Rroxscaffold_6G00393390

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017470
Physical Location & Seq
Forward (+)
13425171 .. 13439979
14809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393390.1

Sequence Viewer

Length: 573 bp
ATGTCGTTCGATGAATTTCCTCCTGAACGAAAAGAAGATCTGATAGACAATCACGAAGATAATTTTGAATGGGATGATTTTCAAACCCAAGATCAGCAAAGAGAGAATGCTAATGAATGCATCAACCTAGAGGTTGATTTGGTGGATCAAGGTTCAAATCTTCCGCTGATAGAGGGAAAACGTGTGTTTGAGGAGGCGTTGACTTCGGGGATGGATGTCACTAATGGGGTTTTCCGGCAATCTCCGGTGGTGAAACTTTCCGGATCGACTCGTCTCCATCGTGCCCTCCTTTCGTGGCATCCTCTAGCGGAGATATTCACCGTGTGTAGAGCGAGCAAGGCGGCGAAGTTTGGTGTATTTGGACCGGGTCGGAAAACGCGGCTCTGCGACGGCACGGCTTCAAAATTTCTTCGAGGTGGGCCCCGCAAGGCCACTTTGGATTTCAATGTGAAATCCTGGACTGGTCTGTCAACATATCCACCAAGCCAAACCATTCCAAAGCTTCAAAGAATACCCAAAAGTTCCAAAACCAAGCTCTACATAAACCCTAGTTTGTTTAATGAAAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

190

Amino Acids

21.54

Weight (kDa)

7.7

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35540
pyrus_communis pycom111g03670
rosa_laevigata RLG00000002268 RLG00000020968
rosa_multiflora Rmu_sc0006282.1_g000017 Rmu_sc0008094.1_g000004
rosa_roxburghii Rroxscaffold_6G00393390
rosa_rugosa Rorug05G0325700 Rorug05G0325800 Rorug05G0325800
rosa_samantha Rh1AG000700 Rh1DG000500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 466
AccII CGCG 1 cut(s) 379
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 5 cut(s) 164, 308, 341, 379, 424
AclWI GGATC 2 cut(s) 153, 271
AcsI RAATTY 2 cut(s) 14, 404
AdeI CACNNNGTG 1 cut(s) 324
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 6 cut(s) 68, 83, 156, 402, 445, 506
AjnI CCWGG 1 cut(s) 455
AluBI AGCT 2 cut(s) 502, 535
AluI AGCT 2 cut(s) 502, 535
Alw26I GTCTC 1 cut(s) 278
AlwI GGATC 2 cut(s) 153, 271
Aor13HI TCCGGA 1 cut(s) 260
AoxI GGCC 2 cut(s) 419, 429
ApaI GGGCCC 1 cut(s) 423
ApoI RAATTY 2 cut(s) 14, 404
AspS9I GGNCC 3 cut(s) 362, 419, 420
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 2 cut(s) 262, 310
AvaII GGWCC 1 cut(s) 362
BaeGI GKGCMC 2 cut(s) 286, 423
BanII GRGCYC 1 cut(s) 423
BccI CCATC 2 cut(s) 205, 285
BceAI ACGGC 2 cut(s) 406, 411
BciT130I CCWGG 1 cut(s) 457
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 1 cut(s) 278
BfaI CTAG 3 cut(s) 128, 305, 549
BglII AGATCT 1 cut(s) 37
BisI GCNGC 2 cut(s) 342, 380
BlsI GCNGC 2 cut(s) 343, 381
Bme1390I CCNGG 2 cut(s) 366, 457
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 3 cut(s) 362, 419, 420
BmiI GGNNCC 2 cut(s) 421, 422
BmrFI CCNGG 2 cut(s) 366, 457
BmsI GCATC 2 cut(s) 129, 307
BpuMI CCSGG 1 cut(s) 366
BsaWI WCCGGW 2 cut(s) 244, 260
Bse1I ACTGG 1 cut(s) 466
BseAI TCCGGA 1 cut(s) 260
BseBI CCWGG 1 cut(s) 457
BseGI GGATG 4 cut(s) 79, 216, 220, 298
BseNI ACTGG 1 cut(s) 466
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 2 cut(s) 286, 423
Bsh1236I CGCG 1 cut(s) 379
BshFI GGCC 2 cut(s) 421, 431
BsiSI CCGG 4 cut(s) 235, 245, 261, 365
BsmAI GTCTC 1 cut(s) 278
BsmBI CGTCTC 1 cut(s) 278
BsmI GAATGC 2 cut(s) 112, 122
BsnI GGCC 2 cut(s) 421, 431
Bsp120I GGGCCC 1 cut(s) 419
Bsp1286I GDGCHC 2 cut(s) 286, 423
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 4 cut(s) 37, 91, 145, 263
BspACI CCGC 5 cut(s) 164, 308, 341, 379, 424
BspANI GGCC 2 cut(s) 421, 431
BspEI TCCGGA 1 cut(s) 260
BspFNI CGCG 1 cut(s) 379
BspLI GGNNCC 2 cut(s) 421, 422
BspPI GGATC 2 cut(s) 153, 271
BsrI ACTGG 1 cut(s) 466
BssMI GATC 4 cut(s) 37, 91, 145, 263
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 334
BstF5I GGATG 4 cut(s) 79, 216, 220, 298
BstFNI CGCG 1 cut(s) 379
BstKTI GATC 4 cut(s) 40, 94, 148, 266
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 4 cut(s) 37, 91, 145, 263
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 1 cut(s) 457
BstSCI CCNGG 2 cut(s) 364, 455
BstSLI GKGCMC 2 cut(s) 286, 423
BstUI CGCG 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 421, 431
BtsCI GGATG 4 cut(s) 79, 216, 220, 298
Cac8I GCNNGC 1 cut(s) 334
Cfr13I GGNCC 3 cut(s) 362, 419, 420
CviJI RGCY 7 cut(s) 382, 398, 421, 431, 486, 502, 535
CviKI_1 RGCY 7 cut(s) 382, 398, 421, 431, 486, 502, 535
DpnI GATC 4 cut(s) 39, 93, 147, 265
DpnII GATC 4 cut(s) 37, 91, 145, 263
DraIII CACNNNGTG 1 cut(s) 324
DrdI GACNNNNNNGTC 1 cut(s) 466
DseDI GACNNNNNNGTC 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 455
EcoT22I ATGCAT 1 cut(s) 122
EcoT38I GRGCYC 1 cut(s) 423
Esp3I CGTCTC 1 cut(s) 278
FaiI YATR 2 cut(s) 475, 542
FauI CCCGC 1 cut(s) 431
Fnu4HI GCNGC 2 cut(s) 342, 380
FokI GGATG 4 cut(s) 86, 223, 227, 285
FriOI GRGCYC 1 cut(s) 423
Fsp4HI GCNGC 2 cut(s) 342, 380
FspBI CTAG 3 cut(s) 128, 305, 549
GluI GCNGC 2 cut(s) 342, 380
HaeIII GGCC 2 cut(s) 421, 431
HapII CCGG 4 cut(s) 235, 245, 261, 365
HincII GTYRAC 2 cut(s) 201, 471
HindII GTYRAC 2 cut(s) 201, 471
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 1 cut(s) 268
HpaII CCGG 4 cut(s) 235, 245, 261, 365
HphI GGTGA 2 cut(s) 262, 310
Hpy166II GTNNAC 2 cut(s) 201, 471
Hpy188I TCNGA 2 cut(s) 42, 372
Hpy188III TCNNGA 3 cut(s) 23, 53, 261
Hpy8I GTNNAC 2 cut(s) 201, 471
Hpy99I CGWCG 1 cut(s) 392
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpySE526I ACGT 1 cut(s) 181
Kpn2I TCCGGA 1 cut(s) 260
Kzo9I GATC 4 cut(s) 37, 91, 145, 263
LpnPI CCDG 8 cut(s) 36, 248, 258, 274, 378, 442, 447, 469
LweI GCATC 2 cut(s) 129, 307
MaeI CTAG 3 cut(s) 128, 305, 549
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 1 cut(s) 217
MalI GATC 4 cut(s) 39, 93, 147, 265
MboI GATC 4 cut(s) 37, 91, 145, 263
MboII GAAGA 4 cut(s) 47, 68, 152, 401
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 286, 423
MluCI AATT 3 cut(s) 14, 61, 404
MlyI GAGTC 1 cut(s) 262
MmeI TCCRAC 1 cut(s) 350
MnlI CCTC 8 cut(s) 30, 124, 166, 184, 187, 296, 312, 407
Mph1103I ATGCAT 1 cut(s) 122
MroI TCCGGA 1 cut(s) 260
MseI TTAA 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 4 cut(s) 235, 245, 261, 365
MspR9I CCNGG 2 cut(s) 366, 457
Mva1269I GAATGC 2 cut(s) 112, 122
MvaI CCWGG 1 cut(s) 457
MvnI CGCG 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 338
NciI CCSGG 1 cut(s) 366
NdeII GATC 4 cut(s) 37, 91, 145, 263
NlaIV GGNNCC 2 cut(s) 421, 422
NmuCI GTSAC 1 cut(s) 217
NsiI ATGCAT 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 277
PctI GAATGC 2 cut(s) 112, 122
PflFI GACNNNGTC 1 cut(s) 366
PfoI TCCNGGA 1 cut(s) 455
PkrI GCNGC 2 cut(s) 343, 381
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 455
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 2 cut(s) 421, 422
PspOMI GGGCCC 1 cut(s) 419
PspPI GGNCC 3 cut(s) 362, 419, 420
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 2 cut(s) 342, 380
Sau3AI GATC 4 cut(s) 37, 91, 145, 263
Sau96I GGNCC 3 cut(s) 362, 419, 420
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 2 cut(s) 366, 457
SduI GDGCHC 2 cut(s) 286, 423
SetI ASST 7 cut(s) 129, 135, 154, 184, 418, 504, 537
SfaNI GCATC 2 cut(s) 129, 307
SinI GGWCC 1 cut(s) 362
Sse9I AATT 3 cut(s) 14, 61, 404
SsiI CCGC 5 cut(s) 164, 308, 341, 379, 424
SspMI CTAG 3 cut(s) 128, 305, 549
StyD4I CCNGG 2 cut(s) 364, 455
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 1 cut(s) 184
TaqI TCGA 3 cut(s) 9, 266, 412
TasI AATT 3 cut(s) 14, 61, 404
TauI GCSGC 2 cut(s) 344, 382
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TseFI GTSAC 1 cut(s) 217
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 27, 129
Tth111I GACNNNGTC 1 cut(s) 366
VpaK11BI GGWCC 1 cut(s) 362
XapI RAATTY 2 cut(s) 14, 404
XspI CTAG 3 cut(s) 128, 305, 549
Zsp2I ATGCAT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.