RchiOBHm_Chr6g0307421

protein At1g47420, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011296
Physical Location & Seq
Forward (+)
65826363 .. 65828171
1809 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27638

Sequence Viewer

Length: 729 bp
ATGGAGAAGATGATAGCTCTCAGATCGCTCTGCCGTTCACTCTCTTCCAGATCTTACCGAACAGCCGCCGCCACCCAGCAGCTGCCACGTCACCAACATCACCACCACTCCGCCGGCGCTGCTCGAACCCTCTTCAGTTTCGCTTCCCCAAACTCCAAGAACCAGTCTTCCGATGAACATAGGTCTCCTCTTTCAACGAGTTTGGGGAGTAGGCGGTTTTCCAGCAACGATGTGGGTCATTTTCCTGAGATTAAAGACCCTGTGCTGCTTAATGCTTTTAAGGACTTGATGGCTGCAAGTTGGGATGAGCTCCCTAAGCTTGTGGTCCGTGAAGTTAAGGCAGCATTGTCTAAGAACACTGATGATCAGACTGGCAAAGAGCATCTCATGAATGTGTTGCGTGCGGCCGAAGCTGTGGAGGAGTTTGGAGGGATGGTTATGCAGTTGAAGATGGATATGGATGACAGTGTCGGTCTGAGTGGAGAGGATGTGAAGCCGTTGTCAGATGAGTATGTGAATGCTCTGAAGACGATTTACAGTCGCTACGCTGCCTATTTGGATTCATTTGGGCCTGACGAGACCTATTTGAGGAAGAAGGTGGAGACTGAGCTGGGAACCAGGCTGATATACTTGAAGATGAGGTGTAGTGGCATTGGTTCTGAGTGGGGAAAGGTCAGTGTTCTTGGAACTTCTGGACTGTCAGGGTCATATGTTGAGCAAAGAGCTTAG

Protein Analysis

242

Amino Acids

26.84

Weight (kDa)

7.8

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SDH5_plant PF14290 1 - 242 5.4e-108 Succinate dehydrogenase subunit 5, mitochondrial
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011296)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47420
fragaria_vesca FvH4_2g34650 FvH4_2g34650 FvH4_2g34710
malus_domestica MD04G1229600.v1.1 MD08G1103900.v1.1 MD15G1085100.v1.1
prunus_persica Prupe.1G439200_v2.0.a1 Prupe.1G439200_v2.0.a1
pyrus_communis pycom08g08520 pycom15g08040
rosa_chinensis RchiOBHm_Chr6g0307421
rosa_laevigata RLG00000010715
rosa_multiflora Rmu_sc0000031.1_g000016
rosa_roxburghii Rroxscaffold_7G00161010
rosa_rugosa Rorug06G0358500 Rorug06G0358600
rosa_samantha Rh6CG484200 Rh6DG470900
rosa_wichuraiana Rw6G040890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 66, 69, 111, 214, 404
AcoI YGGCCR 1 cut(s) 405
AcuI CTGAAG 2 cut(s) 118, 545
AfiI CCNNNNNNNGG 1 cut(s) 588
AgsI TTSAA 3 cut(s) 195, 448, 634
AjiI CACGTC 1 cut(s) 89
AjnI CCWGG 1 cut(s) 617
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 7 cut(s) 17, 82, 310, 319, 413, 610, 725
AluI AGCT 7 cut(s) 17, 82, 310, 319, 413, 610, 725
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 3 cut(s) 189, 572, 596
AlwNI CAGNNNCTG 1 cut(s) 82
AoxI GGCC 2 cut(s) 405, 569
ApeKI GCWGC 7 cut(s) 79, 82, 119, 265, 293, 341, 548
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 325, 569
AsuHPI GGTGA 2 cut(s) 83, 92
AvaII GGWCC 1 cut(s) 325
BanII GRGCYC 1 cut(s) 312
BarI GAAGNNNNNNTAC 2 cut(s) 518, 550
BbsI GAAGAC 2 cut(s) 159, 533
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 7 cut(s) 69, 91, 106, 252, 280, 353, 535
BccI CCATC 3 cut(s) 283, 427, 445
BceAI ACGGC 2 cut(s) 18, 481
BciT130I CCWGG 1 cut(s) 619
BclI TGATCA 1 cut(s) 364
BcoDI GTCTC 3 cut(s) 189, 572, 596
BfoI RGCGCY 1 cut(s) 120
BglII AGATCT 1 cut(s) 50
Bme1390I CCNGG 1 cut(s) 619
Bme18I GGWCC 1 cut(s) 325
BmgBI CACGTC 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 325, 569
BmiI GGNNCC 1 cut(s) 616
BmrFI CCNGG 1 cut(s) 619
BmsI GCATC 1 cut(s) 391
BpiI GAAGAC 2 cut(s) 159, 533
Bpu10I CCTNAGC 1 cut(s) 315
BsaI GGTCTC 2 cut(s) 189, 572
BsaXI ACNNNNNCTCC 6 cut(s) 92, 122, 420, 450, 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 588
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 2 cut(s) 163, 376
BseBI CCWGG 1 cut(s) 619
BseGI GGATG 4 cut(s) 310, 438, 466, 493
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMII CTCAG 5 cut(s) 34, 237, 467, 597, 651
BseNI ACTGG 2 cut(s) 163, 376
BseRI GAGGAG 2 cut(s) 177, 434
BseX3I CGGCCG 1 cut(s) 405
BseXI GCAGC 7 cut(s) 69, 91, 106, 252, 280, 353, 535
BseYI CCCAGC 2 cut(s) 75, 610
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 2 cut(s) 407, 571
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 312
BsiSI CCGG 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 588
BsmAI GTCTC 3 cut(s) 189, 572, 596
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 2 cut(s) 407, 571
Bso31I GGTCTC 2 cut(s) 189, 572
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 3 cut(s) 23, 50, 364
BspACI CCGC 5 cut(s) 66, 69, 111, 214, 404
BspANI GGCC 2 cut(s) 407, 571
BspCNI CTCAG 5 cut(s) 33, 238, 468, 598, 652
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 616
BspTNI GGTCTC 2 cut(s) 189, 572
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 2 cut(s) 163, 376
BssAI RCCGGY 1 cut(s) 113
BssMI GATC 3 cut(s) 23, 50, 364
Bst2UI CCWGG 1 cut(s) 619
Bst4CI ACNGT 3 cut(s) 467, 539, 699
Bst6I CTCTTC 2 cut(s) 49, 137
BstC8I GCNNGC 2 cut(s) 115, 402
BstDEI CTNAG 8 cut(s) 20, 246, 315, 351, 476, 606, 660, 726
BstENI CCTNNNNNAGG 1 cut(s) 586
BstF5I GGATG 4 cut(s) 310, 438, 466, 493
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 3 cut(s) 26, 53, 367
BstMAI GTCTC 3 cut(s) 189, 572, 596
BstMBI GATC 3 cut(s) 23, 50, 364
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 3 cut(s) 119, 316, 410
BstNI CCWGG 1 cut(s) 619
BstSCI CCNGG 1 cut(s) 617
BstV1I GCAGC 7 cut(s) 69, 91, 106, 252, 280, 353, 535
BstV2I GAAGAC 2 cut(s) 159, 533
BstX2I RGATCY 1 cut(s) 50
BstYI RGATCY 1 cut(s) 50
BstZI CGGCCG 1 cut(s) 405
BsuRI GGCC 2 cut(s) 407, 571
BtrI CACGTC 1 cut(s) 89
BtsCI GGATG 4 cut(s) 310, 438, 466, 493
BtsIMutI CAGTG 3 cut(s) 357, 472, 682
Cac8I GCNNGC 2 cut(s) 115, 402
CaiI CAGNNNCTG 1 cut(s) 82
CciI TCATGA 1 cut(s) 387
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 325, 569
CviAII CATG 1 cut(s) 388
DdeI CTNAG 8 cut(s) 20, 246, 315, 351, 476, 606, 660, 726
DpnI GATC 3 cut(s) 25, 52, 366
DpnII GATC 3 cut(s) 23, 50, 364
EaeI YGGCCR 1 cut(s) 405
EagI CGGCCG 1 cut(s) 405
Eam1104I CTCTTC 2 cut(s) 49, 137
EarI CTCTTC 2 cut(s) 49, 137
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 1 cut(s) 310
EclXI CGGCCG 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 312
Eco31I GGTCTC 2 cut(s) 189, 572
Eco47I GGWCC 1 cut(s) 325
Eco52I CGGCCG 1 cut(s) 405
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 2 cut(s) 118, 545
EcoICRI GAGCTC 1 cut(s) 310
EcoNI CCTNNNNNAGG 1 cut(s) 586
EcoRII CCWGG 1 cut(s) 617
EcoT38I GRGCYC 1 cut(s) 312
FaeI CATG 1 cut(s) 391
FaiI YATR 8 cut(s) 180, 389, 440, 458, 513, 628, 709, 711
FatI CATG 1 cut(s) 387
FauNDI CATATG 1 cut(s) 709
FbaI TGATCA 1 cut(s) 364
FokI GGATG 4 cut(s) 317, 445, 473, 500
FriOI GRGCYC 1 cut(s) 312
GlaI GCGC 1 cut(s) 118
GsaI CCCAGC 2 cut(s) 79, 614
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 2 cut(s) 407, 571
HapII CCGG 1 cut(s) 114
HhaI GCGC 1 cut(s) 119
Hin1II CATG 1 cut(s) 391
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 1 cut(s) 560
HpaII CCGG 1 cut(s) 114
HphI GGTGA 2 cut(s) 83, 92
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 7 cut(s) 23, 172, 369, 477, 505, 525, 661
Hpy188III TCNNGA 4 cut(s) 48, 245, 388, 693
Hpy8I GTNNAC 1 cut(s) 38
HpyAV CCTTC 1 cut(s) 589
HpyCH4III ACNGT 3 cut(s) 467, 539, 699
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 2 cut(s) 296, 442
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 316, 410
HpyF3I CTNAG 8 cut(s) 20, 246, 315, 351, 476, 606, 660, 726
HpySE526I ACGT 1 cut(s) 88
Hsp92II CATG 1 cut(s) 391
HspAI GCGC 1 cut(s) 117
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 3 cut(s) 23, 50, 364
LmnI GCTCC 1 cut(s) 315
Lsp1109I GCAGC 7 cut(s) 69, 91, 106, 252, 280, 353, 535
LweI GCATC 1 cut(s) 391
MaeII ACGT 1 cut(s) 88
MaeIII GTNAC 1 cut(s) 89
MalI GATC 3 cut(s) 25, 52, 366
MboI GATC 3 cut(s) 23, 50, 364
MboII GAAGA 8 cut(s) 19, 36, 124, 159, 460, 538, 604, 646
MflI RGATCY 1 cut(s) 50
MhlI GDGCHC 1 cut(s) 312
MnlI CCTC 7 cut(s) 140, 198, 412, 422, 478, 582, 633
MreI CGCCGGCG 1 cut(s) 113
MroNI GCCGGC 1 cut(s) 113
MseI TTAA 4 cut(s) 252, 270, 279, 336
MslI CAYNNNNRTG 1 cut(s) 392
MspA1I CMGCKG 1 cut(s) 82
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 1 cut(s) 619
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 1 cut(s) 619
MwoI GCNNNNNNNGC 3 cut(s) 119, 316, 410
NaeI GCCGGC 1 cut(s) 115
NdeI CATATG 1 cut(s) 709
NdeII GATC 3 cut(s) 23, 50, 364
NgoMIV GCCGGC 1 cut(s) 113
NlaIII CATG 1 cut(s) 391
NlaIV GGNNCC 1 cut(s) 616
NmuCI GTSAC 1 cut(s) 89
PagI TCATGA 1 cut(s) 387
PctI GAATGC 1 cut(s) 523
PdiI GCCGGC 1 cut(s) 115
PfeI GAWTC 1 cut(s) 560
PflFI GACNNNGTC 1 cut(s) 467
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 617
PspFI CCCAGC 2 cut(s) 75, 610
PspGI CCWGG 1 cut(s) 617
PspN4I GGNNCC 1 cut(s) 616
PspPI GGNCC 2 cut(s) 325, 569
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 50
PsyI GACNNNGTC 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 82
RseI CAYNNNNRTG 1 cut(s) 392
SacI GAGCTC 1 cut(s) 312
SaqAI TTAA 4 cut(s) 252, 270, 279, 336
Sau3AI GATC 3 cut(s) 23, 50, 364
Sau96I GGNCC 2 cut(s) 325, 569
ScrFI CCNGG 1 cut(s) 619
SduI GDGCHC 1 cut(s) 312
SfaNI GCATC 1 cut(s) 391
SgrAI CRCCGGYG 1 cut(s) 113
SinI GGWCC 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 392
SsiI CCGC 5 cut(s) 66, 69, 111, 214, 404
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 1 cut(s) 617
TaaI ACNGT 3 cut(s) 467, 539, 699
TaiI ACGT 1 cut(s) 91
TaqI TCGA 1 cut(s) 124
TaqII GACCGA 1 cut(s) 461
TauI GCSGC 3 cut(s) 68, 71, 407
TfiI GAWTC 1 cut(s) 560
Tru1I TTAA 4 cut(s) 252, 270, 279, 336
Tru9I TTAA 4 cut(s) 252, 270, 279, 336
TscAI CASTG 3 cut(s) 364, 472, 682
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 7 cut(s) 79, 82, 119, 265, 293, 341, 548
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 3 cut(s) 189, 404, 552
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 3 cut(s) 364, 472, 682
Tth111I GACNNNGTC 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 325
XagI CCTNNNNNAGG 1 cut(s) 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.