Rh4AG212900

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017142
Physical Location & Seq
Reverse (-)
50683611 .. 50686110
2500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG212900.1

Sequence Viewer

Length: 609 bp
ATGTTAGGACTCGGAAGGAGTCGAGTTTCGGTGGCGTCACAAATTGCTGCAGCATTTGGTTCCAAACCGCAGTTCATCCTCTGCCTGTCTTCATCAAACGGGGTCGTTTTGCACGACAATGGGGAATATGGCTCACTCACATACACACCCCTGGTTACTAAAGTTGACTCACCACATGAGTATTTCATCAACCTGAAGTCAATAAGAGTCAACGGGAAGCAATTGTCTCTGAATTACAAAGAGGGTCTTGGAGGGATTAAGCTAAGCACAGTTGTTCCCTACTCTACGTTGGAAAGCTCAGTCTATGCTGTATTTGTCAAAGCTTATGAGCAAGCTGCTGAGGCTATGAACATGACTAGGGTGGCTTCTGTGGCACCATTTGGACTGTGTTTTGGTTCCAAAAACATCGATGGCATTCGGACCCCAATCTACAATGCTGTGGTTAGTGCATTTGTCAAAGAGCTCGTTCACATGGCAAGAGTGACACCTGTAGCACCTTCCGGAGCATGCTTCGACCCAAAGAAGATTGGTGTTACGCTGCTGGGTTGGGCTGTACCTGCCATTGATCTTGTTTTGAGACTTTACACTGGAGGCCTTCTTGGCATATGA

Protein Analysis

202

Amino Acids

21.52

Weight (kDa)

9.26

Isoelectric Point (pI)

29.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_C PF14541 60 - 136 1.1e-19 Xylanase inhibitor C-terminal
TAXi_C PF14541 140 - 193 1.4e-09 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0017142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19120
fragaria_vesca FvH4_4g14600
malus_domestica MD16G1185400.v1.1
prunus_persica Prupe.1G152000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0418211
rosa_laevigata RLG00000007885
rosa_roxburghii Rroxscaffold_5G00361420
rosa_rugosa Rorug04G0151200
rosa_samantha Rh4AG212900 Rh4BG217800 Rh4CG224600 Rh4DG211400
rosa_wichuraiana Rw4G018080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 373
AccIII TCCGGA 1 cut(s) 500
AciI CCGC 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 215
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 1 cut(s) 555
AjnI CCWGG 1 cut(s) 150
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 5 cut(s) 262, 297, 323, 335, 463
AluI AGCT 5 cut(s) 262, 297, 323, 335, 463
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 2 cut(s) 231, 571
Aor13HI TCCGGA 1 cut(s) 500
AoxI GGCC 1 cut(s) 592
ApeKI GCWGC 4 cut(s) 47, 50, 335, 538
AspS9I GGNCC 1 cut(s) 420
AsuHPI GGTGA 1 cut(s) 162
AvaII GGWCC 1 cut(s) 420
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 465
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 4 cut(s) 34, 62, 322, 525
BccI CCATC 1 cut(s) 404
BciT130I CCWGG 1 cut(s) 152
BcoDI GTCTC 2 cut(s) 231, 571
BfaI CTAG 1 cut(s) 357
BfmI CTRYAG 2 cut(s) 48, 489
BfuAI ACCTGC 1 cut(s) 565
BglI GCCNNNNNGGC 1 cut(s) 600
BisI GCNGC 4 cut(s) 48, 51, 336, 539
BlpI GCTNAGC 1 cut(s) 263
BlsI GCNGC 4 cut(s) 49, 52, 337, 540
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 1 cut(s) 420
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 4 cut(s) 61, 375, 397, 422
BmrFI CCNGG 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 609
Bpu10I CCTNAGC 1 cut(s) 339
Bpu1102I GCTNAGC 1 cut(s) 263
Bsa29I ATCGAT 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 150
BsaWI WCCGGW 1 cut(s) 500
Bse1I ACTGG 1 cut(s) 592
BseAI TCCGGA 1 cut(s) 500
BseBI CCWGG 1 cut(s) 152
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 75
BseMII CTCAG 2 cut(s) 312, 330
BseNI ACTGG 1 cut(s) 592
BseXI GCAGC 4 cut(s) 34, 62, 322, 525
BseYI CCCAGC 1 cut(s) 541
BshFI GGCC 1 cut(s) 594
BshNI GGYRCC 1 cut(s) 373
BshVI ATCGAT 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 465
BsiSI CCGG 1 cut(s) 501
BsmAI GTCTC 2 cut(s) 231, 571
BsmI GAATGC 1 cut(s) 414
BsnI GGCC 1 cut(s) 594
Bsp1286I GDGCHC 1 cut(s) 465
Bsp13I TCCGGA 1 cut(s) 500
Bsp143I GATC 1 cut(s) 565
Bsp1720I GCTNAGC 1 cut(s) 263
BspACI CCGC 1 cut(s) 68
BspANI GGCC 1 cut(s) 594
BspCNI CTCAG 2 cut(s) 311, 331
BspDI ATCGAT 1 cut(s) 408
BspEI TCCGGA 1 cut(s) 500
BspLI GGNNCC 4 cut(s) 61, 375, 397, 422
BspMAI CTGCAG 1 cut(s) 52
BspMI ACCTGC 1 cut(s) 565
BspT107I GGYRCC 1 cut(s) 373
BsrI ACTGG 1 cut(s) 592
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 1 cut(s) 565
BssNI GRCGYC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 2 cut(s) 271, 387
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 333, 508
BstDEI CTNAG 3 cut(s) 263, 298, 339
BstF5I GGATG 1 cut(s) 75
BstKTI GATC 1 cut(s) 568
BstMAI GTCTC 2 cut(s) 231, 571
BstMBI GATC 1 cut(s) 565
BstMWI GCNNNNNNNGC 4 cut(s) 341, 371, 557, 600
BstNI CCWGG 1 cut(s) 152
BstNSI RCATGY 1 cut(s) 510
BstSCI CCNGG 1 cut(s) 150
BstSFI CTRYAG 2 cut(s) 48, 489
BstV1I GCAGC 4 cut(s) 34, 62, 322, 525
BstV2I GAAGAC 1 cut(s) 81
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 1 cut(s) 594
BsuTUI ATCGAT 1 cut(s) 408
BtsCI GGATG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 585
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 2 cut(s) 333, 508
Cfr13I GGNCC 1 cut(s) 420
ClaI ATCGAT 1 cut(s) 408
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 554
CviAII CATG 4 cut(s) 176, 352, 472, 507
CviQI GTAC 1 cut(s) 554
DdeI CTNAG 3 cut(s) 263, 298, 339
DpnI GATC 1 cut(s) 567
DpnII GATC 1 cut(s) 565
Ecl136II GAGCTC 1 cut(s) 463
Eco147I AGGCCT 1 cut(s) 594
Eco24I GRGCYC 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 420
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 215
EcoICRI GAGCTC 1 cut(s) 463
EcoRII CCWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 465
FaeI CATG 4 cut(s) 179, 355, 475, 510
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FatI CATG 4 cut(s) 175, 351, 471, 506
FauNDI CATATG 1 cut(s) 605
Fnu4HI GCNGC 4 cut(s) 48, 51, 336, 539
FokI GGATG 1 cut(s) 62
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 4 cut(s) 48, 51, 336, 539
FspBI CTAG 1 cut(s) 357
GluI GCNGC 4 cut(s) 48, 51, 336, 539
GsaI CCCAGC 1 cut(s) 545
GsuI CTGGAG 1 cut(s) 609
HaeIII GGCC 1 cut(s) 594
HapII CCGG 1 cut(s) 501
HgaI GACGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 4 cut(s) 179, 355, 475, 510
HincII GTYRAC 2 cut(s) 166, 211
HindII GTYRAC 2 cut(s) 166, 211
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 4 cut(s) 9, 19, 167, 207
HpaII CCGG 1 cut(s) 501
HphI GGTGA 1 cut(s) 162
Hpy166II GTNNAC 3 cut(s) 166, 211, 469
Hpy188I TCNGA 3 cut(s) 14, 231, 420
Hpy188III TCNNGA 1 cut(s) 501
Hpy8I GTNNAC 3 cut(s) 166, 211, 469
HpyAV CCTTC 3 cut(s) 9, 507, 605
HpyCH4III ACNGT 2 cut(s) 271, 387
HpyCH4IV ACGT 1 cut(s) 287
HpyCH4V TGCA 3 cut(s) 50, 112, 449
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 371, 557, 600
HpyF3I CTNAG 3 cut(s) 263, 298, 339
HpySE526I ACGT 1 cut(s) 287
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 4 cut(s) 179, 355, 475, 510
Kpn2I TCCGGA 1 cut(s) 500
Kzo9I GATC 1 cut(s) 565
LmnI GCTCC 1 cut(s) 503
LpnPI CCDG 9 cut(s) 98, 137, 164, 206, 501, 514, 527, 570, 573
Lsp1109I GCAGC 4 cut(s) 34, 62, 322, 525
MaeI CTAG 1 cut(s) 357
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 4 cut(s) 36, 154, 481, 532
MalI GATC 1 cut(s) 567
MboI GATC 1 cut(s) 565
MboII GAAGA 2 cut(s) 81, 535
MfeI CAATTG 1 cut(s) 221
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 3 cut(s) 42, 221, 232
MlyI GAGTC 4 cut(s) 3, 28, 161, 216
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 5 cut(s) 89, 235, 245, 334, 584
MroI TCCGGA 1 cut(s) 500
MseI TTAA 1 cut(s) 258
MslI CAYNNNNRTG 1 cut(s) 117
MspI CCGG 1 cut(s) 501
MspR9I CCNGG 1 cut(s) 152
MunI CAATTG 1 cut(s) 221
Mva1269I GAATGC 1 cut(s) 414
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 4 cut(s) 341, 371, 557, 600
NdeI CATATG 1 cut(s) 605
NdeII GATC 1 cut(s) 565
NlaIII CATG 4 cut(s) 179, 355, 475, 510
NlaIV GGNNCC 4 cut(s) 61, 375, 397, 422
NmuCI GTSAC 2 cut(s) 36, 481
NspI RCATGY 1 cut(s) 510
PaeI GCATGC 1 cut(s) 510
PceI AGGCCT 1 cut(s) 594
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 414
PkrI GCNGC 4 cut(s) 49, 52, 337, 540
PleI GAGTC 4 cut(s) 3, 27, 161, 215
PpsI GAGTC 4 cut(s) 3, 27, 161, 215
Psp124BI GAGCTC 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 150
PspFI CCCAGC 1 cut(s) 541
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 4 cut(s) 61, 375, 397, 422
PspPI GGNCC 1 cut(s) 420
PstI CTGCAG 1 cut(s) 52
RsaI GTAC 1 cut(s) 555
RsaNI GTAC 1 cut(s) 554
RseI CAYNNNNRTG 1 cut(s) 117
SacI GAGCTC 1 cut(s) 465
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 4 cut(s) 48, 51, 336, 539
Sau3AI GATC 1 cut(s) 565
Sau96I GGNCC 1 cut(s) 420
SchI GAGTC 4 cut(s) 3, 28, 161, 216
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 1 cut(s) 465
SfcI CTRYAG 2 cut(s) 48, 489
SinI GGWCC 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 117
SphI GCATGC 1 cut(s) 510
Sse9I AATT 3 cut(s) 42, 221, 232
SseBI AGGCCT 1 cut(s) 594
SsiI CCGC 1 cut(s) 68
SspMI CTAG 1 cut(s) 357
SstI GAGCTC 1 cut(s) 465
StuI AGGCCT 1 cut(s) 594
StyD4I CCNGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 271, 387
TaiI ACGT 1 cut(s) 290
TaqI TCGA 3 cut(s) 22, 408, 513
TasI AATT 3 cut(s) 42, 221, 232
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 2 cut(s) 36, 481
TseI GCWGC 4 cut(s) 47, 50, 335, 538
Tsp45I GTSAC 2 cut(s) 36, 481
TspDTI ATGAA 4 cut(s) 64, 81, 175, 362
TspRI CASTG 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 420
XceI RCATGY 1 cut(s) 510
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.