RchiOBHm_Chr7g0241511

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013689
Physical Location & Seq
Reverse (-)
67592177 .. 67600280
8104 bp
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UTR
Exon/CDS
Intron
PRQ21641

Sequence Viewer

Length: 237 bp
ATGGAGAAGCCGTTACAAGTAGCAAATGCTAGGGACTATATGAGGCGATTCTGTTCCTCTCTTGGCTTGAACAATCAAGCAATCAAAGCAGCACAACAAGCTGTGCACAAGTCGCAAGAATTTGATATAAGGAGGAGCCCTGTATCGATAGCCGCAGCAGTGATTTGCATCATCACTCAGCTTTCAGATGTAGTAACAAGAAGCCTCTCAAAGATATCGCAGACGTTACAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

78

Amino Acids

8.67

Weight (kDa)

9.59

Isoelectric Point (pI)

69.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIB PF00382 6 - 77 1.1e-08 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 153
AcsI RAATTY 1 cut(s) 119
AgsI TTSAA 1 cut(s) 70
AluBI AGCT 2 cut(s) 101, 181
AluI AGCT 2 cut(s) 101, 181
Alw21I GWGCWC 1 cut(s) 108
Alw44I GTGCAC 1 cut(s) 104
ApaLI GTGCAC 1 cut(s) 104
ApeKI GCWGC 2 cut(s) 89, 155
ApoI RAATTY 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 101, 167
BfaI CTAG 1 cut(s) 30
BisI GCNGC 3 cut(s) 90, 153, 156
BlsI GCNGC 3 cut(s) 91, 154, 157
BmiI GGNNCC 1 cut(s) 137
BmsI GCATC 1 cut(s) 177
Bsa29I ATCGAT 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 167
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 167
BseCI ATCGAT 1 cut(s) 146
BseJI GATNNNNATC 1 cut(s) 167
BseMII CTCAG 1 cut(s) 191
BseRI GAGGAG 1 cut(s) 148
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 2 cut(s) 101, 167
BshVI ATCGAT 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 108
BslFI GGGAC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 108, 140
BspACI CCGC 1 cut(s) 153
BspCNI CTCAG 1 cut(s) 190
BspDI ATCGAT 1 cut(s) 146
BspLI GGNNCC 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 177
BstMWI GCNNNNNNNGC 3 cut(s) 86, 98, 112
BstSLI GKGCMC 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 101, 167
Bsu15I ATCGAT 1 cut(s) 146
BsuTUI ATCGAT 1 cut(s) 146
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 165
ClaI ATCGAT 1 cut(s) 146
CviJI RGCY 7 cut(s) 10, 66, 101, 138, 152, 181, 204
CviKI_1 RGCY 7 cut(s) 10, 66, 101, 138, 152, 181, 204
DdeI CTNAG 1 cut(s) 177
Eco24I GRGCYC 1 cut(s) 140
Eco32I GATATC 1 cut(s) 216
EcoRV GATATC 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 140
FaiI YATR 3 cut(s) 39, 41, 128
FaqI GGGAC 1 cut(s) 47
Fnu4HI GCNGC 3 cut(s) 90, 153, 156
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 3 cut(s) 90, 153, 156
FspBI CTAG 1 cut(s) 30
GluI GCNGC 3 cut(s) 90, 153, 156
HinfI GANTC 1 cut(s) 48
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 187
Hpy8I GTNNAC 1 cut(s) 106
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 2 cut(s) 106, 168
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 98, 112
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 224
LmnI GCTCC 1 cut(s) 135
LpnPI CCDG 2 cut(s) 153, 215
Lsp1109I GCAGC 2 cut(s) 101, 167
LweI GCATC 1 cut(s) 177
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 3 cut(s) 12, 193, 225
MhlI GDGCHC 2 cut(s) 108, 140
MluCI AATT 1 cut(s) 119
MnlI CCTC 4 cut(s) 36, 67, 126, 215
MwoI GCNNNNNNNGC 3 cut(s) 86, 98, 112
NlaIV GGNNCC 1 cut(s) 137
PfeI GAWTC 1 cut(s) 48
PkrI GCNGC 3 cut(s) 91, 154, 157
PspN4I GGNNCC 1 cut(s) 137
SatI GCNGC 3 cut(s) 90, 153, 156
SduI GDGCHC 2 cut(s) 108, 140
SetI ASST 3 cut(s) 103, 183, 227
SfaNI GCATC 1 cut(s) 177
Sse9I AATT 1 cut(s) 119
SsiI CCGC 1 cut(s) 153
SspMI CTAG 1 cut(s) 30
TaiI ACGT 1 cut(s) 227
TaqI TCGA 1 cut(s) 146
TasI AATT 1 cut(s) 119
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 1 cut(s) 48
TscAI CASTG 1 cut(s) 165
TseI GCWGC 2 cut(s) 89, 155
TspRI CASTG 1 cut(s) 165
VneI GTGCAC 1 cut(s) 104
XapI RAATTY 1 cut(s) 119
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.