Rorug05G0325800
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0017470
Physical Location & Seq
Reverse (-)
39021093 .. 39028404
7312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0325800.1

Sequence Viewer

Length: 1398 bp
ATGAAGCGGGGGTTGAGCTTGGACCAGGTGCTCAAGGACAACAACACTCGCGACCCCAACTCCATTTCTTCTCTCATCCTCACTCACAGGGCTCTCTCCGATGTTTCGTGCTTGGGCGAGCTCAAGAATCTGGAGAGGCTCGATCTCAGCGCCAACACGCTCACTTCGCTTGAGGGGTTGAAGTCATGCGTCAATCTGAAATGGCTGTCTGTTTCTCAAAATAAACTGCAAAGCCTCAAGGGAATTGAAGGACTTTCTAAGCTCACTGTGTTAAATGCAGGCAACAATAAGCTTACAGTGCTGGATGAAGTCAAGTCTATTGTGAGTTTGCGTGCATTAATTTTGAATGATAATGAGATTGCTTCGATTTGCAGACTTGAACAGATGAAAGAGTTGAACACTCTTGTTCTTTCTAAAAATCCGATTCGTGATATTGGTGATTCTTTGATGAATGTCAAATCTATCAAAAAGCTTTCTCTTTCTCACTGCCAACTTCGGAGTATACAGTCTTCGCTCAAGTCATGTGTTGAACTGAAAGAGCTGCGGCTCGCTCACAATAATATTGAGGGTCTCCCTGCTGAGTTGGCTCATAATAAGGGACTCCAGAATCTGGATCTGGGGAATAATGTGATCACAAGATGGTCGGAGCTGAAGGTGCTTGATTCCTTGGTCTATCTGAAGAATCTCAATCTGCAAGGAAACCCTGTAGCTGAAAAGGATAAATTAGCAAAGAAGATTAAAAAGATGTTGCCGAACCTGCATGTTTTCAATGCAAGACCAATAGATAAATACACCAAGAATGAGAAGGGTGCCCGGGTTGATGCAGTTGATGAAGAAAAATATAGAGATTCAAAACATCAAGGGTTGGGTGAAACGAAGGATAGTAACCTTGATAATGCTAGAGAATTGGATATGAAGAGGAAACGGAATAGGGAGAAAGCAAATGAGAATGTCTCGGACCAGGAAGTTTTGGTCCAGGAGGATGAAGAAGGGGATAGCATCAAGAAAAAGAAGAGACATCAGGTTGTAAATGAAGATTTTAGTAATCTTGAAGATGCTGCTGATTTGGAGGAATCAAAGGGAAAGAAACTGAAAAAGAAAGGCCATAAAGATGATGATACGAAGGTTAAAAAGAAACTAAATAAGGTGAAGCAACAAAGTGAACTTGATATTATTGATGACGCAGACACCCCATTTTTGGAGCTTTTTTCTGATGACAAAGTGGTAGATGCTGAGGATGATGGCGAACAAGGTAAGGACAAGGCTAGTCACACAAAATTGTCGAGTGGTGTAGTGACCATCACTTCAAGTAAAGCTAAGAAGCGGAGCACAGGTTCTACTTTACCGTTGTTGCCTGTAGATGAAATTGGAGTGGGTGGGCCATCAACATGGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

465

Amino Acids

51.81

Weight (kDa)

8.49

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 154 - 192 7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 177 - 235 1.6e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35540
pyrus_communis pycom111g03670
rosa_laevigata RLG00000002268 RLG00000020968
rosa_multiflora Rmu_sc0006282.1_g000017 Rmu_sc0008094.1_g000004
rosa_roxburghii Rroxscaffold_6G00393390
rosa_rugosa Rorug05G0325700 Rorug05G0325800 Rorug05G0325800
rosa_samantha Rh1AG000700 Rh1DG000500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 765
AccB1I GGYRCC 1 cut(s) 809
AccB7I CCANNNNNTGG 1 cut(s) 610
AccI GTMKAC 1 cut(s) 502
AccII CGCG 1 cut(s) 51
AciI CCGC 3 cut(s) 7, 544, 1324
AclWI GGATC 1 cut(s) 621
AcuI CTGAAG 2 cut(s) 671, 698
AfiI CCNNNNNNNGG 2 cut(s) 610, 1198
AjnI CCWGG 3 cut(s) 24, 960, 975
AloI GAACNNNNNNTCC 2 cut(s) 1318, 1350
Alw21I GWGCWC 3 cut(s) 33, 123, 1331
Alw26I GTCTC 3 cut(s) 575, 958, 1009
AlwI GGATC 1 cut(s) 621
AlwNI CAGNNNCTG 1 cut(s) 610
Ama87I CYCGRG 1 cut(s) 813
AoxI GGCC 2 cut(s) 1102, 1379
ApeKI GCWGC 2 cut(s) 541, 1058
AseI ATTAAT 1 cut(s) 338
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 4 cut(s) 22, 958, 973, 1379
AsuC2I CCSGG 2 cut(s) 814, 815
AsuHPI GGTGA 3 cut(s) 449, 881, 1159
AvaI CYCGRG 1 cut(s) 813
AvaII GGWCC 3 cut(s) 22, 958, 973
BaeGI GKGCMC 1 cut(s) 814
BaeI ACNNNNGTAYC 2 cut(s) 1110, 1143
BanI GGYRCC 1 cut(s) 809
BanII GRGCYC 2 cut(s) 94, 123
BarI GAAGNNNNNNTAC 2 cut(s) 493, 525
BbsI GAAGAC 1 cut(s) 501
Bbv12I GWGCWC 3 cut(s) 33, 123, 1331
BbvCI CCTCAGC 1 cut(s) 1233
BbvI GCAGC 2 cut(s) 528, 1045
BccI CCATC 4 cut(s) 633, 1235, 1307, 1390
BciT130I CCWGG 3 cut(s) 26, 962, 977
BclI TGATCA 1 cut(s) 630
BcnI CCSGG 2 cut(s) 814, 815
BcoDI GTCTC 3 cut(s) 575, 958, 1009
BfaI CTAG 2 cut(s) 900, 1266
BfmI CTRYAG 2 cut(s) 705, 1356
BfoI RGCGCY 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 765
BisI GCNGC 3 cut(s) 542, 545, 1059
BlsI GCNGC 3 cut(s) 543, 546, 1060
Bme1390I CCNGG 5 cut(s) 26, 814, 815, 962, 977
Bme18I GGWCC 3 cut(s) 22, 958, 973
BmeT110I CYCGRG 1 cut(s) 813
BmgT120I GGNCC 4 cut(s) 22, 958, 973, 1379
BmiI GGNNCC 1 cut(s) 811
BmrFI CCNGG 5 cut(s) 26, 814, 815, 962, 977
BmsI GCATC 4 cut(s) 811, 1008, 1045, 1219
BpiI GAAGAC 1 cut(s) 501
BplI GAGNNNNNCTC 2 cut(s) 938, 970
BpmI CTGGAG 2 cut(s) 152, 587
Bpu10I CCTNAGC 1 cut(s) 1233
BpuEI CTTGAG 5 cut(s) 17, 107, 191, 221, 500
BpuMI CCSGG 2 cut(s) 814, 815
BsaI GGTCTC 1 cut(s) 575
BsaJI CCNNGG 2 cut(s) 666, 813
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 1318, 1348
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 1198
BseBI CCWGG 3 cut(s) 26, 962, 977
BseDI CCNNGG 2 cut(s) 666, 813
BseGI GGATG 4 cut(s) 75, 310, 988, 1243
BseLI CCNNNNNNNGG 2 cut(s) 610, 1198
BseMII CTCAG 3 cut(s) 160, 570, 1224
BseSI GKGCMC 1 cut(s) 814
BseXI GCAGC 2 cut(s) 528, 1045
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 2 cut(s) 1104, 1381
BshNI GGYRCC 1 cut(s) 809
BsiHKAI GWGCWC 3 cut(s) 33, 123, 1331
BsiHKCI CYCGRG 1 cut(s) 813
BsiSI CCGG 1 cut(s) 814
BslFI GGGAC 1 cut(s) 612
BslI CCNNNNNNNGG 2 cut(s) 610, 1198
BsmAI GTCTC 3 cut(s) 575, 958, 1009
BsmFI GGGAC 1 cut(s) 612
BsnI GGCC 2 cut(s) 1104, 1381
Bso31I GGTCTC 1 cut(s) 575
BsoBI CYCGRG 1 cut(s) 813
Bsp1286I GDGCHC 5 cut(s) 33, 94, 123, 814, 1331
Bsp143I GATC 3 cut(s) 142, 613, 630
Bsp68I TCGCGA 1 cut(s) 51
BspACI CCGC 3 cut(s) 7, 544, 1324
BspANI GGCC 2 cut(s) 1104, 1381
BspCNI CTCAG 3 cut(s) 159, 571, 1225
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 811
BspMI ACCTGC 1 cut(s) 765
BspPI GGATC 1 cut(s) 621
BspT107I GGYRCC 1 cut(s) 809
BspTNI GGTCTC 1 cut(s) 575
BssECI CCNNGG 2 cut(s) 666, 813
BssMI GATC 3 cut(s) 142, 613, 630
BssNAI GTATAC 1 cut(s) 503
BssT1I CCWWGG 1 cut(s) 666
Bst1107I GTATAC 1 cut(s) 503
Bst2UI CCWGG 3 cut(s) 26, 962, 977
Bst4CI ACNGT 4 cut(s) 268, 298, 507, 1347
Bst6I CTCTTC 2 cut(s) 911, 1007
BstC8I GCNNGC 4 cut(s) 119, 280, 333, 549
BstDEI CTNAG 5 cut(s) 146, 258, 579, 1233, 1317
BstF5I GGATG 4 cut(s) 75, 310, 988, 1243
BstFNI CGCG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 3 cut(s) 145, 616, 633
BstMAI GTCTC 3 cut(s) 575, 958, 1009
BstMBI GATC 3 cut(s) 142, 613, 630
BstMWI GCNNNNNNNGC 5 cut(s) 166, 298, 584, 655, 757
BstNI CCWGG 3 cut(s) 26, 962, 977
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 5 cut(s) 24, 812, 813, 960, 975
BstSFI CTRYAG 2 cut(s) 705, 1356
BstSLI GKGCMC 1 cut(s) 814
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 528, 1045
BstV2I GAAGAC 1 cut(s) 501
BstX2I RGATCY 1 cut(s) 613
BstXI CCANNNNNNTGG 1 cut(s) 1389
BstYI RGATCY 1 cut(s) 613
BstZ17I GTATAC 1 cut(s) 503
BsuRI GGCC 2 cut(s) 1104, 1381
BtsCI GGATG 4 cut(s) 75, 310, 988, 1243
BtsI GCAGTG 1 cut(s) 484
BtsIMutI CAGTG 3 cut(s) 264, 303, 484
BtuMI TCGCGA 1 cut(s) 51
BveI ACCTGC 1 cut(s) 765
Cac8I GCNNGC 4 cut(s) 119, 280, 333, 549
CaiI CAGNNNCTG 1 cut(s) 610
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 4 cut(s) 22, 958, 973, 1379
Cfr9I CCCGGG 1 cut(s) 813
CseI GACGC 2 cut(s) 178, 1190
CsiI ACCWGGT 1 cut(s) 24
CviAII CATG 4 cut(s) 186, 522, 761, 1389
DdeI CTNAG 5 cut(s) 146, 258, 579, 1233, 1317
DpnI GATC 3 cut(s) 144, 615, 632
DpnII GATC 3 cut(s) 142, 613, 630
Eam1104I CTCTTC 2 cut(s) 911, 1007
EarI CTCTTC 2 cut(s) 911, 1007
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 666
Eco24I GRGCYC 2 cut(s) 94, 123
Eco31I GGTCTC 1 cut(s) 575
Eco47I GGWCC 3 cut(s) 22, 958, 973
Eco53kI GAGCTC 1 cut(s) 121
Eco57I CTGAAG 2 cut(s) 671, 698
Eco88I CYCGRG 1 cut(s) 813
EcoICRI GAGCTC 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 24, 960, 975
EcoT14I CCWWGG 1 cut(s) 666
EcoT38I GRGCYC 2 cut(s) 94, 123
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 4 cut(s) 189, 525, 764, 1392
FaiI YATR 9 cut(s) 187, 503, 523, 591, 762, 843, 914, 1107, 1390
FaqI GGGAC 1 cut(s) 612
FatI CATG 4 cut(s) 185, 521, 760, 1388
FbaI TGATCA 1 cut(s) 630
FblI GTMKAC 1 cut(s) 502
Fnu4HI GCNGC 3 cut(s) 542, 545, 1059
FokI GGATG 4 cut(s) 62, 317, 995, 1250
FriOI GRGCYC 2 cut(s) 94, 123
Fsp4HI GCNGC 3 cut(s) 542, 545, 1059
FspBI CTAG 2 cut(s) 900, 1266
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 542, 545, 1059
GsuI CTGGAG 2 cut(s) 152, 587
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 2 cut(s) 1104, 1381
HapII CCGG 1 cut(s) 814
HgaI GACGC 2 cut(s) 178, 1190
HhaI GCGC 1 cut(s) 152
Hin1II CATG 4 cut(s) 189, 525, 764, 1392
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HindIII AAGCTT 2 cut(s) 290, 470
HinfI GANTC 9 cut(s) 127, 424, 440, 600, 607, 662, 682, 848, 1073
HpaII CCGG 1 cut(s) 814
HphI GGTGA 3 cut(s) 449, 881, 1159
Hpy166II GTNNAC 2 cut(s) 503, 1163
Hpy188I TCNGA 8 cut(s) 100, 198, 423, 498, 646, 678, 958, 1213
Hpy188III TCNNGA 8 cut(s) 50, 124, 131, 428, 604, 611, 1003, 1049
Hpy8I GTNNAC 2 cut(s) 503, 1163
HpyAV CCTTC 6 cut(s) 242, 646, 799, 871, 983, 1117
HpyCH4III ACNGT 4 cut(s) 268, 298, 507, 1347
HpyCH4V TGCA 8 cut(s) 229, 278, 335, 372, 694, 760, 773, 824
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 298, 584, 655, 757
HpyF3I CTNAG 5 cut(s) 146, 258, 579, 1233, 1317
Hsp92II CATG 4 cut(s) 189, 525, 764, 1392
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 630
Kzo9I GATC 3 cut(s) 142, 613, 630
LmnI GCTCC 3 cut(s) 646, 1201, 1326
Lsp1109I GCAGC 2 cut(s) 528, 1045
LweI GCATC 4 cut(s) 811, 1008, 1045, 1219
MabI ACCWGGT 1 cut(s) 24
MaeI CTAG 2 cut(s) 900, 1266
MaeIII GTNAC 3 cut(s) 884, 1268, 1294
MalI GATC 3 cut(s) 144, 615, 632
MboI GATC 3 cut(s) 142, 613, 630
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 5 cut(s) 33, 94, 123, 814, 1331
MluCI AATT 6 cut(s) 243, 339, 722, 905, 1277, 1365
MlyI GAGTC 1 cut(s) 594
MmeI TCCRAC 1 cut(s) 624
MnlI CCTC 9 cut(s) 89, 129, 166, 245, 559, 912, 973, 1063, 1228
MseI TTAA 4 cut(s) 272, 338, 738, 1128
MslI CAYNNNNRTG 2 cut(s) 1110, 1387
MspI CCGG 1 cut(s) 814
MspR9I CCNGG 5 cut(s) 26, 814, 815, 962, 977
MvaI CCWGG 3 cut(s) 26, 962, 977
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 5 cut(s) 166, 298, 584, 655, 757
NciI CCSGG 2 cut(s) 814, 815
NdeII GATC 3 cut(s) 142, 613, 630
NlaIII CATG 4 cut(s) 189, 525, 764, 1392
NlaIV GGNNCC 1 cut(s) 811
NmuCI GTSAC 2 cut(s) 1268, 1294
NruI TCGCGA 1 cut(s) 51
NspI RCATGY 1 cut(s) 764
PfeI GAWTC 8 cut(s) 127, 424, 440, 607, 662, 682, 848, 1073
PflMI CCANNNNNTGG 1 cut(s) 610
PfoI TCCNGGA 1 cut(s) 975
PkrI GCNGC 3 cut(s) 543, 546, 1060
PleI GAGTC 1 cut(s) 594
PpsI GAGTC 1 cut(s) 594
PshBI ATTAAT 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 123
Psp6I CCWGG 3 cut(s) 24, 960, 975
PspGI CCWGG 3 cut(s) 24, 960, 975
PspN4I GGNNCC 1 cut(s) 811
PspPI GGNCC 4 cut(s) 22, 958, 973, 1379
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 1 cut(s) 613
RruI TCGCGA 1 cut(s) 51
RseI CAYNNNNRTG 2 cut(s) 1110, 1387
SacI GAGCTC 1 cut(s) 123
SaqAI TTAA 4 cut(s) 272, 338, 738, 1128
SatI GCNGC 3 cut(s) 542, 545, 1059
Sau3AI GATC 3 cut(s) 142, 613, 630
Sau96I GGNCC 4 cut(s) 22, 958, 973, 1379
SchI GAGTC 1 cut(s) 594
ScrFI CCNGG 5 cut(s) 26, 814, 815, 962, 977
SduI GDGCHC 5 cut(s) 33, 94, 123, 814, 1331
SexAI ACCWGGT 1 cut(s) 24
SfaNI GCATC 4 cut(s) 811, 1008, 1045, 1219
SfcI CTRYAG 2 cut(s) 705, 1356
SinI GGWCC 3 cut(s) 22, 958, 973
SmaI CCCGGG 1 cut(s) 815
SmiMI CAYNNNNRTG 2 cut(s) 1110, 1387
SmlI CTYRAG 5 cut(s) 32, 122, 170, 236, 515
SmoI CTYRAG 5 cut(s) 32, 122, 170, 236, 515
Sse9I AATT 6 cut(s) 243, 339, 722, 905, 1277, 1365
SsiI CCGC 3 cut(s) 7, 544, 1324
SspI AATATT 1 cut(s) 562
SspMI CTAG 2 cut(s) 900, 1266
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 5 cut(s) 24, 812, 813, 960, 975
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 4 cut(s) 268, 298, 507, 1347
TaqI TCGA 3 cut(s) 141, 365, 1283
TasI AATT 6 cut(s) 243, 339, 722, 905, 1277, 1365
TauI GCSGC 1 cut(s) 547
TfiI GAWTC 8 cut(s) 127, 424, 440, 607, 662, 682, 848, 1073
Tru1I TTAA 4 cut(s) 272, 338, 738, 1128
Tru9I TTAA 4 cut(s) 272, 338, 738, 1128
TscAI CASTG 3 cut(s) 271, 303, 491
TseFI GTSAC 2 cut(s) 1268, 1294
TseI GCWGC 2 cut(s) 541, 1058
Tsp45I GTSAC 2 cut(s) 1268, 1294
TspDTI ATGAA 9 cut(s) 17, 321, 401, 464, 846, 929, 999, 1047, 1377
TspGWI ACGGA 1 cut(s) 940
TspMI CCCGGG 1 cut(s) 813
TspRI CASTG 3 cut(s) 271, 303, 491
Van91I CCANNNNNTGG 1 cut(s) 610
VpaK11BI GGWCC 3 cut(s) 22, 958, 973
VspI ATTAAT 1 cut(s) 338
XceI RCATGY 1 cut(s) 764
XmaI CCCGGG 1 cut(s) 813
XmiI GTMKAC 1 cut(s) 502
XspI CTAG 2 cut(s) 900, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.