RLG00000012253

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016264
Physical Location & Seq
Forward (+)
16773615 .. 16774531
917 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012253

Sequence Viewer

Length: 666 bp
ATGACTGACTTTTCCAAAAACTGGGAAAAACTGTCTGGTGACAATCTCTGGAGTGCTCTTAAAGATCCTCTCCACGTCGATCTCCGCCGCTATATCATTCATTACGGTGAAAGGGCTGGAGCTATTGACGATGCCTTCATTGATGGCAAGCTCGTTTCCCCGATAAATCCCCAGTCCATCGGATATCCTCGATATGCAAGGAAAGATTTATTCGATAAAGTGGGATTGGTGAAAGGCAATTATCGACCCACGTACATAGTGGAGAAATATGTGTATGGAGCACCAAAACTGCGATCTCGCCCATCCTACCTGTTGGCTTACGTTGCGGTGTCAGACAATGAAGGAACTAAAGCCTTGGGGAGGAGGGACATTCTGATTGCCTGGAGAGGAACCAAGTTGGAGGCTGAGGTTGCTGTCGAACAAAACACCGAACTACGCCCGGCTCCTAAAATTTTTGGAAACCAGTATGCCGGCGCTATGGTGCACGGCGGTTGGTATGATTATTACACTAATACTGACGTTAGGACCCCCAAGAACCAACGAACTAGTTGCCAAGATCAGGTTGGAGAGGCAGTTAGAAAACTGATAGATCAATACGGTAACGAGGGTCAAGAACTCAGCATTACTATAACTGGCCGCGGCACTGGTGCTGCATTTGCAATTTAA

Protein Analysis

222

Amino Acids

24.83

Weight (kDa)

8.98

Isoelectric Point (pI)

25.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 126 - 221 9e-09 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016264)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22960 FvH4_2g22990
rosa_chinensis RchiOBHm_Chr6g0289981
rosa_laevigata RLG00000012253 RLG00000012254 RLG00000012255
rosa_multiflora Rmu_sc0002902.1_g000066 Rmu_sc0003927.1_g000011
rosa_roxburghii Rroxscaffold_7G00177450
rosa_rugosa Rorug06G0208200
rosa_samantha Rh6AG319600 Rh6BG326700 Rh6CG332500 Rh6DG318400
rosa_wichuraiana Rw6G027580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 21
AccII CGCG 1 cut(s) 639
AciI CCGC 6 cut(s) 85, 88, 326, 489, 637, 639
AclWI GGATC 1 cut(s) 59
AcoI YGGCCR 1 cut(s) 634
AcsI RAATTY 1 cut(s) 450
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 3 cut(s) 21, 360, 559
AhlI ACTAGT 1 cut(s) 545
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 1 cut(s) 380
AluBI AGCT 2 cut(s) 122, 151
AluI AGCT 2 cut(s) 122, 151
Alw21I GWGCWC 3 cut(s) 58, 283, 486
Alw44I GTGCAC 1 cut(s) 482
AlwI GGATC 1 cut(s) 59
AoxI GGCC 1 cut(s) 634
ApaLI GTGCAC 1 cut(s) 482
ApeKI GCWGC 1 cut(s) 650
ApoI RAATTY 1 cut(s) 450
AspLEI GCGC 1 cut(s) 476
AspS9I GGNCC 1 cut(s) 525
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 3 cut(s) 50, 119, 241
AvaII GGWCC 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 486
Bbv12I GWGCWC 3 cut(s) 58, 283, 486
BbvCI CCTCAGC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 637
BccI CCATC 3 cut(s) 137, 185, 310
BceAI ACGGC 1 cut(s) 502
BcgI CGANNNNNNTGC 2 cut(s) 531, 565
BciT130I CCWGG 1 cut(s) 382
BcnI CCSGG 1 cut(s) 440
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 1 cut(s) 546
BfoI RGCGCY 1 cut(s) 477
BisI GCNGC 4 cut(s) 88, 637, 640, 651
BlsI GCNGC 4 cut(s) 89, 638, 641, 652
Bme1390I CCNGG 2 cut(s) 382, 440
Bme18I GGWCC 1 cut(s) 525
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 1 cut(s) 525
BmiI GGNNCC 3 cut(s) 391, 444, 527
BmrFI CCNGG 2 cut(s) 382, 440
BmrI ACTGGG 2 cut(s) 31, 166
BmsI GCATC 1 cut(s) 121
BmuI ACTGGG 2 cut(s) 31, 166
BpmI CTGGAG 3 cut(s) 70, 138, 403
Bpu10I CCTNAGC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 440
BsaAI YACGTR 1 cut(s) 252
BsaJI CCNNGG 2 cut(s) 354, 637
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 360, 559
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 5 cut(s) 26, 172, 463, 637, 649
BseBI CCWGG 1 cut(s) 382
BseDI CCNNGG 2 cut(s) 354, 637
BseGI GGATG 1 cut(s) 302
BseLI CCNNNNNNNGG 3 cut(s) 21, 360, 559
BseMII CTCAG 2 cut(s) 396, 631
BseNI ACTGG 5 cut(s) 26, 172, 463, 637, 649
BseRI GAGGAG 1 cut(s) 376
BseSI GKGCMC 1 cut(s) 486
BseXI GCAGC 1 cut(s) 637
Bsh1236I CGCG 1 cut(s) 639
BshFI GGCC 1 cut(s) 636
BsiHKAI GWGCWC 3 cut(s) 58, 283, 486
BsiSI CCGG 2 cut(s) 440, 471
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 3 cut(s) 21, 360, 559
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 1 cut(s) 636
Bsp1286I GDGCHC 3 cut(s) 58, 283, 486
Bsp143I GATC 5 cut(s) 64, 79, 293, 556, 589
BspACI CCGC 6 cut(s) 85, 88, 326, 489, 637, 639
BspANI GGCC 1 cut(s) 636
BspCNI CTCAG 2 cut(s) 397, 630
BspFNI CGCG 1 cut(s) 639
BspLI GGNNCC 3 cut(s) 391, 444, 527
BspPI GGATC 1 cut(s) 59
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 5 cut(s) 26, 172, 463, 637, 649
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 2 cut(s) 354, 637
BssMI GATC 5 cut(s) 64, 79, 293, 556, 589
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 382
Bst4CI ACNGT 3 cut(s) 33, 107, 599
BstBAI YACGTR 1 cut(s) 252
BstC8I GCNNGC 2 cut(s) 149, 472
BstDEI CTNAG 2 cut(s) 405, 617
BstDSI CCRYGG 1 cut(s) 637
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 1 cut(s) 302
BstFNI CGCG 1 cut(s) 639
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 5 cut(s) 67, 82, 296, 559, 592
BstMBI GATC 5 cut(s) 64, 79, 293, 556, 589
BstMWI GCNNNNNNNGC 3 cut(s) 323, 410, 656
BstNI CCWGG 1 cut(s) 382
BstSCI CCNGG 2 cut(s) 380, 438
BstSLI GKGCMC 1 cut(s) 486
BstUI CGCG 1 cut(s) 639
BstV1I GCAGC 1 cut(s) 637
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BsuRI GGCC 1 cut(s) 636
BtgI CCRYGG 1 cut(s) 637
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 642
Cac8I GCNNGC 2 cut(s) 149, 472
CfoI GCGC 1 cut(s) 476
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 525
Cfr42I CCGCGG 1 cut(s) 640
Csp6I GTAC 1 cut(s) 253
CviJI RGCY 8 cut(s) 116, 122, 151, 317, 353, 404, 443, 636
CviKI_1 RGCY 8 cut(s) 116, 122, 151, 317, 353, 404, 443, 636
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 2 cut(s) 405, 617
DpnI GATC 5 cut(s) 66, 81, 295, 558, 591
DpnII GATC 5 cut(s) 64, 79, 293, 556, 589
EaeI YGGCCR 1 cut(s) 634
EciI GGCGGA 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 354
Eco32I GATATC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 525
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoO109I RGGNCCY 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 380
EcoRV GATATC 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaiI YATR 9 cut(s) 93, 195, 257, 270, 276, 468, 479, 498, 629
FaqI GGGAC 1 cut(s) 380
Fnu4HI GCNGC 4 cut(s) 88, 637, 640, 651
FokI GGATG 1 cut(s) 289
Fsp4HI GCNGC 4 cut(s) 88, 637, 640, 651
FspBI CTAG 1 cut(s) 546
GlaI GCGC 1 cut(s) 475
GluI GCNGC 4 cut(s) 88, 637, 640, 651
GsuI CTGGAG 3 cut(s) 70, 138, 403
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 1 cut(s) 636
HapII CCGG 2 cut(s) 440, 471
HhaI GCGC 1 cut(s) 476
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HpaII CCGG 2 cut(s) 440, 471
HphI GGTGA 3 cut(s) 50, 119, 241
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 3 cut(s) 182, 334, 375
Hpy188III TCNNGA 2 cut(s) 49, 611
Hpy8I GTNNAC 1 cut(s) 484
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 145, 335
HpyCH4III ACNGT 3 cut(s) 33, 107, 599
HpyCH4IV ACGT 4 cut(s) 75, 251, 321, 519
HpyCH4V TGCA 4 cut(s) 197, 484, 653, 659
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 410, 656
HpyF3I CTNAG 2 cut(s) 405, 617
HpySE526I ACGT 4 cut(s) 75, 251, 321, 519
HspAI GCGC 1 cut(s) 474
KroI GCCGGC 1 cut(s) 470
KroNI GCCGGC 1 cut(s) 472
KspI CCGCGG 1 cut(s) 640
Kzo9I GATC 5 cut(s) 64, 79, 293, 556, 589
LmnI GCTCC 3 cut(s) 119, 278, 448
Lsp1109I GCAGC 1 cut(s) 637
LweI GCATC 1 cut(s) 121
MaeI CTAG 1 cut(s) 546
MaeII ACGT 4 cut(s) 75, 251, 321, 519
MaeIII GTNAC 2 cut(s) 38, 599
MalI GATC 5 cut(s) 66, 81, 295, 558, 591
MboI GATC 5 cut(s) 64, 79, 293, 556, 589
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 3 cut(s) 58, 283, 486
MluCI AATT 3 cut(s) 238, 450, 660
MmeI TCCRAC 2 cut(s) 378, 544
MnlI CCTC 9 cut(s) 78, 198, 354, 357, 380, 394, 400, 562, 598
MroNI GCCGGC 1 cut(s) 470
MseI TTAA 2 cut(s) 60, 664
MslI CAYNNNNRTG 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 639
MspI CCGG 2 cut(s) 440, 471
MspR9I CCNGG 2 cut(s) 382, 440
MvaI CCWGG 1 cut(s) 382
MvnI CGCG 1 cut(s) 639
MwoI GCNNNNNNNGC 3 cut(s) 323, 410, 656
NaeI GCCGGC 1 cut(s) 472
NciI CCSGG 1 cut(s) 440
NdeII GATC 5 cut(s) 64, 79, 293, 556, 589
NgoMIV GCCGGC 1 cut(s) 470
NlaIV GGNNCC 3 cut(s) 391, 444, 527
NmuCI GTSAC 1 cut(s) 38
PdiI GCCGGC 1 cut(s) 472
PflMI CCANNNNNTGG 1 cut(s) 21
PkrI GCNGC 4 cut(s) 89, 638, 641, 652
Ppu21I YACGTR 1 cut(s) 252
PpuMI RGGWCCY 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 380
PspGI CCWGG 1 cut(s) 380
PspN4I GGNNCC 3 cut(s) 391, 444, 527
PspPI GGNCC 1 cut(s) 525
PspPPI RGGWCCY 1 cut(s) 525
PsuI RGATCY 1 cut(s) 64
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
RseI CAYNNNNRTG 1 cut(s) 105
SacII CCGCGG 1 cut(s) 640
SaqAI TTAA 2 cut(s) 60, 664
SatI GCNGC 4 cut(s) 88, 637, 640, 651
Sau3AI GATC 5 cut(s) 64, 79, 293, 556, 589
Sau96I GGNCC 1 cut(s) 525
ScrFI CCNGG 2 cut(s) 382, 440
SduI GDGCHC 3 cut(s) 58, 283, 486
SetI ASST 9 cut(s) 78, 124, 153, 254, 312, 324, 411, 522, 564
SfaNI GCATC 1 cut(s) 121
Sfr303I CCGCGG 1 cut(s) 640
SgrBI CCGCGG 1 cut(s) 640
SinI GGWCC 1 cut(s) 525
SmiMI CAYNNNNRTG 1 cut(s) 105
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 3 cut(s) 238, 450, 660
SsiI CCGC 6 cut(s) 85, 88, 326, 489, 637, 639
SspMI CTAG 1 cut(s) 546
StyD4I CCNGG 2 cut(s) 380, 438
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 3 cut(s) 33, 107, 599
TaiI ACGT 4 cut(s) 78, 254, 324, 522
TaqI TCGA 5 cut(s) 78, 190, 213, 244, 417
TasI AATT 3 cut(s) 238, 450, 660
TauI GCSGC 3 cut(s) 90, 639, 642
Tru1I TTAA 2 cut(s) 60, 664
Tru9I TTAA 2 cut(s) 60, 664
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 1 cut(s) 38
TseI GCWGC 1 cut(s) 650
Tsp45I GTSAC 1 cut(s) 38
TspDTI ATGAA 3 cut(s) 89, 127, 354
TspRI CASTG 1 cut(s) 649
Van91I CCANNNNNTGG 1 cut(s) 21
VneI GTGCAC 1 cut(s) 482
VpaK11BI GGWCC 1 cut(s) 525
XagI CCTNNNNNAGG 1 cut(s) 358
XapI RAATTY 1 cut(s) 450
XcmI CCANNNNNNNNNTGG 2 cut(s) 256, 560
XspI CTAG 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.