RLG00000008447

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018588
Physical Location & Seq
Reverse (-)
30749729 .. 30751886
2158 bp
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UTR
Exon/CDS
Intron
RLM00000008447

Sequence Viewer

Length: 591 bp
ATGAAGAATATTCAGATCAGGATTTGGGAAGAGGAGTGGGAGGAGGAATTGGATGAAGATTCCAATGATGATGATGTGGAGACGGTGGTGGAGAGGAAAATGAAGGTGGTGGTGGCTGATGAGGAGGAGGAGATAGATGAGTTTAATGATCCAACAACAAGAGAAGAATGCATGGAGGATCTGTTGCTTGTGTGCTGTGTGCTGTTAGATGGGATTGAGTTGTTTCGGATGCAGTGGTGTGGAGGTGATGGGGCAGCAAGTGTTGGCCAATTGATTCAAAGGGTAATGAGGAGGCTAGAGCTTTCGATGTGTTGGCTGATTCGTGATCTTACGGTGGAGGGACGCAAGTATGATGCCATGAAACGGCGCGGCAAAGCAGGGCCTCGCCAACTGAATGCCCCTAACTTTGAAATGATTTCTCGACTATTGGAACTTTTCCTTGCATATGAAGAGGATGTTAATGCCAAGTTGGTGATTCTGAAGGGGAAAGGAAGAGCATTTTGTGCTGCTGGTGATGTAGCAGTTGTGATTCGTGATATTAATGAAGGAAATTGGAGATTAGGTGCACATTTTGTGTTAGTGGTGATGTAG

Protein Analysis

197

Amino Acids

22.58

Weight (kDa)

4.55

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 128 - 189 2.2e-11 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018588)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0410201
rosa_laevigata RLG00000008447 RLG00000022499 RLG00000023832
rosa_multiflora Rmu_co8422681.1_g000001 Rmu_sc0007314.1_g000003 Rmu_sc0012324.1_g000008
rosa_roxburghii Rroxscaffold_5G00354990
rosa_samantha Rh4CG171400 Rh4DG005800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 369
AciI CCGC 1 cut(s) 369
AclWI GGATC 2 cut(s) 143, 186
AcoI YGGCCR 1 cut(s) 265
AcuI CTGAAG 1 cut(s) 500
AfiI CCNNNNNNNGG 1 cut(s) 363
AgsI TTSAA 2 cut(s) 278, 410
AluBI AGCT 1 cut(s) 301
AluI AGCT 1 cut(s) 301
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 1 cut(s) 74
Alw44I GTGCAC 1 cut(s) 564
AlwI GGATC 2 cut(s) 143, 186
AoxI GGCC 2 cut(s) 265, 380
ApaLI GTGCAC 1 cut(s) 564
ApeKI GCWGC 2 cut(s) 254, 506
AseI ATTAAT 1 cut(s) 540
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 3 cut(s) 257, 484, 524
BaeGI GKGCMC 1 cut(s) 568
BalI TGGCCA 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 568
BbvI GCAGC 2 cut(s) 266, 493
BccI CCATC 2 cut(s) 203, 242
BceAI ACGGC 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 1 cut(s) 296
BisI GCNGC 3 cut(s) 255, 370, 507
BlsI GCNGC 3 cut(s) 256, 371, 508
BmgT120I GGNCC 1 cut(s) 380
BmsI GCATC 2 cut(s) 219, 343
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 547, 577
Bsc4I CCNNNNNNNGG 1 cut(s) 363
BseGI GGATG 3 cut(s) 58, 234, 460
BseLI CCNNNNNNNGG 1 cut(s) 363
BseRI GAGGAG 6 cut(s) 47, 56, 137, 140, 143, 304
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 2 cut(s) 266, 493
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 2 cut(s) 267, 382
BsiHKAI GWGCWC 1 cut(s) 568
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 1 cut(s) 363
BsmAI GTCTC 1 cut(s) 74
BsmBI CGTCTC 1 cut(s) 74
BsmFI GGGAC 1 cut(s) 354
BsmI GAATGC 2 cut(s) 173, 400
BsnI GGCC 2 cut(s) 267, 382
Bsp1286I GDGCHC 1 cut(s) 568
Bsp143I GATC 4 cut(s) 15, 148, 178, 325
BspACI CCGC 1 cut(s) 369
BspANI GGCC 2 cut(s) 267, 382
BspFNI CGCG 1 cut(s) 369
BspPI GGATC 2 cut(s) 143, 186
BspQI GCTCTTC 1 cut(s) 487
BssMI GATC 4 cut(s) 15, 148, 178, 325
Bst4CI ACNGT 2 cut(s) 85, 334
Bst6I CTCTTC 3 cut(s) 24, 444, 487
BstAPI GCANNNNNTGC 1 cut(s) 503
BstF5I GGATG 3 cut(s) 58, 234, 460
BstFNI CGCG 1 cut(s) 369
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 4 cut(s) 18, 151, 181, 328
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 4 cut(s) 15, 148, 178, 325
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstSLI GKGCMC 1 cut(s) 568
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 2 cut(s) 266, 493
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 2 cut(s) 267, 382
BtsCI GGATG 3 cut(s) 58, 234, 460
BtsI GCAGTG 1 cut(s) 239
BtsIMutI CAGTG 1 cut(s) 239
CfoI GCGC 1 cut(s) 369
Cfr13I GGNCC 1 cut(s) 380
CseI GACGC 1 cut(s) 351
CviAII CATG 2 cut(s) 172, 358
CviJI RGCY 6 cut(s) 116, 267, 295, 301, 316, 382
CviKI_1 RGCY 6 cut(s) 116, 267, 295, 301, 316, 382
DpnI GATC 4 cut(s) 17, 150, 180, 327
DpnII GATC 4 cut(s) 15, 148, 178, 325
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 3 cut(s) 24, 444, 487
EarI CTCTTC 3 cut(s) 24, 444, 487
Eco57I CTGAAG 1 cut(s) 500
EcoO109I RGGNCCY 1 cut(s) 380
EcoT22I ATGCAT 1 cut(s) 173
Esp3I CGTCTC 1 cut(s) 74
FaeI CATG 2 cut(s) 175, 361
FaiI YATR 5 cut(s) 173, 351, 359, 445, 447
FaqI GGGAC 1 cut(s) 354
FatI CATG 2 cut(s) 171, 357
FauNDI CATATG 1 cut(s) 445
Fnu4HI GCNGC 3 cut(s) 255, 370, 507
FokI GGATG 3 cut(s) 65, 241, 467
Fsp4HI GCNGC 3 cut(s) 255, 370, 507
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 368
GluI GCNGC 3 cut(s) 255, 370, 507
HaeIII GGCC 2 cut(s) 267, 382
HgaI GACGC 1 cut(s) 351
HhaI GCGC 1 cut(s) 369
Hin1II CATG 2 cut(s) 175, 361
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HinfI GANTC 5 cut(s) 59, 274, 319, 475, 529
HphI GGTGA 3 cut(s) 257, 484, 524
Hpy166II GTNNAC 1 cut(s) 566
Hpy188I TCNGA 3 cut(s) 15, 228, 480
Hpy188III TCNNGA 4 cut(s) 19, 323, 420, 533
Hpy8I GTNNAC 1 cut(s) 566
HpyAV CCTTC 3 cut(s) 97, 475, 539
HpyCH4III ACNGT 2 cut(s) 85, 334
HpyCH4V TGCA 4 cut(s) 171, 232, 443, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
Hsp92II CATG 2 cut(s) 175, 361
HspAI GCGC 1 cut(s) 367
Kzo9I GATC 4 cut(s) 15, 148, 178, 325
LguI GCTCTTC 1 cut(s) 487
LpnPI CCDG 3 cut(s) 4, 363, 495
Lsp1109I GCAGC 2 cut(s) 266, 493
LweI GCATC 2 cut(s) 219, 343
MaeI CTAG 1 cut(s) 296
MalI GATC 4 cut(s) 17, 150, 180, 327
MboI GATC 4 cut(s) 15, 148, 178, 325
MboII GAAGA 6 cut(s) 16, 41, 68, 176, 461, 504
MfeI CAATTG 1 cut(s) 269
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 568
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 3 cut(s) 47, 269, 550
MluNI TGGCCA 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 176
Mox20I TGGCCA 1 cut(s) 267
Mph1103I ATGCAT 1 cut(s) 173
MscI TGGCCA 1 cut(s) 267
MseI TTAA 3 cut(s) 144, 459, 540
Msp20I TGGCCA 1 cut(s) 267
MunI CAATTG 1 cut(s) 269
Mva1269I GAATGC 2 cut(s) 173, 400
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 503
NdeI CATATG 1 cut(s) 445
NdeII GATC 4 cut(s) 15, 148, 178, 325
NlaIII CATG 2 cut(s) 175, 361
NsiI ATGCAT 1 cut(s) 173
PciSI GCTCTTC 1 cut(s) 487
PctI GAATGC 2 cut(s) 173, 400
PfeI GAWTC 5 cut(s) 59, 274, 319, 475, 529
PkrI GCNGC 3 cut(s) 256, 371, 508
PshBI ATTAAT 1 cut(s) 540
PspPI GGNCC 1 cut(s) 380
PsuI RGATCY 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 487
SaqAI TTAA 3 cut(s) 144, 459, 540
SatI GCNGC 3 cut(s) 255, 370, 507
Sau3AI GATC 4 cut(s) 15, 148, 178, 325
Sau96I GGNCC 1 cut(s) 380
SduI GDGCHC 1 cut(s) 568
SetI ASST 4 cut(s) 108, 247, 303, 565
SfaNI GCATC 2 cut(s) 219, 343
Sse9I AATT 3 cut(s) 47, 269, 550
SsiI CCGC 1 cut(s) 369
SspI AATATT 1 cut(s) 10
SspMI CTAG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 85, 334
TaqI TCGA 2 cut(s) 305, 421
TasI AATT 3 cut(s) 47, 269, 550
TauI GCSGC 1 cut(s) 372
TfiI GAWTC 5 cut(s) 59, 274, 319, 475, 529
Tru1I TTAA 3 cut(s) 144, 459, 540
Tru9I TTAA 3 cut(s) 144, 459, 540
TscAI CASTG 1 cut(s) 239
TseI GCWGC 2 cut(s) 254, 506
TspDTI ATGAA 6 cut(s) 17, 69, 116, 374, 462, 558
TspRI CASTG 1 cut(s) 239
VneI GTGCAC 1 cut(s) 564
VspI ATTAAT 1 cut(s) 540
XspI CTAG 1 cut(s) 296
Zsp2I ATGCAT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.