RchiOBHm_Chr7g0241731

Pentatricopeptide repeat-containing protein At3g62470, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010920
Physical Location & Seq
Forward (+)
67758121 .. 67760427
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21663

Sequence Viewer

Length: 1968 bp
ATGTCAGTGTTTCTAAGAAGAAGCAAATTCAGCCAACAGTACCCGCTGTTTCTCCGCCGCCACTCTCAGTCAGATGTCTACCTACACCGCCGCTGCCGCTATCGCTCCGCCGCCGAGATGGGACGAAGCCCAGGAGGGCAGCAAAAACGAGGTCGCGGAGAACAAGGATTCCGTCCTACTGGTGGCTCTCTGCCCCTGTCTGGTTTGCTTAATTCTCCTCCTCATAGTAATCTCCATTGTCCTCATGGTCAAATTCCGTTTCTTTTGCCACACCCCACTTCGCTCTTTACTCTACAAGAAAAAATGCTAGCATCATCATCTTCAAACATCTCAAGTTTCTCAATTTCAATTAAACCCGTTGGTCATTTGCTACCAAATAGGGGCTTTTCTAGTGTCACCTCTGGTGGTGAGGAGGAGGAGGAGGAGGAGGAGGATGGAGGGGTTGAAAATGAGGATGTTAGTGTTAGTTCTGGTCCTGGTGCTGGTGCTGACCCGGAGGAGGTTGATAGGGTATGTAAGGTGATTGATGAATTGTTTGCATTGGATAGGAATATGGAGGCGGTTCTCGATCAATGTGGTGTTGAATTATCTCATGATTTAGTCGTTGCCGTCTTGAGACGCTTCCACCATGCTAGAAAACCTGCATTTCGGTTTTTCTGTTGGGCTGGGGACAAGCCTGGTTTTGAGCATGATTCCACGACTTATAACTCTATGATGAGTATTTTAGGGAAGACTAGACAGTTTGAGACTATGGTATCGCTGCTCGAAGAAATGGGAGTCAAGGGACTACTGACAATGGAAACTTTTGTGATTGCTTTCAAAGCTTTTGCTGCCGCCAAAGAGAGGAAGAAAGCTGTTGGAATCTTTGAGCTCATGAAGTATTACAAGTTCAAATATGGTGTCGATACCGTGAATTGCTTGCTTGATACTCTCGGAAGGGCAAAGCTAGGGAAAGAAATGCAAGTTCTTTTTGACAAGTTGAAAGGGAGGTTTACCCCAAATTTACAGACTTATGCGGTGCTGCTTGATGGGTGGTGCAGGGTGAAGAATTTAATGGAGGCGGGGAGGGTCTGGAACGAGATGATTGATAAGGGGTTTAAGCCTGATATTGTTACACATAATATCATGCTTGGAGGTTTGTTGAGGAGTCACAAGAGGTCTGATGCTATCAAATTGTTTGAGATCATGAAAGCCAAGGGTCCATCACCTAATGTTAGAACCTATAGTATTCTGATTCAGAACTTCTGCAAGCAAAAACAGATGAAAGAAGCAATAGACTTATTTGATGAAATGCGTGAATCTGGGTCCCAGCCTAGTGTTGTGGTTTACACAAGCTTAATCACAGGGTTTGGAAATCAGAACAAGATGGATATGGTTCATGGATTATTGAAAGCAATGAAGCAAGATGGTTGCACTCCTGATGGGGTGACCTACAATGTCTTGATCAAATTGATGACGAGGCAGAGAATGCCAGATGATGCGGTCAGGATATACAAGAAGATGATTCAAAATGGAGTTGGGCCATCAATACACACCTATGTCATGATAATGAAGTCTTATTATCAGACAAGAAATTATGACATGGGTCATGCAGTATGGGATGAGATGATTGAGAAGGGCTGCTGCCCAGATGATAACGCATATACTGTTCTCATTGGAGGTCTTATATGCCAGGGAAGAACGGGGGAGGCCTGCAAATATCTAGAGGAAATGATAGAGAAAGGAATGAAAATTCCTCAGCTTGACTTCAACAAGTTTGCCGCCGACTTCTCCAGAGCTGGGAAACCTGACATACTTGTGGAGATGGCTCAAAAGATGAAGGTCGCTGGTAAGTTTGAACTCTCCAATGTCTTTGCTAGGTGGGCTGAAATGACGAAGAAAAAGATGAAAGGAAGAGATCCTGACGAAACTAGTGATCAAGGAATCTATTTTACTTCCCATGGATCCATACATGATACTAGGTCTTAA

Protein Analysis

655

Amino Acids

73.78

Weight (kDa)

8.79

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 333 - 381 2.9e-12 PPR repeat family
PPR_3 PF13812 357 - 415 1.7e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 369 - 417 7.9e-14 PPR repeat family
TPR_24 PF23276 390 - 501 1.7e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 399 - 432 7.5e-11 PPR repeat
PPR PF01535 407 - 435 1.1e-07 PPR repeat
PPR_2 PF13041 410 - 451 1.1e-11 PPR repeat family
PPR_3 PF13812 427 - 485 1.1e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 438 - 483 5.4e-13 PPR repeat family
PPR_long PF17177 453 - 573 2.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 473 - 520 1.7e-10 PPR repeat family
PPR PF01535 476 - 506 8.3e-06 PPR repeat
PPR_2 PF13041 508 - 555 3.8e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010920)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62470 AT3G62540 AT5G14820
fragaria_vesca FvH4_5g39420
malus_domestica MD11G1315100.v1.1
prunus_persica Prupe.8G271400_v2.0.a1 Prupe.8G271400_v2.0.a1
pyrus_communis pycom03g23410 pycom11g27530
rosa_chinensis RchiOBHm_Chr7g0241731
rosa_laevigata RLG00000000549
rosa_multiflora Rmu_co8405791.1_g000001 Rmu_sc0002268.1_g000001
rosa_roxburghii Rroxscaffold_3G00218720
rosa_rugosa Rorug07G0346800
rosa_samantha Rh7AG501200 Rh7BG472500 Rh7CG517500 Rh7DG486200
rosa_wichuraiana Rw7G042360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
Acc36I ACCTGC 1 cut(s) 649
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 156
AclWI GGATC 3 cut(s) 1892, 1938, 1951
AcsI RAATTY 5 cut(s) 26, 252, 1000, 1048, 1731
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 7 cut(s) 182, 200, 380, 482, 499, 843, 1779
AhlI ACTAGT 1 cut(s) 1910
AjnI CCWGG 4 cut(s) 130, 475, 676, 1671
AloI GAACNNNNNNTCC 2 cut(s) 153, 185
AluBI AGCT 7 cut(s) 824, 854, 871, 946, 1335, 1741, 1778
AluI AGCT 7 cut(s) 824, 854, 871, 946, 1335, 1741, 1778
Alw21I GWGCWC 1 cut(s) 873
Alw26I GTCTC 2 cut(s) 610, 740
AlwI GGATC 3 cut(s) 1892, 1938, 1951
AoxI GGCC 2 cut(s) 1520, 1689
ApeKI GCWGC 7 cut(s) 93, 139, 760, 830, 1021, 1620, 1623
ApoI RAATTY 5 cut(s) 26, 252, 1000, 1048, 1731
ArsI GACNNNNNNTTYG 4 cut(s) 136, 168, 885, 917
AspS9I GGNCC 4 cut(s) 473, 1199, 1305, 1520
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 6 cut(s) 388, 419, 532, 1054, 1197, 1438
AsuNHI GCTAGC 1 cut(s) 307
AvaII GGWCC 3 cut(s) 473, 1199, 1305
BaeI ACNNNNGTAYC 2 cut(s) 738, 771
BamHI GGATCC 1 cut(s) 1943
BanII GRGCYC 1 cut(s) 873
BbsI GAAGAC 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 873
BbvCI CCTCAGC 1 cut(s) 1737
BbvI GCAGC 7 cut(s) 80, 151, 747, 817, 1008, 1607, 1610
BccI CCATC 9 cut(s) 112, 428, 1022, 1210, 1360, 1400, 1415, 1531, 1798
BceAI ACGGC 1 cut(s) 593
BciT130I CCWGG 4 cut(s) 132, 477, 678, 1673
BclI TGATCA 2 cut(s) 1443, 1915
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 2 cut(s) 610, 740
BcuI ACTAGT 1 cut(s) 1910
BfmI CTRYAG 1 cut(s) 1222
BfuAI ACCTGC 1 cut(s) 649
Bme1390I CCNGG 5 cut(s) 132, 477, 494, 678, 1673
Bme18I GGWCC 3 cut(s) 473, 1199, 1305
BmgT120I GGNCC 4 cut(s) 473, 1199, 1305, 1520
BmiI GGNNCC 4 cut(s) 1200, 1306, 1307, 1945
BmrFI CCNGG 5 cut(s) 132, 477, 494, 678, 1673
BmsI GCATC 3 cut(s) 320, 1153, 1468
BmtI GCTAGC 1 cut(s) 311
BoxI GACNNNNGTC 1 cut(s) 1584
BpiI GAAGAC 1 cut(s) 737
BpmI CTGGAG 1 cut(s) 1756
Bpu10I CCTNAGC 1 cut(s) 1737
BpuEI CTTGAG 2 cut(s) 316, 634
BpuMI CCSGG 1 cut(s) 494
BsaJI CCNNGG 4 cut(s) 130, 1194, 1672, 1939
Bsc4I CCNNNNNNNGG 7 cut(s) 182, 200, 380, 482, 499, 843, 1779
Bse1I ACTGG 1 cut(s) 184
Bse3DI GCAATG 1 cut(s) 1401
BseBI CCWGG 4 cut(s) 132, 477, 678, 1673
BseDI CCNNGG 4 cut(s) 130, 1194, 1672, 1939
BseGI GGATG 3 cut(s) 439, 460, 1606
BseLI CCNNNNNNNGG 7 cut(s) 182, 200, 380, 482, 499, 843, 1779
BseMI GCAATG 1 cut(s) 1401
BseMII CTCAG 2 cut(s) 80, 1751
BseNI ACTGG 1 cut(s) 184
BseXI GCAGC 7 cut(s) 80, 151, 747, 817, 1008, 1607, 1610
BseYI CCCAGC 3 cut(s) 665, 1308, 1778
BsgI GTGCAG 1 cut(s) 1057
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 2 cut(s) 1522, 1691
BsiHKAI GWGCWC 1 cut(s) 873
BsiSI CCGG 1 cut(s) 494
BslFI GGGAC 4 cut(s) 135, 683, 798, 1291
BslI CCNNNNNNNGG 7 cut(s) 182, 200, 380, 482, 499, 843, 1779
BsmAI GTCTC 2 cut(s) 610, 740
BsmBI CGTCTC 1 cut(s) 610
BsmFI GGGAC 4 cut(s) 135, 683, 798, 1291
BsmI GAATGC 1 cut(s) 1473
BsnI GGCC 2 cut(s) 1522, 1691
Bsp1286I GDGCHC 1 cut(s) 873
Bsp143I GATC 6 cut(s) 568, 1182, 1443, 1897, 1915, 1943
Bsp19I CCATGG 1 cut(s) 1939
BspANI GGCC 2 cut(s) 1522, 1691
BspCNI CTCAG 2 cut(s) 79, 1750
BspFNI CGCG 1 cut(s) 156
BspHI TCATGA 4 cut(s) 592, 873, 1185, 1542
BspLI GGNNCC 4 cut(s) 1200, 1306, 1307, 1945
BspMI ACCTGC 1 cut(s) 649
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 3 cut(s) 1892, 1938, 1951
BsrDI GCAATG 1 cut(s) 1401
BsrI ACTGG 1 cut(s) 184
BssECI CCNNGG 4 cut(s) 130, 1194, 1672, 1939
BssMI GATC 6 cut(s) 568, 1182, 1443, 1897, 1915, 1943
BssT1I CCWWGG 2 cut(s) 1194, 1939
Bst2UI CCWGG 4 cut(s) 132, 477, 678, 1673
Bst4CI ACNGT 4 cut(s) 39, 741, 910, 1648
Bst6I CTCTTC 1 cut(s) 1888
BstAPI GCANNNNNTGC 1 cut(s) 1468
BstC8I GCNNGC 4 cut(s) 309, 920, 1250, 1693
BstDEI CTNAG 3 cut(s) 14, 66, 1737
BstDSI CCRYGG 1 cut(s) 1939
BstEII GGTNACC 1 cut(s) 1426
BstF5I GGATG 3 cut(s) 439, 460, 1606
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 6 cut(s) 571, 1185, 1446, 1900, 1918, 1946
BstMAI GTCTC 2 cut(s) 610, 740
BstMBI GATC 6 cut(s) 568, 1182, 1443, 1897, 1915, 1943
BstMWI GCNNNNNNNGC 7 cut(s) 30, 96, 102, 821, 830, 1468, 1862
BstNI CCWGG 4 cut(s) 132, 477, 678, 1673
BstPAI GACNNNNGTC 1 cut(s) 1584
BstPI GGTNACC 1 cut(s) 1426
BstSCI CCNGG 5 cut(s) 130, 475, 492, 676, 1671
BstSFI CTRYAG 1 cut(s) 1222
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 7 cut(s) 80, 151, 747, 817, 1008, 1607, 1610
BstV2I GAAGAC 1 cut(s) 737
BstX2I RGATCY 2 cut(s) 1897, 1943
BstYI RGATCY 2 cut(s) 1897, 1943
BsuRI GGCC 2 cut(s) 1522, 1691
BtgI CCRYGG 1 cut(s) 1939
BtsCI GGATG 3 cut(s) 439, 460, 1606
BtsIMutI CAGTG 1 cut(s) 12
BveI ACCTGC 1 cut(s) 649
Cac8I GCNNGC 4 cut(s) 309, 920, 1250, 1693
CciI TCATGA 4 cut(s) 592, 873, 1185, 1542
Cfr13I GGNCC 4 cut(s) 473, 1199, 1305, 1520
CseI GACGC 1 cut(s) 627
Csp6I GTAC 1 cut(s) 40
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 3 cut(s) 14, 66, 1737
DpnI GATC 6 cut(s) 570, 1184, 1445, 1899, 1917, 1945
DpnII GATC 6 cut(s) 568, 1182, 1443, 1897, 1915, 1943
Eam1104I CTCTTC 1 cut(s) 1888
EarI CTCTTC 1 cut(s) 1888
EciI GGCGGA 2 cut(s) 44, 97
Ecl136II GAGCTC 1 cut(s) 871
Eco130I CCWWGG 2 cut(s) 1194, 1939
Eco147I AGGCCT 1 cut(s) 1691
Eco24I GRGCYC 1 cut(s) 873
Eco47I GGWCC 3 cut(s) 473, 1199, 1305
Eco53kI GAGCTC 1 cut(s) 871
Eco91I GGTNACC 1 cut(s) 1426
EcoICRI GAGCTC 1 cut(s) 871
EcoO109I RGGNCCY 1 cut(s) 1305
EcoO65I GGTNACC 1 cut(s) 1426
EcoRII CCWGG 4 cut(s) 130, 475, 676, 1671
EcoT14I CCWWGG 2 cut(s) 1194, 1939
EcoT38I GRGCYC 1 cut(s) 873
ErhI CCWWGG 2 cut(s) 1194, 1939
Esp3I CGTCTC 1 cut(s) 610
FaqI GGGAC 4 cut(s) 135, 683, 798, 1291
FauI CCCGC 2 cut(s) 51, 1054
FbaI TGATCA 2 cut(s) 1443, 1915
FblI GTMKAC 1 cut(s) 78
FokI GGATG 3 cut(s) 446, 467, 1613
FriOI GRGCYC 1 cut(s) 873
GsaI CCCAGC 3 cut(s) 669, 1312, 1782
GsuI CTGGAG 1 cut(s) 1756
HaeIII GGCC 2 cut(s) 1522, 1691
HapII CCGG 1 cut(s) 494
HgaI GACGC 1 cut(s) 627
HindIII AAGCTT 2 cut(s) 822, 1333
HinfI GANTC 9 cut(s) 168, 692, 777, 861, 1147, 1234, 1298, 1504, 1923
HpaII CCGG 1 cut(s) 494
HphI GGTGA 6 cut(s) 388, 419, 532, 1054, 1197, 1438
Hpy166II GTNNAC 3 cut(s) 79, 993, 1327
Hpy188I TCNGA 7 cut(s) 73, 935, 1162, 1233, 1239, 1359, 1566
Hpy8I GTNNAC 3 cut(s) 79, 993, 1327
HpyAV CCTTC 3 cut(s) 930, 1609, 1813
HpyCH4III ACNGT 4 cut(s) 39, 741, 910, 1648
HpyCH4V TGCA 8 cut(s) 539, 644, 961, 1038, 1248, 1413, 1592, 1695
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 96, 102, 821, 830, 1468, 1862
HpyF3I CTNAG 3 cut(s) 14, 66, 1737
KflI GGGWCCC 1 cut(s) 1305
Ksp22I TGATCA 2 cut(s) 1443, 1915
Kzo9I GATC 6 cut(s) 568, 1182, 1443, 1897, 1915, 1943
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 7 cut(s) 80, 151, 747, 817, 1008, 1607, 1610
LweI GCATC 3 cut(s) 320, 1153, 1468
MaeIII GTNAC 4 cut(s) 394, 1111, 1148, 1426
MalI GATC 6 cut(s) 570, 1184, 1445, 1899, 1917, 1945
MboI GATC 6 cut(s) 568, 1182, 1443, 1897, 1915, 1943
MflI RGATCY 2 cut(s) 1897, 1943
MhlI GDGCHC 1 cut(s) 873
MlyI GAGTC 2 cut(s) 786, 1156
MmeI TCCRAC 1 cut(s) 838
MseI TTAA 6 cut(s) 210, 351, 1052, 1098, 1337, 1966
MslI CAYNNNNRTG 3 cut(s) 1536, 1547, 1796
MspA1I CMGCKG 2 cut(s) 46, 93
MspI CCGG 1 cut(s) 494
MspR9I CCNGG 5 cut(s) 132, 477, 494, 678, 1673
Mva1269I GAATGC 1 cut(s) 1473
MvaI CCWGG 4 cut(s) 132, 477, 678, 1673
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 7 cut(s) 30, 96, 102, 821, 830, 1468, 1862
NciI CCSGG 1 cut(s) 494
NcoI CCATGG 1 cut(s) 1939
NdeII GATC 6 cut(s) 568, 1182, 1443, 1897, 1915, 1943
NheI GCTAGC 1 cut(s) 307
NlaIV GGNNCC 4 cut(s) 1200, 1306, 1307, 1945
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 3 cut(s) 394, 1148, 1426
PagI TCATGA 4 cut(s) 592, 873, 1185, 1542
PceI AGGCCT 1 cut(s) 1691
PctI GAATGC 1 cut(s) 1473
PfeI GAWTC 7 cut(s) 168, 692, 861, 1234, 1298, 1504, 1923
PleI GAGTC 2 cut(s) 785, 1155
PpsI GAGTC 2 cut(s) 785, 1155
PpuMI RGGWCCY 1 cut(s) 1305
PshAI GACNNNNGTC 1 cut(s) 1584
PsiI TTATAA 1 cut(s) 705
Psp124BI GAGCTC 1 cut(s) 873
Psp5II RGGWCCY 1 cut(s) 1305
Psp6I CCWGG 4 cut(s) 130, 475, 676, 1671
PspEI GGTNACC 1 cut(s) 1426
PspFI CCCAGC 3 cut(s) 665, 1308, 1778
PspGI CCWGG 4 cut(s) 130, 475, 676, 1671
PspN4I GGNNCC 4 cut(s) 1200, 1306, 1307, 1945
PspPI GGNCC 4 cut(s) 473, 1199, 1305, 1520
PspPPI RGGWCCY 1 cut(s) 1305
PsrI GAACNNNNNNTAC 2 cut(s) 872, 904
PsuI RGATCY 2 cut(s) 1897, 1943
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 3 cut(s) 1536, 1547, 1796
SacI GAGCTC 1 cut(s) 873
SaqAI TTAA 6 cut(s) 210, 351, 1052, 1098, 1337, 1966
Sau3AI GATC 6 cut(s) 568, 1182, 1443, 1897, 1915, 1943
Sau96I GGNCC 4 cut(s) 473, 1199, 1305, 1520
SchI GAGTC 2 cut(s) 786, 1156
ScrFI CCNGG 5 cut(s) 132, 477, 494, 678, 1673
SduI GDGCHC 1 cut(s) 873
SfaNI GCATC 3 cut(s) 320, 1153, 1468
SfcI CTRYAG 1 cut(s) 1222
SinI GGWCC 3 cut(s) 473, 1199, 1305
SmiMI CAYNNNNRTG 3 cut(s) 1536, 1547, 1796
SmlI CTYRAG 2 cut(s) 331, 613
SmoI CTYRAG 2 cut(s) 331, 613
SpeI ACTAGT 1 cut(s) 1910
SseBI AGGCCT 1 cut(s) 1691
SstI GAGCTC 1 cut(s) 873
StuI AGGCCT 1 cut(s) 1691
StyD4I CCNGG 5 cut(s) 130, 475, 492, 676, 1671
StyI CCWWGG 2 cut(s) 1194, 1939
TaaI ACNGT 4 cut(s) 39, 741, 910, 1648
TaqI TCGA 3 cut(s) 567, 765, 903
TauI GCSGC 6 cut(s) 60, 93, 99, 113, 836, 1763
TfiI GAWTC 7 cut(s) 168, 692, 861, 1234, 1298, 1504, 1923
Tru1I TTAA 6 cut(s) 210, 351, 1052, 1098, 1337, 1966
Tru9I TTAA 6 cut(s) 210, 351, 1052, 1098, 1337, 1966
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 3 cut(s) 394, 1148, 1426
TseI GCWGC 7 cut(s) 93, 139, 760, 830, 1021, 1620, 1623
Tsp45I GTSAC 3 cut(s) 394, 1148, 1426
TspGWI ACGGA 2 cut(s) 161, 246
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 3 cut(s) 473, 1199, 1305
XapI RAATTY 5 cut(s) 26, 252, 1000, 1048, 1731
XbaI TCTAGA 1 cut(s) 1702
XcmI CCANNNNNNNNNTGG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.