RLG00000020968
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017470
Physical Location & Seq
Reverse (-)
70578376 .. 70578771
396 bp
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UTR
Exon/CDS
Intron
RLM00000020968

Sequence Viewer

Length: 396 bp
ATGGATCCTTGTCGTAAGCGTGCTAGAGAAGAAGATGAGGAGGAGGAAGAGGTGGTGAAGGATGAAGAAAGAATAGGGAAAATGAAAGCCTTTTTGGCTGCTGTAACTGGATTTATAGCAGCAATAGTAGCTTGGTACCATTGTAAGTTACTCACAAGAAAAGAGCCATTGCCTGATTGGGATGAGCATAGACGATTCTATTTGAATCGATTAAGTGGATCTAAAGTTGAGTGTATGAAAAACTTAAGAGTTAGCATGTATGGATTTGCAAAACTTTGCCAAGTCTTATACGACAAAGGTGGATTACGAAGAACAAGGCATGTATCTATTAAAGAATATGTATTTCGCGAAATCAACGAAGAGGAGATTAGAACTGTTGAAGCCACTAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

132

Amino Acids

15.54

Weight (kDa)

8.33

Isoelectric Point (pI)

59.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 69 - 114 2.3e-07 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35540
pyrus_communis pycom111g03670
rosa_laevigata RLG00000002268 RLG00000020968
rosa_multiflora Rmu_sc0006282.1_g000017 Rmu_sc0008094.1_g000004
rosa_roxburghii Rroxscaffold_6G00393390
rosa_rugosa Rorug05G0325700 Rorug05G0325800 Rorug05G0325800
rosa_samantha Rh1AG000700 Rh1DG000500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 135
AccII CGCG 1 cut(s) 348
AclWI GGATC 2 cut(s) 12, 226
AfaI GTAC 1 cut(s) 137
AflII CTTAAG 1 cut(s) 244
AgsI TTSAA 2 cut(s) 205, 380
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 1 cut(s) 131
AluI AGCT 1 cut(s) 131
AlwI GGATC 2 cut(s) 12, 226
ApeKI GCWGC 2 cut(s) 98, 119
Asp718I GGTACC 1 cut(s) 135
AsuHPI GGTGA 1 cut(s) 67
BaeI ACNNNNGTAYC 2 cut(s) 127, 160
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 85, 131
BfaI CTAG 1 cut(s) 24
BfrI CTTAAG 1 cut(s) 244
BglI GCCNNNNNGGC 1 cut(s) 95
BisI GCNGC 2 cut(s) 99, 120
BlsI GCNGC 2 cut(s) 100, 121
BmiI GGNNCC 2 cut(s) 6, 137
Bsa29I ATCGAT 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 167
BseCI ATCGAT 1 cut(s) 208
BseGI GGATG 2 cut(s) 67, 187
BseMI GCAATG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 112
BseRI GAGGAG 3 cut(s) 53, 56, 377
BseXI GCAGC 2 cut(s) 85, 131
Bsh1236I CGCG 1 cut(s) 348
BshNI GGYRCC 1 cut(s) 135
BshVI ATCGAT 1 cut(s) 208
Bsp143I GATC 2 cut(s) 4, 218
Bsp68I TCGCGA 1 cut(s) 348
BspDI ATCGAT 1 cut(s) 208
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 6, 137
BspPI GGATC 2 cut(s) 12, 226
BspT107I GGYRCC 1 cut(s) 135
BspTI CTTAAG 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 167
BsrI ACTGG 1 cut(s) 112
BssMI GATC 2 cut(s) 4, 218
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 2 cut(s) 42, 354
BstAFI CTTAAG 1 cut(s) 244
BstC8I GCNNGC 1 cut(s) 21
BstF5I GGATG 2 cut(s) 67, 187
BstFNI CGCG 1 cut(s) 348
BstKTI GATC 2 cut(s) 7, 221
BstMBI GATC 2 cut(s) 4, 218
BstMWI GCNNNNNNNGC 2 cut(s) 95, 128
BstNSI RCATGY 2 cut(s) 259, 323
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 85, 131
BstX2I RGATCY 2 cut(s) 4, 218
BstYI RGATCY 2 cut(s) 4, 218
Bsu15I ATCGAT 1 cut(s) 208
BsuTUI ATCGAT 1 cut(s) 208
BtsCI GGATG 2 cut(s) 67, 187
BtuMI TCGCGA 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 21
ClaI ATCGAT 1 cut(s) 208
Csp6I GTAC 1 cut(s) 136
CviAII CATG 2 cut(s) 256, 320
CviJI RGCY 5 cut(s) 89, 98, 131, 166, 383
CviKI_1 RGCY 5 cut(s) 89, 98, 131, 166, 383
CviQI GTAC 1 cut(s) 136
DpnI GATC 2 cut(s) 6, 220
DpnII GATC 2 cut(s) 4, 218
Eam1104I CTCTTC 2 cut(s) 42, 354
EarI CTCTTC 2 cut(s) 42, 354
FaeI CATG 2 cut(s) 259, 323
FaiI YATR 8 cut(s) 116, 189, 236, 257, 261, 289, 321, 339
FatI CATG 2 cut(s) 255, 319
Fnu4HI GCNGC 2 cut(s) 99, 120
FokI GGATG 2 cut(s) 74, 194
Fsp4HI GCNGC 2 cut(s) 99, 120
FspBI CTAG 1 cut(s) 24
GluI GCNGC 2 cut(s) 99, 120
Hin1II CATG 2 cut(s) 259, 323
HinfI GANTC 2 cut(s) 195, 205
HphI GGTGA 1 cut(s) 67
Hpy188III TCNNGA 1 cut(s) 347
HpyAV CCTTC 1 cut(s) 52
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 128
Hsp92II CATG 2 cut(s) 259, 323
KpnI GGTACC 1 cut(s) 139
Kzo9I GATC 2 cut(s) 4, 218
LpnPI CCDG 2 cut(s) 93, 186
Lsp1109I GCAGC 2 cut(s) 85, 131
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 103, 147
MalI GATC 2 cut(s) 6, 220
MboI GATC 2 cut(s) 4, 218
MboII GAAGA 6 cut(s) 41, 44, 59, 77, 321, 371
MflI RGATCY 2 cut(s) 4, 218
MnlI CCTC 5 cut(s) 31, 34, 37, 43, 355
MseI TTAA 3 cut(s) 212, 245, 330
MspCI CTTAAG 1 cut(s) 244
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 2 cut(s) 95, 128
NdeII GATC 2 cut(s) 4, 218
NlaIII CATG 2 cut(s) 259, 323
NlaIV GGNNCC 2 cut(s) 6, 137
NruI TCGCGA 1 cut(s) 348
NspI RCATGY 2 cut(s) 259, 323
PfeI GAWTC 2 cut(s) 195, 205
PkrI GCNGC 2 cut(s) 100, 121
PspN4I GGNNCC 2 cut(s) 6, 137
PsuI RGATCY 2 cut(s) 4, 218
RruI TCGCGA 1 cut(s) 348
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
SaqAI TTAA 3 cut(s) 212, 245, 330
SatI GCNGC 2 cut(s) 99, 120
Sau3AI GATC 2 cut(s) 4, 218
SetI ASST 3 cut(s) 54, 133, 301
SmlI CTYRAG 1 cut(s) 244
SmoI CTYRAG 1 cut(s) 244
SspMI CTAG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 376
TaqI TCGA 1 cut(s) 208
TfiI GAWTC 2 cut(s) 195, 205
Tru1I TTAA 3 cut(s) 212, 245, 330
Tru9I TTAA 3 cut(s) 212, 245, 330
TseI GCWGC 2 cut(s) 98, 119
TspDTI ATGAA 3 cut(s) 78, 98, 251
Vha464I CTTAAG 1 cut(s) 244
XceI RCATGY 2 cut(s) 259, 323
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.