RLG00000002268
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017470
Physical Location & Seq
Forward (+)
31005160 .. 31005657
498 bp
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UTR
Exon/CDS
Intron
RLM00000002268

Sequence Viewer

Length: 498 bp
ATGGATCTTTGTCGTAAGCGTGCTAGAAAAGAAGAGGAGGAGGACGTGGTGGTGGAGGATGAAGAAAAGCTAGGGAAAATGAAAGCCTTTTTGGCTGCTGTAACTGGATTTATAGCAGCAATAGTAGCTTGGTACCATTGTAAGTTACTCACAAGAAGAGAGTCACTGCCTGATTGGGATGAACACAGACGATTCTATTTGAATCGATTAAGTGGATCTGAAGTTGAGTGTATGAAAAACTTAAGAGTTAGCATGTATGGATTTTCAAAACTTTGCCAAGTCGTATATGACAAAGGTGGATTACGAAGAACAAGACATGTATCTATTGAAGAATCTGTATTTCGCGAAATCAAAGAAGAGGAGATTAGAACTGTTGAAGCCACTAATGAGTGGACTGTATTTCATGATCAATTGGTGATGGATATGTTTCTTAACTATCAAAGGCATCGAGCTCAACAAGCGCAACAAGAACAAGGAGACCTTGCAAATGTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

166

Amino Acids

19.53

Weight (kDa)

6.0

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 68 - 113 2.9e-07 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35540
pyrus_communis pycom111g03670
rosa_laevigata RLG00000002268 RLG00000020968
rosa_multiflora Rmu_sc0006282.1_g000017 Rmu_sc0008094.1_g000004
rosa_roxburghii Rroxscaffold_6G00393390
rosa_rugosa Rorug05G0325700 Rorug05G0325800 Rorug05G0325800
rosa_samantha Rh1AG000700 Rh1DG000500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 132
AccB1I GGYRCC 1 cut(s) 132
AccII CGCG 1 cut(s) 345
AclWI GGATC 2 cut(s) 12, 223
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 1 cut(s) 134
AflII CTTAAG 1 cut(s) 241
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 4 cut(s) 202, 267, 329, 377
AjiI CACGTC 1 cut(s) 46
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 3 cut(s) 70, 128, 452
AluI AGCT 3 cut(s) 70, 128, 452
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 1 cut(s) 471
AlwI GGATC 2 cut(s) 12, 223
ApeKI GCWGC 2 cut(s) 95, 116
Asp718I GGTACC 1 cut(s) 132
AspLEI GCGC 1 cut(s) 463
AsuHPI GGTGA 1 cut(s) 427
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BanI GGYRCC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 454
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 2 cut(s) 82, 128
BccI CCATC 1 cut(s) 412
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 471
BfaI CTAG 2 cut(s) 24, 71
BfrI CTTAAG 1 cut(s) 241
BglI GCCNNNNNGGC 1 cut(s) 92
BisI GCNGC 2 cut(s) 96, 117
BlsI GCNGC 2 cut(s) 97, 118
BmgBI CACGTC 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 134
BmsI GCATC 1 cut(s) 454
Bsa29I ATCGAT 1 cut(s) 205
BsaI GGTCTC 1 cut(s) 471
Bse1I ACTGG 1 cut(s) 109
BseCI ATCGAT 1 cut(s) 205
BseGI GGATG 2 cut(s) 64, 184
BseNI ACTGG 1 cut(s) 109
BseRI GAGGAG 3 cut(s) 50, 53, 374
BseXI GCAGC 2 cut(s) 82, 128
Bsh1236I CGCG 1 cut(s) 345
BshNI GGYRCC 1 cut(s) 132
BshVI ATCGAT 1 cut(s) 205
BsiHKAI GWGCWC 1 cut(s) 454
BsmAI GTCTC 1 cut(s) 471
Bso31I GGTCTC 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 454
Bsp143I GATC 3 cut(s) 4, 215, 406
Bsp68I TCGCGA 1 cut(s) 345
BspDI ATCGAT 1 cut(s) 205
BspFNI CGCG 1 cut(s) 345
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 134
BspPI GGATC 2 cut(s) 12, 223
BspT107I GGYRCC 1 cut(s) 132
BspTI CTTAAG 1 cut(s) 241
BspTNI GGTCTC 1 cut(s) 471
BsrI ACTGG 1 cut(s) 109
BssMI GATC 3 cut(s) 4, 215, 406
Bst4CI ACNGT 2 cut(s) 373, 397
Bst6I CTCTTC 3 cut(s) 27, 151, 351
BstAFI CTTAAG 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 21
BstF5I GGATG 2 cut(s) 64, 184
BstFNI CGCG 1 cut(s) 345
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 3 cut(s) 7, 218, 409
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 3 cut(s) 4, 215, 406
BstMWI GCNNNNNNNGC 3 cut(s) 92, 125, 458
BstNSI RCATGY 2 cut(s) 256, 320
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 82, 128
BstX2I RGATCY 2 cut(s) 4, 215
BstYI RGATCY 2 cut(s) 4, 215
Bsu15I ATCGAT 1 cut(s) 205
BsuTUI ATCGAT 1 cut(s) 205
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 2 cut(s) 64, 184
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 1 cut(s) 164
BtuMI TCGCGA 1 cut(s) 345
Cac8I GCNNGC 1 cut(s) 21
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 463
ClaI ATCGAT 1 cut(s) 205
Csp6I GTAC 1 cut(s) 133
CviAII CATG 3 cut(s) 253, 317, 404
CviJI RGCY 6 cut(s) 70, 86, 95, 128, 380, 452
CviKI_1 RGCY 6 cut(s) 70, 86, 95, 128, 380, 452
CviQI GTAC 1 cut(s) 133
DpnI GATC 3 cut(s) 6, 217, 408
DpnII GATC 3 cut(s) 4, 215, 406
Eam1104I CTCTTC 3 cut(s) 27, 151, 351
EarI CTCTTC 3 cut(s) 27, 151, 351
Ecl136II GAGCTC 1 cut(s) 452
Eco24I GRGCYC 1 cut(s) 454
Eco31I GGTCTC 1 cut(s) 471
Eco53kI GAGCTC 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 454
FaeI CATG 3 cut(s) 256, 320, 407
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FatI CATG 3 cut(s) 252, 316, 403
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 2 cut(s) 96, 117
FokI GGATG 2 cut(s) 71, 191
FriOI GRGCYC 1 cut(s) 454
Fsp4HI GCNGC 2 cut(s) 96, 117
FspBI CTAG 2 cut(s) 24, 71
GlaI GCGC 1 cut(s) 462
GluI GCNGC 2 cut(s) 96, 117
HhaI GCGC 1 cut(s) 463
Hin1II CATG 3 cut(s) 256, 320, 407
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HinfI GANTC 4 cut(s) 161, 192, 202, 332
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 2 cut(s) 344, 404
Hpy8I GTNNAC 1 cut(s) 393
HpyCH4III ACNGT 2 cut(s) 373, 397
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 1 cut(s) 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 125, 458
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 3 cut(s) 256, 320, 407
HspAI GCGC 1 cut(s) 461
KpnI GGTACC 1 cut(s) 136
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 3 cut(s) 4, 215, 406
LpnPI CCDG 2 cut(s) 90, 183
Lsp1109I GCAGC 2 cut(s) 82, 128
LweI GCATC 1 cut(s) 454
MaeI CTAG 2 cut(s) 24, 71
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 3 cut(s) 100, 144, 162
MalI GATC 3 cut(s) 6, 217, 408
MboI GATC 3 cut(s) 4, 215, 406
MboII GAAGA 6 cut(s) 44, 74, 168, 318, 341, 368
MfeI CAATTG 1 cut(s) 410
MflI RGATCY 2 cut(s) 4, 215
MhlI GDGCHC 1 cut(s) 454
MluCI AATT 1 cut(s) 410
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 5 cut(s) 28, 31, 34, 49, 352
MseI TTAA 3 cut(s) 209, 242, 432
MspCI CTTAAG 1 cut(s) 241
MunI CAATTG 1 cut(s) 410
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 92, 125, 458
NdeII GATC 3 cut(s) 4, 215, 406
NlaIII CATG 3 cut(s) 256, 320, 407
NlaIV GGNNCC 1 cut(s) 134
NmuCI GTSAC 1 cut(s) 162
NruI TCGCGA 1 cut(s) 345
NspI RCATGY 2 cut(s) 256, 320
PagI TCATGA 1 cut(s) 403
PciI ACATGT 1 cut(s) 316
PfeI GAWTC 3 cut(s) 192, 202, 332
PkrI GCNGC 2 cut(s) 97, 118
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PscI ACATGT 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 454
PspN4I GGNNCC 1 cut(s) 134
PsuI RGATCY 2 cut(s) 4, 215
RruI TCGCGA 1 cut(s) 345
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 3 cut(s) 209, 242, 432
SatI GCNGC 2 cut(s) 96, 117
Sau3AI GATC 3 cut(s) 4, 215, 406
SchI GAGTC 1 cut(s) 170
SduI GDGCHC 1 cut(s) 454
SetI ASST 6 cut(s) 48, 72, 130, 298, 454, 483
SfaNI GCATC 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 1 cut(s) 410
SspMI CTAG 2 cut(s) 24, 71
SstI GAGCTC 1 cut(s) 454
TaaI ACNGT 2 cut(s) 373, 397
TaiI ACGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 205, 448
TasI AATT 1 cut(s) 410
TfiI GAWTC 3 cut(s) 192, 202, 332
Tru1I TTAA 3 cut(s) 209, 242, 432
Tru9I TTAA 3 cut(s) 209, 242, 432
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 1 cut(s) 162
TseI GCWGC 2 cut(s) 95, 116
Tsp45I GTSAC 1 cut(s) 162
TspDTI ATGAA 5 cut(s) 75, 95, 195, 248, 392
TspRI CASTG 1 cut(s) 171
Vha464I CTTAAG 1 cut(s) 241
XceI RCATGY 2 cut(s) 256, 320
XspI CTAG 2 cut(s) 24, 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.