Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0017142

Physical Location & Seq

Genomic Coordinates Reverse (-)

40655340 .. 40656903

Gene Structure 1564 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG211400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1239 bp

ATGGCTGCTTTTCTGCACTTCCTTCTTCTTCTCTGCTCTTTTGCCTACAACTTCAACACATCTCAATCCAAACATACTCACCCACAACAAAAAGGTGCCATCTTTCACGTCACCAAAGATCTATCAACCCTCCAGTACGTGACCCAGATCCACCACGGCACTCCTCTTCAACCCACCAACCTCGTAATCGATCTCGGCGGCCCTTTCCTCTGGCTCGGCTGCGCTTCCGATTCCGGGTCTTCATCTTCTAGAACTCTCATCTCTCACAGCTCAATCAAGTGCTTATCAGCCAACCCCAACACCCAAAAAAGCAAGTCCAGCAAGTGTGAGACTTTCCCACAAAACAGAATCACCCAAATGGCCACACAAGGAGATTTGGCAGAAGACATCATAGCCCTTCCGTTCGTTGATGTGTCCAAAACCACCGTTGATTCCCTTTTCTTATTTTCTTGTGCACCCAAATTGCTTCTGAAAGGCCTAGCATCTGGGGCTAGAGGAATGTTAGGACTCGGAAGGAGTCGAGTTTCGGTGGCGTCGCAAATTGCTGCAGCATTTGGTTCCAAACCGCAGTTCATCCTCTGCCTGTCTTCATCAAACGGGGTCGTTTTACACGACAGTGGGGAATATGGTTCTTATTTTGTCTCTGGAGTTTCAGGCTCACTCACACACACACCCCTGGTTACCAAAGTCGACTCACCACATGAGTATTTCATCAACCAGAAGTCAATCAGAGTCAACGGGAAGCAATTGTCTCTGAATTACAAAGAGGGTCTTGGAGGGATTAAGCTAAGCACAGTTGTTCCCTACTCTACTATGGAGAGCTCAGTCTATGCTGTATTTGTCAAAGCTTATGAGCAAGCTGCTGTGGCTATGAACATGACTAGGGTGGCTTCTGTGGCACCATTTGGACTGTGTTTTGGTTCCAAAAACATCGATGGCATTCGGGTTGGTTCAAGAGCGCCTGTTGTTGATTTGGGGCTGCAGAGTGAGATGGTGAAGTGGAGAATTCATGGTAGAAACTCCATGGTTCAAGTGAGCGATGAAGTTATGTGTTTGGGGTTCTTGGATGGTGGTTTGGAGCAGAAGACTTCAATTGTGTTAGGGGGTCATCAATTGGAGGATACCCTGTTATATTTTGATTTGGGTGCTTCTATGCTAGGATTTAGTTCTTCCACGTTGATGGATCAGAATACTTGCTCTGATCTGGGACTGGGTTTTGGGTTTAAAGATTCAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0005488 GO:0005515 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0042802 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

412

Amino Acids

44.24

Weight (kDa)

8.3

Isoelectric Point (pI)

32.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	45 - 389	3.4e-07	Eukaryotic aspartyl protease
TAXi_N	PF14543	46 - 200	3.2e-33	Xylanase inhibitor N-terminal
TAXi_C	PF14541	235 - 389	2.6e-47	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19120
fragaria_vesca	FvH4_4g14600
malus_domestica	MD16G1185400.v1.1
prunus_persica	Prupe.1G152000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0418211
rosa_laevigata	RLG00000007885
rosa_roxburghii	Rroxscaffold_5G00361420
rosa_rugosa	Rorug04G0151200
rosa_samantha	Rh4AG212900 Rh4BG217800 Rh4CG224600 Rh4DG211400
rosa_wichuraiana	Rw4G018080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	95, 898
AccI	GTMKAC	1 cut(s)	690
AciI	CCGC	2 cut(s)	198, 566
AclWI	GGATC	2 cut(s)	142, 1191
AcoI	YGGCCR	1 cut(s)	360
AcsI	RAATTY	1 cut(s)	1005
AcyI	GRCGYC	1 cut(s)	533
AfaI	GTAC	1 cut(s)	137
AfiI	CCNNNNNNNGG	1 cut(s)	234
AgsI	TTSAA	6 cut(s)	55, 170, 954, 1031, 1092, 1233
AjiI	CACGTC	1 cut(s)	109
AjnI	CCWGG	1 cut(s)	675
AjuI	GAANNNNNNNTTGG	2 cut(s)	554, 586
AleI	CACNNNNGTG	1 cut(s)	615
AluBI	AGCT	5 cut(s)	270, 787, 822, 848, 860
AluI	AGCT	5 cut(s)	270, 787, 822, 848, 860
Alw21I	GWGCWC	2 cut(s)	457, 824
Alw26I	GTCTC	3 cut(s)	323, 646, 756
Alw44I	GTGCAC	1 cut(s)	453
AlwI	GGATC	2 cut(s)	142, 1191
AoxI	GGCC	3 cut(s)	199, 360, 475
ApaLI	GTGCAC	1 cut(s)	453
ApeKI	GCWGC	6 cut(s)	5, 219, 545, 548, 860, 979
ApoI	RAATTY	1 cut(s)	1005
AspLEI	GCGC	2 cut(s)	224, 961
AspS9I	GGNCC	1 cut(s)	200
AsuC2I	CCSGG	1 cut(s)	235
AsuHPI	GGTGA	5 cut(s)	71, 103, 343, 687, 1006
BaeGI	GKGCMC	1 cut(s)	457
BalI	TGGCCA	1 cut(s)	362
BanI	GGYRCC	2 cut(s)	95, 898
BanII	GRGCYC	1 cut(s)	824
BbsI	GAAGAC	4 cut(s)	231, 390, 579, 1091
Bbv12I	GWGCWC	2 cut(s)	457, 824
BbvI	GCAGC	5 cut(s)	206, 532, 560, 847, 966
BccI	CCATC	5 cut(s)	107, 929, 985, 1061, 1174
BceAI	ACGGC	1 cut(s)	172
BciT130I	CCWGG	1 cut(s)	677
BciVI	GTATCC	1 cut(s)	1114
BcnI	CCSGG	1 cut(s)	235
BcoDI	GTCTC	3 cut(s)	323, 646, 756
BfaI	CTAG	5 cut(s)	249, 479, 492, 882, 1157
BfmI	CTRYAG	2 cut(s)	546, 980
BfoI	RGCGCY	1 cut(s)	962
BfuI	GTATCC	1 cut(s)	1114
BglII	AGATCT	1 cut(s)	118
BisI	GCNGC	7 cut(s)	6, 199, 220, 546, 549, 861, 980
BlpI	GCTNAGC	1 cut(s)	788
BlsI	GCNGC	7 cut(s)	7, 200, 221, 547, 550, 862, 981
Bme1390I	CCNGG	2 cut(s)	235, 677
BmgBI	CACGTC	1 cut(s)	109
BmgT120I	GGNCC	1 cut(s)	200
BmiI	GGNNCC	4 cut(s)	97, 559, 900, 922
BmrFI	CCNGG	2 cut(s)	235, 677
BmrI	ACTGGG	1 cut(s)	1220
BmsI	GCATC	1 cut(s)	491
BmuI	ACTGGG	1 cut(s)	1220
BpiI	GAAGAC	4 cut(s)	231, 390, 579, 1091
BpmI	CTGGAG	2 cut(s)	116, 666
Bpu1102I	GCTNAGC	1 cut(s)	788
BpuMI	CCSGG	1 cut(s)	235
Bsa29I	ATCGAT	2 cut(s)	189, 933
BsaAI	YACGTR	1 cut(s)	139
BsaHI	GRCGYC	1 cut(s)	533
BsaJI	CCNNGG	3 cut(s)	154, 675, 1023
Bsc4I	CCNNNNNNNGG	1 cut(s)	234
Bse1I	ACTGG	2 cut(s)	133, 1215
BseBI	CCWGG	1 cut(s)	677
BseCI	ATCGAT	2 cut(s)	189, 933
BseDI	CCNNGG	3 cut(s)	154, 675, 1023
BseGI	GGATG	2 cut(s)	573, 1072
BseLI	CCNNNNNNNGG	1 cut(s)	234
BseMII	CTCAG	1 cut(s)	837
BseNI	ACTGG	2 cut(s)	133, 1215
BseRI	GAGGAG	1 cut(s)	153
BseSI	GKGCMC	1 cut(s)	457
BseXI	GCAGC	5 cut(s)	206, 532, 560, 847, 966
BshFI	GGCC	3 cut(s)	201, 362, 477
BshNI	GGYRCC	2 cut(s)	95, 898
BshVI	ATCGAT	2 cut(s)	189, 933
BsiHKAI	GWGCWC	2 cut(s)	457, 824
BsiSI	CCGG	1 cut(s)	234
BslFI	GGGAC	1 cut(s)	1221
BslI	CCNNNNNNNGG	1 cut(s)	234
BsmAI	GTCTC	3 cut(s)	323, 646, 756
BsmFI	GGGAC	1 cut(s)	1221
BsmI	GAATGC	1 cut(s)	939
BsnI	GGCC	3 cut(s)	201, 362, 477
Bsp1286I	GDGCHC	2 cut(s)	457, 824
Bsp143I	GATC	5 cut(s)	118, 147, 190, 1183, 1201
Bsp1720I	GCTNAGC	1 cut(s)	788
Bsp19I	CCATGG	1 cut(s)	1023
BspACI	CCGC	2 cut(s)	198, 566
BspANI	GGCC	3 cut(s)	201, 362, 477
BspCNI	CTCAG	1 cut(s)	836
BspDI	ATCGAT	2 cut(s)	189, 933
BspLI	GGNNCC	4 cut(s)	97, 559, 900, 922
BspMAI	CTGCAG	2 cut(s)	550, 984
BspPI	GGATC	2 cut(s)	142, 1191
BspT107I	GGYRCC	2 cut(s)	95, 898
BsrI	ACTGG	2 cut(s)	133, 1215
BssECI	CCNNGG	3 cut(s)	154, 675, 1023
BssMI	GATC	5 cut(s)	118, 147, 190, 1183, 1201
BssNI	GRCGYC	1 cut(s)	533
BssT1I	CCWWGG	1 cut(s)	1023
Bst2UI	CCWGG	1 cut(s)	677
Bst4CI	ACNGT	4 cut(s)	427, 617, 796, 912
Bst6I	CTCTTC	1 cut(s)	171
BstACI	GRCGYC	1 cut(s)	533
BstBAI	YACGTR	1 cut(s)	139
BstC8I	GCNNGC	1 cut(s)	858
BstDEI	CTNAG	2 cut(s)	788, 823
BstDSI	CCRYGG	2 cut(s)	154, 1023
BstEII	GGTNACC	1 cut(s)	679
BstF5I	GGATG	2 cut(s)	573, 1072
BstH2I	RGCGCY	1 cut(s)	962
BstHHI	GCGC	2 cut(s)	224, 961
BstKTI	GATC	5 cut(s)	121, 150, 193, 1186, 1204
BstMAI	GTCTC	3 cut(s)	323, 646, 756
BstMBI	GATC	5 cut(s)	118, 147, 190, 1183, 1201
BstMWI	GCNNNNNNNGC	4 cut(s)	318, 488, 866, 896
BstNI	CCWGG	1 cut(s)	677
BstPI	GGTNACC	1 cut(s)	679
BstSCI	CCNGG	2 cut(s)	233, 675
BstSFI	CTRYAG	2 cut(s)	546, 980
BstSLI	GKGCMC	1 cut(s)	457
BstV1I	GCAGC	5 cut(s)	206, 532, 560, 847, 966
BstV2I	GAAGAC	4 cut(s)	231, 390, 579, 1091
BstX2I	RGATCY	2 cut(s)	118, 147
BstXI	CCANNNNNNTGG	1 cut(s)	1180
BstYI	RGATCY	2 cut(s)	118, 147
Bsu15I	ATCGAT	2 cut(s)	189, 933
BsuI	GTATCC	1 cut(s)	1114
BsuRI	GGCC	3 cut(s)	201, 362, 477
BsuTUI	ATCGAT	2 cut(s)	189, 933
BtgI	CCRYGG	2 cut(s)	154, 1023
BtgZI	GCGATG	1 cut(s)	1053
BtrI	CACGTC	1 cut(s)	109
BtsCI	GGATG	2 cut(s)	573, 1072
BtsIMutI	CAGTG	1 cut(s)	622
Cac8I	GCNNGC	1 cut(s)	858
CfoI	GCGC	2 cut(s)	224, 961
Cfr13I	GGNCC	1 cut(s)	200
ClaI	ATCGAT	2 cut(s)	189, 933
CseI	GACGC	1 cut(s)	522
Csp6I	GTAC	1 cut(s)	136
CviAII	CATG	4 cut(s)	701, 877, 1010, 1024
CviQI	GTAC	1 cut(s)	136
DdeI	CTNAG	2 cut(s)	788, 823
DpnI	GATC	5 cut(s)	120, 149, 192, 1185, 1203
DpnII	GATC	5 cut(s)	118, 147, 190, 1183, 1201
DraI	TTTAAA	1 cut(s)	1225
EaeI	YGGCCR	1 cut(s)	360
Eam1104I	CTCTTC	1 cut(s)	171
EarI	CTCTTC	1 cut(s)	171
Ecl136II	GAGCTC	1 cut(s)	822
Eco130I	CCWWGG	1 cut(s)	1023
Eco147I	AGGCCT	1 cut(s)	477
Eco24I	GRGCYC	1 cut(s)	824
Eco53kI	GAGCTC	1 cut(s)	822
Eco91I	GGTNACC	1 cut(s)	679
EcoICRI	GAGCTC	1 cut(s)	822
EcoO65I	GGTNACC	1 cut(s)	679
EcoRI	GAATTC	1 cut(s)	1005
EcoRII	CCWGG	1 cut(s)	675
EcoT14I	CCWWGG	1 cut(s)	1023
EcoT38I	GRGCYC	1 cut(s)	824
ErhI	CCWWGG	1 cut(s)	1023
FaeI	CATG	4 cut(s)	704, 880, 1013, 1027
FalI	AAGNNNNNCTT	2 cut(s)	756, 788
FaqI	GGGAC	1 cut(s)	1221
FatI	CATG	4 cut(s)	700, 876, 1009, 1023
FblI	GTMKAC	1 cut(s)	690
Fnu4HI	GCNGC	7 cut(s)	6, 199, 220, 546, 549, 861, 980
FokI	GGATG	2 cut(s)	560, 1079
FriOI	GRGCYC	1 cut(s)	824
Fsp4HI	GCNGC	7 cut(s)	6, 199, 220, 546, 549, 861, 980
FspBI	CTAG	5 cut(s)	249, 479, 492, 882, 1157
GlaI	GCGC	2 cut(s)	223, 960
GluI	GCNGC	7 cut(s)	6, 199, 220, 546, 549, 861, 980
GsuI	CTGGAG	2 cut(s)	116, 666
HaeII	RGCGCY	1 cut(s)	962
HaeIII	GGCC	3 cut(s)	201, 362, 477
HapII	CCGG	1 cut(s)	234
HgaI	GACGC	1 cut(s)	522
HhaI	GCGC	2 cut(s)	224, 961
Hin1I	GRCGYC	1 cut(s)	533
Hin1II	CATG	4 cut(s)	704, 880, 1013, 1027
Hin6I	GCGC	2 cut(s)	222, 959
HinP1I	GCGC	2 cut(s)	222, 959
HincII	GTYRAC	2 cut(s)	691, 736
HindII	GTYRAC	2 cut(s)	691, 736
HindIII	AAGCTT	1 cut(s)	846
HinfI	GANTC	8 cut(s)	230, 348, 431, 507, 517, 692, 732, 1229
HpaII	CCGG	1 cut(s)	234
HphI	GGTGA	5 cut(s)	71, 103, 343, 687, 1006
Hpy166II	GTNNAC	3 cut(s)	455, 691, 736
Hpy188I	TCNGA	7 cut(s)	229, 471, 512, 731, 756, 1188, 1201
Hpy188III	TCNNGA	3 cut(s)	249, 645, 954
Hpy8I	GTNNAC	3 cut(s)	455, 691, 736
Hpy99I	CGWCG	1 cut(s)	538
HpyAV	CCTTC	3 cut(s)	32, 407, 507
HpyCH4III	ACNGT	4 cut(s)	427, 617, 796, 912
HpyCH4IV	ACGT	3 cut(s)	108, 138, 1175
HpyCH4V	TGCA	4 cut(s)	16, 455, 548, 982
HpyF10VI	GCNNNNNNNGC	4 cut(s)	318, 488, 866, 896
HpyF3I	CTNAG	2 cut(s)	788, 823
HpySE526I	ACGT	3 cut(s)	108, 138, 1175
Hsp92I	GRCGYC	1 cut(s)	533
Hsp92II	CATG	4 cut(s)	704, 880, 1013, 1027
HspAI	GCGC	2 cut(s)	222, 959
Kzo9I	GATC	5 cut(s)	118, 147, 190, 1183, 1201
LmnI	GCTCC	1 cut(s)	1078
Lsp1109I	GCAGC	5 cut(s)	206, 532, 560, 847, 966
LweI	GCATC	1 cut(s)	491
MaeI	CTAG	5 cut(s)	249, 479, 492, 882, 1157
MaeII	ACGT	3 cut(s)	108, 138, 1175
MaeIII	GTNAC	3 cut(s)	109, 139, 679
MalI	GATC	5 cut(s)	120, 149, 192, 1185, 1203
MboI	GATC	5 cut(s)	118, 147, 190, 1183, 1201
MboII	GAAGA	9 cut(s)	17, 20, 158, 231, 237, 395, 579, 1096, 1161
MfeI	CAATTG	3 cut(s)	746, 1092, 1112
MflI	RGATCY	2 cut(s)	118, 147
MhlI	GDGCHC	2 cut(s)	457, 824
MlsI	TGGCCA	1 cut(s)	362
MluCI	AATT	7 cut(s)	461, 540, 746, 757, 1005, 1092, 1112
MluNI	TGGCCA	1 cut(s)	362
MlyI	GAGTC	4 cut(s)	501, 526, 686, 741
MnlI	CCTC	9 cut(s)	140, 174, 191, 218, 488, 587, 760, 770, 1111
Mox20I	TGGCCA	1 cut(s)	362
MscI	TGGCCA	1 cut(s)	362
MseI	TTAA	2 cut(s)	783, 1224
MslI	CAYNNNNRTG	3 cut(s)	356, 615, 1178
Msp20I	TGGCCA	1 cut(s)	362
MspI	CCGG	1 cut(s)	234
MspR9I	CCNGG	2 cut(s)	235, 677
MunI	CAATTG	3 cut(s)	746, 1092, 1112
Mva1269I	GAATGC	1 cut(s)	939
MvaI	CCWGG	1 cut(s)	677
MwoI	GCNNNNNNNGC	4 cut(s)	318, 488, 866, 896
NciI	CCSGG	1 cut(s)	235
NcoI	CCATGG	1 cut(s)	1023
NdeII	GATC	5 cut(s)	118, 147, 190, 1183, 1201
NlaIII	CATG	4 cut(s)	704, 880, 1013, 1027
NlaIV	GGNNCC	4 cut(s)	97, 559, 900, 922
NmeAIII	GCCGAG	2 cut(s)	174, 195
NmuCI	GTSAC	2 cut(s)	109, 139
OliI	CACNNNNGTG	1 cut(s)	615
PceI	AGGCCT	1 cut(s)	477
PcsI	WCGNNNNNNNCGW	1 cut(s)	609
PctI	GAATGC	1 cut(s)	939
PfeI	GAWTC	4 cut(s)	230, 348, 431, 1229
PkrI	GCNGC	7 cut(s)	7, 200, 221, 547, 550, 862, 981
PleI	GAGTC	4 cut(s)	501, 525, 686, 740
PpsI	GAGTC	4 cut(s)	501, 525, 686, 740
Ppu21I	YACGTR	1 cut(s)	139
Psp124BI	GAGCTC	1 cut(s)	824
Psp6I	CCWGG	1 cut(s)	675
PspEI	GGTNACC	1 cut(s)	679
PspGI	CCWGG	1 cut(s)	675
PspN4I	GGNNCC	4 cut(s)	97, 559, 900, 922
PspPI	GGNCC	1 cut(s)	200
PstI	CTGCAG	2 cut(s)	550, 984
PsuI	RGATCY	2 cut(s)	118, 147
RsaI	GTAC	1 cut(s)	137
RsaNI	GTAC	1 cut(s)	136
RseI	CAYNNNNRTG	3 cut(s)	356, 615, 1178
SacI	GAGCTC	1 cut(s)	824
SalI	GTCGAC	1 cut(s)	689
SaqAI	TTAA	2 cut(s)	783, 1224
SatI	GCNGC	7 cut(s)	6, 199, 220, 546, 549, 861, 980
Sau3AI	GATC	5 cut(s)	118, 147, 190, 1183, 1201
Sau96I	GGNCC	1 cut(s)	200
SchI	GAGTC	4 cut(s)	501, 526, 686, 741
ScrFI	CCNGG	2 cut(s)	235, 677
SduI	GDGCHC	2 cut(s)	457, 824
SfaNI	GCATC	1 cut(s)	491
SfcI	CTRYAG	2 cut(s)	546, 980
SmiMI	CAYNNNNRTG	3 cut(s)	356, 615, 1178
Sse9I	AATT	7 cut(s)	461, 540, 746, 757, 1005, 1092, 1112
SseBI	AGGCCT	1 cut(s)	477
SsiI	CCGC	2 cut(s)	198, 566
SspMI	CTAG	5 cut(s)	249, 479, 492, 882, 1157
SstI	GAGCTC	1 cut(s)	824
StuI	AGGCCT	1 cut(s)	477
StyD4I	CCNGG	2 cut(s)	233, 675
StyI	CCWWGG	1 cut(s)	1023
TaaI	ACNGT	4 cut(s)	427, 617, 796, 912
TaiI	ACGT	3 cut(s)	111, 141, 1178
TaqI	TCGA	4 cut(s)	189, 520, 690, 933
TasI	AATT	7 cut(s)	461, 540, 746, 757, 1005, 1092, 1112
TauI	GCSGC	1 cut(s)	201
TfiI	GAWTC	4 cut(s)	230, 348, 431, 1229
Tru1I	TTAA	2 cut(s)	783, 1224
Tru9I	TTAA	2 cut(s)	783, 1224
TscAI	CASTG	1 cut(s)	622
TseFI	GTSAC	2 cut(s)	109, 139
TseI	GCWGC	6 cut(s)	5, 219, 545, 548, 860, 979
Tsp45I	GTSAC	2 cut(s)	109, 139
TspDTI	ATGAA	7 cut(s)	231, 562, 579, 700, 887, 998, 1056
TspGWI	ACGGA	1 cut(s)	390
TspRI	CASTG	1 cut(s)	622
VneI	GTGCAC	1 cut(s)	453
XapI	RAATTY	1 cut(s)	1005
XbaI	TCTAGA	1 cut(s)	248
XmiI	GTMKAC	1 cut(s)	690
XspI	CTAG	5 cut(s)	249, 479, 492, 882, 1157

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.