 cut(s)	605, 635, 764, 807, 1172, 1601, 1822
BspCNI	CTCAG	4 cut(s)	1054, 1285, 1357, 2204
BspEI	TCCGGA	1 cut(s)	910
BspLI	GGNNCC	4 cut(s)	942, 1618, 1670, 1872
BspPI	GGATC	5 cut(s)	727, 1569, 1865, 1878, 2022
BspT107I	GGYRCC	2 cut(s)	1616, 1668
BspTNI	GGTCTC	1 cut(s)	24
BsrDI	GCAATG	5 cut(s)	89, 200, 323, 1823, 2096
BsrFI	RCCGGY	3 cut(s)	85, 760, 1266
BsrGI	TGTACA	1 cut(s)	169
BsrI	ACTGG	6 cut(s)	233, 599, 609, 1477, 1602, 2169
BssAI	RCCGGY	3 cut(s)	85, 760, 1266
BssECI	CCNNGG	6 cut(s)	14, 856, 936, 1104, 1839, 2115
BssMI	GATC	5 cut(s)	732, 1135, 1561, 1870, 2014
BssNAI	GTATAC	1 cut(s)	2053
BssNI	GRCGYC	2 cut(s)	999, 1762
BssT1I	CCWWGG	2 cut(s)	936, 1104
Bst1107I	GTATAC	1 cut(s)	2053
Bst2UI	CCWGG	4 cut(s)	996, 1224, 1841, 2037
Bst4CI	ACNGT	7 cut(s)	75, 111, 750, 1352, 1438, 2045, 2119
BstACI	GRCGYC	2 cut(s)	999, 1762
BstAUI	TGTACA	1 cut(s)	169
BstC8I	GCNNGC	4 cut(s)	762, 805, 1057, 1268
BstDEI	CTNAG	5 cut(s)	1062, 1293, 1365, 1405, 2191
BstDSI	CCRYGG	3 cut(s)	14, 1104, 2115
BstEII	GGTNACC	1 cut(s)	517
BstF5I	GGATG	1 cut(s)	824
BstH2I	RGCGCY	1 cut(s)	567
BstHHI	GCGC	1 cut(s)	566
BstKTI	GATC	5 cut(s)	735, 1138, 1564, 1873, 2017
BstMAI	GTCTC	2 cut(s)	24, 1877
BstMBI	GATC	5 cut(s)	732, 1135, 1561, 1870, 2014
BstMWI	GCNNNNNNNGC	6 cut(s)	652, 1276, 1324, 1859, 1928, 2202
BstNI	CCWGG	4 cut(s)	996, 1224, 1841, 2037
BstPI	GGTNACC	1 cut(s)	517
BstSCI	CCNGG	7 cut(s)	994, 1222, 1311, 1839, 2035, 2084, 2210
BstSLI	GKGCMC	1 cut(s)	1350
BstV1I	GCAGC	7 cut(s)	19, 435, 809, 812, 1915, 1918, 2214
BstV2I	GAAGAC	1 cut(s)	143
BstX2I	RGATCY	2 cut(s)	1870, 2014
BstXI	CCANNNNNNTGG	1 cut(s)	1421
BstYI	RGATCY	2 cut(s)	1870, 2014
BstZ17I	GTATAC	1 cut(s)	2053
Bsu36I	CCTNAGG	1 cut(s)	2191
BsuI	GTATCC	1 cut(s)	136
BsuRI	GGCC	7 cut(s)	605, 635, 764, 807, 1172, 1601, 1822
BtgI	CCRYGG	3 cut(s)	14, 1104, 2115
BtgZI	GCGATG	1 cut(s)	87
BtsCI	GGATG	1 cut(s)	824
BtsI	GCAGTG	2 cut(s)	1248, 2157
BtsIMutI	CAGTG	7 cut(s)	606, 1248, 1348, 1817, 2050, 2157, 2176
Cac8I	GCNNGC	4 cut(s)	762, 805, 1057, 1268
CfoI	GCGC	1 cut(s)	566
Cfr10I	RCCGGY	3 cut(s)	85, 760, 1266
Cfr13I	GGNCC	2 cut(s)	214, 941
CseI	GACGC	1 cut(s)	988
Csp6I	GTAC	8 cut(s)	170, 866, 1217, 1617, 1669, 1684, 1739, 1776
CviAII	CATG	7 cut(s)	1004, 1105, 1289, 1421, 1502, 1953, 1985
CviQI	GTAC	8 cut(s)	170, 866, 1217, 1617, 1669, 1684, 1739, 1776
DdeI	CTNAG	5 cut(s)	1062, 1293, 1365, 1405, 2191
DpnI	GATC	5 cut(s)	734, 1137, 1563, 1872, 2016
DpnII	GATC	5 cut(s)	732, 1135, 1561, 1870, 2014
EaeI	YGGCCR	4 cut(s)	603, 633, 762, 1599
EciI	GGCGGA	2 cut(s)	282, 1755
Eco130I	CCWWGG	2 cut(s)	936, 1104
Eco147I	AGGCCT	1 cut(s)	807
Eco31I	GGTCTC	1 cut(s)	24
Eco32I	GATATC	1 cut(s)	1537
Eco47I	GGWCC	2 cut(s)	214, 941
Eco47III	AGCGCT	1 cut(s)	565
Eco57I	CTGAAG	1 cut(s)	2003
Eco81I	CCTNAGG	1 cut(s)	2191
Eco88I	CYCGRG	1 cut(s)	1647
Eco91I	GGTNACC	1 cut(s)	517
EcoO65I	GGTNACC	1 cut(s)	517
EcoRI	GAATTC	3 cut(s)	1409, 2068, 2110
EcoRII	CCWGG	4 cut(s)	994, 1222, 1839, 2035
EcoRV	GATATC	1 cut(s)	1537
EcoT14I	CCWWGG	2 cut(s)	936, 1104
ErhI	CCWWGG	2 cut(s)	936, 1104
FaeI	CATG	7 cut(s)	1007, 1108, 1292, 1424, 1505, 1956, 1988
FaqI	GGGAC	1 cut(s)	954
FatI	CATG	7 cut(s)	1003, 1104, 1288, 1420, 1501, 1952, 1984
FauI	CCCGC	1 cut(s)	138
FbaI	TGATCA	1 cut(s)	1135
FblI	GTMKAC	1 cut(s)	2052
Fnu4HI	GCNGC	8 cut(s)	33, 449, 798, 801, 1053, 1929, 1932, 2203
FokI	GGATG	1 cut(s)	831
Fsp4HI	GCNGC	8 cut(s)	33, 449, 798, 801, 1053, 1929, 1932, 2203
FspBI	CTAG	7 cut(s)	147, 657, 681, 1331, 1490, 1745, 1911
GlaI	GCGC	1 cut(s)	565
GluI	GCNGC	8 cut(s)	33, 449, 798, 801, 1053, 1929, 1932, 2203
GsuI	CTGGAG	1 cut(s)	1662
HaeII	RGCGCY	1 cut(s)	567
HaeIII	GGCC	7 cut(s)	605, 635, 764, 807, 1172, 1601, 1822
HapII	CCGG	9 cut(s)	86, 515, 761, 911, 1267, 1312, 1758, 2086, 2212
HgaI	GACGC	1 cut(s)	988
HhaI	GCGC	1 cut(s)	566
Hin1I	GRCGYC	2 cut(s)	999, 1762
Hin1II	CATG	7 cut(s)	1007, 1108, 1292, 1424, 1505, 1956, 1988
Hin6I	GCGC	1 cut(s)	564
HinP1I	GCGC	1 cut(s)	564
HincII	GTYRAC	2 cut(s)	501, 1300
HindII	GTYRAC	2 cut(s)	501, 1300
HinfI	GANTC	2 cut(s)	479, 1784
HpaII	CCGG	9 cut(s)	86, 515, 761, 911, 1267, 1312, 1758, 2086, 2212
Hpy166II	GTNNAC	5 cut(s)	172, 501, 1207, 1300, 2053
Hpy188I	TCNGA	8 cut(s)	780, 860, 1063, 1168, 1566, 1588, 1717, 2225
Hpy188III	TCNNGA	6 cut(s)	333, 911, 1532, 1679, 2021, 2135
Hpy8I	GTNNAC	5 cut(s)	172, 501, 1207, 1300, 2053
HpyAV	CCTTC	9 cut(s)	36, 288, 312, 348, 660, 1187, 1225, 1988, 2018
HpyCH4III	ACNGT	7 cut(s)	75, 111, 750, 1352, 1438, 2045, 2119
HpyCH4IV	ACGT	2 cut(s)	1084, 1762
HpyCH4V	TGCA	8 cut(s)	772, 1076, 1241, 1318, 1420, 1481, 1512, 2162
HpyF10VI	GCNNNNNNNGC	6 cut(s)	652, 1276, 1324, 1859, 1928, 2202
HpyF3I	CTNAG	5 cut(s)	1062, 1293, 1365, 1405, 2191
HpySE526I	ACGT	2 cut(s)	1084, 1762
Hsp92I	GRCGYC	2 cut(s)	999, 1762
Hsp92II	CATG	7 cut(s)	1007, 1108, 1292, 1424, 1505, 1956, 1988
HspAI	GCGC	1 cut(s)	564
Kpn2I	TCCGGA	1 cut(s)	910
KpnI	GGTACC	2 cut(s)	1620, 1672
KroI	GCCGGC	2 cut(s)	760, 1266
KroNI	GCCGGC	2 cut(s)	762, 1268
Ksp22I	TGATCA	1 cut(s)	1135
Kzo9I	GATC	5 cut(s)	732, 1135, 1561, 1870, 2014
LmnI	GCTCC	3 cut(s)	571, 1276, 1681
Lsp1109I	GCAGC	7 cut(s)	19, 435, 809, 812, 1915, 1918, 2214
LweI	GCATC	6 cut(s)	952, 1327, 1336, 1553, 1697, 1849
MaeI	CTAG	7 cut(s)	147, 657, 681, 1331, 1490, 1745, 1911
MaeII	ACGT	2 cut(s)	1084, 1762
MalI	GATC	5 cut(s)	734, 1137, 1563, 1872, 2016
MboI	GATC	5 cut(s)	732, 1135, 1561, 1870, 2014
MboII	GAAGA	8 cut(s)	143, 926, 1517, 1856, 1909, 2009, 2111, 2119
MfeI	CAATTG	2 cut(s)	1139, 2056
MflI	RGATCY	2 cut(s)	1870, 2014
MhlI	GDGCHC	1 cut(s)	1350
MlsI	TGGCCA	3 cut(s)	605, 635, 1601
MluCI	AATT	7 cut(s)	861, 1139, 1409, 1888, 2056, 2068, 2110
MluNI	TGGCCA	3 cut(s)	605, 635, 1601
MlyI	GAGTC	1 cut(s)	473
MmeI	TCCRAC	3 cut(s)	241, 494, 811
Mox20I	TGGCCA	3 cut(s)	605, 635, 1601
MroI	TCCGGA	1 cut(s)	910
MroNI	GCCGGC	2 cut(s)	760, 1266
MscI	TGGCCA	3 cut(s)	605, 635, 1601
MseI	TTAA	2 cut(s)	1253, 2184
MslI	CAYNNNNRTG	3 cut(s)	13, 981, 1419
Msp20I	TGGCCA	3 cut(s)	605, 635, 1601
MspA1I	CMGCKG	2 cut(s)	448, 1055
MspI	CCGG	9 cut(s)	86, 515, 761, 911, 1267, 1312, 1758, 2086, 2212
MspR9I	CCNGG	7 cut(s)	996, 1224, 1313, 1841, 2037, 2086, 2212
MunI	CAATTG	2 cut(s)	1139, 2056
MvaI	CCWGG	4 cut(s)	996, 1224, 1841, 2037
MwoI	GCNNNNNNNGC	6 cut(s)	652, 1276, 1324, 1859, 1928, 2202
NaeI	GCCGGC	2 cut(s)	762, 1268
NciI	CCSGG	3 cut(s)	1313, 2086, 2212
NcoI	CCATGG	1 cut(s)	1104
NdeII	GATC	5 cut(s)	732, 1135, 1561, 1870, 2014
NgoMIV	GCCGGC	2 cut(s)	760, 1266
NlaIII	CATG	7 cut(s)	1007, 1108, 1292, 1424, 1505, 1956, 1988
NlaIV	GGNNCC	4 cut(s)	942, 1618, 1670, 1872
NmuCI	GTSAC	7 cut(s)	43, 75, 111, 235, 259, 517, 1702
OliI	CACNNNNGTG	1 cut(s)	13
PaeR7I	CTCGAG	1 cut(s)	1647
PceI	AGGCCT	1 cut(s)	807
PdiI	GCCGGC	2 cut(s)	762, 1268
PfeI	GAWTC	1 cut(s)	1784
PflMI	CCANNNNNTGG	1 cut(s)	950
PkrI	GCNGC	8 cut(s)	34, 450, 799, 802, 1054, 1930, 1933, 2204
PleI	GAGTC	1 cut(s)	473
PpsI	GAGTC	1 cut(s)	473
PsiI	TTATAA	2 cut(s)	1754, 2079
Psp6I	CCWGG	4 cut(s)	994, 1222, 1839, 2035
PspEI	GGTNACC	1 cut(s)	517
PspGI	CCWGG	4 cut(s)	994, 1222, 1839, 2035
PspN4I	GGNNCC	4 cut(s)	942, 1618, 1670, 1872
PspPI	GGNCC	2 cut(s)	214, 941
PspXI	VCTCGAGB	1 cut(s)	1647
PsuI	RGATCY	2 cut(s)	1870, 2014
RsaI	GTAC	8 cut(s)	171, 867, 1218, 1618, 1670, 1685, 1740, 1777
RsaNI	GTAC	8 cut(s)	170, 866, 1217, 1617, 1669, 1684, 1739, 1776
RseI	CAYNNNNRTG	3 cut(s)	13, 981, 1419
SaqAI	TTAA	2 cut(s)	1253, 2184
SatI	GCNGC	8 cut(s)	33, 449, 798, 801, 1053, 1929, 1932, 2203
Sau3AI	GATC	5 cut(s)	732, 1135, 1561, 1870, 2014
Sau96I	GGNCC	2 cut(s)	214, 941
ScaI	AGTACT	1 cut(s)	1218
SchI	GAGTC	1 cut(s)	473
ScrFI	CCNGG	7 cut(s)	996, 1224, 1313, 1841, 2037, 2086, 2212
SduI	GDGCHC	1 cut(s)	1350
SfaNI	GCATC	6 cut(s)	952, 1327, 1336, 1553, 1697, 1849
Sfr274I	CTCGAG	1 cut(s)	1647
SinI	GGWCC	2 cut(s)	214, 941
SlaI	CTCGAG	1 cut(s)	1647
SmiMI	CAYNNNNRTG	3 cut(s)	13, 981, 1419
SmlI	CTYRAG	4 cut(s)	1647, 1789, 1833, 2197
SmoI	CTYRAG	4 cut(s)	1647, 1789, 1833, 2197
SpeI	ACTAGT	1 cut(s)	146
Sse9I	AATT	7 cut(s)	861, 1139, 1409, 1888, 2056, 2068, 2110
SseBI	AGGCCT	1 cut(s)	807
SspI	AATATT	1 cut(s)	1552
SspMI	CTAG	7 cut(s)	147, 657, 681, 1331, 1490, 1745, 1911
StuI	AGGCCT	1 cut(s)	807
StyD4I	CCNGG	7 cut(s)	994, 1222, 1311, 1839, 2035, 2084, 2210
StyI	CCWWGG	2 cut(s)	936, 1104
TaaI	ACNGT	7 cut(s)	75, 111, 750, 1352, 1438, 2045, 2119
TaiI	ACGT	2 cut(s)	1087, 1765
TaqI	TCGA	3 cut(s)	1648, 1942, 1960
TasI	AATT	7 cut(s)	861, 1139, 1409, 1888, 2056, 2068, 2110
TatI	WGTACW	5 cut(s)	169, 865, 1216, 1738, 1775
TauI	GCSGC	1 cut(s)	1055
TfiI	GAWTC	1 cut(s)	1784
Tru1I	TTAA	2 cut(s)	1253, 2184
Tru9I	TTAA	2 cut(s)	1253, 2184
TscAI	CASTG	7 cut(s)	606, 1248, 1355, 1817, 2050, 2164, 2176
TseFI	GTSAC	7 cut(s)	43, 75, 111, 235, 259, 517, 1702
TseI	GCWGC	7 cut(s)	32, 448, 797, 800, 1928, 1931, 2202
Tsp45I	GTSAC	7 cut(s)	43, 75, 111, 235, 259, 517, 1702
TspDTI	ATGAA	5 cut(s)	1277, 1326, 1517, 1518, 1716
TspRI	CASTG	7 cut(s)	606, 1248, 1355, 1817, 2050, 2164, 2176
Van91I	CCANNNNNTGG	1 cut(s)	950
VpaK11BI	GGWCC	2 cut(s)	214, 941
XapI	RAATTY	3 cut(s)	1409, 2068, 2110
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1651
XhoI	CTCGAG	1 cut(s)	1647
XmiI	GTMKAC	1 cut(s)	2052
XspI	CTAG	7 cut(s)	147, 657, 681, 1331, 1490, 1745, 1911
ZraI	GACGTC	1 cut(s)	1763
ZrmI	AGTACT	1 cut(s)	1218

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.