Rh7DG485800

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0013689
Physical Location & Seq
Forward (+)
69112574 .. 69113239
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG485800.1

Sequence Viewer

Length: 666 bp
ATGGCCAAGCCCAACGGAGAGGTCCTCCGTGCTCCGAATAGGGATCCGGACCGGGGCTTGATAATGGCCTTCAAAACCATCGAGACCATGTGTGATAGGTTAGGCCTTGTTTCCACCATCAAGAACCGAGCAAATGAGATGTACAAGACTCTTGAAGATCACAAGTGTAGCAAGGGAAGAAACCAAGATGCATTATTGGCTGCTTGCCTATACATTGCTTGTAAACAAGAAGACAAGCCCAGAACTCTCAAGGAAATCTTCTCCGTCGCGAATGGAGTCTCAAAGAAAGAGATTGGTAGAGCAAAGGAATACATAATCAAGCAACTGGGGTTAATGGAGAAGCCATTTCAAGTAGCAAATGCTGGGGACTATATGAGGCGATTCTGTTCCTCTCTTGGCTTGAACAATCAAGCAATCAAAGCAGCTCAACAAGCTGTGCACAAGTCGCAAGAAATTGATATAAGGAGGAGCCCTGTATCGATAGCCGCAGCAGTGATTTACATCATCACTCAGCTTTCAGATGATAGTAACAAGAGGCCTCTCAAAGATATCGCAGACGTTACAGGAGTAGCACCAGCAACTATCAAAAATTCATACAAAGATCTTAGTCCTCATGTATCTGAAATAATACCCAACTGGTATCATCTGAATTTGTCAAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

221

Amino Acids

24.65

Weight (kDa)

9.31

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIB PF00382 18 - 101 6.7e-22 Transcription factor TFIIB repeat
TFIIB PF00382 118 - 203 1.4e-12 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 269
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 1 cut(s) 486
AclWI GGATC 2 cut(s) 38, 51
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 589, 649
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 53
AgsI TTSAA 4 cut(s) 73, 155, 350, 403
AluBI AGCT 3 cut(s) 425, 434, 514
AluI AGCT 3 cut(s) 425, 434, 514
Alw21I GWGCWC 2 cut(s) 34, 441
Alw26I GTCTC 2 cut(s) 77, 283
Alw44I GTGCAC 1 cut(s) 437
AlwI GGATC 2 cut(s) 38, 51
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 4 cut(s) 3, 66, 103, 536
ApaLI GTGCAC 1 cut(s) 437
ApeKI GCWGC 3 cut(s) 200, 422, 488
ApoI RAATTY 2 cut(s) 589, 649
AspS9I GGNCC 2 cut(s) 22, 49
AsuC2I CCSGG 1 cut(s) 53
AvaII GGWCC 2 cut(s) 22, 49
BaeGI GKGCMC 1 cut(s) 441
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 43
BanII GRGCYC 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 2 cut(s) 34, 441
BbvI GCAGC 3 cut(s) 187, 434, 500
BccI CCATC 2 cut(s) 86, 125
BcnI CCSGG 1 cut(s) 53
BcoDI GTCTC 2 cut(s) 77, 283
BglII AGATCT 1 cut(s) 601
BisI GCNGC 4 cut(s) 201, 423, 486, 489
BlsI GCNGC 4 cut(s) 202, 424, 487, 490
Bme1390I CCNGG 1 cut(s) 53
Bme18I GGWCC 2 cut(s) 22, 49
BmgT120I GGNCC 2 cut(s) 22, 49
BmiI GGNNCC 2 cut(s) 45, 470
BmrFI CCNGG 1 cut(s) 53
BmrI ACTGGG 1 cut(s) 335
BmsI GCATC 1 cut(s) 178
BmuI ACTGGG 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 237
BplI GAGNNNNNCTC 2 cut(s) 9, 41
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 500
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 52
BsaWI WCCGGW 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 330, 641
Bse3DI GCAATG 1 cut(s) 213
Bse8I GATNNNNATC 1 cut(s) 500
BseAI TCCGGA 1 cut(s) 46
BseCI ATCGAT 1 cut(s) 479
BseDI CCNNGG 1 cut(s) 52
BseJI GATNNNNATC 1 cut(s) 500
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 524
BseNI ACTGG 2 cut(s) 330, 641
BseRI GAGGAG 1 cut(s) 481
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 3 cut(s) 187, 434, 500
BseYI CCCAGC 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 4 cut(s) 5, 68, 105, 538
BshVI ATCGAT 1 cut(s) 479
BsiHKAI GWGCWC 2 cut(s) 34, 441
BsiSI CCGG 2 cut(s) 47, 52
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 2 cut(s) 77, 283
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 4 cut(s) 5, 68, 105, 538
Bso31I GGTCTC 1 cut(s) 77
Bsp1286I GDGCHC 3 cut(s) 34, 441, 473
Bsp13I TCCGGA 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 3 cut(s) 43, 157, 601
Bsp68I TCGCGA 1 cut(s) 269
BspACI CCGC 1 cut(s) 486
BspANI GGCC 4 cut(s) 5, 68, 105, 538
BspCNI CTCAG 1 cut(s) 523
BspDI ATCGAT 1 cut(s) 479
BspEI TCCGGA 1 cut(s) 46
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 2 cut(s) 45, 470
BspPI GGATC 2 cut(s) 38, 51
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 213
BsrGI TGTACA 1 cut(s) 141
BsrI ACTGG 2 cut(s) 330, 641
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 3 cut(s) 43, 157, 601
BstAUI TGTACA 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 2 cut(s) 510, 605
BstFNI CGCG 1 cut(s) 269
BstKTI GATC 3 cut(s) 46, 160, 604
BstMAI GTCTC 2 cut(s) 77, 283
BstMBI GATC 3 cut(s) 43, 157, 601
BstMWI GCNNNNNNNGC 4 cut(s) 197, 419, 431, 445
BstSCI CCNGG 1 cut(s) 51
BstSLI GKGCMC 1 cut(s) 441
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 3 cut(s) 187, 434, 500
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 2 cut(s) 43, 601
BstYI RGATCY 2 cut(s) 43, 601
Bsu15I ATCGAT 1 cut(s) 479
BsuRI GGCC 4 cut(s) 5, 68, 105, 538
BsuTUI ATCGAT 1 cut(s) 479
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 498
BtuMI TCGCGA 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 205
Cfr13I GGNCC 2 cut(s) 22, 49
ClaI ATCGAT 1 cut(s) 479
CpoI CGGWCCG 1 cut(s) 49
Csp6I GTAC 1 cut(s) 142
CspI CGGWCCG 1 cut(s) 49
CviAII CATG 2 cut(s) 88, 614
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 2 cut(s) 510, 605
DpnI GATC 3 cut(s) 45, 159, 603
DpnII GATC 3 cut(s) 43, 157, 601
EaeI YGGCCR 1 cut(s) 3
Eco147I AGGCCT 2 cut(s) 105, 538
Eco24I GRGCYC 1 cut(s) 473
Eco31I GGTCTC 1 cut(s) 77
Eco32I GATATC 1 cut(s) 550
Eco47I GGWCC 2 cut(s) 22, 49
EcoO109I RGGNCCY 1 cut(s) 22
EcoRV GATATC 1 cut(s) 550
EcoT22I ATGCAT 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 473
FaeI CATG 2 cut(s) 91, 617
FaiI YATR 8 cut(s) 89, 211, 314, 372, 374, 461, 595, 615
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 1 cut(s) 380
FatI CATG 2 cut(s) 87, 613
Fnu4HI GCNGC 4 cut(s) 201, 423, 486, 489
FriOI GRGCYC 1 cut(s) 473
Fsp4HI GCNGC 4 cut(s) 201, 423, 486, 489
GluI GCNGC 4 cut(s) 201, 423, 486, 489
GsaI CCCAGC 1 cut(s) 366
HaeIII GGCC 4 cut(s) 5, 68, 105, 538
HapII CCGG 2 cut(s) 47, 52
Hin1II CATG 2 cut(s) 91, 617
HinfI GANTC 3 cut(s) 148, 276, 381
HpaII CCGG 2 cut(s) 47, 52
Hpy166II GTNNAC 2 cut(s) 224, 439
Hpy188I TCNGA 4 cut(s) 36, 520, 622, 648
Hpy188III TCNNGA 5 cut(s) 47, 82, 121, 152, 268
Hpy8I GTNNAC 2 cut(s) 224, 439
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 2 cut(s) 191, 439
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 419, 431, 445
HpyF3I CTNAG 2 cut(s) 510, 605
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 2 cut(s) 91, 617
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 3 cut(s) 43, 157, 601
LmnI GCTCC 2 cut(s) 37, 468
LpnPI CCDG 9 cut(s) 60, 65, 253, 311, 348, 486, 549, 588, 622
Lsp1109I GCAGC 3 cut(s) 187, 434, 500
LweI GCATC 1 cut(s) 178
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 2 cut(s) 527, 559
MalI GATC 3 cut(s) 45, 159, 603
MboI GATC 3 cut(s) 43, 157, 601
MboII GAAGA 4 cut(s) 167, 189, 242, 250
MflI RGATCY 2 cut(s) 43, 601
MhlI GDGCHC 3 cut(s) 34, 441, 473
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 453, 589, 649
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 142, 285
MnlI CCTC 8 cut(s) 13, 35, 369, 400, 459, 528, 549, 621
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 193
MroI TCCGGA 1 cut(s) 46
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 47, 52
MspR9I CCNGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 4 cut(s) 197, 419, 431, 445
NciI CCSGG 1 cut(s) 53
NdeII GATC 3 cut(s) 43, 157, 601
NlaIII CATG 2 cut(s) 91, 617
NlaIV GGNNCC 2 cut(s) 45, 470
NruI TCGCGA 1 cut(s) 269
NsiI ATGCAT 1 cut(s) 193
PceI AGGCCT 2 cut(s) 105, 538
PfeI GAWTC 1 cut(s) 381
PkrI GCNGC 4 cut(s) 202, 424, 487, 490
PleI GAGTC 2 cut(s) 142, 284
PpsI GAGTC 2 cut(s) 142, 284
PpuMI RGGWCCY 1 cut(s) 22
Psp5II RGGWCCY 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 362
PspN4I GGNNCC 2 cut(s) 45, 470
PspPI GGNCC 2 cut(s) 22, 49
PspPPI RGGWCCY 1 cut(s) 22
PsuI RGATCY 2 cut(s) 43, 601
RruI TCGCGA 1 cut(s) 269
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
Rsr2I CGGWCCG 1 cut(s) 49
RsrII CGGWCCG 1 cut(s) 49
SaqAI TTAA 1 cut(s) 332
SatI GCNGC 4 cut(s) 201, 423, 486, 489
Sau3AI GATC 3 cut(s) 43, 157, 601
Sau96I GGNCC 2 cut(s) 22, 49
SchI GAGTC 2 cut(s) 142, 285
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 3 cut(s) 34, 441, 473
SetI ASST 6 cut(s) 24, 101, 427, 436, 516, 561
SfaNI GCATC 1 cut(s) 178
SinI GGWCC 2 cut(s) 22, 49
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 3 cut(s) 453, 589, 649
SseBI AGGCCT 2 cut(s) 105, 538
SsiI CCGC 1 cut(s) 486
StuI AGGCCT 2 cut(s) 105, 538
StyD4I CCNGG 1 cut(s) 51
TaiI ACGT 1 cut(s) 561
TaqI TCGA 2 cut(s) 81, 479
TasI AATT 3 cut(s) 453, 589, 649
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 1 cut(s) 381
Tru1I TTAA 1 cut(s) 332
Tru9I TTAA 1 cut(s) 332
TscAI CASTG 1 cut(s) 498
TseI GCWGC 3 cut(s) 200, 422, 488
TspDTI ATGAA 1 cut(s) 582
TspGWI ACGGA 3 cut(s) 17, 30, 253
TspRI CASTG 1 cut(s) 498
VneI GTGCAC 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 22, 49
XapI RAATTY 2 cut(s) 589, 649
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.