RLG00000007885

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017142
Physical Location & Seq
Forward (+)
22423188 .. 22424438
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007885

Sequence Viewer

Length: 1251 bp
ATGGCTGCTTTTCTGCACTTCCTTCTTCTTCTCTGCTCTTTCGCCTACAATTTCAACACATCTCAATCCAATCATACTTACCCAGAACCAAAAGTTGCCATCTTTCACGTCACCAAAGACCTATCAACCCTCCAGTACGTAACCCAGATCCACCACGGCTCTCCACTCCAGCCCACCAACCTCGTCATCGATCTCGGCGGCCCATTCCTCTGGCTCGGCTGCAATTCCGATTCCGGGTCTTCATCTTCTAGAACTCTCATCTCTCATAGCTCAATCAAATGCTTATCAGCAAACCCAGACACCCAAAAAAGCAAGACCAGCAAGTGTGAGACTTTCCCTGAAAACCGATTCACCCAAATGGCCACACAAGGGGATTTGGCACAAGACATCATAGCCCTTCCGTTCGTTGACGAGTCCAAAACAGAGGCCATCACCGCTGTTAATTCCTATTTTTTATTTTCATGTGCACCCAAATTGCTTCTGAAAGGCCTAGCAACTGGGGCTGGAGGAATGTTAGGACTCGGAAGGAGTCGAGTTTCGGTGGCGTCACAAATTGCTGCAGCATTTGGCTCCAAACCGCAGTTCATCCTCTGCCTGTCTTCATCAAACGGGGTCGTTTTACACGACAGTGCGGAATATGGTTCTTATTTTGTCTCTGGATTTTCAGGCTCACTCACATACACACCCCTGGTTACTAAAGTTGACTCACCACATGAGTATTTCATCAACCTGAAGTCAATCAGAGTCAACGGGAAGCAGTTGTCTCTGAATTACAAAGAGGGTCTTGGAGGGATTAAGCTAAGCACAGTTGTTCCCTACTCTACTTTGGAAAGCTCAGTCTATGCTGTCTTTGTCAAAGCTTATGAGCAAGCTGCTGTGGCTATGAACATGACTAGGGTGGATTCTGTGGCACCATTTGGACTGTGTTTTGGTTCCAAAAACATCGATGGCATTCGGGTTGGTTCAAGAGCGCCTGTTGTTGATTTGGGGCTGCAGAGTGAGATGGTGAAGTGGAGAATTCATGGTAGAAACTCCGTGATTCAAGTGAGCGATGAAGTTATGTGTTTGGGGTTCTCGGATGGGGGCTCGGAGCAGAAGACTTCAGTTGTGTTAGGGGGTCATCAATTGGAGGACACCCTGTTACATTTTGATTTGGGTGCTTCTATGCTAGGATTTAGTTCCTCCACGTTTATGGATCAGAATACTTGCTCTGATCTGGGACTGGGTTTTGGGTTTAAAGATTCAATGTAA

Protein Analysis

417

Amino Acids

44.75

Weight (kDa)

6.41

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 45 - 393 4.8e-08 Eukaryotic aspartyl protease
TAXi_N PF14543 46 - 204 1.1e-30 Xylanase inhibitor N-terminal
TAXi_C PF14541 239 - 393 6.9e-47 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19120
fragaria_vesca FvH4_4g14600
malus_domestica MD16G1185400.v1.1
prunus_persica Prupe.1G152000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0418211
rosa_laevigata RLG00000007885
rosa_roxburghii Rroxscaffold_5G00361420
rosa_rugosa Rorug04G0151200
rosa_samantha Rh4AG212900 Rh4BG217800 Rh4CG224600 Rh4DG211400
rosa_wichuraiana Rw4G018080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 910
AciI CCGC 4 cut(s) 198, 435, 578, 632
AclWI GGATC 2 cut(s) 142, 1203
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 1 cut(s) 1017
AcuI CTGAAG 2 cut(s) 752, 1086
AcyI GRCGYC 1 cut(s) 545
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 234, 369
AgsI TTSAA 4 cut(s) 55, 966, 1043, 1245
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 1 cut(s) 687
AjuI GAANNNNNNNTTGG 2 cut(s) 566, 598
AleI CACNNNNGTG 1 cut(s) 627
AluBI AGCT 5 cut(s) 270, 799, 834, 860, 872
AluI AGCT 5 cut(s) 270, 799, 834, 860, 872
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 3 cut(s) 323, 658, 768
Alw44I GTGCAC 1 cut(s) 465
AlwI GGATC 2 cut(s) 142, 1203
AoxI GGCC 4 cut(s) 199, 360, 426, 487
ApaLI GTGCAC 1 cut(s) 465
ApeKI GCWGC 6 cut(s) 5, 219, 557, 560, 872, 991
ApoI RAATTY 1 cut(s) 1017
AspLEI GCGC 1 cut(s) 973
AspS9I GGNCC 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 5 cut(s) 103, 343, 424, 699, 1018
BaeGI GKGCMC 1 cut(s) 469
BalI TGGCCA 1 cut(s) 362
BanI GGYRCC 1 cut(s) 910
BanII GRGCYC 1 cut(s) 1088
BbsI GAAGAC 3 cut(s) 231, 591, 1103
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 5 cut(s) 206, 544, 572, 859, 978
BccI CCATC 5 cut(s) 107, 437, 941, 997, 1073
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 689
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 3 cut(s) 323, 658, 768
BfaI CTAG 4 cut(s) 249, 491, 894, 1169
BfmI CTRYAG 2 cut(s) 558, 992
BfoI RGCGCY 1 cut(s) 974
BisI GCNGC 7 cut(s) 6, 199, 220, 558, 561, 873, 992
BlpI GCTNAGC 1 cut(s) 800
BlsI GCNGC 7 cut(s) 7, 200, 221, 559, 562, 874, 993
Bme1390I CCNGG 2 cut(s) 235, 689
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 3 cut(s) 571, 912, 934
BmrFI CCNGG 2 cut(s) 235, 689
BmrI ACTGGG 2 cut(s) 507, 1232
BmuI ACTGGG 2 cut(s) 507, 1232
BpiI GAAGAC 3 cut(s) 231, 591, 1103
BpmI CTGGAG 3 cut(s) 116, 152, 525
Bpu1102I GCTNAGC 1 cut(s) 800
BpuMI CCSGG 1 cut(s) 235
Bsa29I ATCGAT 2 cut(s) 189, 945
BsaAI YACGTR 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 545
BsaJI CCNNGG 2 cut(s) 154, 687
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 369
Bse1I ACTGG 3 cut(s) 133, 502, 1227
BseBI CCWGG 1 cut(s) 689
BseCI ATCGAT 2 cut(s) 189, 945
BseDI CCNNGG 2 cut(s) 154, 687
BseGI GGATG 2 cut(s) 585, 1084
BseLI CCNNNNNNNGG 2 cut(s) 234, 369
BseMII CTCAG 1 cut(s) 849
BseNI ACTGG 3 cut(s) 133, 502, 1227
BseSI GKGCMC 1 cut(s) 469
BseXI GCAGC 5 cut(s) 206, 544, 572, 859, 978
BshFI GGCC 4 cut(s) 201, 362, 428, 489
BshNI GGYRCC 1 cut(s) 910
BshVI ATCGAT 2 cut(s) 189, 945
BsiHKAI GWGCWC 1 cut(s) 469
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 1 cut(s) 1233
BslI CCNNNNNNNGG 2 cut(s) 234, 369
BsmAI GTCTC 3 cut(s) 323, 658, 768
BsmFI GGGAC 1 cut(s) 1233
BsmI GAATGC 1 cut(s) 951
BsnI GGCC 4 cut(s) 201, 362, 428, 489
Bsp1286I GDGCHC 2 cut(s) 469, 1088
Bsp143I GATC 4 cut(s) 147, 190, 1195, 1213
Bsp1720I GCTNAGC 1 cut(s) 800
BspACI CCGC 4 cut(s) 198, 435, 578, 632
BspANI GGCC 4 cut(s) 201, 362, 428, 489
BspCNI CTCAG 1 cut(s) 848
BspDI ATCGAT 2 cut(s) 189, 945
BspLI GGNNCC 3 cut(s) 571, 912, 934
BspMAI CTGCAG 2 cut(s) 562, 996
BspPI GGATC 2 cut(s) 142, 1203
BspT107I GGYRCC 1 cut(s) 910
BsrI ACTGG 3 cut(s) 133, 502, 1227
BssECI CCNNGG 2 cut(s) 154, 687
BssMI GATC 4 cut(s) 147, 190, 1195, 1213
BssNI GRCGYC 1 cut(s) 545
Bst2UI CCWGG 1 cut(s) 689
Bst4CI ACNGT 3 cut(s) 629, 808, 924
BstACI GRCGYC 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 1 cut(s) 870
BstDEI CTNAG 2 cut(s) 800, 835
BstDSI CCRYGG 1 cut(s) 154
BstF5I GGATG 2 cut(s) 585, 1084
BstH2I RGCGCY 1 cut(s) 974
BstHHI GCGC 1 cut(s) 973
BstKTI GATC 4 cut(s) 150, 193, 1198, 1216
BstMAI GTCTC 3 cut(s) 323, 658, 768
BstMBI GATC 4 cut(s) 147, 190, 1195, 1213
BstMWI GCNNNNNNNGC 4 cut(s) 318, 434, 500, 878
BstNI CCWGG 1 cut(s) 689
BstSCI CCNGG 2 cut(s) 233, 687
BstSFI CTRYAG 2 cut(s) 558, 992
BstSLI GKGCMC 1 cut(s) 469
BstSNI TACGTA 1 cut(s) 139
BstV1I GCAGC 5 cut(s) 206, 544, 572, 859, 978
BstV2I GAAGAC 3 cut(s) 231, 591, 1103
BstX2I RGATCY 1 cut(s) 147
BstXI CCANNNNNNTGG 2 cut(s) 210, 1192
BstYI RGATCY 1 cut(s) 147
Bsu15I ATCGAT 2 cut(s) 189, 945
BsuRI GGCC 4 cut(s) 201, 362, 428, 489
BsuTUI ATCGAT 2 cut(s) 189, 945
BtgI CCRYGG 1 cut(s) 154
BtgZI GCGATG 1 cut(s) 1065
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 2 cut(s) 585, 1084
BtsIMutI CAGTG 1 cut(s) 634
Cac8I GCNNGC 1 cut(s) 870
CfoI GCGC 1 cut(s) 973
Cfr13I GGNCC 1 cut(s) 200
ClaI ATCGAT 2 cut(s) 189, 945
CseI GACGC 1 cut(s) 534
Csp6I GTAC 1 cut(s) 136
CviAII CATG 4 cut(s) 462, 713, 889, 1022
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 2 cut(s) 800, 835
DpnI GATC 4 cut(s) 149, 192, 1197, 1215
DpnII GATC 4 cut(s) 147, 190, 1195, 1213
DraI TTTAAA 1 cut(s) 1237
EaeI YGGCCR 1 cut(s) 360
Eco105I TACGTA 1 cut(s) 139
Eco147I AGGCCT 1 cut(s) 489
Eco24I GRGCYC 1 cut(s) 1088
Eco57I CTGAAG 2 cut(s) 752, 1086
EcoRI GAATTC 1 cut(s) 1017
EcoRII CCWGG 1 cut(s) 687
EcoT38I GRGCYC 1 cut(s) 1088
FaeI CATG 4 cut(s) 465, 716, 892, 1025
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FaqI GGGAC 1 cut(s) 1233
FatI CATG 4 cut(s) 461, 712, 888, 1021
Fnu4HI GCNGC 7 cut(s) 6, 199, 220, 558, 561, 873, 992
FokI GGATG 2 cut(s) 572, 1091
FriOI GRGCYC 1 cut(s) 1088
Fsp4HI GCNGC 7 cut(s) 6, 199, 220, 558, 561, 873, 992
FspBI CTAG 4 cut(s) 249, 491, 894, 1169
GlaI GCGC 1 cut(s) 972
GluI GCNGC 7 cut(s) 6, 199, 220, 558, 561, 873, 992
GsuI CTGGAG 3 cut(s) 116, 152, 525
HaeII RGCGCY 1 cut(s) 974
HaeIII GGCC 4 cut(s) 201, 362, 428, 489
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 534
HhaI GCGC 1 cut(s) 973
Hin1I GRCGYC 1 cut(s) 545
Hin1II CATG 4 cut(s) 465, 716, 892, 1025
Hin6I GCGC 1 cut(s) 971
HinP1I GCGC 1 cut(s) 971
HincII GTYRAC 3 cut(s) 409, 703, 748
HindII GTYRAC 3 cut(s) 409, 703, 748
HindIII AAGCTT 1 cut(s) 858
HpaII CCGG 1 cut(s) 234
HphI GGTGA 5 cut(s) 103, 343, 424, 699, 1018
Hpy166II GTNNAC 4 cut(s) 409, 467, 703, 748
Hpy188I TCNGA 9 cut(s) 229, 483, 524, 743, 768, 1078, 1090, 1200, 1213
Hpy188III TCNNGA 3 cut(s) 249, 657, 966
Hpy8I GTNNAC 4 cut(s) 409, 467, 703, 748
HpyAV CCTTC 3 cut(s) 32, 407, 519
HpyCH4III ACNGT 3 cut(s) 629, 808, 924
HpyCH4IV ACGT 3 cut(s) 108, 138, 1187
HpyCH4V TGCA 5 cut(s) 16, 222, 467, 560, 994
HpyF10VI GCNNNNNNNGC 4 cut(s) 318, 434, 500, 878
HpyF3I CTNAG 2 cut(s) 800, 835
HpySE526I ACGT 3 cut(s) 108, 138, 1187
Hsp92I GRCGYC 1 cut(s) 545
Hsp92II CATG 4 cut(s) 465, 716, 892, 1025
HspAI GCGC 1 cut(s) 971
Kzo9I GATC 4 cut(s) 147, 190, 1195, 1213
LmnI GCTCC 2 cut(s) 575, 1090
Lsp1109I GCAGC 5 cut(s) 206, 544, 572, 859, 978
MaeI CTAG 4 cut(s) 249, 491, 894, 1169
MaeII ACGT 3 cut(s) 108, 138, 1187
MaeIII GTNAC 5 cut(s) 109, 139, 546, 691, 1140
MalI GATC 4 cut(s) 149, 192, 1197, 1215
MboI GATC 4 cut(s) 147, 190, 1195, 1213
MboII GAAGA 6 cut(s) 17, 20, 231, 237, 591, 1108
MfeI CAATTG 1 cut(s) 1124
MflI RGATCY 1 cut(s) 147
MhlI GDGCHC 2 cut(s) 469, 1088
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 8 cut(s) 49, 223, 442, 473, 552, 769, 1017, 1124
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 5 cut(s) 422, 513, 538, 698, 753
Mox20I TGGCCA 1 cut(s) 362
MscI TGGCCA 1 cut(s) 362
MseI TTAA 3 cut(s) 441, 795, 1236
MslI CAYNNNNRTG 3 cut(s) 356, 627, 1190
Msp20I TGGCCA 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 2 cut(s) 235, 689
MunI CAATTG 1 cut(s) 1124
Mva1269I GAATGC 1 cut(s) 951
MvaI CCWGG 1 cut(s) 689
MwoI GCNNNNNNNGC 4 cut(s) 318, 434, 500, 878
NciI CCSGG 1 cut(s) 235
NdeII GATC 4 cut(s) 147, 190, 1195, 1213
NlaIII CATG 4 cut(s) 465, 716, 892, 1025
NlaIV GGNNCC 3 cut(s) 571, 912, 934
NmeAIII GCCGAG 2 cut(s) 174, 195
NmuCI GTSAC 2 cut(s) 109, 546
OliI CACNNNNGTG 1 cut(s) 627
PceI AGGCCT 1 cut(s) 489
PcsI WCGNNNNNNNCGW 1 cut(s) 621
PctI GAATGC 1 cut(s) 951
PfeI GAWTC 5 cut(s) 230, 348, 902, 1039, 1241
PkrI GCNGC 7 cut(s) 7, 200, 221, 559, 562, 874, 993
PleI GAGTC 5 cut(s) 421, 513, 537, 698, 752
PpsI GAGTC 5 cut(s) 421, 513, 537, 698, 752
Ppu21I YACGTR 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 687
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 3 cut(s) 571, 912, 934
PspPI GGNCC 1 cut(s) 200
PstI CTGCAG 2 cut(s) 562, 996
PsuI RGATCY 1 cut(s) 147
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 3 cut(s) 356, 627, 1190
SaqAI TTAA 3 cut(s) 441, 795, 1236
SatI GCNGC 7 cut(s) 6, 199, 220, 558, 561, 873, 992
Sau3AI GATC 4 cut(s) 147, 190, 1195, 1213
Sau96I GGNCC 1 cut(s) 200
SchI GAGTC 5 cut(s) 422, 513, 538, 698, 753
ScrFI CCNGG 2 cut(s) 235, 689
SduI GDGCHC 2 cut(s) 469, 1088
SfcI CTRYAG 2 cut(s) 558, 992
SmiMI CAYNNNNRTG 3 cut(s) 356, 627, 1190
SnaBI TACGTA 1 cut(s) 139
Sse9I AATT 8 cut(s) 49, 223, 442, 473, 552, 769, 1017, 1124
SseBI AGGCCT 1 cut(s) 489
SsiI CCGC 4 cut(s) 198, 435, 578, 632
SspMI CTAG 4 cut(s) 249, 491, 894, 1169
StuI AGGCCT 1 cut(s) 489
StyD4I CCNGG 2 cut(s) 233, 687
TaaI ACNGT 3 cut(s) 629, 808, 924
TaiI ACGT 3 cut(s) 111, 141, 1190
TaqI TCGA 3 cut(s) 189, 532, 945
TasI AATT 8 cut(s) 49, 223, 442, 473, 552, 769, 1017, 1124
TauI GCSGC 1 cut(s) 201
TfiI GAWTC 5 cut(s) 230, 348, 902, 1039, 1241
Tru1I TTAA 3 cut(s) 441, 795, 1236
Tru9I TTAA 3 cut(s) 441, 795, 1236
TscAI CASTG 1 cut(s) 634
TseFI GTSAC 2 cut(s) 109, 546
TseI GCWGC 6 cut(s) 5, 219, 557, 560, 872, 991
Tsp45I GTSAC 2 cut(s) 109, 546
TspDTI ATGAA 8 cut(s) 231, 450, 574, 591, 712, 899, 1010, 1068
TspGWI ACGGA 2 cut(s) 390, 1024
TspRI CASTG 1 cut(s) 634
VneI GTGCAC 1 cut(s) 465
XapI RAATTY 1 cut(s) 1017
XbaI TCTAGA 1 cut(s) 248
XspI CTAG 4 cut(s) 249, 491, 894, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.