RLG00000000643

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016966
Physical Location & Seq
Forward (+)
3025299 .. 3026708
1410 bp
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UTR
Exon/CDS
Intron
RLM00000000643

Sequence Viewer

Length: 396 bp
ATGAATGTACAAGTTGCTCTTGGTTTGATTTCAGATCCAAAGGAGGAGGCTAAATCAGAGACAAGGGATTGGATAAACACTGTGGTTCTCACCATTGGATCTCTTTATCAGGATGATTCTGCTGCACTTAGGAGTGCTGCTTCTGCTTGGCACGCTGAACTGGCACCGCTCCCAAAGCCCTTGCTTGTAGTCAATATCGGAGGCCCTTCTGACTGCAGTAGTAGTAATGGCAGATTGGGGACCGGTGGTGATAGCGGTGGTGTTGTTTGTGCTGTTAAGTCCAAGGCTACTGTTTCAGCTGCTGGGGAAGAGCAGAGTGGTGGAGTTTGGCAACATGCAAACCAGCGGACTATAGTTGTATTATATGCTCTTAAGAACCTGTGCCTCTGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mito_fiss_Elm1

Protein Analysis

132

Amino Acids

13.61

Weight (kDa)

5.57

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 23 - 72 2.2e-09 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 163
AccBSI CCGCTC 1 cut(s) 169
AciI CCGC 3 cut(s) 167, 255, 346
AclWI GGATC 2 cut(s) 29, 106
AfaI GTAC 1 cut(s) 9
AflII CTTAAG 1 cut(s) 371
AgeI ACCGGT 1 cut(s) 242
AluBI AGCT 1 cut(s) 299
AluI AGCT 1 cut(s) 299
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 2 cut(s) 29, 106
AlwNI CAGNNNCTG 1 cut(s) 302
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 3 cut(s) 122, 137, 299
AsiGI ACCGGT 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 203, 240
AsuHPI GGTGA 2 cut(s) 82, 260
AvaII GGWCC 1 cut(s) 240
BanI GGYRCC 1 cut(s) 163
BbvI GCAGC 3 cut(s) 109, 124, 286
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 1 cut(s) 394
BfmI CTRYAG 2 cut(s) 214, 351
BfrI CTTAAG 1 cut(s) 371
BisI GCNGC 3 cut(s) 123, 138, 300
BlsI GCNGC 3 cut(s) 124, 139, 301
Bme18I GGWCC 1 cut(s) 240
BmgT120I GGNCC 2 cut(s) 203, 240
BmiI GGNNCC 2 cut(s) 165, 241
BsaJI CCNNGG 1 cut(s) 282
BsaWI WCCGGW 1 cut(s) 242
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 282
BseGI GGATG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 165
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 3 cut(s) 109, 124, 286
BseYI CCCAGC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 108
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 163
BshTI ACCGGT 1 cut(s) 242
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 253
BsmAI GTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 253
BsnI GGCC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 7
Bsp143I GATC 2 cut(s) 34, 98
BspACI CCGC 3 cut(s) 167, 255, 346
BspANI GGCC 1 cut(s) 204
BspLI GGNNCC 2 cut(s) 165, 241
BspMAI CTGCAG 1 cut(s) 218
BspPI GGATC 2 cut(s) 29, 106
BspQI GCTCTTC 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 163
BspTI CTTAAG 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 7
BsrI ACTGG 1 cut(s) 165
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 2 cut(s) 34, 98
BssT1I CCWWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 82, 292
Bst6I CTCTTC 1 cut(s) 303
BstAFI CTTAAG 1 cut(s) 371
BstAUI TGTACA 1 cut(s) 7
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 128
BstF5I GGATG 1 cut(s) 118
BstKTI GATC 2 cut(s) 37, 101
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 2 cut(s) 34, 98
BstMWI GCNNNNNNNGC 4 cut(s) 143, 152, 161, 175
BstNSI RCATGY 1 cut(s) 338
BstSFI CTRYAG 2 cut(s) 214, 351
BstV1I GCAGC 3 cut(s) 109, 124, 286
BstX2I RGATCY 2 cut(s) 34, 98
BstYI RGATCY 2 cut(s) 34, 98
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 1 cut(s) 153
CaiI CAGNNNCTG 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 203, 240
Csp6I GTAC 1 cut(s) 8
CspAI ACCGGT 1 cut(s) 242
CviAII CATG 1 cut(s) 335
CviJI RGCY 5 cut(s) 50, 178, 204, 287, 299
CviKI_1 RGCY 5 cut(s) 50, 178, 204, 287, 299
CviQI GTAC 1 cut(s) 8
DdeI CTNAG 1 cut(s) 128
DpnI GATC 2 cut(s) 36, 100
DpnII GATC 2 cut(s) 34, 98
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 240
EcoO109I RGGNCCY 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 1 cut(s) 338
FaiI YATR 4 cut(s) 336, 353, 364, 366
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 1 cut(s) 253
FatI CATG 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 123, 138, 300
FokI GGATG 1 cut(s) 125
Fsp4HI GCNGC 3 cut(s) 123, 138, 300
FspBI CTAG 1 cut(s) 394
GluI GCNGC 3 cut(s) 123, 138, 300
GsaI CCCAGC 1 cut(s) 306
HaeIII GGCC 1 cut(s) 204
HapII CCGG 1 cut(s) 243
Hin1II CATG 1 cut(s) 338
HinfI GANTC 1 cut(s) 116
HpaII CCGG 1 cut(s) 243
HphI GGTGA 2 cut(s) 82, 260
Hpy188I TCNGA 4 cut(s) 34, 58, 200, 211
Hpy188III TCNNGA 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 2 cut(s) 82, 292
HpyCH4V TGCA 3 cut(s) 125, 216, 338
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 152, 161, 175
HpyF3I CTNAG 1 cut(s) 128
Hsp92II CATG 1 cut(s) 338
Kzo9I GATC 2 cut(s) 34, 98
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 1 cut(s) 174
LpnPI CCDG 6 cut(s) 95, 146, 256, 288, 356, 392
Lsp1109I GCAGC 3 cut(s) 109, 124, 286
MaeI CTAG 1 cut(s) 394
MalI GATC 2 cut(s) 36, 100
MbiI CCGCTC 1 cut(s) 169
MboI GATC 2 cut(s) 34, 98
MboII GAAGA 1 cut(s) 320
MflI RGATCY 2 cut(s) 34, 98
MnlI CCTC 4 cut(s) 37, 40, 194, 395
MseI TTAA 2 cut(s) 276, 372
MspA1I CMGCKG 2 cut(s) 299, 346
MspCI CTTAAG 1 cut(s) 371
MspI CCGG 1 cut(s) 243
MwoI GCNNNNNNNGC 4 cut(s) 143, 152, 161, 175
NdeII GATC 2 cut(s) 34, 98
NlaIII CATG 1 cut(s) 338
NlaIV GGNNCC 2 cut(s) 165, 241
NspI RCATGY 1 cut(s) 338
PciSI GCTCTTC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 116
PinAI ACCGGT 1 cut(s) 242
PkrI GCNGC 3 cut(s) 124, 139, 301
PspFI CCCAGC 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 165, 241
PspPI GGNCC 2 cut(s) 203, 240
PstI CTGCAG 1 cut(s) 218
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 2 cut(s) 34, 98
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 1 cut(s) 9
RsaNI GTAC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 276, 372
SatI GCNGC 3 cut(s) 123, 138, 300
Sau3AI GATC 2 cut(s) 34, 98
Sau96I GGNCC 2 cut(s) 203, 240
SetI ASST 2 cut(s) 301, 381
SfcI CTRYAG 2 cut(s) 214, 351
SinI GGWCC 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SsiI CCGC 3 cut(s) 167, 255, 346
SspMI CTAG 1 cut(s) 394
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 82, 292
TatI WGTACW 1 cut(s) 7
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 2 cut(s) 276, 372
Tru9I TTAA 2 cut(s) 276, 372
TscAI CASTG 1 cut(s) 85
TseI GCWGC 3 cut(s) 122, 137, 299
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 85
Vha464I CTTAAG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 240
XceI RCATGY 1 cut(s) 338
XspI CTAG 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.