RLG00000008869

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014511
Physical Location & Seq
Forward (+)
41485823 .. 41487424
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008869

Sequence Viewer

Length: 1602 bp
ATGTCTCCCTTTCACTTCACCTTCCGCTCCGTCCTAAACCACCACCACCGCCACGTCAGCCGCAGAACCTTCGTGGACACCACCATAAACTGGGTCCGCGACCGCGGCCTCGACCACGTGGTCGAGCGGGAGAGAAACCTCCGGCCAATCATCAACATCAAGAACCTCATCAAATCAGAGCCCTCCAAATCCCTCCCCATTTCCCTCCTCACCAAATCGCGCCACTCCCTCATGATCCCCACGCGCCCCGTTGAGTTCGTCCGCCAATACCCCTCCGTTTTCGAGGAGTTCCTCCCCGCCGGCATCGCCGCCGTCCAGCCCCACGTCCGCCTCACCCCGCAACTCCTCGAGATCGACGCCAACGAGCAGTTGATGTATCAGAGCGTGACCTACACGCACCAAGCCGCCGATCGCCTCTTCAAGCTGCTAATGCTGGTGAGAAGCAACAAACTGCCGCTGAATATCATTGAGCTTTTCAAATGGGATTTGGGGCTTCCTCAAGATTACTTGGACACCATTGTGCCGGAGTTTCCGGATTACTTTAAGATTGTAGTGGGCAAAAATGGGCAGCCGGAGTTGGAGATGGTGTGTTGGAGTGATGAGCTGGCCACTTCGGTTATGGAGAAGAAAGCAAAACAGGGCTATGTCAAGGGAATGCCGCTAGCTTTTCCTATGCGGTTTTCGAGAGGTTTCGAGATGGATAAGAAGATGAAGAAGTGGATTGATGAGTGGCAGAAGTTGCCGTATGTGTCTCCTTATGAAAATGCAGCTCATCTGTCGTCTCAGAGTGACGAGTCGGATAAGTGGGCAGTTGCGGTTTTGCATGAGCTTCTGCATATTTTGGTTCCGAAGAAGACAGACAGGGAGAATGTTTTGGCTCTGGGAGAGTATTTGGGGATAAGATCGAGGTTTAAGCGGGCATTGCTTCACCATCCGGGAATATTGTATGTGTCGAGCAAGATTAGGACTTATACAGTTGTGTTGAGAGAAGGGTATAAGAGGGGTGCCATTATTGAAAATCATCCGCTGATGGACATAAGAAACCAGTATATTCACCTCATGAATGCTGTGAAGGAAGATGGTAAAACGATAAGTTCTAAGGAGAAAAAGAAGAAGAAGGGAATTGAAGATTCTAAAGGAGAGGATGTAGAAGAGGATGACGATGAAAATGAGGAAGAGCTGGAAGGGGAGCTGTGTGATTCGTCTGATGCTGAAGTGGAGCATGACAATGTTGACAGTGAAGATGGCGATGAAGAAGAGGATGAAGACGACGATGAAGAAGAGAGTATGACAAGCATTCGAATGAATGATGCTAATACTAGAGGAAGAAGAGCTAGGAAATCTAATTTTGATGTCATGACACCTTCCAGAAATGCTGGAAGAGGAAGTTCAGGTCAAAGAAGAGCTTGGAAGTCTGATAATACTGAGAGAAGGAGGTCCGGTGGACAATACCCTCTTAGGAGCAGGGATGAAGTCTCAGCAGAAACTTCTAGAAGAACACAAGCTGATGTTAGGCATATTGTCCACCAAAATTCAAGAGGAAGATCAAATCGCTCCAGAAGCAGGGGCAGTTCTTCACCAGACAAGAAGAATTATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

534

Amino Acids

61.67

Weight (kDa)

6.86

Isoelectric Point (pI)

57.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 32 - 357 2.4e-114 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71850
fragaria_vesca FvH4_4g08630 FvH4_4g08630
malus_domestica MD10G1045200.v1.1
prunus_persica Prupe.8G052100_v2.0.a1
pyrus_communis pycom10g03160
rosa_chinensis RchiOBHm_Chr4g0405141
rosa_laevigata RLG00000008869
rosa_multiflora Rmu_co8338777.1_g000001 Rmu_ssc0000255.1_g000039
rosa_roxburghii Rroxscaffold_5G00349370
rosa_rugosa Rorug04G0054000 Rorug04G0054100
rosa_samantha Rh4BG122500 Rh4CG136300 Rh4DG122700
rosa_wichuraiana Rw4G010430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 1004
AccB7I CCANNNNNTGG 1 cut(s) 90
AccBSI CCGCTC 2 cut(s) 27, 127
AccII CGCG 4 cut(s) 99, 105, 220, 244
AccIII TCCGGA 1 cut(s) 532
AclWI GGATC 1 cut(s) 229
AcoI YGGCCR 2 cut(s) 143, 606
AcsI RAATTY 1 cut(s) 1531
AcuI CTGAAG 1 cut(s) 1233
AcvI CACGTG 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 357
AfiI CCNNNNNNNGG 2 cut(s) 90, 1563
AgsI TTSAA 5 cut(s) 421, 478, 1016, 1127, 1536
AjiI CACGTC 2 cut(s) 55, 325
AleI CACNNNNGTG 1 cut(s) 518
Alw26I GTCTC 4 cut(s) 9, 756, 786, 1480
AlwI GGATC 1 cut(s) 229
Ama87I CYCGRG 1 cut(s) 347
Aor13HI TCCGGA 1 cut(s) 532
AoxI GGCC 3 cut(s) 106, 143, 606
ApeKI GCWGC 3 cut(s) 424, 568, 767
ApoI RAATTY 1 cut(s) 1531
AspLEI GCGC 2 cut(s) 222, 246
AspS9I GGNCC 2 cut(s) 94, 1437
AsuC2I CCSGG 1 cut(s) 936
AsuHPI GGTGA 7 cut(s) 10, 202, 325, 448, 920, 1046, 1569
AsuII TTCGAA 1 cut(s) 1300
AsuNHI GCTAGC 1 cut(s) 661
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 2 cut(s) 94, 1437
BalI TGGCCA 1 cut(s) 608
BanI GGYRCC 1 cut(s) 1004
BanII GRGCYC 1 cut(s) 183
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 2 cut(s) 860, 1272
BbvI GCAGC 3 cut(s) 411, 580, 779
BccI CCATC 6 cut(s) 577, 691, 939, 1024, 1073, 1238
BceAI ACGGC 2 cut(s) 296, 727
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BcnI CCSGG 1 cut(s) 936
BcoDI GTCTC 4 cut(s) 9, 756, 786, 1480
BfaI CTAG 4 cut(s) 662, 1320, 1335, 1491
BisI GCNGC 9 cut(s) 61, 106, 309, 405, 425, 455, 569, 659, 768
BlsI GCNGC 9 cut(s) 62, 107, 310, 406, 426, 456, 570, 660, 769
Bme1390I CCNGG 1 cut(s) 936
Bme18I GGWCC 2 cut(s) 94, 1437
BmeT110I CYCGRG 1 cut(s) 347
BmgBI CACGTC 2 cut(s) 55, 325
BmgT120I GGNCC 2 cut(s) 94, 1437
BmiI GGNNCC 3 cut(s) 95, 846, 1006
BmrFI CCNGG 1 cut(s) 936
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 3 cut(s) 312, 1198, 1300
BmtI GCTAGC 1 cut(s) 665
BmuI ACTGGG 1 cut(s) 100
BpiI GAAGAC 2 cut(s) 860, 1272
BpmI CTGGAG 1 cut(s) 1540
Bpu14I TTCGAA 1 cut(s) 1300
BpuEI CTTGAG 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 936
BsaAI YACGTR 1 cut(s) 118
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 103
BsaWI WCCGGW 2 cut(s) 532, 1439
BsaXI ACNNNNNCTCC 6 cut(s) 572, 602, 1131, 1161, 1426, 1456
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 1563
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 2 cut(s) 95, 1045
Bse3DI GCAATG 1 cut(s) 920
BseAI TCCGGA 1 cut(s) 532
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 6 cut(s) 931, 1021, 1150, 1162, 1267, 1474
BseLI CCNNNNNNNGG 2 cut(s) 90, 1563
BseMI GCAATG 1 cut(s) 920
BseMII CTCAG 3 cut(s) 797, 1416, 1491
BseNI ACTGG 2 cut(s) 95, 1045
BseRI GAGGAG 3 cut(s) 197, 299, 335
BseXI GCAGC 3 cut(s) 411, 580, 779
Bsh1236I CGCG 4 cut(s) 99, 105, 220, 244
Bsh1285I CGRYCG 2 cut(s) 103, 412
BshFI GGCC 3 cut(s) 108, 145, 608
BshNI GGYRCC 1 cut(s) 1004
BsiEI CGRYCG 2 cut(s) 103, 412
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 7 cut(s) 142, 300, 524, 533, 572, 935, 1440
BslI CCNNNNNNNGG 2 cut(s) 90, 1563
BsmAI GTCTC 4 cut(s) 9, 756, 786, 1480
BsmBI CGTCTC 1 cut(s) 786
BsmI GAATGC 3 cut(s) 660, 1069, 1296
BsnI GGCC 3 cut(s) 108, 145, 608
BsoBI CYCGRG 1 cut(s) 347
Bsp119I TTCGAA 1 cut(s) 1300
Bsp1286I GDGCHC 1 cut(s) 183
Bsp13I TCCGGA 1 cut(s) 532
Bsp143I GATC 5 cut(s) 234, 351, 409, 902, 1544
BspANI GGCC 3 cut(s) 108, 145, 608
BspCNI CTCAG 3 cut(s) 796, 1417, 1490
BspEI TCCGGA 1 cut(s) 532
BspFNI CGCG 4 cut(s) 99, 105, 220, 244
BspHI TCATGA 3 cut(s) 231, 1059, 1356
BspLI GGNNCC 3 cut(s) 95, 846, 1006
BspOI GCTAGC 1 cut(s) 665
BspPI GGATC 1 cut(s) 229
BspQI GCTCTTC 3 cut(s) 1170, 1324, 1396
BspT104I TTCGAA 1 cut(s) 1300
BspT107I GGYRCC 1 cut(s) 1004
BsrBI CCGCTC 2 cut(s) 27, 127
BsrDI GCAATG 1 cut(s) 920
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 2 cut(s) 95, 1045
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 5 cut(s) 234, 351, 409, 902, 1544
BssNI GRCGYC 1 cut(s) 357
Bst4CI ACNGT 2 cut(s) 976, 1238
Bst6I CTCTTC 8 cut(s) 422, 1146, 1170, 1251, 1275, 1324, 1375, 1396
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 739
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 1300
BstC8I GCNNGC 4 cut(s) 301, 606, 663, 918
BstDEI CTNAG 5 cut(s) 783, 1098, 1425, 1457, 1477
BstDSI CCRYGG 1 cut(s) 103
BstF5I GGATG 6 cut(s) 931, 1021, 1150, 1162, 1267, 1474
BstFNI CGCG 4 cut(s) 99, 105, 220, 244
BstHHI GCGC 2 cut(s) 222, 246
BstKTI GATC 5 cut(s) 237, 354, 412, 905, 1547
BstMAI GTCTC 4 cut(s) 9, 756, 786, 1480
BstMBI GATC 5 cut(s) 234, 351, 409, 902, 1544
BstMCI CGRYCG 2 cut(s) 103, 412
BstMWI GCNNNNNNNGC 7 cut(s) 57, 105, 305, 430, 739, 922, 1560
BstSCI CCNGG 1 cut(s) 934
BstUI CGCG 4 cut(s) 99, 105, 220, 244
BstV1I GCAGC 3 cut(s) 411, 580, 779
BstV2I GAAGAC 2 cut(s) 860, 1272
BsuRI GGCC 3 cut(s) 108, 145, 608
BtgI CCRYGG 1 cut(s) 103
BtgZI GCGATG 2 cut(s) 289, 1263
BtrI CACGTC 2 cut(s) 55, 325
BtsCI GGATG 6 cut(s) 931, 1021, 1150, 1162, 1267, 1474
BtsIMutI CAGTG 1 cut(s) 1243
Cac8I GCNNGC 4 cut(s) 301, 606, 663, 918
CciI TCATGA 3 cut(s) 231, 1059, 1356
CfoI GCGC 2 cut(s) 222, 246
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 2 cut(s) 94, 1437
Cfr42I CCGCGG 1 cut(s) 106
CseI GACGC 1 cut(s) 365
CviAII CATG 5 cut(s) 232, 824, 1060, 1223, 1357
DdeI CTNAG 5 cut(s) 783, 1098, 1425, 1457, 1477
DpnI GATC 5 cut(s) 236, 353, 411, 904, 1546
DpnII GATC 5 cut(s) 234, 351, 409, 902, 1544
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 2 cut(s) 143, 606
Eam1104I CTCTTC 8 cut(s) 422, 1146, 1170, 1251, 1275, 1324, 1375, 1396
EarI CTCTTC 8 cut(s) 422, 1146, 1170, 1251, 1275, 1324, 1375, 1396
EciI GGCGGA 2 cut(s) 251, 317
Eco24I GRGCYC 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 94, 1437
Eco57I CTGAAG 1 cut(s) 1233
Eco72I CACGTG 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 183
Esp3I CGTCTC 1 cut(s) 786
FaeI CATG 5 cut(s) 235, 827, 1063, 1226, 1360
FalI AAGNNNNNCTT 2 cut(s) 1390, 1422
FatI CATG 5 cut(s) 231, 823, 1059, 1222, 1356
FauI CCCGC 4 cut(s) 120, 304, 345, 909
Fnu4HI GCNGC 9 cut(s) 61, 106, 309, 405, 425, 455, 569, 659, 768
FokI GGATG 6 cut(s) 918, 1008, 1157, 1169, 1274, 1481
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 9 cut(s) 61, 106, 309, 405, 425, 455, 569, 659, 768
FspBI CTAG 4 cut(s) 662, 1320, 1335, 1491
GlaI GCGC 2 cut(s) 221, 245
GluI GCNGC 9 cut(s) 61, 106, 309, 405, 425, 455, 569, 659, 768
GsuI CTGGAG 1 cut(s) 1540
HaeIII GGCC 3 cut(s) 108, 145, 608
HapII CCGG 7 cut(s) 142, 300, 524, 533, 572, 935, 1440
HgaI GACGC 1 cut(s) 365
HhaI GCGC 2 cut(s) 222, 246
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 5 cut(s) 235, 827, 1063, 1226, 1360
Hin6I GCGC 2 cut(s) 220, 244
HinP1I GCGC 2 cut(s) 220, 244
HincII GTYRAC 1 cut(s) 1234
HindII GTYRAC 1 cut(s) 1234
HinfI GANTC 3 cut(s) 794, 1130, 1199
HpaII CCGG 7 cut(s) 142, 300, 524, 533, 572, 935, 1440
HphI GGTGA 7 cut(s) 10, 202, 325, 448, 920, 1046, 1569
Hpy166II GTNNAC 4 cut(s) 76, 1234, 1445, 1525
Hpy188I TCNGA 7 cut(s) 178, 381, 786, 799, 849, 1207, 1417
Hpy8I GTNNAC 4 cut(s) 76, 1234, 1445, 1525
Hpy99I CGWCG 2 cut(s) 359, 1274
HpyAV CCTTC 8 cut(s) 31, 79, 983, 1066, 1111, 1178, 1374, 1425
HpyCH4III ACNGT 2 cut(s) 976, 1238
HpyCH4IV ACGT 3 cut(s) 54, 117, 324
HpyCH4V TGCA 3 cut(s) 767, 823, 835
HpyF10VI GCNNNNNNNGC 7 cut(s) 57, 105, 305, 430, 739, 922, 1560
HpyF3I CTNAG 5 cut(s) 783, 1098, 1425, 1457, 1477
HpySE526I ACGT 3 cut(s) 54, 117, 324
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 5 cut(s) 235, 827, 1063, 1226, 1360
HspAI GCGC 2 cut(s) 220, 244
Kpn2I TCCGGA 1 cut(s) 532
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
KspI CCGCGG 1 cut(s) 106
Kzo9I GATC 5 cut(s) 234, 351, 409, 902, 1544
LguI GCTCTTC 3 cut(s) 1170, 1324, 1396
LmnI GCTCC 5 cut(s) 32, 1189, 1219, 1461, 1559
Lsp1109I GCAGC 3 cut(s) 411, 580, 779
LweI GCATC 3 cut(s) 312, 1198, 1300
MaeI CTAG 4 cut(s) 662, 1320, 1335, 1491
MaeII ACGT 3 cut(s) 54, 117, 324
MaeIII GTNAC 2 cut(s) 385, 788
MalI GATC 5 cut(s) 236, 353, 411, 904, 1546
MbiI CCGCTC 2 cut(s) 27, 127
MboI GATC 5 cut(s) 234, 351, 409, 902, 1544
MhlI GDGCHC 1 cut(s) 183
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 4 cut(s) 1122, 1345, 1531, 1591
MluNI TGGCCA 1 cut(s) 608
MlyI GAGTC 1 cut(s) 803
MmeI TCCRAC 3 cut(s) 558, 572, 777
Mox20I TGGCCA 1 cut(s) 608
MroI TCCGGA 1 cut(s) 532
MroNI GCCGGC 1 cut(s) 299
MscI TGGCCA 1 cut(s) 608
MseI TTAA 2 cut(s) 543, 912
MslI CAYNNNNRTG 3 cut(s) 518, 1227, 1301
Msp20I TGGCCA 1 cut(s) 608
MspA1I CMGCKG 3 cut(s) 105, 457, 1027
MspI CCGG 7 cut(s) 142, 300, 524, 533, 572, 935, 1440
MspR9I CCNGG 1 cut(s) 936
Mva1269I GAATGC 3 cut(s) 660, 1069, 1296
MvnI CGCG 4 cut(s) 99, 105, 220, 244
MwoI GCNNNNNNNGC 7 cut(s) 57, 105, 305, 430, 739, 922, 1560
NaeI GCCGGC 1 cut(s) 301
NciI CCSGG 1 cut(s) 936
NdeII GATC 5 cut(s) 234, 351, 409, 902, 1544
NgoMIV GCCGGC 1 cut(s) 299
NheI GCTAGC 1 cut(s) 661
NlaIII CATG 5 cut(s) 235, 827, 1063, 1226, 1360
NlaIV GGNNCC 3 cut(s) 95, 846, 1006
NmuCI GTSAC 2 cut(s) 385, 788
NspV TTCGAA 1 cut(s) 1300
OliI CACNNNNGTG 1 cut(s) 518
PaeR7I CTCGAG 1 cut(s) 347
PagI TCATGA 3 cut(s) 231, 1059, 1356
PciSI GCTCTTC 3 cut(s) 1170, 1324, 1396
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 3 cut(s) 660, 1069, 1296
PdiI GCCGGC 1 cut(s) 301
PfeI GAWTC 2 cut(s) 1130, 1199
PflMI CCANNNNNTGG 1 cut(s) 90
PfoI TCCNGGA 1 cut(s) 934
PkrI GCNGC 9 cut(s) 62, 107, 310, 406, 426, 456, 570, 660, 769
Ple19I CGATCG 1 cut(s) 412
PleI GAGTC 1 cut(s) 802
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 1 cut(s) 802
Ppu21I YACGTR 1 cut(s) 118
PspCI CACGTG 1 cut(s) 118
PspN4I GGNNCC 3 cut(s) 95, 846, 1006
PspPI GGNCC 2 cut(s) 94, 1437
PvuI CGATCG 1 cut(s) 412
RseI CAYNNNNRTG 3 cut(s) 518, 1227, 1301
SacII CCGCGG 1 cut(s) 106
SapI GCTCTTC 3 cut(s) 1170, 1324, 1396
SaqAI TTAA 2 cut(s) 543, 912
SatI GCNGC 9 cut(s) 61, 106, 309, 405, 425, 455, 569, 659, 768
Sau3AI GATC 5 cut(s) 234, 351, 409, 902, 1544
Sau96I GGNCC 2 cut(s) 94, 1437
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 1 cut(s) 936
SduI GDGCHC 1 cut(s) 183
SfaNI GCATC 3 cut(s) 312, 1198, 1300
Sfr274I CTCGAG 1 cut(s) 347
Sfr303I CCGCGG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 1300
SgrBI CCGCGG 1 cut(s) 106
SinI GGWCC 2 cut(s) 94, 1437
SlaI CTCGAG 1 cut(s) 347
SmiMI CAYNNNNRTG 3 cut(s) 518, 1227, 1301
SmlI CTYRAG 2 cut(s) 347, 498
SmoI CTYRAG 2 cut(s) 347, 498
Sse9I AATT 4 cut(s) 1122, 1345, 1531, 1591
SspI AATATT 1 cut(s) 942
SspMI CTAG 4 cut(s) 662, 1320, 1335, 1491
StyD4I CCNGG 1 cut(s) 934
TaaI ACNGT 2 cut(s) 976, 1238
TaiI ACGT 3 cut(s) 57, 120, 327
TasI AATT 4 cut(s) 1122, 1345, 1531, 1591
TauI GCSGC 6 cut(s) 63, 108, 311, 407, 457, 661
TfiI GAWTC 2 cut(s) 1130, 1199
Tru1I TTAA 2 cut(s) 543, 912
Tru9I TTAA 2 cut(s) 543, 912
TscAI CASTG 1 cut(s) 1243
TseFI GTSAC 2 cut(s) 385, 788
TseI GCWGC 3 cut(s) 424, 568, 767
Tsp45I GTSAC 2 cut(s) 385, 788
TspDTI ATGAA 9 cut(s) 725, 774, 1076, 1179, 1266, 1278, 1290, 1319, 1485
TspGWI ACGGA 2 cut(s) 19, 265
TspRI CASTG 1 cut(s) 1243
Van91I CCANNNNNTGG 1 cut(s) 90
VpaK11BI GGWCC 2 cut(s) 94, 1437
XapI RAATTY 1 cut(s) 1531
XbaI TCTAGA 1 cut(s) 1490
XcmI CCANNNNNNNNNTGG 1 cut(s) 616
XhoI CTCGAG 1 cut(s) 347
XspI CTAG 4 cut(s) 662, 1320, 1335, 1491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.