Rroxscaffold_5G00349370

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014511
Physical Location & Seq
Reverse (-)
22270377 .. 22275711
5335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00349370.1

Sequence Viewer

Length: 1605 bp
ATGTCTCCCTTTCACTTCACCTTCCGCTCCGTCCTAAACGCCCGCCACCACCACCGCCACGTCAGCCGCAGAACCTTCGTGGACACCACCATAAACTGGGTCCGCGACCGCGGCCTCGACCACGTGGTCGAGCGGGAGAGAAACCTCCGGCCACTCATCAACATCAAGAACCTCATCAAATCAGAGCCCTCCAAATCCCTCCCCATTTCCCTCCTCACCAAATCCCGCCACTCCCTCATGATCCCCACGCGCCCCGTCGAGTTCGTCCGCCAATACCCCTCCATTTTCGAGGAGTTCCTCCCCGCCGGCATCGCCGCCGTCCAGCCCCACGTCCGCCTCACCCCGCAAGTCCTCGACATCGACGCCAACGAGCAGTTGATGTATCAGAGCGTGACCTACACGCACCAAGCCGCCGATCGCCTCTTCAAGCTGCTAATGCTGGTGAGAAGCAATAAACTGCCGCTGAATCTCATTGAGCTTTTCAAATGGGATTTGGGGCTTCCTCAAGATTACGTGGACACCATTGTGCCGGAGTTTCCGGATTACTTTAAGATTGTAGTGGGCAAAAATGGGCAGCCGGAGTTGGAGATGGTGTGTTGGAGTGATGAGCTGGCCACTTCGGTTGTGGAGAAGAAAGCGAAACAGGGCTATGTCAAGGGAATGCCGCTTGCTTTTCCTATGCGGTTTTCGAGAGGTTTCGAGATGGATAAGAAGATGAAGAAGTGGATTGATGAGTGGCAGAAGTTGCCGTATGTGTCTCCTTATGAAAATGCAGCTCATCTGTCGTCTCAGAGTGACGAGTCGGATAAGTGGGCCGTTGCGGTTTTGCATGAGCTTCTGCATATTTTGGTTCCGAAGAAGACAGACAGGGAGAATGTTTTGGCTCTGGGAGAGTATTTGGGGATTAGATCGAGGTTTAAGCGGGCATTGCTTCACCATCCGGGTATATTGTATGTGTCGAGCAAGATTAGGACTTATACAGTTGTGTTGAGAGAAGGGTATAAGAGGGGTGCCATTATTGAAAATCATCCGCTGATGGACATAAGAAACCAGTATATTCACCTCATGAATGCTGTGAAGGAAGATGGTAAAACGATAAGTTCTAAGGAGAAAAAGAAGAAGAAGGGAATTGAAGATTCTAAAGGAGAGGATGTAGAAGAGGACGACGATGAAAATGAGGAAGAGCTGGAAGGGGAGTTGTGTGATTCGTCTGATGCTGAAGTGGAGCATGACAACGTTGACAGTGAAGACGATGAAGAAGAAGATGAAGACGATGATGAAGAAGAGAGTATGACAAGCATTCGAATGAATGATGCTAATACTAGAGGAAGAAGAGCTAGGAAATCTAATTTTGATGTCATGACACCTTCCAGAAATGCTGGAAGAGGAAGTTCAGGTCAAAGAAGAGCTTGGAAATCTGATAATACTGAGAGAAGGAGGTCCGGTGGACAATACCCTCTTAGGAGCGGGAATGAAGTCTCAGCAGAAACTTCTAGAAGAACACAAGCTGATGTTAGGCATAATGTCCACCAAAAGTCAAGAGAAAGATCAAATCGCTCCAGAAGCAGGGGGAGTTCTTCATCAGACAAGAAGAATTATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

534

Amino Acids

61.76

Weight (kDa)

7.06

Isoelectric Point (pI)

55.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 34 - 359 3e-114 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71850
fragaria_vesca FvH4_4g08630 FvH4_4g08630
malus_domestica MD10G1045200.v1.1
prunus_persica Prupe.8G052100_v2.0.a1
pyrus_communis pycom10g03160
rosa_chinensis RchiOBHm_Chr4g0405141
rosa_laevigata RLG00000008869
rosa_multiflora Rmu_co8338777.1_g000001 Rmu_ssc0000255.1_g000039
rosa_roxburghii Rroxscaffold_5G00349370
rosa_rugosa Rorug04G0054000 Rorug04G0054100
rosa_samantha Rh4BG122500 Rh4CG136300 Rh4DG122700
rosa_wichuraiana Rw4G010430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 1010
AccB7I CCANNNNNTGG 1 cut(s) 96
AccBSI CCGCTC 3 cut(s) 27, 133, 1467
AccII CGCG 3 cut(s) 105, 111, 250
AccIII TCCGGA 1 cut(s) 538
AclI AACGTT 1 cut(s) 1236
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 2 cut(s) 149, 612
AcuI CTGAAG 1 cut(s) 1239
AcvI CACGTG 1 cut(s) 124
AcyI GRCGYC 1 cut(s) 363
AfiI CCNNNNNNNGG 2 cut(s) 96, 1566
AgsI TTSAA 4 cut(s) 427, 484, 1022, 1133
AjiI CACGTC 2 cut(s) 61, 331
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 9 cut(s) 430, 478, 610, 776, 835, 1186, 1337, 1409, 1508
AluI AGCT 9 cut(s) 430, 478, 610, 776, 835, 1186, 1337, 1409, 1508
Alw26I GTCTC 4 cut(s) 9, 762, 792, 1483
AlwI GGATC 1 cut(s) 235
Aor13HI TCCGGA 1 cut(s) 538
AoxI GGCC 4 cut(s) 112, 149, 612, 813
ApeKI GCWGC 3 cut(s) 430, 574, 773
AspLEI GCGC 1 cut(s) 252
AspS9I GGNCC 3 cut(s) 100, 813, 1440
AsuC2I CCSGG 1 cut(s) 942
AsuHPI GGTGA 6 cut(s) 10, 208, 331, 454, 926, 1052
AsuII TTCGAA 1 cut(s) 1303
AvaII GGWCC 2 cut(s) 100, 1440
BalI TGGCCA 1 cut(s) 614
BanI GGYRCC 1 cut(s) 1010
BanII GRGCYC 1 cut(s) 189
BbrPI CACGTG 1 cut(s) 124
BbsI GAAGAC 3 cut(s) 866, 1254, 1275
BbvI GCAGC 3 cut(s) 417, 586, 785
BccI CCATC 5 cut(s) 583, 697, 945, 1030, 1079
BceAI ACGGC 3 cut(s) 302, 733, 800
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BcnI CCSGG 1 cut(s) 942
BcoDI GTCTC 4 cut(s) 9, 762, 792, 1483
BfaI CTAG 3 cut(s) 1323, 1338, 1494
BisI GCNGC 9 cut(s) 67, 112, 315, 411, 431, 461, 575, 665, 774
BlsI GCNGC 9 cut(s) 68, 113, 316, 412, 432, 462, 576, 666, 775
Bme1390I CCNGG 1 cut(s) 942
Bme18I GGWCC 2 cut(s) 100, 1440
BmgBI CACGTC 2 cut(s) 61, 331
BmgT120I GGNCC 3 cut(s) 100, 813, 1440
BmiI GGNNCC 3 cut(s) 101, 852, 1012
BmrFI CCNGG 1 cut(s) 942
BmrI ACTGGG 1 cut(s) 106
BmsI GCATC 3 cut(s) 318, 1204, 1303
BmuI ACTGGG 1 cut(s) 106
BpiI GAAGAC 3 cut(s) 866, 1254, 1275
BpmI CTGGAG 1 cut(s) 1543
Bpu14I TTCGAA 1 cut(s) 1303
BpuEI CTTGAG 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 942
BsaAI YACGTR 2 cut(s) 124, 514
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 109
BsaWI WCCGGW 2 cut(s) 538, 1442
BsaXI ACNNNNNCTCC 6 cut(s) 578, 608, 1137, 1167, 1429, 1459
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 1566
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 2 cut(s) 101, 1051
Bse3DI GCAATG 1 cut(s) 926
BseAI TCCGGA 1 cut(s) 538
BseDI CCNNGG 1 cut(s) 109
BseGI GGATG 3 cut(s) 937, 1027, 1156
BseLI CCNNNNNNNGG 2 cut(s) 96, 1566
BseMI GCAATG 1 cut(s) 926
BseMII CTCAG 3 cut(s) 803, 1419, 1494
BseNI ACTGG 2 cut(s) 101, 1051
BseRI GAGGAG 2 cut(s) 203, 305
BseXI GCAGC 3 cut(s) 417, 586, 785
Bsh1236I CGCG 3 cut(s) 105, 111, 250
Bsh1285I CGRYCG 2 cut(s) 109, 418
BshFI GGCC 4 cut(s) 114, 151, 614, 815
BshNI GGYRCC 1 cut(s) 1010
BsiEI CGRYCG 2 cut(s) 109, 418
BsiSI CCGG 7 cut(s) 148, 306, 530, 539, 578, 941, 1443
BslI CCNNNNNNNGG 2 cut(s) 96, 1566
BsmAI GTCTC 4 cut(s) 9, 762, 792, 1483
BsmBI CGTCTC 1 cut(s) 792
BsmI GAATGC 3 cut(s) 666, 1075, 1299
BsnI GGCC 4 cut(s) 114, 151, 614, 815
Bsp119I TTCGAA 1 cut(s) 1303
Bsp1286I GDGCHC 1 cut(s) 189
Bsp13I TCCGGA 1 cut(s) 538
Bsp143I GATC 4 cut(s) 240, 415, 908, 1547
BspANI GGCC 4 cut(s) 114, 151, 614, 815
BspCNI CTCAG 3 cut(s) 802, 1420, 1493
BspEI TCCGGA 1 cut(s) 538
BspFNI CGCG 3 cut(s) 105, 111, 250
BspHI TCATGA 3 cut(s) 237, 1065, 1359
BspLI GGNNCC 3 cut(s) 101, 852, 1012
BspPI GGATC 1 cut(s) 235
BspQI GCTCTTC 3 cut(s) 1176, 1327, 1399
BspT104I TTCGAA 1 cut(s) 1303
BspT107I GGYRCC 1 cut(s) 1010
BsrBI CCGCTC 3 cut(s) 27, 133, 1467
BsrDI GCAATG 1 cut(s) 926
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 2 cut(s) 101, 1051
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 4 cut(s) 240, 415, 908, 1547
BssNI GRCGYC 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 982, 1244
Bst6I CTCTTC 7 cut(s) 428, 1152, 1176, 1278, 1327, 1378, 1399
BstACI GRCGYC 1 cut(s) 363
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBAI YACGTR 2 cut(s) 124, 514
BstBI TTCGAA 1 cut(s) 1303
BstC8I GCNNGC 5 cut(s) 43, 307, 612, 669, 924
BstDEI CTNAG 5 cut(s) 789, 1104, 1428, 1460, 1480
BstDSI CCRYGG 1 cut(s) 109
BstF5I GGATG 3 cut(s) 937, 1027, 1156
BstFNI CGCG 3 cut(s) 105, 111, 250
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 4 cut(s) 243, 418, 911, 1550
BstMAI GTCTC 4 cut(s) 9, 762, 792, 1483
BstMBI GATC 4 cut(s) 240, 415, 908, 1547
BstMCI CGRYCG 2 cut(s) 109, 418
BstMWI GCNNNNNNNGC 7 cut(s) 63, 111, 311, 436, 745, 928, 1563
BstSCI CCNGG 1 cut(s) 940
BstUI CGCG 3 cut(s) 105, 111, 250
BstV1I GCAGC 3 cut(s) 417, 586, 785
BstV2I GAAGAC 3 cut(s) 866, 1254, 1275
BsuRI GGCC 4 cut(s) 114, 151, 614, 815
BtgI CCRYGG 1 cut(s) 109
BtgZI GCGATG 1 cut(s) 295
BtrI CACGTC 2 cut(s) 61, 331
BtsCI GGATG 3 cut(s) 937, 1027, 1156
BtsIMutI CAGTG 1 cut(s) 1249
Cac8I GCNNGC 5 cut(s) 43, 307, 612, 669, 924
CciI TCATGA 3 cut(s) 237, 1065, 1359
CfoI GCGC 1 cut(s) 252
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 3 cut(s) 100, 813, 1440
Cfr42I CCGCGG 1 cut(s) 112
CseI GACGC 1 cut(s) 371
CspCI CAANNNNNGTGG 2 cut(s) 604, 639
CviAII CATG 5 cut(s) 238, 830, 1066, 1229, 1360
DdeI CTNAG 5 cut(s) 789, 1104, 1428, 1460, 1480
DpnI GATC 4 cut(s) 242, 417, 910, 1549
DpnII GATC 4 cut(s) 240, 415, 908, 1547
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 2 cut(s) 149, 612
Eam1104I CTCTTC 7 cut(s) 428, 1152, 1176, 1278, 1327, 1378, 1399
EarI CTCTTC 7 cut(s) 428, 1152, 1176, 1278, 1327, 1378, 1399
EciI GGCGGA 2 cut(s) 257, 323
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 100, 1440
Eco57I CTGAAG 1 cut(s) 1239
Eco72I CACGTG 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 189
Esp3I CGTCTC 1 cut(s) 792
FaeI CATG 5 cut(s) 241, 833, 1069, 1232, 1363
FalI AAGNNNNNCTT 2 cut(s) 1393, 1425
FatI CATG 5 cut(s) 237, 829, 1065, 1228, 1359
FauI CCCGC 7 cut(s) 50, 126, 233, 310, 351, 915, 1460
Fnu4HI GCNGC 9 cut(s) 67, 112, 315, 411, 431, 461, 575, 665, 774
FokI GGATG 3 cut(s) 924, 1014, 1163
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 9 cut(s) 67, 112, 315, 411, 431, 461, 575, 665, 774
FspBI CTAG 3 cut(s) 1323, 1338, 1494
GlaI GCGC 1 cut(s) 251
GluI GCNGC 9 cut(s) 67, 112, 315, 411, 431, 461, 575, 665, 774
GsuI CTGGAG 1 cut(s) 1543
HaeIII GGCC 4 cut(s) 114, 151, 614, 815
HapII CCGG 7 cut(s) 148, 306, 530, 539, 578, 941, 1443
HgaI GACGC 1 cut(s) 371
HhaI GCGC 1 cut(s) 252
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 5 cut(s) 241, 833, 1069, 1232, 1363
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HincII GTYRAC 1 cut(s) 1240
HindII GTYRAC 1 cut(s) 1240
HinfI GANTC 4 cut(s) 466, 800, 1136, 1205
HpaII CCGG 7 cut(s) 148, 306, 530, 539, 578, 941, 1443
HphI GGTGA 6 cut(s) 10, 208, 331, 454, 926, 1052
Hpy166II GTNNAC 5 cut(s) 82, 517, 1240, 1448, 1528
Hpy188I TCNGA 8 cut(s) 184, 387, 792, 805, 855, 1213, 1420, 1585
Hpy8I GTNNAC 5 cut(s) 82, 517, 1240, 1448, 1528
Hpy99I CGWCG 3 cut(s) 260, 365, 1169
HpyAV CCTTC 8 cut(s) 31, 85, 989, 1072, 1117, 1184, 1377, 1428
HpyCH4III ACNGT 2 cut(s) 982, 1244
HpyCH4IV ACGT 5 cut(s) 60, 123, 330, 513, 1236
HpyCH4V TGCA 3 cut(s) 773, 829, 841
HpyF10VI GCNNNNNNNGC 7 cut(s) 63, 111, 311, 436, 745, 928, 1563
HpyF3I CTNAG 5 cut(s) 789, 1104, 1428, 1460, 1480
HpySE526I ACGT 5 cut(s) 60, 123, 330, 513, 1236
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 5 cut(s) 241, 833, 1069, 1232, 1363
HspAI GCGC 1 cut(s) 250
Kpn2I TCCGGA 1 cut(s) 538
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
KspI CCGCGG 1 cut(s) 112
Kzo9I GATC 4 cut(s) 240, 415, 908, 1547
LguI GCTCTTC 3 cut(s) 1176, 1327, 1399
LmnI GCTCC 4 cut(s) 32, 1225, 1464, 1562
Lsp1109I GCAGC 3 cut(s) 417, 586, 785
LweI GCATC 3 cut(s) 318, 1204, 1303
MaeI CTAG 3 cut(s) 1323, 1338, 1494
MaeII ACGT 5 cut(s) 60, 123, 330, 513, 1236
MaeIII GTNAC 2 cut(s) 391, 794
MalI GATC 4 cut(s) 242, 417, 910, 1549
MbiI CCGCTC 3 cut(s) 27, 133, 1467
MboI GATC 4 cut(s) 240, 415, 908, 1547
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 3 cut(s) 1128, 1348, 1594
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 1 cut(s) 809
MmeI TCCRAC 3 cut(s) 564, 578, 783
Mox20I TGGCCA 1 cut(s) 614
MroI TCCGGA 1 cut(s) 538
MroNI GCCGGC 1 cut(s) 305
MscI TGGCCA 1 cut(s) 614
MseI TTAA 2 cut(s) 549, 918
MslI CAYNNNNRTG 2 cut(s) 524, 1304
Msp20I TGGCCA 1 cut(s) 614
MspA1I CMGCKG 3 cut(s) 111, 463, 1033
MspI CCGG 7 cut(s) 148, 306, 530, 539, 578, 941, 1443
MspR9I CCNGG 1 cut(s) 942
Mva1269I GAATGC 3 cut(s) 666, 1075, 1299
MvnI CGCG 3 cut(s) 105, 111, 250
MwoI GCNNNNNNNGC 7 cut(s) 63, 111, 311, 436, 745, 928, 1563
NaeI GCCGGC 1 cut(s) 307
NciI CCSGG 1 cut(s) 942
NdeII GATC 4 cut(s) 240, 415, 908, 1547
NgoMIV GCCGGC 1 cut(s) 305
NlaIII CATG 5 cut(s) 241, 833, 1069, 1232, 1363
NlaIV GGNNCC 3 cut(s) 101, 852, 1012
NmuCI GTSAC 2 cut(s) 391, 794
NspV TTCGAA 1 cut(s) 1303
OliI CACNNNNGTG 1 cut(s) 524
PagI TCATGA 3 cut(s) 237, 1065, 1359
PciSI GCTCTTC 3 cut(s) 1176, 1327, 1399
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PctI GAATGC 3 cut(s) 666, 1075, 1299
PdiI GCCGGC 1 cut(s) 307
PfeI GAWTC 3 cut(s) 466, 1136, 1205
PflMI CCANNNNNTGG 1 cut(s) 96
PkrI GCNGC 9 cut(s) 68, 113, 316, 412, 432, 462, 576, 666, 775
Ple19I CGATCG 1 cut(s) 418
PleI GAGTC 1 cut(s) 808
PmaCI CACGTG 1 cut(s) 124
PmlI CACGTG 1 cut(s) 124
PpsI GAGTC 1 cut(s) 808
Ppu21I YACGTR 2 cut(s) 124, 514
Psp1406I AACGTT 1 cut(s) 1236
PspCI CACGTG 1 cut(s) 124
PspN4I GGNNCC 3 cut(s) 101, 852, 1012
PspPI GGNCC 3 cut(s) 100, 813, 1440
PvuI CGATCG 1 cut(s) 418
RseI CAYNNNNRTG 2 cut(s) 524, 1304
SacII CCGCGG 1 cut(s) 112
SapI GCTCTTC 3 cut(s) 1176, 1327, 1399
SaqAI TTAA 2 cut(s) 549, 918
SatI GCNGC 9 cut(s) 67, 112, 315, 411, 431, 461, 575, 665, 774
Sau3AI GATC 4 cut(s) 240, 415, 908, 1547
Sau96I GGNCC 3 cut(s) 100, 813, 1440
SchI GAGTC 1 cut(s) 809
ScrFI CCNGG 1 cut(s) 942
SduI GDGCHC 1 cut(s) 189
SfaNI GCATC 3 cut(s) 318, 1204, 1303
Sfr303I CCGCGG 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 1303
SgrBI CCGCGG 1 cut(s) 112
SinI GGWCC 2 cut(s) 100, 1440
SmiMI CAYNNNNRTG 2 cut(s) 524, 1304
SmlI CTYRAG 1 cut(s) 504
SmoI CTYRAG 1 cut(s) 504
Sse9I AATT 3 cut(s) 1128, 1348, 1594
SspMI CTAG 3 cut(s) 1323, 1338, 1494
StyD4I CCNGG 1 cut(s) 940
TaaI ACNGT 2 cut(s) 982, 1244
TaiI ACGT 5 cut(s) 63, 126, 333, 516, 1239
TasI AATT 3 cut(s) 1128, 1348, 1594
TauI GCSGC 6 cut(s) 69, 114, 317, 413, 463, 667
TfiI GAWTC 3 cut(s) 466, 1136, 1205
Tru1I TTAA 2 cut(s) 549, 918
Tru9I TTAA 2 cut(s) 549, 918
TscAI CASTG 1 cut(s) 1249
TseFI GTSAC 2 cut(s) 391, 794
TseI GCWGC 3 cut(s) 430, 574, 773
Tsp45I GTSAC 2 cut(s) 391, 794
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 1 cut(s) 1249
Van91I CCANNNNNTGG 1 cut(s) 96
VpaK11BI GGWCC 2 cut(s) 100, 1440
XbaI TCTAGA 1 cut(s) 1493
XcmI CCANNNNNNNNNTGG 1 cut(s) 622
XspI CTAG 3 cut(s) 1323, 1338, 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.