Rh7AG500900

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0013689
Physical Location & Seq
Forward (+)
70038980 .. 70039570
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG500900.1

Sequence Viewer

Length: 591 bp
ATGGCCTTCAAAACCATCGAGACCATGTGTGATAGGTTGGGCCTTGTGTCAACCATCAAGAACCGGGCAAATGAGATGTACAAGACTCTTGAAGATCACAAGTGTAGCAAGGGAAGAAACCAAGATGCATTATTGGCTGCTTGCCTATACATTGCTTGTAAACAAGAAGACAAGCCCAGAACTCTCAAGGAAATCTTCTCCGTCGCGAATGGAGCCTCAAAGAAAGAGATTGGTAGAGCAAAGGAATACATAATCAAGCAACTGGGGCTAATGGAGAAGCCATTTCAAGTAGCAAATGCTGGGGACTATATGAGGCGATTCTGTTCCTCTCTTGGCTTGAACAATCAAGCAATCAAAGCAGCTCAACAAGCTGTGCACAAGTCGCAAGAAATTGATATAAGGAGGAGCCCTGTATCGATAGCCGCAGCAGTGATTTACATCATCACTCAGCTTTCAGATGATAGTAACAAGAGGCCTCTCAAAGATATCGCAGACGTTACAGGAGTAGCACCAGCAACTATCAAAAATTCATACAAAGACCTTAGTCCTCATGTATCTGAAATAATACCCAACTGGTATCATCTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

196

Amino Acids

21.92

Weight (kDa)

9.31

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIB PF00382 2 - 79 1e-19 Transcription factor TFIIB repeat
TFIIB PF00382 97 - 182 1e-12 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 206
AciI CCGC 1 cut(s) 423
AcsI RAATTY 1 cut(s) 526
AfaI GTAC 1 cut(s) 80
AgsI TTSAA 4 cut(s) 10, 92, 287, 340
AluBI AGCT 3 cut(s) 362, 371, 451
AluI AGCT 3 cut(s) 362, 371, 451
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 14
Alw44I GTGCAC 1 cut(s) 374
AoxI GGCC 3 cut(s) 3, 40, 473
ApaLI GTGCAC 1 cut(s) 374
ApeKI GCWGC 3 cut(s) 137, 359, 425
ApoI RAATTY 1 cut(s) 526
AspS9I GGNCC 1 cut(s) 40
AsuC2I CCSGG 1 cut(s) 65
BaeGI GKGCMC 1 cut(s) 378
BanII GRGCYC 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 3 cut(s) 124, 371, 437
BccI CCATC 2 cut(s) 23, 62
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 14
BisI GCNGC 4 cut(s) 138, 360, 423, 426
BlsI GCNGC 4 cut(s) 139, 361, 424, 427
Bme1390I CCNGG 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 40
BmiI GGNNCC 2 cut(s) 214, 407
BmrFI CCNGG 1 cut(s) 65
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 1 cut(s) 115
BmuI ACTGGG 1 cut(s) 272
BoxI GACNNNNGTC 1 cut(s) 543
BpiI GAAGAC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 416
BsaBI GATNNNNATC 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 14
Bse1I ACTGG 2 cut(s) 267, 578
Bse3DI GCAATG 1 cut(s) 150
Bse8I GATNNNNATC 1 cut(s) 437
BseCI ATCGAT 1 cut(s) 416
BseJI GATNNNNATC 1 cut(s) 437
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 2 cut(s) 267, 578
BseRI GAGGAG 1 cut(s) 418
BseSI GKGCMC 1 cut(s) 378
BseXI GCAGC 3 cut(s) 124, 371, 437
BseYI CCCAGC 1 cut(s) 299
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 3 cut(s) 5, 42, 475
BshVI ATCGAT 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 1 cut(s) 64
BslFI GGGAC 1 cut(s) 317
BsmAI GTCTC 1 cut(s) 14
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 3 cut(s) 5, 42, 475
Bso31I GGTCTC 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 378, 410
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 1 cut(s) 94
Bsp68I TCGCGA 1 cut(s) 206
BspACI CCGC 1 cut(s) 423
BspANI GGCC 3 cut(s) 5, 42, 475
BspCNI CTCAG 1 cut(s) 460
BspDI ATCGAT 1 cut(s) 416
BspFNI CGCG 1 cut(s) 206
BspLI GGNNCC 2 cut(s) 214, 407
BspTNI GGTCTC 1 cut(s) 14
BsrDI GCAATG 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 2 cut(s) 267, 578
BssMI GATC 1 cut(s) 94
BstAUI TGTACA 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 2 cut(s) 447, 542
BstFNI CGCG 1 cut(s) 206
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 6 cut(s) 134, 212, 265, 356, 368, 382
BstPAI GACNNNNGTC 1 cut(s) 543
BstSCI CCNGG 1 cut(s) 63
BstSLI GKGCMC 1 cut(s) 378
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 3 cut(s) 124, 371, 437
BstV2I GAAGAC 1 cut(s) 174
Bsu15I ATCGAT 1 cut(s) 416
BsuRI GGCC 3 cut(s) 5, 42, 475
BsuTUI ATCGAT 1 cut(s) 416
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 435
BtuMI TCGCGA 1 cut(s) 206
Cac8I GCNNGC 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 40
ClaI ATCGAT 1 cut(s) 416
Csp6I GTAC 1 cut(s) 79
CviAII CATG 2 cut(s) 25, 551
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 2 cut(s) 447, 542
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
Eco147I AGGCCT 1 cut(s) 475
Eco24I GRGCYC 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 14
Eco32I GATATC 1 cut(s) 487
EcoRV GATATC 1 cut(s) 487
EcoT22I ATGCAT 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 410
FaeI CATG 2 cut(s) 28, 554
FaiI YATR 8 cut(s) 26, 148, 251, 309, 311, 398, 532, 552
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 1 cut(s) 317
FatI CATG 2 cut(s) 24, 550
Fnu4HI GCNGC 4 cut(s) 138, 360, 423, 426
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 4 cut(s) 138, 360, 423, 426
GluI GCNGC 4 cut(s) 138, 360, 423, 426
GsaI CCCAGC 1 cut(s) 303
HaeIII GGCC 3 cut(s) 5, 42, 475
HapII CCGG 1 cut(s) 64
Hin1II CATG 2 cut(s) 28, 554
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 2 cut(s) 85, 318
HpaII CCGG 1 cut(s) 64
Hpy166II GTNNAC 3 cut(s) 51, 161, 376
Hpy188I TCNGA 3 cut(s) 457, 559, 585
Hpy188III TCNNGA 4 cut(s) 19, 58, 89, 205
Hpy8I GTNNAC 3 cut(s) 51, 161, 376
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 16
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 2 cut(s) 128, 376
HpyF10VI GCNNNNNNNGC 6 cut(s) 134, 212, 265, 356, 368, 382
HpyF3I CTNAG 2 cut(s) 447, 542
HpySE526I ACGT 1 cut(s) 495
Hsp92II CATG 2 cut(s) 28, 554
Kzo9I GATC 1 cut(s) 94
LmnI GCTCC 2 cut(s) 212, 405
LpnPI CCDG 8 cut(s) 77, 190, 248, 285, 423, 486, 525, 559
Lsp1109I GCAGC 3 cut(s) 124, 371, 437
LweI GCATC 1 cut(s) 115
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 2 cut(s) 464, 496
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MboII GAAGA 4 cut(s) 104, 126, 179, 187
MhlI GDGCHC 2 cut(s) 378, 410
MluCI AATT 2 cut(s) 390, 526
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 7 cut(s) 226, 306, 337, 396, 465, 486, 558
Mph1103I ATGCAT 1 cut(s) 130
MspI CCGG 1 cut(s) 64
MspR9I CCNGG 1 cut(s) 65
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 6 cut(s) 134, 212, 265, 356, 368, 382
NciI CCSGG 1 cut(s) 65
NdeII GATC 1 cut(s) 94
NlaIII CATG 2 cut(s) 28, 554
NlaIV GGNNCC 2 cut(s) 214, 407
NruI TCGCGA 1 cut(s) 206
NsiI ATGCAT 1 cut(s) 130
PceI AGGCCT 1 cut(s) 475
PfeI GAWTC 1 cut(s) 318
PkrI GCNGC 4 cut(s) 139, 361, 424, 427
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
PshAI GACNNNNGTC 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 214, 407
PspPI GGNCC 1 cut(s) 40
RruI TCGCGA 1 cut(s) 206
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SatI GCNGC 4 cut(s) 138, 360, 423, 426
Sau3AI GATC 1 cut(s) 94
Sau96I GGNCC 1 cut(s) 40
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 2 cut(s) 378, 410
SetI ASST 6 cut(s) 38, 364, 373, 453, 498, 543
SfaNI GCATC 1 cut(s) 115
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 2 cut(s) 390, 526
SseBI AGGCCT 1 cut(s) 475
SsiI CCGC 1 cut(s) 423
StuI AGGCCT 1 cut(s) 475
StyD4I CCNGG 1 cut(s) 63
TaiI ACGT 1 cut(s) 498
TaqI TCGA 2 cut(s) 18, 416
TasI AATT 2 cut(s) 390, 526
TatI WGTACW 1 cut(s) 78
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 1 cut(s) 318
TscAI CASTG 1 cut(s) 435
TseI GCWGC 3 cut(s) 137, 359, 425
TspDTI ATGAA 1 cut(s) 519
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 435
VneI GTGCAC 1 cut(s) 374
XapI RAATTY 1 cut(s) 526
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.