Rorug04G0054100

Abnormal spindle-like microcephaly-associated protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005182
Physical Location & Seq
Reverse (-)
8490293 .. 8541319
51027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0054100.1

Sequence Viewer

Length: 774 bp
ATGGTGAGGCCCAACCGCTACTTGCATGTTGTAGCCCTATGTTCAAGCTACGCTACGGTACAAGGAACTGAGATTGAGGCCAAGTTCGCATTAGGTTCAGTTGATCATATTGTAGCTCTTGGTACTGTTATGGAACACAATGACCCCTCCCAAGTTTGTCATGGTTATCCGCTAGGGGATAGTAATTTGCGTGTTTCTGTCTCTGTTGCAATTGAGGATAATGCCTTAGTTCCATTTCCTGTGGGTGATGAAATACGAACTGTTAGGCAGGCCATGGGGAGTTGGGTAGCATGGCCGAAAGAGCTTGTCATAATGTCACCTGTGAAGAGACCTGAGAAGAGGAGAAAAAAGAAAGTGGTACAAGAAGACGATCTCTCTGAGGATGAGTTTAGCCTAAAGTCATTGGCAGCTTCATTACCAAATTCATTGAGCATGTTGTGCATGTGGGGTGAGGTCGGATTCAAAGATGGGAAGGCTATCACCATTCAAATTGAGGCTGAAGTGTTTGGGCGTGTACGAAAAACATTTATACTAGGACAAGACGTGCGCAAAATTGCAACCATGAGAGAATTAACTGGTAACTGCATAGTCATATACCAGAGGTACCTCTATGATGTCCTGAGTCAGTATAAAATGTTGGATATGGTTGCTTTTATTGACCCTGCGATGATTGGTGCAATTGCATGGGTGTGGGAAAGGCCAGGAAAGATCCCAACATATAAAGGAGAGGCTGATATCTGCAAAACCTGGACAGATTTTCTTGCTGCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm CH IQ

Protein Analysis

257

Amino Acids

28.79

Weight (kDa)

6.45

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 33 - 105 8.8e-12 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 548
Acc65I GGTACC 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 603
AciI CCGC 2 cut(s) 16, 170
AclWI GGATC 1 cut(s) 703
AcoI YGGCCR 1 cut(s) 293
AcsI RAATTY 1 cut(s) 421
AcuI CTGAAG 1 cut(s) 519
AfaI GTAC 5 cut(s) 60, 124, 360, 516, 605
AgsI TTSAA 3 cut(s) 45, 463, 488
AjiI CACGTC 1 cut(s) 544
AjnI CCWGG 2 cut(s) 700, 746
AluBI AGCT 4 cut(s) 48, 116, 304, 410
AluI AGCT 4 cut(s) 48, 116, 304, 410
Alw26I GTCTC 2 cut(s) 205, 322
AlwI GGATC 1 cut(s) 703
AoxI GGCC 5 cut(s) 8, 78, 270, 293, 698
ApeKI GCWGC 2 cut(s) 407, 764
ApoI RAATTY 1 cut(s) 421
Asp718I GGTACC 1 cut(s) 603
AspLEI GCGC 1 cut(s) 549
AspS9I GGNCC 1 cut(s) 9
AsuHPI GGTGA 5 cut(s) 16, 257, 309, 461, 472
BanI GGYRCC 1 cut(s) 603
BbsI GAAGAC 1 cut(s) 372
BbvI GCAGC 2 cut(s) 419, 751
BccI CCATC 1 cut(s) 461
BciT130I CCWGG 2 cut(s) 702, 748
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 205, 322
BfaI CTAG 2 cut(s) 173, 533
BisI GCNGC 2 cut(s) 408, 765
BlsI GCNGC 2 cut(s) 409, 766
Bme1390I CCNGG 2 cut(s) 702, 748
BmgBI CACGTC 1 cut(s) 544
BmgT120I GGNCC 1 cut(s) 9
BmiI GGNNCC 1 cut(s) 605
BmrFI CCNGG 2 cut(s) 702, 748
BpiI GAAGAC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 580
BseBI CCWGG 2 cut(s) 702, 748
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 388
BseMII CTCAG 4 cut(s) 60, 324, 369, 611
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 355
BseXI GCAGC 2 cut(s) 419, 751
BshFI GGCC 5 cut(s) 10, 80, 272, 295, 700
BshNI GGYRCC 1 cut(s) 603
BsmAI GTCTC 2 cut(s) 205, 322
BsnI GGCC 5 cut(s) 10, 80, 272, 295, 700
Bso31I GGTCTC 1 cut(s) 322
Bsp143I GATC 3 cut(s) 103, 370, 708
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 2 cut(s) 16, 170
BspANI GGCC 5 cut(s) 10, 80, 272, 295, 700
BspCNI CTCAG 4 cut(s) 61, 325, 370, 612
BspLI GGNNCC 1 cut(s) 605
BspPI GGATC 1 cut(s) 703
BspT107I GGYRCC 1 cut(s) 603
BspTNI GGTCTC 1 cut(s) 322
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 3 cut(s) 103, 370, 708
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 2 cut(s) 702, 748
Bst4CI ACNGT 3 cut(s) 58, 127, 262
Bst6I CTCTTC 2 cut(s) 320, 332
BstAPI GCANNNNNTGC 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 5 cut(s) 69, 226, 333, 378, 620
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 388
BstHHI GCGC 1 cut(s) 549
BstKTI GATC 3 cut(s) 106, 373, 711
BstMAI GTCTC 2 cut(s) 205, 322
BstMBI GATC 3 cut(s) 103, 370, 708
BstMWI GCNNNNNNNGC 3 cut(s) 86, 301, 438
BstNI CCWGG 2 cut(s) 702, 748
BstNSI RCATGY 3 cut(s) 29, 436, 445
BstSCI CCNGG 2 cut(s) 700, 746
BstV1I GCAGC 2 cut(s) 419, 751
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 1 cut(s) 708
BstYI RGATCY 1 cut(s) 708
BsuRI GGCC 5 cut(s) 10, 80, 272, 295, 700
BtgI CCRYGG 1 cut(s) 273
BtgZI GCGATG 1 cut(s) 680
BtrI CACGTC 1 cut(s) 544
BtsCI GGATG 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 270
CfoI GCGC 1 cut(s) 549
Cfr13I GGNCC 1 cut(s) 9
Csp6I GTAC 5 cut(s) 59, 123, 359, 515, 604
CviAII CATG 8 cut(s) 26, 161, 274, 291, 433, 442, 562, 684
CviQI GTAC 5 cut(s) 59, 123, 359, 515, 604
DdeI CTNAG 5 cut(s) 69, 226, 333, 378, 620
DpnI GATC 3 cut(s) 105, 372, 710
DpnII GATC 3 cut(s) 103, 370, 708
EaeI YGGCCR 1 cut(s) 293
Eam1104I CTCTTC 2 cut(s) 320, 332
EarI CTCTTC 2 cut(s) 320, 332
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 322
Eco32I GATATC 1 cut(s) 736
Eco57I CTGAAG 1 cut(s) 519
EcoRII CCWGG 2 cut(s) 700, 746
EcoRV GATATC 1 cut(s) 736
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 8 cut(s) 29, 164, 277, 294, 436, 445, 565, 687
FatI CATG 8 cut(s) 25, 160, 273, 290, 432, 441, 561, 683
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 2 cut(s) 408, 765
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 2 cut(s) 408, 765
FspBI CTAG 2 cut(s) 173, 533
FspI TGCGCA 1 cut(s) 548
GlaI GCGC 1 cut(s) 548
GluI GCNGC 2 cut(s) 408, 765
HaeIII GGCC 5 cut(s) 10, 80, 272, 295, 700
HhaI GCGC 1 cut(s) 549
Hin1II CATG 8 cut(s) 29, 164, 277, 294, 436, 445, 565, 687
Hin6I GCGC 1 cut(s) 547
HinP1I GCGC 1 cut(s) 547
HinfI GANTC 2 cut(s) 459, 622
HphI GGTGA 5 cut(s) 16, 257, 309, 461, 472
Hpy166II GTNNAC 1 cut(s) 515
Hpy188I TCNGA 2 cut(s) 379, 458
Hpy188III TCNNGA 1 cut(s) 619
Hpy8I GTNNAC 1 cut(s) 515
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 3 cut(s) 58, 127, 262
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 8 cut(s) 25, 209, 441, 557, 585, 677, 683, 741
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 301, 438
HpyF3I CTNAG 5 cut(s) 69, 226, 333, 378, 620
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 8 cut(s) 29, 164, 277, 294, 436, 445, 565, 687
HspAI GCGC 1 cut(s) 547
KpnI GGTACC 1 cut(s) 607
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 3 cut(s) 103, 370, 708
Lsp1109I GCAGC 2 cut(s) 419, 751
MaeI CTAG 2 cut(s) 173, 533
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 2 cut(s) 315, 578
MalI GATC 3 cut(s) 105, 372, 710
MboI GATC 3 cut(s) 103, 370, 708
MboII GAAGA 3 cut(s) 337, 349, 377
MfeI CAATTG 2 cut(s) 210, 678
MflI RGATCY 1 cut(s) 708
MluCI AATT 7 cut(s) 184, 210, 421, 489, 552, 569, 678
MlyI GAGTC 1 cut(s) 631
MmeI TCCRAC 2 cut(s) 436, 618
MseI TTAA 1 cut(s) 572
MslI CAYNNNNRTG 1 cut(s) 688
MspR9I CCNGG 2 cut(s) 702, 748
MunI CAATTG 2 cut(s) 210, 678
MvaI CCWGG 2 cut(s) 702, 748
MwoI GCNNNNNNNGC 3 cut(s) 86, 301, 438
NcoI CCATGG 1 cut(s) 273
NdeII GATC 3 cut(s) 103, 370, 708
NlaIII CATG 8 cut(s) 29, 164, 277, 294, 436, 445, 565, 687
NlaIV GGNNCC 1 cut(s) 605
NmuCI GTSAC 1 cut(s) 315
NsbI TGCGCA 1 cut(s) 548
NspI RCATGY 3 cut(s) 29, 436, 445
PfeI GAWTC 1 cut(s) 459
PkrI GCNGC 2 cut(s) 409, 766
PleI GAGTC 1 cut(s) 630
PpsI GAGTC 1 cut(s) 630
Psp6I CCWGG 2 cut(s) 700, 746
PspGI CCWGG 2 cut(s) 700, 746
PspN4I GGNNCC 1 cut(s) 605
PspPI GGNCC 1 cut(s) 9
PsuI RGATCY 1 cut(s) 708
RsaI GTAC 5 cut(s) 60, 124, 360, 516, 605
RsaNI GTAC 5 cut(s) 59, 123, 359, 515, 604
RseI CAYNNNNRTG 1 cut(s) 688
SaqAI TTAA 1 cut(s) 572
SatI GCNGC 2 cut(s) 408, 765
Sau3AI GATC 3 cut(s) 103, 370, 708
Sau96I GGNCC 1 cut(s) 9
SchI GAGTC 1 cut(s) 631
ScrFI CCNGG 2 cut(s) 702, 748
SmiMI CAYNNNNRTG 1 cut(s) 688
Sse9I AATT 7 cut(s) 184, 210, 421, 489, 552, 569, 678
SsiI CCGC 2 cut(s) 16, 170
SspMI CTAG 2 cut(s) 173, 533
StyD4I CCNGG 2 cut(s) 700, 746
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 3 cut(s) 58, 127, 262
TaiI ACGT 1 cut(s) 546
TasI AATT 7 cut(s) 184, 210, 421, 489, 552, 569, 678
TfiI GAWTC 1 cut(s) 459
Tru1I TTAA 1 cut(s) 572
Tru9I TTAA 1 cut(s) 572
TseFI GTSAC 1 cut(s) 315
TseI GCWGC 2 cut(s) 407, 764
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 3 cut(s) 264, 402, 414
XapI RAATTY 1 cut(s) 421
XceI RCATGY 3 cut(s) 29, 436, 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XspI CTAG 2 cut(s) 173, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.