Rorug03G0267400

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013689
Physical Location & Seq
Forward (+)
26226412 .. 26226975
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0267400.1

Sequence Viewer

Length: 564 bp
ATGACAGTACCATATGGCTCTACTACTGGTTATGTGTCTCAAGAGGTGACAAATTTAGGTGCAAGGTCGCTTGCTCATCATCAACAAAACCTGTCATTGCTTCCAAGCTTTTCGCAGCTTCCAGTTATTAACAGTGTTACCTCTGCTATTTCCAGCCTTGCTGGAGGAGACAACAACAATGGAACGATCACTACACCAAATGATGGTTCAAATGGATCTGAAAAGGTGATAAATTTGCATAAAAACTTGCATGCAGATCATCAGCAAGACCTTCCATCACTTCAAAACTCTTCGAAGCCCTCTGTTATTAACAGCGTTGGGTCTGCTATTGCCAGTCTTGCCGGCAGTCCAGTGTTTCCTGATTTCCAACAGGTTCCTCGTGGTTTTGGAGCTCAACATGGGACAAATATAGCTCCCATAAATGGAATCCAATCACGTCAGTCCCCTCATGGAATTGGACCTCATCAATCTCGGCCAAGTTTGAACAATGGCTGCCTCCCTCCATTACGAACTTTTGTAGGTTTTCTAGTGCTTTTAGGATTTGGTAAGGTCAGTTCCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

187

Amino Acids

19.47

Weight (kDa)

8.98

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 203
AclWI GGATC 1 cut(s) 223
AcoI YGGCCR 1 cut(s) 473
AcsI RAATTY 2 cut(s) 52, 232
AfaI GTAC 1 cut(s) 9
AfiI CCNNNNNNNGG 2 cut(s) 203, 422
AgsI TTSAA 3 cut(s) 210, 284, 484
AjiI CACGTC 1 cut(s) 437
AjuI GAANNNNNNNTTGG 2 cut(s) 190, 222
AluBI AGCT 4 cut(s) 108, 118, 392, 413
AluI AGCT 4 cut(s) 108, 118, 392, 413
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 2 cut(s) 42, 162
AlwI GGATC 1 cut(s) 223
AoxI GGCC 1 cut(s) 473
ApeKI GCWGC 2 cut(s) 115, 492
ApoI RAATTY 2 cut(s) 52, 232
AspS9I GGNCC 1 cut(s) 458
AsuHPI GGTGA 2 cut(s) 58, 238
AsuII TTCGAA 1 cut(s) 293
AvaII GGWCC 1 cut(s) 458
BanII GRGCYC 1 cut(s) 394
BauI CACGAG 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 2 cut(s) 127, 479
BccI CCATC 2 cut(s) 197, 283
BcoDI GTCTC 2 cut(s) 42, 162
BfaI CTAG 1 cut(s) 527
BisI GCNGC 2 cut(s) 116, 493
BlsI GCNGC 2 cut(s) 117, 494
Bme18I GGWCC 1 cut(s) 458
BmgBI CACGTC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 1 cut(s) 375
BpmI CTGGAG 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 24
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 422
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 4 cut(s) 31, 122, 333, 350
Bse3DI GCAATG 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 203, 422
BseMI GCAATG 1 cut(s) 95
BseNI ACTGG 4 cut(s) 31, 122, 333, 350
BseRI GAGGAG 1 cut(s) 180
BseXI GCAGC 2 cut(s) 127, 479
BshFI GGCC 1 cut(s) 475
BsiHKAI GWGCWC 1 cut(s) 394
BsiSI CCGG 1 cut(s) 342
BslFI GGGAC 2 cut(s) 415, 427
BslI CCNNNNNNNGG 2 cut(s) 203, 422
BsmAI GTCTC 2 cut(s) 42, 162
BsmFI GGGAC 2 cut(s) 415, 427
BsnI GGCC 1 cut(s) 475
Bsp119I TTCGAA 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 3 cut(s) 186, 215, 256
BspANI GGCC 1 cut(s) 475
BspLI GGNNCC 1 cut(s) 375
BspPI GGATC 1 cut(s) 223
BspT104I TTCGAA 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 95
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 4 cut(s) 31, 122, 333, 350
BssAI RCCGGY 1 cut(s) 341
BssMI GATC 3 cut(s) 186, 215, 256
BssSI CACGAG 1 cut(s) 378
Bst2BI CACGAG 1 cut(s) 378
Bst4CI ACNGT 2 cut(s) 7, 134
Bst6I CTCTTC 1 cut(s) 295
BstBI TTCGAA 1 cut(s) 293
BstC8I GCNNGC 3 cut(s) 72, 252, 343
BstKTI GATC 3 cut(s) 189, 218, 259
BstMAI GTCTC 2 cut(s) 42, 162
BstMBI GATC 3 cut(s) 186, 215, 256
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 254
BstV1I GCAGC 2 cut(s) 127, 479
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuRI GGCC 1 cut(s) 475
BtrI CACGTC 1 cut(s) 437
BtsIMutI CAGTG 2 cut(s) 139, 357
Cac8I GCNNGC 3 cut(s) 72, 252, 343
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 458
Csp6I GTAC 1 cut(s) 8
CviAII CATG 3 cut(s) 251, 398, 449
CviJI RGCY 9 cut(s) 18, 108, 118, 156, 298, 392, 413, 475, 492
CviKI_1 RGCY 9 cut(s) 18, 108, 118, 156, 298, 392, 413, 475, 492
CviQI GTAC 1 cut(s) 8
DpnI GATC 3 cut(s) 188, 217, 258
DpnII GATC 3 cut(s) 186, 215, 256
EaeI YGGCCR 1 cut(s) 473
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
Ecl136II GAGCTC 1 cut(s) 392
Eco24I GRGCYC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 458
Eco53kI GAGCTC 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 392
EcoT38I GRGCYC 1 cut(s) 394
FaeI CATG 3 cut(s) 254, 401, 452
FaiI YATR 9 cut(s) 13, 15, 33, 240, 252, 399, 410, 419, 450
FaqI GGGAC 2 cut(s) 415, 427
FatI CATG 3 cut(s) 250, 397, 448
FauNDI CATATG 1 cut(s) 13
Fnu4HI GCNGC 2 cut(s) 116, 493
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 116, 493
FspBI CTAG 1 cut(s) 527
GluI GCNGC 2 cut(s) 116, 493
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 1 cut(s) 475
HapII CCGG 1 cut(s) 342
Hin1II CATG 3 cut(s) 254, 401, 452
HindIII AAGCTT 1 cut(s) 106
HinfI GANTC 1 cut(s) 426
HpaII CCGG 1 cut(s) 342
HphI GGTGA 2 cut(s) 58, 238
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 2 cut(s) 41, 359
HpyAV CCTTC 1 cut(s) 281
HpyCH4III ACNGT 2 cut(s) 7, 134
HpyCH4IV ACGT 1 cut(s) 436
HpyCH4V TGCA 4 cut(s) 62, 238, 250, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpySE526I ACGT 1 cut(s) 436
Hsp92II CATG 3 cut(s) 254, 401, 452
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
Kzo9I GATC 3 cut(s) 186, 215, 256
LmnI GCTCC 2 cut(s) 389, 418
Lsp1109I GCAGC 2 cut(s) 127, 479
MaeI CTAG 1 cut(s) 527
MaeII ACGT 1 cut(s) 436
MaeIII GTNAC 2 cut(s) 46, 136
MalI GATC 3 cut(s) 188, 217, 258
MboI GATC 3 cut(s) 186, 215, 256
MboII GAAGA 1 cut(s) 282
MflI RGATCY 1 cut(s) 215
MhlI GDGCHC 1 cut(s) 394
MluCI AATT 3 cut(s) 52, 232, 453
MmeI TCCRAC 1 cut(s) 391
MnlI CCTC 9 cut(s) 37, 151, 158, 310, 387, 456, 471, 506, 510
MroNI GCCGGC 1 cut(s) 341
MseI TTAA 3 cut(s) 129, 309, 562
MspI CCGG 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 338
NaeI GCCGGC 1 cut(s) 343
NdeI CATATG 1 cut(s) 13
NdeII GATC 3 cut(s) 186, 215, 256
NgoMIV GCCGGC 1 cut(s) 341
NlaIII CATG 3 cut(s) 254, 401, 452
NlaIV GGNNCC 1 cut(s) 375
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 1 cut(s) 46
NspI RCATGY 1 cut(s) 254
NspV TTCGAA 1 cut(s) 293
PaeI GCATGC 1 cut(s) 254
PdiI GCCGGC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 426
PflMI CCANNNNNTGG 1 cut(s) 203
PkrI GCNGC 2 cut(s) 117, 494
Psp124BI GAGCTC 1 cut(s) 394
PspN4I GGNNCC 1 cut(s) 375
PspPI GGNCC 1 cut(s) 458
PsrI GAACNNNNNNTAC 3 cut(s) 175, 207, 538
PsuI RGATCY 1 cut(s) 215
RsaI GTAC 1 cut(s) 9
RsaNI GTAC 1 cut(s) 8
SacI GAGCTC 1 cut(s) 394
SaqAI TTAA 3 cut(s) 129, 309, 562
SatI GCNGC 2 cut(s) 116, 493
Sau3AI GATC 3 cut(s) 186, 215, 256
Sau96I GGNCC 1 cut(s) 458
SduI GDGCHC 1 cut(s) 394
SfuI TTCGAA 1 cut(s) 293
SinI GGWCC 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 39
SmoI CTYRAG 1 cut(s) 39
SphI GCATGC 1 cut(s) 254
Sse9I AATT 3 cut(s) 52, 232, 453
SspMI CTAG 1 cut(s) 527
SstI GAGCTC 1 cut(s) 394
TaaI ACNGT 2 cut(s) 7, 134
TaiI ACGT 1 cut(s) 439
TaqI TCGA 1 cut(s) 293
TasI AATT 3 cut(s) 52, 232, 453
TfiI GAWTC 1 cut(s) 426
Tru1I TTAA 3 cut(s) 129, 309, 562
Tru9I TTAA 3 cut(s) 129, 309, 562
TscAI CASTG 2 cut(s) 139, 357
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 2 cut(s) 115, 492
Tsp45I GTSAC 1 cut(s) 46
TspRI CASTG 2 cut(s) 139, 357
Van91I CCANNNNNTGG 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 458
XapI RAATTY 2 cut(s) 52, 232
XceI RCATGY 1 cut(s) 254
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.