Rroxscaffold_2G00085360

Abnormal spindle-like microcephaly-associated protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005182
Physical Location & Seq
Forward (+)
7693480 .. 7695593
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00085360.1

Sequence Viewer

Length: 549 bp
ATGGGAACGATCGGCCGTGCATCGCCGGAAAAGTCGTTTTCCGCCGGGTCGGATCGAAAGGATCCGGGGTGGCTTCGATCCGTACTTGGAGGCAGCGGCGGGGTACGGCGACCCCGCAGGGAGGCTCGGCGTGAGGCGGCGATACCGGGGAGGCCGGGGAAATGGGTGTATATGGTTATAGATCTTCAGTTCGTTGAATATGCTTGCGGACAAGAGGATGTAGGAAATGGTATTCCACTAGTCTTCGCACCTAGTATACAAGACAATGAAGAAGATGGTGGACTTATTGTAAATGATGAAGATGTGAGAGCTGGTGTTGTTTTACATGATGATGGAATGATGATTGTGGAAGATGATATTACCGATGGGGACAAGGAACCTACCATTCCATTGCTTTGGAATATGTTTGTTCATTTGCAGCTGCCTCTTTTGGTCAAGAAAGCAACATTAGCCGATGACACATGCAAAATTCAAGAAACTATGGATGGCTCCATTAATGCCGATTCTCCTCCAATACTTTCGGGCTTTCATGATCAAGCTCGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm CH IQ

Protein Analysis

182

Amino Acids

19.82

Weight (kDa)

4.65

Isoelectric Point (pI)

55.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 256
AciI CCGC 6 cut(s) 42, 96, 99, 115, 137, 207
AclWI GGATC 4 cut(s) 56, 60, 69, 72
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 1 cut(s) 468
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 2 cut(s) 84, 105
AfiI CCNNNNNNNGG 1 cut(s) 121
AgsI TTSAA 2 cut(s) 197, 473
AhlI ACTAGT 1 cut(s) 238
AluBI AGCT 3 cut(s) 311, 421, 539
AluI AGCT 3 cut(s) 311, 421, 539
AlwI GGATC 4 cut(s) 56, 60, 69, 72
AoxI GGCC 2 cut(s) 13, 152
ApeKI GCWGC 3 cut(s) 93, 418, 421
ApoI RAATTY 1 cut(s) 468
AseI ATTAAT 1 cut(s) 495
AsuC2I CCSGG 4 cut(s) 46, 66, 147, 156
BamHI GGATCC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 235
BbvI GCAGC 3 cut(s) 105, 408, 430
BccI CCATC 4 cut(s) 269, 326, 359, 479
BceAI ACGGC 1 cut(s) 122
BclI TGATCA 1 cut(s) 532
BcnI CCSGG 4 cut(s) 46, 66, 147, 156
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 2 cut(s) 239, 252
BglII AGATCT 1 cut(s) 181
BisI GCNGC 5 cut(s) 94, 97, 138, 419, 422
BlsI GCNGC 5 cut(s) 95, 98, 139, 420, 423
Bme1390I CCNGG 4 cut(s) 46, 66, 147, 156
BmiI GGNNCC 3 cut(s) 63, 378, 490
BmrFI CCNGG 4 cut(s) 46, 66, 147, 156
BmsI GCATC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 235
BpuMI CCSGG 4 cut(s) 46, 66, 147, 156
BsaJI CCNNGG 3 cut(s) 65, 146, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse3DI GCAATG 1 cut(s) 389
BseDI CCNNGG 3 cut(s) 65, 146, 155
BseGI GGATG 2 cut(s) 223, 490
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMI GCAATG 1 cut(s) 389
BseRI GAGGAG 1 cut(s) 498
BseX3I CGGCCG 1 cut(s) 13
BseXI GCAGC 3 cut(s) 105, 408, 430
Bsh1285I CGRYCG 2 cut(s) 12, 16
BshFI GGCC 2 cut(s) 15, 154
BsiEI CGRYCG 2 cut(s) 12, 16
BsiSI CCGG 5 cut(s) 26, 45, 65, 146, 155
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 2 cut(s) 15, 154
Bsp143I GATC 7 cut(s) 9, 52, 61, 77, 181, 532, 542
BspACI CCGC 6 cut(s) 42, 96, 99, 115, 137, 207
BspANI GGCC 2 cut(s) 15, 154
BspHI TCATGA 2 cut(s) 529, 545
BspLI GGNNCC 3 cut(s) 63, 378, 490
BspPI GGATC 4 cut(s) 56, 60, 69, 72
BsrDI GCAATG 1 cut(s) 389
BssECI CCNNGG 3 cut(s) 65, 146, 155
BssMI GATC 7 cut(s) 9, 52, 61, 77, 181, 532, 542
BssNAI GTATAC 1 cut(s) 257
Bst1107I GTATAC 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 205
BstF5I GGATG 2 cut(s) 223, 490
BstKTI GATC 7 cut(s) 12, 55, 64, 80, 184, 535, 545
BstMBI GATC 7 cut(s) 9, 52, 61, 77, 181, 532, 542
BstMCI CGRYCG 2 cut(s) 12, 16
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNSI RCATGY 1 cut(s) 465
BstSCI CCNGG 4 cut(s) 44, 64, 145, 154
BstV1I GCAGC 3 cut(s) 105, 408, 430
BstV2I GAAGAC 1 cut(s) 235
BstX2I RGATCY 2 cut(s) 61, 181
BstXI CCANNNNNNTGG 1 cut(s) 396
BstYI RGATCY 2 cut(s) 61, 181
BstZ17I GTATAC 1 cut(s) 257
BstZI CGGCCG 1 cut(s) 13
BsuRI GGCC 2 cut(s) 15, 154
BtgZI GCGATG 1 cut(s) 6
BtsCI GGATG 2 cut(s) 223, 490
Cac8I GCNNGC 1 cut(s) 205
CciI TCATGA 2 cut(s) 529, 545
Csp6I GTAC 2 cut(s) 83, 104
CviAII CATG 4 cut(s) 326, 462, 530, 546
CviQI GTAC 2 cut(s) 83, 104
DpnI GATC 7 cut(s) 11, 54, 63, 79, 183, 534, 544
DpnII GATC 7 cut(s) 9, 52, 61, 77, 181, 532, 542
EaeI YGGCCR 1 cut(s) 13
EagI CGGCCG 1 cut(s) 13
EciI GGCGGA 1 cut(s) 31
EclXI CGGCCG 1 cut(s) 13
Eco52I CGGCCG 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 170
FaeI CATG 4 cut(s) 329, 465, 533, 549
FaqI GGGAC 1 cut(s) 383
FatI CATG 4 cut(s) 325, 461, 529, 545
FauI CCCGC 2 cut(s) 92, 122
FbaI TGATCA 1 cut(s) 532
FblI GTMKAC 1 cut(s) 256
Fnu4HI GCNGC 5 cut(s) 94, 97, 138, 419, 422
FokI GGATG 2 cut(s) 230, 497
Fsp4HI GCNGC 5 cut(s) 94, 97, 138, 419, 422
FspBI CTAG 2 cut(s) 239, 252
GluI GCNGC 5 cut(s) 94, 97, 138, 419, 422
HaeIII GGCC 2 cut(s) 15, 154
HapII CCGG 5 cut(s) 26, 45, 65, 146, 155
Hin1II CATG 4 cut(s) 329, 465, 533, 549
HinfI GANTC 1 cut(s) 503
HpaII CCGG 5 cut(s) 26, 45, 65, 146, 155
Hpy166II GTNNAC 2 cut(s) 257, 281
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 4 cut(s) 436, 473, 530, 546
Hpy8I GTNNAC 2 cut(s) 257, 281
HpyCH4V TGCA 3 cut(s) 20, 418, 465
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
Hsp92II CATG 4 cut(s) 329, 465, 533, 549
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 7 cut(s) 9, 52, 61, 77, 181, 532, 542
LmnI GCTCC 1 cut(s) 494
LpnPI CCDG 7 cut(s) 39, 58, 78, 103, 159, 168, 297
Lsp1109I GCAGC 3 cut(s) 105, 408, 430
LweI GCATC 1 cut(s) 29
MaeI CTAG 2 cut(s) 239, 252
MalI GATC 7 cut(s) 11, 54, 63, 79, 183, 534, 544
MboI GATC 7 cut(s) 9, 52, 61, 77, 181, 532, 542
MboII GAAGA 6 cut(s) 176, 235, 281, 284, 311, 362
MflI RGATCY 2 cut(s) 61, 181
MluCI AATT 1 cut(s) 468
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 7 cut(s) 83, 115, 127, 144, 208, 435, 519
MseI TTAA 1 cut(s) 495
MslI CAYNNNNRTG 1 cut(s) 330
MspA1I CMGCKG 2 cut(s) 96, 421
MspI CCGG 5 cut(s) 26, 45, 65, 146, 155
MspR9I CCNGG 4 cut(s) 46, 66, 147, 156
MwoI GCNNNNNNNGC 1 cut(s) 449
NciI CCSGG 4 cut(s) 46, 66, 147, 156
NdeII GATC 7 cut(s) 9, 52, 61, 77, 181, 532, 542
NlaIII CATG 4 cut(s) 329, 465, 533, 549
NlaIV GGNNCC 3 cut(s) 63, 378, 490
NmeAIII GCCGAG 1 cut(s) 106
NspI RCATGY 1 cut(s) 465
PagI TCATGA 2 cut(s) 529, 545
PfeI GAWTC 1 cut(s) 503
PkrI GCNGC 5 cut(s) 95, 98, 139, 420, 423
Ple19I CGATCG 1 cut(s) 12
PshBI ATTAAT 1 cut(s) 495
PspN4I GGNNCC 3 cut(s) 63, 378, 490
PsuI RGATCY 2 cut(s) 61, 181
PvuI CGATCG 1 cut(s) 12
PvuII CAGCTG 1 cut(s) 421
RsaI GTAC 2 cut(s) 84, 105
RsaNI GTAC 2 cut(s) 83, 104
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 5 cut(s) 94, 97, 138, 419, 422
Sau3AI GATC 7 cut(s) 9, 52, 61, 77, 181, 532, 542
ScrFI CCNGG 4 cut(s) 46, 66, 147, 156
SetI ASST 5 cut(s) 253, 313, 382, 423, 541
SfaNI GCATC 1 cut(s) 29
SmiMI CAYNNNNRTG 1 cut(s) 330
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 1 cut(s) 468
SsiI CCGC 6 cut(s) 42, 96, 99, 115, 137, 207
SspMI CTAG 2 cut(s) 239, 252
StyD4I CCNGG 4 cut(s) 44, 64, 145, 154
TaqI TCGA 3 cut(s) 55, 76, 541
TasI AATT 1 cut(s) 468
TauI GCSGC 2 cut(s) 99, 140
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TseI GCWGC 3 cut(s) 93, 418, 421
TspDTI ATGAA 4 cut(s) 282, 312, 401, 518
TspGWI ACGGA 1 cut(s) 70
VspI ATTAAT 1 cut(s) 495
XapI RAATTY 1 cut(s) 468
XceI RCATGY 1 cut(s) 465
XmiI GTMKAC 1 cut(s) 256
XspI CTAG 2 cut(s) 239, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.