Rh1AG000700
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0017470
Physical Location & Seq
Forward (+)
165217 .. 172227
7011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG000700.1

Sequence Viewer

Length: 645 bp
ATGTTAAGAGAGCCGACATTGAACAACAGTAAGAGAAGAAAGAAAATTGGTGAAGAAGGATACCTCGTCGGCCTGATGTTCATTAACAGCGACTGGGTCTCTTGGGCCATTACTGGGATGACCTGCGCATCTGTAGTTTCGATTAACTCTTTACTGAGCCCAGCCTCAGCGTGTTCTTTCCGTGCAAAATTCAAGTTCTTCTTACTCGACTCCAATCCACTTCTGAGTCTTTCTTGCCAGCCGACTGCCGACGCCGCTTCGTTCGGTCCAAAGTTTGACGACAATCACACTTTCTACTGGTGCATAAATGAATTTCATGGACGAGGATGCTTCTTCGGGCTCTGCTGCCGCTTCAAAAATGAGGGCGACCGCAATCATGAAGAACGAGGACCGGTTTTCTATAAGCAAATGCATAAAGATACATCAGCTGAAATGGATGATTTCGACTTAGAACTGGATGAGATGGAATTAACTGCAGCAGCGGCTGGCGTGGAAGGGTCAACAGTGGATGACCTACCTGATGTTGATGAACATCCTGATATTGGGTTGGTTTCTTCGGTACAAGAGCAGGTTGCGTTTTCAATGAGAGAATCAATTTCAGCAGAAATGTGGGATCACTTTATGAATGGATGGGATCAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

214

Amino Acids

24.18

Weight (kDa)

4.63

Isoelectric Point (pI)

30.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35540
pyrus_communis pycom111g03670
rosa_laevigata RLG00000002268 RLG00000020968
rosa_multiflora Rmu_sc0006282.1_g000017 Rmu_sc0008094.1_g000004
rosa_roxburghii Rroxscaffold_6G00393390
rosa_rugosa Rorug05G0325700 Rorug05G0325800 Rorug05G0325800
rosa_samantha Rh1AG000700 Rh1DG000500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 127
Acc36I ACCTGC 2 cut(s) 131, 559
AciI CCGC 4 cut(s) 255, 349, 370, 482
AclWI GGATC 2 cut(s) 621, 642
AcsI RAATTY 2 cut(s) 188, 311
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 1 cut(s) 561
AfiI CCNNNNNNNGG 2 cut(s) 114, 542
AgeI ACCGGT 1 cut(s) 391
AgsI TTSAA 4 cut(s) 22, 193, 355, 582
AluBI AGCT 1 cut(s) 428
AluI AGCT 1 cut(s) 428
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 2 cut(s) 621, 642
AlwNI CAGNNNCTG 2 cut(s) 93, 485
AoxI GGCC 2 cut(s) 70, 105
ApeKI GCWGC 3 cut(s) 345, 476, 479
ApoI RAATTY 2 cut(s) 188, 311
AsiGI ACCGGT 1 cut(s) 391
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 3 cut(s) 105, 266, 389
AsuHPI GGTGA 1 cut(s) 62
AvaII GGWCC 2 cut(s) 266, 389
BanII GRGCYC 2 cut(s) 161, 342
BbvCI CCTCAGC 1 cut(s) 166
BbvI GCAGC 3 cut(s) 332, 488, 491
BccI CCATC 2 cut(s) 457, 624
BciVI GTATCC 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 103
BfmI CTRYAG 2 cut(s) 132, 474
BfuAI ACCTGC 2 cut(s) 131, 559
BfuI GTATCC 1 cut(s) 53
BisI GCNGC 6 cut(s) 255, 346, 349, 477, 480, 483
BlsI GCNGC 6 cut(s) 256, 347, 350, 478, 481, 484
Bme18I GGWCC 2 cut(s) 266, 389
BmgT120I GGNCC 3 cut(s) 105, 266, 389
BmrI ACTGGG 2 cut(s) 103, 123
BmsI GCATC 2 cut(s) 137, 317
BmuI ACTGGG 2 cut(s) 103, 123
Bpu10I CCTNAGC 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 103
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 542
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 4 cut(s) 98, 118, 302, 459
Bse8I GATNNNNATC 1 cut(s) 531
BseGI GGATG 7 cut(s) 123, 332, 442, 463, 514, 532, 635
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 2 cut(s) 114, 542
BseMII CTCAG 3 cut(s) 146, 180, 215
BseNI ACTGG 4 cut(s) 98, 118, 302, 459
BseXI GCAGC 3 cut(s) 332, 488, 491
BseYI CCCAGC 1 cut(s) 160
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 2 cut(s) 72, 107
BshTI ACCGGT 1 cut(s) 391
BsiEI CGRYCG 1 cut(s) 370
BsiSI CCGG 1 cut(s) 392
BslI CCNNNNNNNGG 2 cut(s) 114, 542
BsmAI GTCTC 1 cut(s) 103
BsnI GGCC 2 cut(s) 72, 107
Bso31I GGTCTC 1 cut(s) 103
Bsp1286I GDGCHC 2 cut(s) 161, 342
Bsp143I GATC 2 cut(s) 613, 634
BspACI CCGC 4 cut(s) 255, 349, 370, 482
BspANI GGCC 2 cut(s) 72, 107
BspCNI CTCAG 3 cut(s) 147, 179, 216
BspHI TCATGA 1 cut(s) 376
BspMAI CTGCAG 1 cut(s) 478
BspMI ACCTGC 2 cut(s) 131, 559
BspPI GGATC 2 cut(s) 621, 642
BspTNI GGTCTC 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 4 cut(s) 98, 118, 302, 459
BssAI RCCGGY 1 cut(s) 391
BssMI GATC 2 cut(s) 613, 634
BssNI GRCGYC 1 cut(s) 252
Bst4CI ACNGT 2 cut(s) 29, 505
BstACI GRCGYC 1 cut(s) 252
BstC8I GCNNGC 2 cut(s) 239, 487
BstDEI CTNAG 4 cut(s) 155, 166, 224, 448
BstF5I GGATG 7 cut(s) 123, 332, 442, 463, 514, 532, 635
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 2 cut(s) 616, 637
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 2 cut(s) 613, 634
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 2 cut(s) 254, 482
BstSFI CTRYAG 2 cut(s) 132, 474
BstV1I GCAGC 3 cut(s) 332, 488, 491
BsuI GTATCC 1 cut(s) 53
BsuRI GGCC 2 cut(s) 72, 107
BtsCI GGATG 7 cut(s) 123, 332, 442, 463, 514, 532, 635
BtsIMutI CAGTG 2 cut(s) 510, 644
BveI ACCTGC 2 cut(s) 131, 559
Cac8I GCNNGC 2 cut(s) 239, 487
CaiI CAGNNNCTG 2 cut(s) 93, 485
CciI TCATGA 1 cut(s) 376
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 3 cut(s) 105, 266, 389
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 560
CspAI ACCGGT 1 cut(s) 391
CviAII CATG 2 cut(s) 317, 377
CviJI RGCY 9 cut(s) 13, 72, 107, 159, 164, 241, 340, 428, 485
CviKI_1 RGCY 9 cut(s) 13, 72, 107, 159, 164, 241, 340, 428, 485
CviQI GTAC 1 cut(s) 560
DdeI CTNAG 4 cut(s) 155, 166, 224, 448
DpnI GATC 2 cut(s) 615, 636
DpnII GATC 2 cut(s) 613, 634
Eco24I GRGCYC 2 cut(s) 161, 342
Eco31I GGTCTC 1 cut(s) 103
Eco47I GGWCC 2 cut(s) 266, 389
EcoT22I ATGCAT 1 cut(s) 414
EcoT38I GRGCYC 2 cut(s) 161, 342
FaeI CATG 2 cut(s) 320, 380
FaiI YATR 6 cut(s) 305, 318, 378, 402, 414, 623
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FatI CATG 2 cut(s) 316, 376
Fnu4HI GCNGC 6 cut(s) 255, 346, 349, 477, 480, 483
FokI GGATG 6 cut(s) 130, 339, 449, 470, 519, 521
FriOI GRGCYC 2 cut(s) 161, 342
Fsp4HI GCNGC 6 cut(s) 255, 346, 349, 477, 480, 483
FspI TGCGCA 1 cut(s) 127
GlaI GCGC 1 cut(s) 127
GluI GCNGC 6 cut(s) 255, 346, 349, 477, 480, 483
GsaI CCCAGC 1 cut(s) 164
HaeIII GGCC 2 cut(s) 72, 107
HapII CCGG 1 cut(s) 392
HgaI GACGC 1 cut(s) 260
HhaI GCGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 2 cut(s) 320, 380
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HinfI GANTC 3 cut(s) 209, 226, 590
HpaII CCGG 1 cut(s) 392
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 1 cut(s) 501
Hpy188I TCNGA 1 cut(s) 225
Hpy188III TCNNGA 2 cut(s) 377, 536
Hpy8I GTNNAC 1 cut(s) 501
Hpy99I CGWCG 2 cut(s) 71, 254
HpyAV CCTTC 2 cut(s) 50, 488
HpyCH4III ACNGT 2 cut(s) 29, 505
HpyCH4V TGCA 4 cut(s) 185, 303, 412, 476
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 482
HpyF3I CTNAG 4 cut(s) 155, 166, 224, 448
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 2 cut(s) 320, 380
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 2 cut(s) 613, 634
Lsp1109I GCAGC 3 cut(s) 332, 488, 491
LweI GCATC 2 cut(s) 137, 317
MalI GATC 2 cut(s) 615, 636
MboI GATC 2 cut(s) 613, 634
MboII GAAGA 6 cut(s) 48, 65, 190, 325, 392, 546
MhlI GDGCHC 2 cut(s) 161, 342
MluCI AATT 5 cut(s) 45, 188, 311, 467, 594
MlyI GAGTC 2 cut(s) 203, 235
MnlI CCTC 5 cut(s) 74, 175, 317, 355, 380
Mph1103I ATGCAT 1 cut(s) 414
MseI TTAA 4 cut(s) 5, 84, 144, 470
MspA1I CMGCKG 2 cut(s) 428, 482
MspI CCGG 1 cut(s) 392
MwoI GCNNNNNNNGC 2 cut(s) 254, 482
NdeII GATC 2 cut(s) 613, 634
NlaIII CATG 2 cut(s) 320, 380
NsbI TGCGCA 1 cut(s) 127
NsiI ATGCAT 1 cut(s) 414
PagI TCATGA 1 cut(s) 376
PfeI GAWTC 1 cut(s) 590
PflFI GACNNNGTC 1 cut(s) 95
PinAI ACCGGT 1 cut(s) 391
PkrI GCNGC 6 cut(s) 256, 347, 350, 478, 481, 484
PleI GAGTC 2 cut(s) 203, 234
PpsI GAGTC 2 cut(s) 203, 234
PspFI CCCAGC 1 cut(s) 160
PspPI GGNCC 3 cut(s) 105, 266, 389
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 2 cut(s) 93, 485
PsyI GACNNNGTC 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
SaqAI TTAA 4 cut(s) 5, 84, 144, 470
SatI GCNGC 6 cut(s) 255, 346, 349, 477, 480, 483
Sau3AI GATC 2 cut(s) 613, 634
Sau96I GGNCC 3 cut(s) 105, 266, 389
SchI GAGTC 2 cut(s) 203, 235
SduI GDGCHC 2 cut(s) 161, 342
SetI ASST 6 cut(s) 66, 125, 430, 516, 520, 573
SfaNI GCATC 2 cut(s) 137, 317
SfcI CTRYAG 2 cut(s) 132, 474
SinI GGWCC 2 cut(s) 266, 389
Sse9I AATT 5 cut(s) 45, 188, 311, 467, 594
SsiI CCGC 4 cut(s) 255, 349, 370, 482
TaaI ACNGT 2 cut(s) 29, 505
TaqI TCGA 3 cut(s) 140, 207, 444
TaqII GACCGA 1 cut(s) 254
TasI AATT 5 cut(s) 45, 188, 311, 467, 594
TauI GCSGC 3 cut(s) 257, 351, 485
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 4 cut(s) 5, 84, 144, 470
Tru9I TTAA 4 cut(s) 5, 84, 144, 470
TscAI CASTG 2 cut(s) 510, 644
TseI GCWGC 3 cut(s) 345, 476, 479
TspDTI ATGAA 6 cut(s) 70, 305, 324, 393, 543, 638
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 2 cut(s) 510, 644
Tth111I GACNNNGTC 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 266, 389
XapI RAATTY 2 cut(s) 188, 311
Zsp2I ATGCAT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.